Effects of Supplementing Laying Hens with Purified Amino Acid Prepared from Animal Blood
Abstract
The objective of the experiment was to investigate the effects of supplementing laying hens with purified amino acids (PAA) derived from the blood of animal slaughter house on their egg production, egg quality, and immune response. The experiment was based on completely randomized design. A total of 144 Isa Brown laying hens (56-weeks old) were randomly allotted to 4 treatments with 4 pen replications (control, T1: 0.05%, T2: 0.1%, and T3: 0.5% PAA). Each pen housed nine laying hens. The laying hens were reared under a deep litter system. Once a week, a total of 12 eggs of each treatment were collected for egg quality analysis. At the end of the experiment, blood samples were collected for biochemical analysis and cytokines profiles using ELISA kit assays. Hen day egg production and hen house egg production increased significantly (P<0.05) along with the increase in PAA supplementation. The average egg weight increased significantly (P<0.05) with PAA supplementation. Significant differences (P<0.05) were also found in egg shell strength, shell thickness, and albumen ratio. Blood biochemical variables, such as glucose, total cholesterol, blood urea nitrogen (BUN), total bilirubin, and glutamate oxalate transaminase (GOT) were within the normal range. However, the glucose was highest in the control group. Whereas, the total cholesterol and total bilirubin were highest in T2 as compared to control group. Plasma immunoglobulin A (IgA) and immunoglobulin G (IgG), interleukin-1 (IL-1), interleukin-2 (IL-2) concentrations were not affected by PAA supplementation. Plasma interferon gamma (IFNγ) of PAA supplemented treatment groups was significantly (P<0.05) lower than the control group. However, tumor necrosis factor alpha (TNFα) was significantly (P<0.05) higher in T2. On the basis of these results, we conclude that PAA supplementation improved the production performance of laying hens without affecting their health.
References
https://doi.org/10.4236/fns.2012.34074
Adhikari, B. B., M. Chae, & D.C. Bressler. 2018. Utilization of slaughterhouse waste in value-added applications: recent advances in the development of wood adhesives. Polymers. 10: 176. https://doi.org/10.3390/polym10020176
Alagawany, M., M.A. El-Hack, M.R. Farag, R. Tiwari, S. Sachan, K. Karthik, & K. Dhama. 2016. Positive and negative impacts of dietary protein levels in laying hens. Asian J. Anim. Sci.10:165-174. https://doi.org/10.3923/ajas.2016.165.174
Ambardekar, A. A., R. C. Reigh, & M. B. Williams. 2009. Absorption of amino acids from intact dietary proteins and purified amino acid supplements follows different time-courses in channel catfish (Ictalurus punctatus). Aquaculture. 291:179-187. https://doi.org/10.1016/j.aquaculture.2009.02.044
Bah, C.S., A.E.D.A. Bekhit, A.Carne, & M. A. McConnell. 2013. Slaughterhouse blood: an emerging source of bioactive compounds. Compr. Rev. Food Sci. Food Saf. 12: 314-331. https://doi.org/10.1111/1541-4337.12013
Baker, D.H. 2009. Advances in protein–amino acid nutrition of poultry. Amino Acids 37: 29-41. https://doi.org/10.1007/s00726-008-0198-3
Bunchasak, C., K. Poosuwan, R. Nukraew, K. Markvichitr, & A. Choothesa. 2005. Effect of dietary protein on egg production and immunity responses of laying hens during peak production period. Int. J. Poult. Sci. 15:701-708. https://doi.org/10.3923/ijps.2005.701.708
Deng, W., X. F. Dong, J. M. Tong, & Q. Zhang. 2012. The probiotic Bacillus licheniformis ameliorates heat stress-induced impairment of egg production, gut morphology, and intestinal mucosal immunity in laying hens. Poult. Sci. 91: 575-582. https://doi.org/10.3382/ps.2010-01293
Fearon, J., S.B. Mensah, & V. Boateng. 2014. Abattoir operations, waste generation and management in the Tamale metropolis: Case study of the Tamale slaughterhouse. J. Public Health Epidemiol. 6: 14-19. https://doi.org/10.5897/JPHE2013.0574
Hanusova, E., C. Hrnčár, A. Hanus, & M. Oravcova. 2015. Effect of breed on some parameters of egg quality in laying hens. Acta Fytotechn Zootechn. 18: 20-24.
https://doi.org/10.15414/afz.2015.18.01.12-24
Harms, R.H. & G.B. Russell. 1993. Optimizing egg mass with amino acid supplementation of a low-protein diet. Poult. Sci. 72:1892-1896. https://doi.org/10.3382/ps.0721892
Haugh, R.R. 1937. The Haugh unit for measuring egg quality. United States Egg and Poultry Magazine. 43: 522-555.
Hejnfelt, A. & I. Angelidaki. 2009. Anaerobic digestion of slaughterhouse by-products. Biomass & bioenergy. 33: 1046-1054. https://doi.org/10.1016/j.biombioe.2009.03.004
Houston, D. C., D. Donnan, & P. J. Jones. 1995. The source of the nutrients required for egg production in zebra finches Poephila guttata. J. Zoology. 235:469-483. https://doi.org/10.1111/j.1469-7998.1995.tb01763.x
Jayathilakan, K., K. Sultana, K. Radhakrishna, & A. S. Bawa. 2012. Utilization of byproducts and waste materials from meat, poultry and fish processing industries: a review. J Food Sci. Technol. 49: 278-293. https://doi.org/10.1007/s13197-011-0290-7
Kebreab. E., J. France, R. P. Kwakkel, S. Leeson, H. D. Kuhi, & J. Dijkstra. 2009. Development and evaluation of a dynamic model of calcium and phosphorus flows in layer. Poult. Sci. 88: 680-689. https://doi.org/10.3382/ps.2008-00157
Keshavarz, K. & R. E. Austic. 2004. The use of low-protein, low-phosphorus, amino acid-and phytase-supplemented diets on laying hen performance and nitrogen and phosphorus excretion. Poult. Sci. 83:75-83. https://doi.org/10.1093/ps/83.1.75
Kingori, A.M., J.K. Tuitoek, H. K. Muiruri, & A. M. Wachira. 2010. Effect of dietary crude protein levels on egg production, hatchability and post-hatch offspring performance of indigenous chickens. Int. J. Poult. Sci. 9: 324-329. https://doi.org/10.3923/ijps.2010.324.329
Li, X., R. Rezaei, P. Li, & G. Wu. 2011. Composition of amino acids in feed ingredients for animal diets. Amino Acids. 40: 1159-1168. https://doi.org/10.1007/s00726-010-0740-y
Liu, Y. 2015. Fatty acids, inflammation and intestinal health in pigs. J. Anim. Sci. Biotech. 6: 41. https://doi.org/10.1186/s40104-015-0040-1
Melesse, A., W. Tiruneh, & T. Negesse. 2011. Effects of feeding Moringa stenopetala leaf meal on nutrient intake and growth performance of Rhode Island Red chicks under tropical climate. Trop. Subtrop. Agroecosyst.14: 485-492.
Melesse, A., Z. Worku, & Y. Teklegiorgis. 2013. Assessment of the prevailing handling and quality of eggs from scavenging indigenous chickens reared in different agro-ecological zones of Ethiopia. J. Environt. Occup. Sci. 2: 1-8. https://doi.org/ 10.5455/jeos.20130104091334.
Mohammed, K.A.E.F., L. Sarmiento-Franco, R. Santos-Ricalde, & J. F. Solorio-Sanchez. 2012. The nutritional effect of Moringa oleifera fresh leaves as feed supplement on Rhode Island Red hen egg production and quality. Trop. Anim. Health Prod. 44: 1035-1040. https://doi.org/10.1007/s11250-011-0037-5
Moreki, J.C. & B. Tiroesele. 2012. Termites and earthworms as potential alternative sources of protein for poultry. Intern. J. Agro. Veter. Med. Sci. 6: 368-376. https://doi.org/10.5455/ijavms.174
Novak, C., H. Yakout, & S. Scheideler. 2004. The combined effects of dietary lysine and total sulfur amino acid level on egg production parameters and egg components in Dekalb Delta laying hens. Poult. Sci. 83: 977-984. https://doi.org/10.1093/ps/83.6.977
Ofori, J.A. & Y. H. P. Hsieh. 2014. Issues related to the use of blood in food and animal feed. Critical Reviews Food Sci. Nutr. 54: 687-697. https://doi.org/10.1080/10408398.2011.605229
Oke, O.E., A.O. Ladokun, & O. M. Onagbesan. 2014. Quality parameters of eggs from chickens reared in deep litter system with or without access to grass or legume pasture. Magnesium (mg/g). 1: 2-12.
Osada, T., R. Takada, & I. Shinzato. 2011. Potential reduction of greenhouse gas emission from swine manure by using a low-protein diet supplemented with synthetic amino acids. Anim. Feed Sci. Technol. 166: 562-574. https://doi.org/10.1016/j.anifeedsci.2011.04.079
Pavlík, A., M. Lichovníková, & P. Jelínek. 2009. Blood plasma mineral profile and qualitative indicators of the eggshell in laying hens in different housing systems. Acta Veterinaria Brno. 78: 419-429. https://doi.org/10.2754/avb200978030419
Peganova, S. & K. Eder. 2002. Studies on requirement and excess of isoleucine in laying hens. Poult. Sci. 81: 1714-1721.https://doi.org/10.1093/ps/81.11.1714
Peganova, S. & K. Eder. 2003. Interactions of various supplies of isoleucine, valine, leucine and tryptophan on the performance of laying hens. Poult. Sci. 82: 100-105. https://doi.org/10.1093/ps/82.1.100
Ramsay, S. L. & D. C. Houston. 1998. The effect of dietary amino acid composition on egg production in blue tits. Proc. Biol. Sci. 265: 1401-1405. https://doi.org/10.1098/rspb.1998.0448
Ravindran, V. & W. L. Bryden. 1999. Amino acid availability in poultry—In vitro and in vivo measurements. Aust. J. Agric. Res. 50: 889-908. https://doi.org/10.1071/AR98174
Roberts, J.R. 2004. Factors affecting egg internal quality and egg shell quality in laying hens. J. Poult. Sc. 41:161-177. https://doi.org/10.2141/jpsa.41.161
Şekeroǧlu, A. & E. Altuntaş. 2009. Effects of egg weight on egg quality characteristics. J. Sci. Food Agric. 89: 379-383. https://doi.org/10.1002/jsfa.3454
Shafer, D. J., J. B. Carey, & J. F. Prochaska. 1996. Effect of dietary methionine intake on egg component yield and composition. Poult. Sci. 75: 1080-1085. https://doi.org/10.3382/ps.0751080
Sklan, D. & Y. Noy. 2004. Catabolism and deposition of amino acids in growing chicks: effect of dietary supply. Poult Sci. J. 83: 952-961. https://doi.org/10.1093/ps/83.6.952
Tabeekh, M. A. S. A. 2011. Evaluation of some external and internal egg quality traits of quails reared in Basrah City. Basra J. Vet. Res. 10: 78-84.
Tenesa, M., T. C. Loh, H. L. Foo, A. A. Samsudin, R. Mohamad, & A. R. Raha. 2016. Effects of feeding different levels of low crude protein diets with different Levels of amino acids supplementation on layer hen performance. Pertanika J. Trop. Agric. Sc. 39:543-555.
Torrallardona, D. & J. Polo. 2016. Effect of spray-dried porcine plasma protein and egg antibodies in diets for weaned pigs under environmental challenge conditions. J. Swine Health Prod. 24: 21-28.
Wandita, T.G., N. Joshi, I.S. Nam, S. H.Yang, S. H. Park, & S. G. Hwang. 2018. Dietary supplementation of purified amino acid derived from animal blood on immune response and growth performance of broiler chicken. Trop. Anim. Sci. J. 41:108-113. https://doi.org/10.5398/tasj.2018.41.2.108
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