Evaluasi Rekayasa Ekstraksi Biji, Minyak, dan Limbah Habbatussauda (Nigella sativa) terhadap Komponen Bioaktif dan Kemampuan Penghambatan Bakteri
Evaluation of the Extraction Process of Seeds, Oil, and Waste of Black Seed (Nigella sativa) on Bioactive Compound and Bacterial Inhibition Ability
DOI:
https://doi.org/10.29244/jintp.23.1.64-73Keywords:
ekstraksi bertingkat, fitobiotik, fitokimia, jintan hitam, thymoquinoneAbstract
The content of active compounds in natural materials is generally low and highly variable, thus requiring efficient and selective extraction methods. This study aims to evaluate a stepwise extraction process applied to the seeds, oil, and by-products of Nigella sativa (black cumin) to obtain thymoquinone (TQ) and other phytochemicals. The study consisted of four main stages: (1) extraction of HS oil using the cold press method, (2) stepwise extraction using 70% ethanol as solvent, (3) analysis of TQ content and phytochemical compounds in the extracts, and (4) evaluation of the antibacterial activity of habbatussauda oil extracts against pathogenic and non-pathogenic bacteria. Results showed that the cold press method yielded 34.46% oil. Stepwise extraction using 70% ethanol resulted in significant differences (p<0.05) in both yield and TQ content among the seed, oil, and by-product extracts. Among all samples, the ethanol extract from habbatussauda oil exhibited the highest yield and TQ concentration. Furthermore, at a concentration of 100 μg mL⁻¹, this extract inhibited the growth of pathogenic bacteria such as Salmonella typhimurium and Escherichia coli, without affecting non-pathogenic strains like Lactobacillus spp. and Bifidobacteria spp. In conclusion, stepwise extraction using 70% ethanol proved effective in enhancing TQ recovery and demonstrated potential as a phytobiotic agent with selective antibacterial activity against pathogenic bacteria.
Key words: black seed, phytobiotic, phytochemicals, stepwise extraction, thymoquinone
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References
Al-Saleh IA, Billedo G, & El-Doush II. 2006. Level of selenium, tocopherol, thymoquinone and thymol of Nigella sativa seed. Journal of Food Composition Analysis. 19:167-75.
Abdullah & Koc B. 2012. Kinetics of ultrasound-assited oil extraction from black seed (Nigella sativa). Journal of Food Processing and Preservation. 37(5):814-823.
Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, Damanhouri ZA & Anwar F. 2013. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pacific Journal of
Tropical Biomedicine. 3(5), 337–352.
Chaieb K, Kouidhi B, Jrah H, Mahdouani K & Bakhrouf A. 2011. Antibacterial activity of Thymoquinone, an active principle of Nigella sativa and its potency to prevent bacterial biofilm formation. BMC Complementary Medicine and Therapies. 13:11-29.
Chemat F, Rombaut N, Sicaire AG, Meullemiestre A, Fabiano-Tixier AS, Abert-Vian & M. 2017. Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrasonics Sonochemistry. 34: 540–560.
Eloff JN. 1998. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Medica. 64 : 711-716.
Epand RM, Walker C, Epand RF & Magarvey NA. 2016. Molecular mechanisms of membrane targeting antibiotics. Biochimica et Biophysica Acta. 1858: 980–987.
Imad H, Muhanned A, Aamera J & Cheah Y. 2014. Analysis of eleven Y-chromosomal STR markers in middle and south of Iraq. African Journal of Biotechnology. 13(38):3860-3871.
Kareem MA, Hussein AO & Hameed IH .2015. Y-chromosome short tandem repeat, typing technology, locus information and allele frequency in different population: A review. African Journal of Biotechnology. 14(27):2175-2178.
Kouidhi B, Zmantar T, Jrah H, Souiden Y, Chaieb K, Mahdouani K, Bakhrouf A. 2011. Antibacterian and resistence-modifying activities of thymoquinone against oral pathogens. Annals of Clinical Microbiology and Antimicrobials. 27:10-29.
Li P, Yin ZQ, Li SL, Huang XJ, Ye WC & Zhang QW. 2014. Simultaneous determination of eight flavonoids and pogostone in Pogostemon cablin by high performance liquid chromatography. Journal of Liquid Chromatography & Related Technologies. 37(12): 1771–1784.
Ma C, Liu C, Ahmed AF, Niu Y & Kang W. 2019. Optimum extraction technology for the seed oil of Nigella sativa L. 2019. Hindawi Journal of Food Quality. 7: 1-6.
Mahmud HA, Seo H, Kim S, Islam MI, Nam KW, Cho HD & Song HY. 2017. Thymoquinone (TQ) inhibits the replication of intracellular Mycobacterium tuberculosis in macrophages and modulates nitric oxide production. BMC Complementary Alternative Medicine. 17(1): 279-287.
Matthaus, B. and Brühl, L. 2003. Quality of cold–pressed edible rapeseed oil in Germany. Nahrung. 47(6):413–419.
Mingeot-Leclercq MP & Décout JL. 2016. Bacterial lipid membranes as promising targets to fight antimicrobial resistance, molecular foundations and illustration through the renewal of aminoglycoside antibiotics and emergence of amphiphilic aminoglycosides. Medicinal Chemistry Commmunications. 7:586–611.
Mohammed NK, Abd Manap MY, Tan CP, Muhialdin BJ, Alhelli AM & Hussin ASM. 2016. The Effects of Different Extraction Methods on Antioxidant Properties, Chemical Composition, and Thermal Behavior of Black Seed (Nigella sativa L.) Oil. Evidence-Based Complementary and Alternative Medicine. 1-10.
Nickavar B, Mojab F, Javidnia K, Amoli MA. 2003. Chemical composition of the fixed and volatile oils of Nigella sativa L. from Iran. Zeitschrift für Naturforschung C. 58(9-10): 629–631.
Retnani Y, Wiryawan KG, Khotijah L, Barkah NN, Gustian RA & Dermawan IR. 2019. Growth performance, blood metabolites and nitrogen utilization of lambs fed with Nigella sativa meal. Pakistan Journal of Nutrition. 18 (3): 247-253.
Steel RGD & Torrie JH. 1993. Prinsip dan Prosedur Statistika. Penerjemah Bambang Sumanti. Jakarta (ID): Gramedia Pustaka.
Tavares TD, Antunes JC, Padrão J, Ribeiro AI, Zille A, Amorim M, Ferreira F & Felgueiras HP. 2020. Activity of specialized biomolecules against gram-positive and gram-negative bacteria. Antibiotics (Basel). 9(6), 314-318.
Urbanova K, Kloucek P, Havlik J, Valterova I & Kokoska L. 2020. Fast and ecological liquid-liquid separation method for preparing quinones enriched extract from Nigella sativa oil. Journal of the Brazilian Chemical Society. 31(4): 739-745.
Woo CC, Kumar AP, Sethi G & Tan KHB. 2012. Thymoquinone: potential cure for inflammatory disorders and cancer. Biochemical Pharmacology. 83(4): 443–451.
Yi Y, Zhang QW, Li SL, Wang Y, Ye WC, Zhao J & Wang YT. 2012. Simultaneous quantification of major flavonoids in “Bawanghua”, the edible flower of Hylocereus undatus using pressurised liquid extraction and high performance liquid chromatography. Food Chemistry. 135(2): 528–533.
Zhang QW, Li GL, & Wen CY. 2018. Techniques for extraction and isolation of natural products: a comprehensive review. Chinese Medicine.13: 20-46.
Zhou YQ, Zhang QW, Li SL, Yin ZQ, Zhang XQ & Ye WC. 2012. Quality evaluation of semen oroxyli through simultaneous quantification of 13 components by high performance liquid chromatography. Currently Pharmacological Analysis. 8(2): 206–213.
Zuorro A, Lavecchia R, Medici F, & Piga L. 2014. Use of cell wall degrading enzymes for the production of high–quality functional products from tomato processing waste, Chemical Engineering Transactions. 38:355–360.
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