Short Communication: Detection Of The Serotype Of Avibacterium Paragallinarum From Indonesian Poultry Field Isolate Using Hemagglutination Inhibition Test

  • A.E.T.H. Wahyuni Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada
  • Fadhli Nanda Putra
  • Low Kar Yee Student of Undergraduate Program of Veterinary Science, Faculty of Veterinary Medicine, Universitas Gadjah Mada
  • Tan Yun Ru Student of Undergraduate Program of Veterinary Science, Faculty of Veterinary Medicine, Universitas Gadjah Mada
  • Cheng Ern Wei Student of Undergraduate Program of Veterinary Science, Faculty of Veterinary Medicine, Universitas Gadjah Mada
  • Yahya Pambudhi Student of Undergraduate Program of Veterinary Science, Faculty of Veterinary Medicine, Universitas Gadjah Mada
  • Puteri Nur Natasha Student of Undergraduate Program of Veterinary Science, Faculty of Veterinary Medicine, Universitas Gadjah Mada
DOI: https://doi.org/10.29244/avi...54-58
Keywords: Avibacterium paragallinarum, serotyping, hemagglutination inhibition, poultry field isolates

Abstract

Infectious coryza (IC) is an opportunistic and infectious upper respiratory tract disease in poultry, caused by Avibacterium paragallinarum. This disease has high morbidity but low mortality. The major losses caused by IC are the reduced egg production and increased feed conversion ratio (FCR). There are three recognized Avibacterium paragallinarum serovars, which are serovar A, B, and C. Limited reports regarding the serovars of Avibacterium paragallinarum in Indonesia are available. This research was done to detect the serovar of Avibacterium paragallinarum from Indonesian poultry field isolate using hemagglutination inhibiotion (HI) test. Ten field isolates of Avibacterium paragallinarum were re-identified, then processed into antigen solution using sonication, further on used for hemagglutination (HA) test. After the 4HA unit titer was obtained, the antigens were tested with HI using reference antisera (Strain 221 serovar A, strain Spross serovar B, and strain Modesto, serovar C). The results showed that one isolate had the highest titer with antiserum serovar A which was >5120 HI unit and nine isolates had the highest titer with antiserum serovar B which was >5120 HI Unit. Therefore, it can be concluded that one field isolates of Avibacterium paragallinarum from layer chicken is serovar A and nine field isolates of Avibacterium paragallinarum from layer, broiler, free range chicken, quail field isolates are serovar B.

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Published
2022-10-28
Issue
2022: Special Issues
Section
Penelitian / Research

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