Acute Toxicity Test and Development of Lacticaseibacillus rhamnosus MG5511 from Bima Horse Milk in the Intestine and Its Effects on Intestinal Mucosa from Probiotic Test Products

Abstract

Probiotics are viable microorganisms that promote health benefits by balancing positive microbes in the gut, and controlling pathogenic microbes in the digestive tract. Probiotic from genus Lactobacillus is the most widely used.  In addition to effectiveness testing, development and research on probiotics need to be supplemented with evidence from clinical and non-clinical data. This study was aimed to collect non-clinical data by examining the effectivity and toxicity of a probiotic candidate Lacticaseibacillus rhamnosus MG5511 from BIma horse milk (KRG) in the intestine and their effect on the intestinal mucosa. Animal study was conducted  with administration of the specific strain in MRS broth in 107 to 1010 CFU/mL concentrations to mice. Parameters observed were clinical signs, daily body weight measurement, gross organ pathology, and growth of bacterial populations. Based on the results, the strain Lacticaseibacillus rhamnosus MG5511 was able to survive the gastrointestinal tract for 14 days and can be classified as relatively harmless based on the results of toxicity test.