Multidrug-Resistant (MDR) E. coli dalam Daging Babi yang Berasal dari Rumah Potong Hewan (RPH) Oeba, Kota Kupang

Abstract

To produce pork ready for consumption, it must go through various stages, from the farm to the packaging. The chances of contamination with pathogenic microbes are very large. Pathogenic microbes are often found in pork, one of which is E. coli, which is often the trigger for foodborne disease in humans. Previous studies have shown that E. coli isolates from pig farms in Kupang City are known to have multidrug resistance (MDR), which, if this event spreads, could negatively impact the community. RPH is a place for slaughtering animals supervised by veterinarians to produce good quality meat. This study aims to measure E. coli contamination, identify and isolate MDR E. coli from fresh pork from Oeba RPH, Kupang City. The enumeration number of E. coli was carried out based on the Indonesian National Standard (SNI) ISO 16649–2:2001 concerning the Horizontal Enumeration Method for E. coli positive-b-glukuronidase - Part 2: Colony counting technique at 44 °C using 5-bromo-4-chloro-3-indolyl-b-D-glucuronide. Resistance test of E. coli to antibiotics was carried out based on the Kirby-Bauer method. Data related to the amount of E. coli contamination and testing of E.coli antibiotic resistance were analyzed descriptively. The results showed that the contamination of E. coli in the pork used had an average of 626,440 ± 947,937 CFU/g. All E. coli isolates were resistant to penicillin (100%), most E. coli isolates were resistant to streptomycin (80%) and tetracycline (60%), and half of the E. coli isolates were resistant to oxytetracycline (47%) and amoxicillin (40%). E. coli isolates obtained from pork had MDR against 2 (7/15;46.67%) to 3 (7/15;46.67%) groups of antibiotics. This study concludes that the contamination of E. coli in pork from Oeba slaughterhouse exceeds the maximum microbial contamination limit (>1x10¹ CFU/g).  E. coli isolates were resistant to penicillin, streptomycin, tetracycline, oxytetracycline, and amoxicillin and had MDR against 2 to 3 classes of antibiotics.