Body Resistance and Growth Performance of Broiler Fed Glucomannan Extracted from Amorphophallus onchophyllus Tuber
Abstract
The purpose of the study was to examine the supplementation effects of glucomannan extracted from a porang tuber (GEPT) on body resistance and growth performance of broiler chickens. A total number of 160 one-day-old broiler chickens with the average body weight of 42.39±0.58 g was kept for 35 days. The study was arranged in a completely randomized design with 5 treatments and 4 replications (8 birds each). The dietary treatments were T0= basal ration, T1= basal ration with the addition of 0.05% GEPT, T2= basal ration with the addition of 0.1% GEPT, T3= basal ration with the addition of 0.15% GEPT, and T4= basal ration with the addition of 0.2% GEPT. Parameters measured were the population of lactic acid bacteria (LAB), Coliform count, relative weight of lymphoid organs (bursa fabricius and spleen), heterophils-lymphocytes (H/L) ratio, body weight gain, feed consumption, feed conversion ratio, and mortality. Data were subjected to analysis of variance (ANOVA) and continued with Duncan Multiple Range Test (DMRT) at 5% probability. The results showed that supplementation of the diets with GEPT significantly increased (P<0.05) LAB population, decreased (P<0.05) Coliform count in the jejunum and ileum, and H/L ratio, but did not affect the relative weight of lymphoid organs, and growth performance. In conclusion, diet added with 0.1% GEPT improved the balance of intestinal microflora and increased body resistance, without any negative effects on the lymphoid organs and growth performance of broiler chickens.
References
Bogusławska-Tryk, M., R. Szymeczko, A. Piotrowska, K. Burlikowska, & K. Slizewska. 2015. Ileal and cecal microbial population and short-chain fatty acid profile in broiler chickens fed diets supplemented with lignocellulose. Pak. Vet. J. 35:212-216.
Bolton, W. 1967. MAFF Bulletin. No.174. Poultry Nutrition. HMSO, London.
Bozkurt, M., K. Kucukyilmaz, A. U. Catli, M. Cinar, E. Bintas, & F. Coven. 2012. Performance, egg quality, and immune response of laying hens fed diets supplemented with mannan-oligosaccharide or an essential oil mixture under moderate and hot environmental conditions. Poult. Sci. 91:1379-1386. https://doi.org/10.3382/ps.2011-02023
Bukhari, S. M., M. Iram, T. Lijie, M. Sunting, H. L. Mang, G. Abbas, I. Baboo, & Y. Li. 2017. Coherence and colonization characteristics of recombinant Lactobacillus under simulated gastric conditions within chicken GI tract and its impact on chicken growth. Pak. Vet. J. 37:381-386.
Chauhan, P. S., N. Puri, P. Sharman, & N. Gupta. 2012. Mannasases: microbial sources, production, properties and potential biotechonological applications. Appl. Microbiol. Biotechnol. 93: 1817-1830. https://doi.org/10.1007/s00253-012-3887-5
Elrayeh, A. S. & G. Yildiz. 2012. Effects of inulin and β-glucan supplementation in broiler diets on growth performance, serum cholesterol, intestinal length, and immune system. Turk. J. Vet. Anim. Sci. 36:388-394.
Fajrih, N., N. Suthama, & V. D. Yunianto. 2014. Body resistance and productive performances of crossbred local chicken fed inulin of Dahlia tubers. Med. Pet. 37:108-114. https://doi.org/10.5398/medpet.2014.37.2.108
Fardiaz, S. 1993. Analisis Mikrobiologi Pangan. Raja Grafindo Persada, Jakarta.
Gross, W. B. & H. S. Siegel. 1983. Evaluation of heterophil/lymphocyte ratio as a measure of stress in chickens. Avian Dis. 27:972-979. https://doi.org/10.2307/1590198
Harmayani, E., V. Aprilia, & Y. Marsono. 2014. Characterization of glucomannan from Amorphophallus oncophyllus and its prebiotic activity in vivo. Carbohydr. Polym. 112:475–479. https://doi.org/10.1016/j.carbpol.2014.06.019
Hosseini, S. M., H. Nazarizadeh, S. Ahani, & M. V. Azghandi. 2016. Effects of mannan oligosaccharide and Curcuma xanthorrhiza essential oil on the intestinal morphology and stress indicators of broilers subjected to cyclic heat stress. Arch. Anim. Breed. 59:285–291. https://doi.org/10.5194/aab-59-285-2016
Houshmand, M., K. Azhar, I. Zulkifli, M. H. Bejo, & A. Kamyab. 2012. Effects of prebiotic, protein level, and stocking density on performance, immunity and stress indicators of broilers. Poult. Sci. 91:393–401. https://doi.org/10.3382/ps.2010-01050
Kermanshahi, H., M. D. Shakouri, & A. Daneshmand. 2018. Effect of non-starch polysaccharides in semi-purified diet on performance, serum metabolites, gastrointestinal morphology and microbial population of male broiler chickens. Livest. Sci. 214:93-97. https://doi.org/10.1016/j.livsci.2018.04.012
Kim, G. B., Y. M. Seo, C. H. Kim, & I. K. Paik. 2011. Effect of dietary prebiotic supplementation on the performance, intestinal microflora, and immune response of broilers. Poult. Sci. 90:75–82. https://doi.org/10.3382/ps.2010-00732
Kohl, K. D. 2012. Diversity and function of the avian gut microbiota. J. Comp. Phys. B. 182:591–602. https://doi.org/10.1007/s00360-012-0645-z
Li, S. P., X. J. Zhao, J. Y. Wang. 2009. Synergy of Astragalus polysaccharides and probiotics (Lactobacillus and Bacillus cereus) on immunity and intestinal microbiota in chicks. Poult. Sci. 88:519-25. https://doi.org/10.3382/ps.2008-00365
Mellata, M., K. Ameiss, H. Mo, & R. Curtiss. 2010. Characterization of the contribution to virulence of three large plasmids of avian pathogenic Escherichia coli χ7122 (O78:K80:H9). Infect. Immun. 78:1528–1541. https://doi.org/10.1128/IAI.00981-09
Markovi, R., D. Sefer, M. Krstic, & B. Petrujkic. 2009. Effect of different growth promoters on broiler performance and gut morphology. Arch. Med. Vet. 41:163-169. https://doi.org/10.4067/S0301-732X2009000200010
Mateova, S., J. Saly, M. Tuckova, J. Koscova, R. Nemcova, M. Gaalova, & D. Baranova. 2008. Effect of probiotics, prebiotics and herb oil on performance and metabolic parameters of broiler chickens. Medycyna Wet. 64:294-297.
Meimandipour, A., A. Soleimanifarjam, K. Azhar, M. Hair-Bejo, M. Shuhaimi, Leyla Nateghi, & A. M. Yazid. 2011. Age effects on short chain fatty acids concentrations and pH values in the gastrointestinal tract of broiler chickens. Arch. Geflugelk. 75:164-168.
Mikkelson, A., H. Maaheimo, & T. K. Hakala. 2013. Hydrolysis of konjac glucomannan by Trichoderma reesei mannanase and endoglucanases Cel7B and Cel5A for the production of glucomannooligosaccharides. Carbohydr. Research. 372:60-68. https://doi.org/10.1016/j.carres.2013.02.012
Mokoena, M. P. 2017. Lactic acid bacteria and their bacteriocins: classification, biosynthesis and appliccations against uropatogens. Mol. 22:1-13. https://doi.org/10.3390/molecules22081255
NRC. 1994. Nutrient Requirements of Poultry. 9th revised ed. National Academic Press, Washington DC.
Philip, P., D. Kern, J. Goldmanns, F. Seiler, A. Schulte, T. Habicher & J. Buchs. 2018. Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask. Microb. Cell Fact. 17:1-17. https://doi.org/10.1186/s12934-018-0917-8
Pourabedin, M. & Z. Zhao. 2015. Prebiotics and gut microbiota in chickens. FEMS Microbiol. Lett. 362:1-8. https://doi.org/10.1093/femsle/fnv122
Pourabedin, M, L. Guan & X. Zhao. 2015. Xylo-oligosaccharides and virginiamycin differentially modulate gut microbial composition in chickens. Microbiome 3:1-12. https://doi.org/10.1186/s40168-015-0079-4
Rebole, A., L. T. Ortiz, M. L. Rodríguez, C. Alzueta, J. Treviño, & S. Velasco. 2010. Effects of inulin and enzyme complex, individually or in combination, on growth performance, intestinal microflora, cecal fermentation characteristics, and jejunal histomorphology in broiler chickens fed a wheat- and barley-based diet. Poult. Sci. 89:276–286. https://doi.org/10.3382/ps.2009-00336
Ruminska, E., A. Koncicki, & T. Stenzel. 2008. Structure and function of the avian immune system in birds. Medycyna Wet. 64:265-268.
Sarangi, N. R., L. K. Babu,A. Kumar, C. R. Pradhan, P. K. Pati, & J. P. Mishra. 2016. Effect of dietary supplementation of prebiotic, probiotic, and synbiotic on growth performance and carcass characteristics of broiler chickens. Vet. World. 9:313-319. https://doi.org/10.14202/vetworld.2016.313-319
Saeed, M., F. Ahmad, M. A. Arain, M. E. Abd El-Hack, M. Emam, Z. A. Butto, & A. Moshaveri. 2017. Use of mannan-oligosaccharides (MOS) as a feed additive in poultry nutrition. J. World Poult. Res. 7:94-103.
Sellaoui, S., N. Alloui, S. Mehenaoui, & S. Djaaba. 2012. Evaluation of immune status of the chicken using morphometry and histology of the bursa of fabrisius. J. Vet. Adv. 2:440-443.
Sethy, K., S. K. Mishra, P. P. Mohanty, J. Agarawal, P. Meher, D. Satapathy , J. K. Sahoo, S. Panda, & S. M. Nayak. 2015. An overview of non starch polysaccharide. J. Anim. Nutr. Physiol. 1:17-22.
Shini, S., P. Kaiser, A. Shini, & W. L. Bryden. 2008. Biological response of chickens (Gallus gallus domesticus) induced by corticosterone and a bacterial endotoxin. Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 149:324-333. https://doi.org/10.1016/j.cbpb.2007.10.003
Steel, R. G. D, & J. H. Torrie. 1991. Prinsip dan Prosedur Statistika Suatu Pendekatan Biometrik. 2nd ed. Terjemahan. PT Gramedia Pustaka Tama, Jakarta.
Tester, R. F. & F. H. Al-Ghazzewi. 2013. Mannans and health, with a special focus on glucomannans. Food Res. Int. 50:384-391. https://doi.org/10.1016/j.foodres.2012.10.037
Wright, A. V. & L. Axelsson. 2011. Lactic Acid Bacteria: An Introduction. In: S. Lahtinne, S. Salminen, A. Von Wright & A. Ouwehand, Eds., Lactic Acid Bacteria: Microbiological and Functional Aspects. CRC Press, London. pp. 1-17. https://doi.org/10.1201/b11503
Yang, Y., P. A. Iji & M. Choct. 2009. Dietary modulation of gut microflora in broiler chickens: a review of the role of size kinds of altrnatives to in-feed antibiotics. Worlds Poult. Sci. J. 65:97-114. https://doi.org/10.1017/S0043933909000087
Yanuriati, A., D.W. Marseno, Rochmadi, & E. Harmayani. 2017. Characteristics of glucomannan isolated from fresh tuber of Porang (Amorphophallus muelleri Blume). Carbohydr. Polym. 156:56-63. https://doi.org/10.1016/j.carbpol.2016.08.080
Zhang, Y.Q., B. J. Xie, & X. Gan. 2005. Advance in the applications of konjac glucomannan and its derivatives, Carbohydr. Polym. 60:27–31. https://doi.org/10.1016/j.carbpol.2004.11.003
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