This study aims to examine the polymorphisms of the IGF-1 gene associated with body weight and size. A total of 60 sheep from different farm locations in Palu City (Villages of Taipa, Poboya, Kawatuna, and Petobo), Central Sulawesi, Indonesia, were used. The variables observed were adult body weight and measurements, such as shoulder height, body length, humerus length, radius-ulna length, metacarpus length, hip height, femur length, tibial length, and metatarsus length as phenotypic characteristics. IGF-1 gene polymorphisms were analyzed using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) with Bsp143ǀǀ as a restriction enzyme. These gene polymorphisms were calculated using the allele and genotype frequency approach, as well as the Polymorphic Informative Content (PIC). Genotype associations with body weight and bone size were analyzed using the General Linear Model analysis method. The PCR-RFLP analysis results showed the presence of polymorphisms with the frequency and genotypes of GG (21.7%), GC (68.3%), and CC (10%). Furthermore, the frequencies of G (55.8%) and C (44.2%) genes with a 49.32% polymorphism rate (PIC) were not in the Hardy-Weinberg equilibrium (p<0.05). In conclusion, the IGF-1 gene polymorphism was detected in Palu sheep with an insignificant effect on body weight and bone size.
Badan Pusat Statistik Provinsi Sulawesi Tengah. 2015. Provinsi Sulawesi Tengah dalam Angka. URL: http://sulteng.bps.go.id/frontend/linkTabelStatis/view/id/100
Budisatria, I. G. S., H. M. J. Udo, C. H. A. M. Eilers, E. Baliarti, & A. J. van der Zijpp. 2010. Preferences for sheep or goats in Indonesia. Small Rumin. Res. 88:16–22. https://doi.org/10.1016/j.smallrumres.2009.11.002
Curi, R. A., H. N. de Oliveira, A. C. Silveira, & C. R. Lopes. 2005. Association between IGF-I, IGF-IR, and GHRH gene polymorphisms and growth and carcass traits in beef cattle. Livest. Prod. Sci. 94:159–167. https://doi.org/10.1016/j.livprodsci.2004.10.009
Darwish, H. R., H. M. El-Shorbagy, A. M. Abou-Eisha, A. E. El-Din, & I. M. Farag. 2017. New polymorphism in the 5′ flanking region of IGF-1 gene and its association with wool traits in Egyptian Barki sheep. J. Genet. Eng. Biotechnol. 15:437–441. https://doi.org/10.1016/j.jgeb.2017.08.001
Dickson, M. C., J. C. Saunders, & R. S. Gilmour. 1991. The ovine insulin-like growth factor-I gene: Characterization, expression, and identification of a putative promoter. J. Mol. Endocrinol. 6:17–31. https://doi.org/10.1677/jme.0.0060017
Eric, S. D., Nicholas, T. K. D. D., & Theophilus, K. A. 2014. Bioinformatics with basic local alignment search tool (BLAST) and fast alignment (FASTA). J. Bioinform. Seq. Anal. 6:1–6. https://doi.org/10.5897/IJBC2013.0086
Farajallah, A., C. Sumantri, & U. Fauzi. 2007. The variation of microsatellite DNA among fat, medium and thin tail local sheep. Jurnal Protein 14:1-8.
Gholibeikifard, A., M. Aminafshar, & M. H. Mashhadi. 2013. Polymorphism of IGF-I and ADRB3 genes and their association with growth traits in the Iranian Baluchi sheep. J. Agric. Sci. Technol. 15:1153-1162.
Grochowska, E., B. Borys, P. Janiszewski, J. Knapik, & S. Mroczkowski. 2017. Effect of the IGF-I gene polymorphism on growth, body size, carcass and meat quality traits in Coloured Polish Merino sheep. Arch. Anim. Breed. 60:161–173. https://doi.org/10.5194/aab-60-161-2017
Gunawan, A., C. Sumantri, & R. Juniarti. 2017. Gen dan Keragaman Genetik Ternak (English: Livestock Genes and Genetic Diversity). IPB Press, Indonesia.
Haile, A., S. Gizaw, T. Getachew, J. P. Mueller, P. Amer, M. Rekik, & B. Rischkowsky. 2019. Community-based breeding programmes are a viable solution for Ethiopian small ruminant genetic improvement but require public and private investments. J. Anim. Breed. Genet. 136:319–328. https://doi.org/10.1111/jbg.12401
Peraturan Menteri Pertanian Nomor :19/Permentan /Ot.140/2/2010 Tentang Pedoman Umum Program Swasembada Daging Sapi 2014, (2010). (Government Regulations)
Malewa, A. D. 2014. Sifat kualitatif eksternal domba Donggala pada tiga lokasi di Sulawesi Tengah. Agrisains 10:101–109.
Malewa, A. D, L. Hakim, S. Maylinda, & M. H. Husain. 2014. Growth hormone gene polymorphisms of Indonesia fat-tailed sheep using PCR-RFLP and their relationship with growth traits. Livest. Res. Rural. Dev. 26: 115.
Molotsi, A., B. Dube, S. Oosting, T. Marandure, C. Mapiye, S. Cloete, & K. Dzama. 2017. Genetic traits of relevance to sustainability of smallholder sheep farming systems in South Africa. Sustainability 9:1225. https://doi.org/10.3390/su9081225
Montossi, F., M. Font-i-Furnols, M. del Campo, R. San Julián, G. Brito, & C. Sañudo. 2013. Sustainable sheep production and consumer preference trends: Compatibilities, contradictions, and unresolved dilemmas. Meat Sci. 95:772–789. https://doi.org/10.1016/j.meatsci.2013.04.048
Moradian, C., G. Esmailnia, & A. Hajihosseinlo. 2013. Polymorphism of IGF-1 gene in Makoei Sheep using PCR-SSCP. Eur. J. Exp. Biol. 3:490–494.
Nazari, F., A. Noshary, & B. Hemati. 2016. Association between insulin–like growth factor I polymorphism and early growth traits in Iranian Zandi sheep, found Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP). Iran. J. Appl. Anim. Sci. 6:665–669.
Putra, B. W., Nurhidayat, & C. Sumantri. 2012. Morphometric performances of thin thail sheep with differences calpastatin (CAST-1) genotypes. Proceeding of the 2nd International Seminar on Animal Industry, Jakarta, 5-6 july 2012. p. 135-139.
Petrović, M. P., M. M. Petrović, D. Ružić-Muslić, V. Caro-Petrović, N. Maksimović, Z. Ilić, & S. Vučković. 2011. Opportunities and challenges for sustainable sheep production in Serbia. Biotechnol. Anim. Husb. 27:463–472. https://doi.org/10.2298/BAH1103463P
Raji, A. O., A. Mohammed, J. U. Igwebuike, & C. Alphonsus. 2018. Association of IGF 1 gene polymorphisms with some morphometric traits of Nigerian indigenous sheep breeds. Nig. J. Biotech. 34:97-104. https://doi.org/10.4314/njb.v34i1.13
Sabbioni, A., V. Beretti, P. Superchi, & M. Ablondi. 2020. Body weight estimation from body measures in Cornigliese sheep breed. Ital. J. Anim. Sci. 19:25–30. https://doi.org/10.1080/1828051X.2019.1689189
Sambrook, J., E. F. Fritsch, & T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Sankhyan, V., Y. P. Thakur, & P. K. Dogra. 2020. Genetic polymorphism in IGF-1 gene in four sheep and goat breeds and its association with biometrical traits in migratory Gaddi goat breed of western Himalayan state of Himachal Pradesh, India. Indian J. Anim. Res. 54:508-512. https://doi.org/10.18805/ijar.B-3795
Sebastiano, L., M. M. Consuelo, D. S. M. Veronica, P. Luisa, C. Giovanni, N. Michella, & C. Vincenzo. 2020. Polymorphism of insulin-like growth factor 1 gene and its relationship with reproductive performances and milk yield in Sarda dairy sheep. Vet. Anim. Sci. 9:100084. https://doi.org/10.1016/j.vas.2019.100084
Tsehay, S., R. Ortiz, E. Johansson, E. Bekele, K. Tesfaye, C. Hammenhag, & M. Geleta. 2020. New transcriptome-based SNP markers for Noug (Guizotia abyssinica) and their conversion to KASP markers for population genetics analyses. Genes 11:1373. https://doi.org/10.3390/genes11111373
Tyasi, T. L., Y. F. Tyasi, A. L. Tyasi, S. Lagu, & S. Ngambu. 2015. A study of relationship between body weight and morphological traits by using path analysis in South African indigenous sheep. J. Biol. Agric. Healthc. 5:1–5.
Udo, H. M. J. & I. G. S. Budisatria. 2011. Fat-tailed sheep in Indonesia; an essential resource for smallholders. Trop. Anim. Health Prod. 43:1411–1418. https://doi.org/10.1007/s11250-011-9872-7
Widiati, R. & T. A. Kusumastuti. 2017. Production system and economic potential of the bligon local goat farming in Girimulyo Village, Panggang Sub District of Gunungkidul. Sains Peternakan 15:59. https://doi.org/10.20961/sainspet.v15i2.12374
Wong, K.-C., J. Zhang, S. Yan, X. Li, Q Lin, S. Kwong, & C. Liang. 2019. DNA sequencing technologies: sequencing data protocols and bioinformatics tools. ACM Comput. Surv. 52:98:1–98:30. https://doi.org/10.1145/3340286
WuJun, L., F. GuangXin, F Yi, T. KeChuan, H. XiXia, Y. XinKui, W. Mou, Y. Hui, H. YongZhen, X. JingJing, X. YaPing, Y. ShiGang, & C. Hong. 2010. The polymorphism of a mutation of IGF-1 gene on two goat breeds in China. J. Anim. Vet. Adv. 9:790–794. https://doi.org/10.3923/javaa.2010.790.794
Yousefi, S. & M. A. Azari. 2012. Genetic effect of growth hormone gene on yearling weight and wool traits in Zel sheep (Brief Report). Arch. Anim. Breed. 55:303–306. https://doi.org/10.5194/aab-55-303-2012
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors submitting manuscripts should understand and agree that copyright of manuscripts of the article shall be assigned/transferred to Tropical Animal Science Journal. The statement to release the copyright to Tropical Animal Science Journal is stated in Form A. This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA) where Authors and Readers can copy and redistribute the material in any medium or format, as well as remix, transform, and build upon the material for any purpose, but they must give appropriate credit (cite to the article or content), provide a link to the license, and indicate if changes were made. If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.