Sperm Kinematics and Morphology of Bali Bull (Bos javanicus) after Freezing and Thawing Treated with Green Tea Extract in Extender
Abstract
Green Tea Extract, or GTE, is a powerful antioxidant that can be added to the extender to maintain the quality of Bali bull semen before and after freezing. This study aimed to evaluate the impact of the GTE addition in the extender on the sperm kinematic and sperm morphology from frozen semen of Bali bull. Five Bali bulls from the NAIC, were used as semen sources. In this study, there were four different treatments: a control (Tris-egg yolk without GTE), a GTE0.05 (100 mL tris egg yolk with 0.05 mg of GTE), a GTE0.10 (100 mL tris egg yolk with 0.10 mg of GTE), and a GTE0.15 (100 mL tris egg yolk with 0.15 mg of GTE). A sperm analyzer (CASA) was used to observe the sperm kinematics and morphology. Compared to the control group, adding different concentrations of GTE to the semen extender did not affect the sperm’s morphology. Before freezing, adding GTE0.05 increases total and progressive motility as well as VAP, VCL, and VSL after thawing. GTE0.15 increases STR and ALH. The conclusion is that GTE additions at doses of 0.05 mg/100 mL and 0.15 mg/100 mL can increase sperm kinematics parameters, but sperm morphology is not affected by the GTE.
References
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Evangelista-Vargas, S. & A. Santiani. 2017. Detection of intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) and lipid peroxidation during cryopreservation of alpaca spermatozoa. Reprod. Domest. Anim. 52:819–824. https://doi.org/10.1111/rda.12984
Gadani, B., D. Bucci, M. Spinaci, C. Tamanini, & G. Galeati. 2017. Resveratrol and epigallocatechin-3-gallate addition to thawed boar sperm improves in vitro fertilization. Theriogenology 90:88–93. https://doi.org/10.1016/j.theriogenology.2016.11.020
Hezavehei, M., M. Sharafi, H. M. Kouchesfahani, R. Henkel, A. Agarwal, V. Esmaeili, & A. Shahverdi. 2018. Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches. Reprod. Biomed. Online 37:327–339. https://doi.org/10.1016/j.rbmo.2018.05.012
Hinrichs, K. & S. C. Loux. 2012. Hyperactivated sperm motility: Are equine sperm different? J. Equine Vet. Sci. 32:441–444. https://doi.org/10.1016/j.jevs.2012.05.070
Inanç, M. E., B. Çil, D. Yeni, F. Avdatek, D. Orakçi, P. B. Tuncer, R. Türkmen, & U. Taşdemir. 2019. The effect of green tea extract supplementation in bull semen cryopreservation. Kafkas Universitesi Veteriner Fakultesi Dergisi 25:703–708.
Kandiel, M. M., A. R. El-Khawagah, & K. G. Mahmoud. 2017. Effect of epidermal growth factor on buffalo frozen spermatozoa biometry and metabolic activity. Asian Pacific Journal Reproduction 6:43–48. https://doi.org/10.12980/apjr.6.20170108
Khattak, N. B. S., H. Khan, M. T. Tunio, S. Ahmad, N. Ahmad, S. M. H. Andrabi, O. Obaidullah, I. Ali, & N.-Z Fang. 2022. Dose-Dependent effect of green tea (Camellia sinensis) extract in the post-thawed fertility indicators of frozen bovine semen extender. Pak. J. Zoology 54:647–656. https://doi.org/10.17582/journal.pjz/20181230051216
Lavara, R., J. S. Vicente, & M. Baselga. 2013. Genetic variation in head morphometry of rabbit sperm. Theriogenology 80:313–318. https://doi.org/10.1016/j.theriogenology.2013.04.015
Len, J. S., W. S. D. Koh, & S.-X Tan. 2019. The roles of reactive oxygen species and antioxidants in cryopreservation. Biosci. Rep. 39:BSR20191601. https://doi.org/10.1042/BSR20191601
Mahmoudi, R., A. Azizi, S. Abedini, V. H. Jahromi, H. Abidi, M. J. Barmak, & M. J. Barmak. 2018. Green tea improves rat sperm quality and reduced cadmium chloride damage effect in spermatogenesis cycle. Journal Medicine Life 11:371–380. https://doi.org/10.25122/jml-2018-0005
Martí, J. I., I. M. Aparicio, C. L. V. Leal, & M. García-Herreros. 2012. Seasonal dynamics of sperm morphometric subpopulations and its association with sperm quality parameters in ram ejaculates. Theriogenology 78:528–541. https://doi.org/10.1016/j.theriogenology.2012.02.035
Martínez-Pastor, F., E. J. Tizado, J. J. Garde, L. Anel, & P. De Paz. 2011. Statistical series: Opportunities and challenges of sperm motility subpopulation analysis. Theriogenology 75:783–795. https://doi.org/10.1016/j.theriogenology.2010.11.034
Nasiri, A. H., A. Towhidi, & S. Zeinoaldini. 2012. Combined effect of DHA and α-tocopherol supplementation during bull semen cryopreservation on sperm characteristics and fatty acid composition: DHA improves sperm cryosurvival in bull. Andrologia 44:550–555. https://doi.org/10.1111/j.1439-0272.2011.01225.x
Park, S.-H. & I.-J Yu. 2017. Effect of antioxidant supplementation in freezing extender on porcine sperm viability, motility and reactive oxygen species. J. Anim. Reprod. Biotechnol. 32:9–15. https://doi.org/10.12750/JET.2017.32.1.9
Park, S.-H. & I.-J Yu. 2015. Evaluation of toxicity of green tea extract in chilled boar spermatozoa. J. Anim. Reprod. Biotechnol. 30:1–6. https://doi.org/10.12750/JET.2015.30.1.1
Pilane, C. M., M. A. Bopape, B. Ntombizodwa, & M. H. Mapeka. 2019. Buck semen does not easily succumb to oxidative stress. Open Journal Animal Sciences 09:65–75. https://doi.org/10.4236/ojas.2019.91006
Said, T. M., A. Gaglani, & A. Agarwal. 2010. Implication of apoptosis in sperm cryoinjury. Reprod. Biomed. Online 21:456–462. https://doi.org/10.1016/j.rbmo.2010.05.011
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Authors
PrastiyaR. A., DeboraA. E., WijayantiA., AgustonoB., SaputroA. L., AmaliaA., & SasiS. M. (2023). Sperm Kinematics and Morphology of Bali Bull (Bos javanicus) after Freezing and Thawing Treated with Green Tea Extract in Extender. Tropical Animal Science Journal, 46(4), 418-427. https://doi.org/10.5398/tasj.2023.46.4.418
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