Gambaran histologis folikel ovarium sapi aceh pascavitrifikasi menggunakan etilen glikol

  • Arman Sayuti Laboratorium Klinik Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh
  • Jamilatun Hidayah Program StudiPendidikan Dokter Hewan Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh
  • Muslim Akmal Laboratorium Biologi, Embriologi, danHistologiFakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh
  • Budianto Panjaitan Fakultas Kedokteran Hewan, Universitas Syiah Kuala, Banda Aceh
DOI: https://doi.org/10.29244/avl.3.1.3-4

Abstract

Penelitian ini bertujuan mengetahui gambaran histologis folikel ovarium sapi aceh pasca vitrifikasi menggunakan etilen glikol (EG) dengan berbagai konsentrasi berbeda yaitu 10%, 20% dan 30%. Phosphate buffered saline (PBS), 0.25 M sukrosa, 0.5 M sukrosa dan krioprotektan EG digunakan sebagai larutan vitrifikasi. Ovarium dipaparkan dengan larutan mengandung krioprotektan EG konsentrasi 10%, 20%, dan 30% selama 5 menit masing-masing pada suhu kamar, dikemas dalam straw, lalu divitrifikasi dalam nitrogen cair (-196 ⸰C), dan thawing dalam air pada 37 ⸰C. Kondisi normal dan keutuhan folikel pascavitrifikasi diamati secara histologi. Hasil pengamatan menunjukkan bahwa ovarium setelah vitrifikasi dengan EG 10% dan 20% memperlihatkan presentase keutuhan folikel 32.83% dan 45.04%, sedangkan EG 30% memperlihatkan presentase tertinggi yaitu 54.96%. Dari hasil penelitian ini dapat disimpulkan bahwa EG 30% lebih baik dalam mempertahankan keutuhan folikel ovarium sapi aceh dibandingkan dengan EG 10%  dan 20%.

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Published
2019-02-28
How to Cite
SayutiA., HidayahJ., AkmalM., & PanjaitanB. (2019). Gambaran histologis folikel ovarium sapi aceh pascavitrifikasi menggunakan etilen glikol. ARSHI Veterinary Letters, 3(1), 3-4. https://doi.org/10.29244/avl.3.1.3-4
Issue
Vol. 3 No. 1 (2019): ARSHI Veterinary Letters - Februari 2019
Section
Articles

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