Comparison of Different Lecithin Diluents for Cryopreservation of Toraya Buffalo Semen
Abstract
The objective of this study was to compare the quality of frozen semen from Toraya buffalo with different lecithins from different commercial diluents. Fresh semen from two 6-9 years old Toraya buffaloes were collected once a week in the morning through an artificial vagina. Fresh semen was examined macroscopically and microscopically. Semen with more than 70% sperm motility was divided into three tubes, each diluted with Andromed (soy lecithin), Steridyl (egg yolk lecithin), and Bovifree (synthetic lecithin) diluents at a concentration of 100 x106 mL-1 motile sperm. The diluted sperm were placed in 0.25 mL straws and allowed to equilibrate for 4 hours. The sperm was frozen above liquid nitrogen favour for 15 minutes and stored in liquid nitrogen containers for further evaluation. The quality of frozen semen was assessed 24 hours after freezing. The parameters tested were motility, viability, abnormalities, plasma membrane integrity, and sperm recovery rate (RR). The results showed no significant differences in the values of motility, viability, abnormalities, plasma membrane integrity, and sperm RR in the three diluents used. Sperm motility values ranged from 45.93% to 47.09% after freezing. Sperm viability ranged from 56.21% to 60.27%. The values of membrane integrity of the three diluents used ranged from 57.73% to 61.36%. The values of sperm abnormalities after freezing and thawing ranged from 2.74% to 3.18%. In conclusion, three commercial diluents containing animal, vegetable, and synthetic lecithin bases can be used as diluents for freezing Toraya buffalo semen with similar results.
References
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Chaudhari, D. V., A. J. Dhami, K. K. Hadiya, & J. A. Patel. 2015. Relative efficacy of egg yolk and soya milk-based extenders for cryopreservation (−196 °C) of buffalo semen. Vet. World 8:239–244. https://doi.org/10.14202/vetworld.2015.239-244
Dessouki, S., D. Ahmed, & A. Fayed. 2022. Sperm kinetics of egyptian buffalo bulls (Bubalus bubalis) affected by the red laser postfreezing. J. Adv. Vet. Anim. Res. 9:396. https://doi.org/10.5455/javar.2022.i607
Drobnis, E. Z., L. M. Crowe, T. Berger, T. J. Anchordoguy, J. W. Overstreet, & J. H. Crowe. 1993. Cold shock damage is due to lipid phase transitions in cell membranes: A demonstration using sperm as a model. J. Exp. Zool. 265:432–437. https://doi.org/10.1002/jez.1402650413
Emamverdi, M., M. Zhandi, Zare, A. Shahneh, M. Sharafi, & A. Akbari-Sharif. 2013. Optimization of ram semen cryopreservation using a chemically defined soybean lecithin-based extender. Reprod. Domest. Anim. 48:899–904. https://doi.org/10.1111/rda.12183
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Herbowo, M. T., R. I. Arifiantini, N. W. K. Karja, & R. G. Sianturi. 2020. Comparison of different cryoprotective agents on swamp buffalo semen cryopreservation. J. Indones. Trop. Anim. Agric. 45:268–276. https://doi.org/10.14710/jitaa.45.4.268-276
Indriastuti, R., M. F. Ulum, R. I. Arifiantini, & B. Purwantara. 2020. Individual variation in fresh and frozen semen of Bali bulls (Bos sondaicus). Vet. World 13:840–846. https://doi.org/10.14202/vetworld.2020.840-846
Iskandar, H., H. Sonjaya, R. I. Arifiantini, & H. Hasbi. 2022. The quality of fresh and frozen Semen and its correlation with molecular weight of seminal plasma protein in Bali cattle. Trop. Anim. Sci. J. 45:405–412. https://doi.org/10.5398/tasj.2022.45.4.405
Juyena, N. S. & C. Stelletta. 2012. Seminal plasma: An essential attribute to spermatozoa. J. Androl. 33:536–551. https://doi.org/10.2164/jandrol.110.012583
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Kumar, P., M. Saini, D. Kumar, A. K. Balhara, S. P. Yadav, P. Singh, & P. S. Yadav. 2015. Liposome-based semen extender is suitable alternative to egg yolk-based extender for cryopreservation of buffalo (Bubalus bubalis) semen. Anim. Reprod. Sci. 159:38–45. https://doi.org/10.1016/j.anireprosci.2015.05.010
Layek, S. S., T. K. Mohanty, A. Kumaresan, & J. E. Parks. 2016. Cryopreservation of bull semen: evolution from egg yolk based to soybean based extenders. Anim. Reprod. Sci. 172:1–9. https://doi.org/10.1016/j.anireprosci.2016.04.013
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Mehdipour, M., H. D. Kia, G. Moghaddam, & H. Hamishehkar. 2018. Effect of egg yolk plasma and soybean lecithin on rooster frozen-thawed sperm quality and fertility. Theriogenology 116:89–94. https://doi.org/10.1016/j.theriogenology.2018.05.013
Miguel-Jimenez, S., M. M. R. del Alamo, M. Álvarez-Rodríguez, C. O. Hidalgo, A. I. Peña, R. Muiño, J. E. Rodríguez-Gil, & T. Mogas. 2020. In vitro assessment of egg yolk-, soya bean lecithin- and liposome-based extenders for cryopreservation of dairy bull semen. Anim. Reprod. Sci. 215:1–12. https://doi.org/10.1016/j.anireprosci.2020.106315
Morrell, J. M. & M. Wallgren. 2014. Alternatives to antibiotics in semen extenders: A review. Pathogens 3:934–946. https://doi.org/10.3390/pathogens3040934
Mulia, B. H., A. Widianti, J. Manansang, D. R. Setiadi, V. T. Yoelinda, T. P. Nugraha, N. W. K. Karja, & R. I. Arifiantini. 2021. Establishment of semen collection technique using electroejaculator and semen cryopreservation of javan leopard (Panthera pardus melas Cuvier, 1809). Vet. World. 14:3156–3163. https://doi.org/10.14202/vetworld.2021.3156-3163
Namula, Z., F. Tanihara, M. Wittayarat, M. Hirata, N. T. Nguyen, T. Hirano, Q. A. Le, M. Nii, & T. Otoi. 2019. Effects of tris (hydroxymethyl) aminomethane on the quality of frozen-thawed boar spermatozoa. Acta Vet. Hung. 67:106–114. https://doi.org/10.1556/004.2019.012
Naz, S., M. Umair, & S. Iqbal. 2018. Comparison of tris egg yolk-based, triladyl® and optixell® extender on post-thaw quality, kinematics and in vivo fertility of nili ravi buffalo (Bubalus bubalis) bull spermatozoa. Andrologia 50:1–6. https://doi.org/10.1111/and.13063
Nikitkina, E., A. Musidray, A. Krutikova, P. Anipchenko, K. Plemyashov, & G. Shiryaev. 2020. Efficiency of tris-based extender steridyl for semen cryopreservation in stallions. Animals 10:1801. https://doi.org/10.3390/ani10101801
Nsairat, H., D. Khater, U. Sayed, F. Odeh, A. Al-Bawab, & W. Alshaer. 2022. Liposomes: Structure, composition, types, and clinical applications. Heliyon 8:e09394. https://doi.org/10.1016/j.heliyon.2022.e09394
Oke, M., J. Jacob, & G. Paliyath. 2010. Effect of soy lecithin in enhancing fruit juice/sauce quality. Food Res. Int. 43:232-240. https://doi.org/10.1016/j.foodres.2009.09.021
Rasyid, I., S. N. Sirajuddin, Hastang, & Nirwana. 2022. Characteristics of the striped buffalo (Tedong bonga) traders in bolu market animal, north toraja regency, south Sulawesi Province, Indonesia. Journal Positive School Psychology 6:1038–1042.
Rekha, A., B. F. Zohara, F. Bari, & M. G. S. Alam. 2016. Comparison of commercial triladyl extender with a tris-fructose-egg-yolk extender on the quality of frozen semen and pregnancy rate after transcervical AI in bangladeshi indigenous sheep (Ovis aries). Small Rumin. Res. 134:39–43. https://doi.org/10.1016/j.smallrumres.2015.12.007
Silvestre, M-A., V-D. Francisco, C-L. Guadalupe, C. Viridiana, G. Juan, & A. G. Oscar, 2021. Determinación de la calidad del semen criopreservado con lecitina de soya o yema de huevo, en machos cabríos. Abanico Veterinario 11:1–12. https://doi.org/10.21929/abavet2021.8
Singh, A., A. Kumar, M. Honparkhe, S. Kaur, H. Kaur, S. Ghuman, & P. Brar. 2018. Comparison of in vitro and in vivo fertilizing potential of buffalo bull semen frozen in egg yolk-, soya bean lecithin- and liposome-based extenders. Reprod. Domest. Anim. 53:195–202. https://doi.org/10.1111/rda.13092
Stewart, J. L., C. F. Shipley, A. S. Katich, E. Po, R. E. Ellerbrock, F. S. Lima, & I. F. Canisso. 2016. Cryopreservation of white-tailed deer (Odocoileus virginianus) semen using soybean-, liposome-, and egg yolk-based extenders. Anim. Reprod. Sci. 171:7–16. https://doi.org/10.1016/j.anireprosci.2016.05.006
Sukmawati, E., R. I. Arifiantini, & B. Purwantara. 2014. Daya tahan spermatozoa terhadap proses pembekuan pada berbagai jenis sapi pejantan unggul. Jurnal Ilmu Ternak Veteriner 19:168–175.
Swelum, A. A., I. M. Saadeldin, H. Ba-Awadh, M. G. Al-Mutary, A. F. Moumen, A. N. Alowaimer, & H. Abdalla. 2019. Efficiency of commercial egg yolk-free and egg yolk-supplemented tris-based extenders for dromedary camel semen cryopreservation. Animals 9:999. https://doi.org/10.3390/ani9110999
Tahar, M. A., Komariah, H. Nuraini, T. Maulana, M. Gunawan, & R. I. Arifiantini. 2022. Assessment of sperm acrosome status, malondialdehyde and aspartate aminotransferase enzyme concentration of frozen semen from limousin and simmental bulls in different commercial diluents. Jurnal Ilmu Ternak Veteriner 27:122–129.
Tarig, A. A., H. Wahid, Y. Rosnina, N. Yimer, Y. M. Goh, F. H. Baiee, A. M. Khumran, H. Salman, & M. Ebrahimi. 2017. Effect of different concentrations of egg yolk and virgin coconut oil in Tris-based extenders on chilled and frozen-thawed bull semen. Anim. Reprod. Sci. 182:21–27. https://doi.org/10.1016/j.anireprosci.2017.03.024
Yulnawati, M., Gunawan, H. Maheswari, M. Rizal, Herdis, & A. Boediono. 2010. Quality of epididymal and ejaculated sperms of spotted buffalo in dextrose supplemented extender. HAYATI J. Biosci. 17:27–30. https://doi.org/10.4308/hjb.17.1.27
Yusnizar, Y., M. Wilbe, A. O. Herlino, C. Sumantri, R. R. Noor, A. Boediono, L. Andersson, & G. Andersson. 2015. Microphthalmia‐associated transcription factor mutations are associated with white‐spotted coat color in swamp buffalo. Anim. Genet. 46:676–682. https://doi.org/10.1111/age.12334
Zhao, F., R. Li, Y. Liu & H. Chen. 2023. Perspectives on lecithin from egg yolk: Extraction, physicochemical properties, modification, and applications. Front. Nutr. 9:1082671. https://doi.org/10.3389/fnut.2022.1082671
Arif, A. A., T. Maulana, E. M. Kaiin, B. Purwantara, & R. I. Arifiantini. 2022. The quality of frozen semen of limousin bull in various semen diluents. Trop. Anim. Sci. J. 45:284–290. https://doi.org/10.5398/tasj.2022.45.3.284
[BSN] National Standardization Agency of Indonesia. 2021. Frozen Semen- part 2: Buffalo. SNI 4869-1:2021. National Standardization Agency of Indonesia, Jakarta. pp. 1-4.
Baharun, A., R. I. Arifiantini, & T. L. Yusuf. 2017. Freezing capability of pasundan bull sperm using tris-egg yolk, tris-soy, and andromed® diluents. Indonesian Journal Veterinary Sciences 11:45–49. https://doi.org/10.21157/j.ked.hewan.v11i1.5810
Bustani, G. S. & F. H. Baiee. 2021. Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders. Vet. World 14:1220–1233. https://doi.org/10.14202/vetworld.2021.1220-1233
Chaudhari, D. V., A. J. Dhami, K. K. Hadiya, & J. A. Patel. 2015. Relative efficacy of egg yolk and soya milk-based extenders for cryopreservation (−196 °C) of buffalo semen. Vet. World 8:239–244. https://doi.org/10.14202/vetworld.2015.239-244
Dessouki, S., D. Ahmed, & A. Fayed. 2022. Sperm kinetics of egyptian buffalo bulls (Bubalus bubalis) affected by the red laser postfreezing. J. Adv. Vet. Anim. Res. 9:396. https://doi.org/10.5455/javar.2022.i607
Drobnis, E. Z., L. M. Crowe, T. Berger, T. J. Anchordoguy, J. W. Overstreet, & J. H. Crowe. 1993. Cold shock damage is due to lipid phase transitions in cell membranes: A demonstration using sperm as a model. J. Exp. Zool. 265:432–437. https://doi.org/10.1002/jez.1402650413
Emamverdi, M., M. Zhandi, Zare, A. Shahneh, M. Sharafi, & A. Akbari-Sharif. 2013. Optimization of ram semen cryopreservation using a chemically defined soybean lecithin-based extender. Reprod. Domest. Anim. 48:899–904. https://doi.org/10.1111/rda.12183
Fleisch, A., E. Malama, U. Witschi, C. Leiding, M. Siuda, F. Janett, & H. Bollwein. 2017. Effects of an extension of the equilibration period up to 96 hours on the characteristics of cryopreserved bull semen. Theriogenology 89:255–262. https://doi.org/10.1016/j.theriogenology.2016.10.018
Herbowo, M. T., R. I. Arifiantini, N. W. K. Karja, & R. G. Sianturi. 2020. Comparison of different cryoprotective agents on swamp buffalo semen cryopreservation. J. Indones. Trop. Anim. Agric. 45:268–276. https://doi.org/10.14710/jitaa.45.4.268-276
Indriastuti, R., M. F. Ulum, R. I. Arifiantini, & B. Purwantara. 2020. Individual variation in fresh and frozen semen of Bali bulls (Bos sondaicus). Vet. World 13:840–846. https://doi.org/10.14202/vetworld.2020.840-846
Iskandar, H., H. Sonjaya, R. I. Arifiantini, & H. Hasbi. 2022. The quality of fresh and frozen Semen and its correlation with molecular weight of seminal plasma protein in Bali cattle. Trop. Anim. Sci. J. 45:405–412. https://doi.org/10.5398/tasj.2022.45.4.405
Juyena, N. S. & C. Stelletta. 2012. Seminal plasma: An essential attribute to spermatozoa. J. Androl. 33:536–551. https://doi.org/10.2164/jandrol.110.012583
Kaiin, E. M., M. Gunawan, & T. Maulana. 2017. Morphometry and abnormality evaluation of sex-sorted sperm of spotted buffalo (Tedong bonga). Nustantara Bioscience 9:175–180. https://doi.org/10.13057/nusbiosci/n090212
Kumar, P., M. Saini, D. Kumar, A. K. Balhara, S. P. Yadav, P. Singh, & P. S. Yadav. 2015. Liposome-based semen extender is suitable alternative to egg yolk-based extender for cryopreservation of buffalo (Bubalus bubalis) semen. Anim. Reprod. Sci. 159:38–45. https://doi.org/10.1016/j.anireprosci.2015.05.010
Layek, S. S., T. K. Mohanty, A. Kumaresan, & J. E. Parks. 2016. Cryopreservation of bull semen: evolution from egg yolk based to soybean based extenders. Anim. Reprod. Sci. 172:1–9. https://doi.org/10.1016/j.anireprosci.2016.04.013
Lima-Verde, I., A. Johannisson, T. Ntallaris, E. Al-Essawe, Z. Al-Kass, T. Nongbua, F. Dórea, N. Lundeheim, K. Kupisiewicz, & A. Edman. 2018. Effect of freezing bull semen in two non-egg yolk extenders on post-thaw sperm quality. Reprod. Domest. Anim. 53:127–136. https://doi.org/10.1111/rda.13080
Mehdipour, M., H. D. Kia, G. Moghaddam, & H. Hamishehkar. 2018. Effect of egg yolk plasma and soybean lecithin on rooster frozen-thawed sperm quality and fertility. Theriogenology 116:89–94. https://doi.org/10.1016/j.theriogenology.2018.05.013
Miguel-Jimenez, S., M. M. R. del Alamo, M. Álvarez-Rodríguez, C. O. Hidalgo, A. I. Peña, R. Muiño, J. E. Rodríguez-Gil, & T. Mogas. 2020. In vitro assessment of egg yolk-, soya bean lecithin- and liposome-based extenders for cryopreservation of dairy bull semen. Anim. Reprod. Sci. 215:1–12. https://doi.org/10.1016/j.anireprosci.2020.106315
Morrell, J. M. & M. Wallgren. 2014. Alternatives to antibiotics in semen extenders: A review. Pathogens 3:934–946. https://doi.org/10.3390/pathogens3040934
Mulia, B. H., A. Widianti, J. Manansang, D. R. Setiadi, V. T. Yoelinda, T. P. Nugraha, N. W. K. Karja, & R. I. Arifiantini. 2021. Establishment of semen collection technique using electroejaculator and semen cryopreservation of javan leopard (Panthera pardus melas Cuvier, 1809). Vet. World. 14:3156–3163. https://doi.org/10.14202/vetworld.2021.3156-3163
Namula, Z., F. Tanihara, M. Wittayarat, M. Hirata, N. T. Nguyen, T. Hirano, Q. A. Le, M. Nii, & T. Otoi. 2019. Effects of tris (hydroxymethyl) aminomethane on the quality of frozen-thawed boar spermatozoa. Acta Vet. Hung. 67:106–114. https://doi.org/10.1556/004.2019.012
Naz, S., M. Umair, & S. Iqbal. 2018. Comparison of tris egg yolk-based, triladyl® and optixell® extender on post-thaw quality, kinematics and in vivo fertility of nili ravi buffalo (Bubalus bubalis) bull spermatozoa. Andrologia 50:1–6. https://doi.org/10.1111/and.13063
Nikitkina, E., A. Musidray, A. Krutikova, P. Anipchenko, K. Plemyashov, & G. Shiryaev. 2020. Efficiency of tris-based extender steridyl for semen cryopreservation in stallions. Animals 10:1801. https://doi.org/10.3390/ani10101801
Nsairat, H., D. Khater, U. Sayed, F. Odeh, A. Al-Bawab, & W. Alshaer. 2022. Liposomes: Structure, composition, types, and clinical applications. Heliyon 8:e09394. https://doi.org/10.1016/j.heliyon.2022.e09394
Oke, M., J. Jacob, & G. Paliyath. 2010. Effect of soy lecithin in enhancing fruit juice/sauce quality. Food Res. Int. 43:232-240. https://doi.org/10.1016/j.foodres.2009.09.021
Rasyid, I., S. N. Sirajuddin, Hastang, & Nirwana. 2022. Characteristics of the striped buffalo (Tedong bonga) traders in bolu market animal, north toraja regency, south Sulawesi Province, Indonesia. Journal Positive School Psychology 6:1038–1042.
Rekha, A., B. F. Zohara, F. Bari, & M. G. S. Alam. 2016. Comparison of commercial triladyl extender with a tris-fructose-egg-yolk extender on the quality of frozen semen and pregnancy rate after transcervical AI in bangladeshi indigenous sheep (Ovis aries). Small Rumin. Res. 134:39–43. https://doi.org/10.1016/j.smallrumres.2015.12.007
Silvestre, M-A., V-D. Francisco, C-L. Guadalupe, C. Viridiana, G. Juan, & A. G. Oscar, 2021. Determinación de la calidad del semen criopreservado con lecitina de soya o yema de huevo, en machos cabríos. Abanico Veterinario 11:1–12. https://doi.org/10.21929/abavet2021.8
Singh, A., A. Kumar, M. Honparkhe, S. Kaur, H. Kaur, S. Ghuman, & P. Brar. 2018. Comparison of in vitro and in vivo fertilizing potential of buffalo bull semen frozen in egg yolk-, soya bean lecithin- and liposome-based extenders. Reprod. Domest. Anim. 53:195–202. https://doi.org/10.1111/rda.13092
Stewart, J. L., C. F. Shipley, A. S. Katich, E. Po, R. E. Ellerbrock, F. S. Lima, & I. F. Canisso. 2016. Cryopreservation of white-tailed deer (Odocoileus virginianus) semen using soybean-, liposome-, and egg yolk-based extenders. Anim. Reprod. Sci. 171:7–16. https://doi.org/10.1016/j.anireprosci.2016.05.006
Sukmawati, E., R. I. Arifiantini, & B. Purwantara. 2014. Daya tahan spermatozoa terhadap proses pembekuan pada berbagai jenis sapi pejantan unggul. Jurnal Ilmu Ternak Veteriner 19:168–175.
Swelum, A. A., I. M. Saadeldin, H. Ba-Awadh, M. G. Al-Mutary, A. F. Moumen, A. N. Alowaimer, & H. Abdalla. 2019. Efficiency of commercial egg yolk-free and egg yolk-supplemented tris-based extenders for dromedary camel semen cryopreservation. Animals 9:999. https://doi.org/10.3390/ani9110999
Tahar, M. A., Komariah, H. Nuraini, T. Maulana, M. Gunawan, & R. I. Arifiantini. 2022. Assessment of sperm acrosome status, malondialdehyde and aspartate aminotransferase enzyme concentration of frozen semen from limousin and simmental bulls in different commercial diluents. Jurnal Ilmu Ternak Veteriner 27:122–129.
Tarig, A. A., H. Wahid, Y. Rosnina, N. Yimer, Y. M. Goh, F. H. Baiee, A. M. Khumran, H. Salman, & M. Ebrahimi. 2017. Effect of different concentrations of egg yolk and virgin coconut oil in Tris-based extenders on chilled and frozen-thawed bull semen. Anim. Reprod. Sci. 182:21–27. https://doi.org/10.1016/j.anireprosci.2017.03.024
Yulnawati, M., Gunawan, H. Maheswari, M. Rizal, Herdis, & A. Boediono. 2010. Quality of epididymal and ejaculated sperms of spotted buffalo in dextrose supplemented extender. HAYATI J. Biosci. 17:27–30. https://doi.org/10.4308/hjb.17.1.27
Yusnizar, Y., M. Wilbe, A. O. Herlino, C. Sumantri, R. R. Noor, A. Boediono, L. Andersson, & G. Andersson. 2015. Microphthalmia‐associated transcription factor mutations are associated with white‐spotted coat color in swamp buffalo. Anim. Genet. 46:676–682. https://doi.org/10.1111/age.12334
Zhao, F., R. Li, Y. Liu & H. Chen. 2023. Perspectives on lecithin from egg yolk: Extraction, physicochemical properties, modification, and applications. Front. Nutr. 9:1082671. https://doi.org/10.3389/fnut.2022.1082671
Authors
RiwuR. M. J., ArifiantiniR. I., & KarjaN. W. K. (2023). Comparison of Different Lecithin Diluents for Cryopreservation of Toraya Buffalo Semen. Tropical Animal Science Journal, 46(4), 396-402. https://doi.org/10.5398/tasj.2023.46.4.396
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