Nutritional improvement of free-gluten arrowroot biscuits through catfish flour enrichment Peningkatan gizi biskuit tepung garut bebas gluten melalui penambahan tepung ikan lele
Abstract
The demand for gluten-free products is increasing along with the growing awareness of gluten intolerance and sensitivity. The use of alternative flours, such as local tubers and fish flour fortification techniques, can help improve the nutritional content of gluten-free products. This study aims to determine the best concentration of catfish flour based on the nutritional content and hedonics of arrowroot flour-based biscuits. A completely randomized design (CRD) was used with four concentrations of catfish flour addition (0, 2, 4, and 6%). The analyses conducted were proximate tests, texture, and color. The research results indicate that the addition of 6% catfish flour is the best treatment, with biscuit characteristics including moisture content of 5.59%, ash 1.63%, protein 8.57%, fat 28.46%, and carbohydrates 55.39%. The biscuits with the addition of 6% catfish flour have a texture of 3.43 N, a color of L* 70.82, a* 2.46, and b* 37.69 and meet SNI 2973:2022 for protein parameters. Hedonic tests show that the addition of catfish flour to biscuits is acceptable and liked by the panelists. Overall, catfish flour is beneficial in enhancing the nutritional content of gluten-free biscuits made from arrowroot flour. These biscuits have the potential to become nutritious gluten-free snacks.
References
Abdel-Mobdy, H.E., Abdel-Aal, H.A., Souzan, S.L., Nassar, A.G. (2021). Nutritional value of African catfish (Clarias gariepinus) meat. Asian J. Appl. Chem. Res, 8, 31–39. https://doi.org/10.9734/ajacr/2021/v8i230190
Abraha, B., Mahmud, A., Admassu, H., Yang, F., Tsighe, N., Girmatsion, M., Xia, W., Magoha, P., Yu, P., Jiang, Q., & Xu, Y. (2018). Production and quality evaluation of biscuit incorporated with fish fillet protein concentrate. Journal of Nutrition & Food Sciences, 8(6), 1-13. https://doi.org/10.4172/2155-9600.1000744
Amante, Regina, P., Santos, E.C.Z., Correia, V.T.D., & Fante, C.A. (2021). Research notes: benefits and possible food applications of arrowroot (Maranta arundinaceae l.). Journal of Culinary Science & Technology, 19(6), 513–521.https://www.tandfonline.com/doi/full/10.1080/15428052.2020.1791295.
Andayani, Noor, S., Sitepu, G.S.B., Budiarta, I.N., and Made Lia Damayanti. (2022). Karakterisasi kimia dan sensori cookies non-gluten dengan subtitusi tepung tulang ikan tongkol (Euthynnus Affinis) sebagai alternatif makanan ringan penderita celiac.” Jurnal Sains dan Teknologi, 11(2), 257–66. https://doi.org/10.23887/jstundiksha.v11i2.45983.
Arvianto, A. A., Swastawati, F., & Wijayanti, I. (2016). Pengaruh fortifikasi tepung daging ikan lele dumbo (Clarias Gariepinus) terhadap kandungan asam amino lisin pada biskuit. Jurnal Pengolahan dan Bioteknologi Hasil Perikanan, 5(4), 20-25.
Asikin, A.N., Kusumaningrum, I., & Hidayat, T. (2024). Chemical characteristics of butter cookies with knife-fish (Chitalla sp.) bone flour addition as a healthy snack of high calcium. Food Research, 8(2), 78–83.
Astiana, I., Lahay, A. F., Utari, S. P. S. D., Farida, I., Samanta, P. N., Budiadnyani, I. G. A., & Febrianti, D. (2023). Karakteristik organoleptik dan nilai gizi biskuit dengan fortifikasi tepung surimi ikan swanggi (Priacanthus tayenus). Jurnal Pengolahan Hasil Perikanan Indonesia, 26(1), 107-116. http://dx.doi.org/10.17844/jphpi.v26i1.44286
Atteno, M., Costa, L., Cozzolino, A., Tortora, R., Caso, F., Del Puente, A., Cantarini, L., Scarpa, R., & Ciacci, C. (2014). The enthesopathy of celiac patients: effects of gluten-free diet. Clinical Rheumatology, 33(4), 537–41. https://doi.org/10.1007/s10067-014-2534-1
[AOAC]. (2016). Association of official analytical chemyst. 20th ed. rockville, USA: AOAC International.
Bakhtiar, B., Rohaya, S., & Ayunda, H. M. A. (2019). Penambahan tepung tulang ikan bandeng (Chanos chanos) sebagai sumber kalsium dan fosfor pembuatan donat panggang. Jurnal Teknologi dan Industri Pertanian Indonesia, 11(1), 38–45. https://doi.org/10.17969/jtipi.v11i1.13439
Bakare, A. H., Adeola, A. A., Otesile, I., Obadina, A.O., Afolabi, W.A., Adegunwa, M.O., Akerele, R.A., Bamgbose, O.O., & Alamu, E.O. (2020). Nutritional, texture, and sensory properties of composite biscuits produced from breadfruit and wheat flours enriched with edible fish meal. Food Science & Nutrition, 8(11), 6226-6246. https://doi.org/10.1002/fsn3.1919
Brites, L.T.G.F., Schmiele, M., & Steel, C.J. (2018). Gluten-free bakery and pasta products. Alternative and Replacement Foods, 385-410. https://doi.org/10.1016/b978-0-12-811446-9.00013-7
Bourne, & Malcolm, C. (2002). Food Texture and Viscosity : Concept and Measurement 2nd Edition. Elsevier Science & Technology Books
Dick, M., Limberger, C., Thys, R.C.S., De Oliveira Rios, A., & Flôr, S.H. (2020). Mucilage and cladode flour from cactus (Opuntia monacantha) as alternative ingredients in gluten-free crackers. Food Chemistry, 314, 126178. https://doi.org/10.1016/j.foodchem.2020.126178
Ermiş, E., Tuğla, B.K., & Külsoy, B. (2020). Effects of adding eggshell powder and olive seed powder to biscuit formulation on some quality properties. Turkish Journal of Agriculture - Food Science and Technology, 8(12), 2512–17. https://doi.org/10.24925/turjaf.v8i12.2512-2517.2609
Fathonah, S., Rosidah, Amalia, B., & Humaizah, S. (2019, August 19). The formulation of alternative gluten-free mung bean cookies [Conference Session]. Journal Of Physics: Conference Series, Volume 1444, The 8th Engineering International Conference 2019 16 August 2019, Semarang, Indonesia. Iop Conference Series: Earth and Environmental Science. https://doi.org/10.1088/1742-6596/1444/1/012004
Gambuś, H., Gambuś, F., Pastuszka, D., Wrona, P., Ziobro, R., Sabat, R., Mickowska, B., Nowotna, A., & Sikora, M. (2009). Quality of gluten-free supplemented cakes and biscuits.International Journal of Food Sciences and Nutrition, 60(4), 31–50. https://doi.org/10.1080/09637480802375523
Husain, R., Umar, N. S., & Suherman, S. P. (2023). Formulasi tepung ikan bandeng (Chanos chanos) dalam pembuatan biskuit sebagai makanan pendamping ASI (MP-ASI). Jambura Fish Processing Journal, 5(1), 47–59. https://doi.org/10.37905/jfpj.v5i1.15786
Irmayanti, & Fitriyana, L. (2019). Fortification of yellow potatoes sweet flour and jamblang fruit paste (Syzygiumcumini) in the making of cookies on it’s sensoric characteristics and mineral content. Journal of Physics: Conference Series, 1232(1), 012008. https://doi.org/10.1088/1742-6596/1232/1/012008
Istifada, D. S., Swastawati, F., & Wijayanti, I. (2023). Pengaruh penambahan tepung ikan teri hitam (Stolephorus insularis) terhadap karakteristik kimia dan tekstur pizza base. Jurnal Pengolahan Hasil Perikanan Indonesia, 26(2), 229-240. http://dx.doi.org/10.17844/jphpi.v26i2.44748
Kaimudin, M., Sumarsana, Radiena, M. S. Y., & Noto, S. H. (2021). Karakteristik pangan fungsional nuget dan stik dari tepung ikan layang ekor merah (Decapterus kuroides) dan ampas tahu. Jurnal Pengolahan Hasil Perikanan Indonesia, 24(3), 370-380.
Khotimah, K., Affandi, D. R., Nuary, R. B., Sanjaya, P. A., & Sulistiowati, M. (2023). Karakteristik biskuit kelor (Moringa oliefera) berbasis pati garut (Maranta arundinacea) dan tepung kacang merah (Phaseolus vulgaris L.) dengan variasi pemanis. Journal of Applied Agriculture, Health, and Technology, 1(2), 60–74. https://doi.org/10.20961/jaht.v1i2.559
Kohli, D., Jain, A., Singh, O., & Kumar, S. (2023). Gluten free approach of biscuits preparation. Journal of Agriculture and Food Research, 14, 100683. https://doi.org/10.1016/j.jafr.2023.100683
El Khoury, D., Balfour-Ducharme, S., & Joye, I.J. (2018). A review on the gluten-free diet: technological and nutritional challenges. Nutrients, 10(10), 1410.
Herrera-Quintana, L., Navajas-Porras, B., Vázquez-Lorente, H., Hinojosa-Nogueira, D., Corrales-Borrego, F.J., Lopez-Garzon, M., and Plaza-Diaz, J. (2025). Celiac disease: beyond diet and food awareness. Foods, 14(3), 1–25. https://doi.org/10.3390/foods14030377
Kristanti, D., Setiaboma, W., & Herminiati, A. (2020). Karakteristik fisikokimia dan organoleptik cookies mocaf dengan penambahan tepung tempe.Biopropal Industri,11(1), 1-8. https://doi.org/10.36974/jbi.v11i1.5354
Litaay, C., Mutiara, T. A., Indriati, A., Novianti, F., Nuraini, L., & Rahman, N. (2023). Fortifikasi tepung ikan teri (Stolephorus sp.) terhadap karakteristik fisik dan mikrostruktur mi berbasis sagu. Jurnal Pengolahan Hasil Perikanan Indonesia, 26(1), 127-138. http://dx.doi.org/10.17844/jphpi.v26i1.45159
Lestari, L.A., Huriyati, E., and Marsono, Y. (2017). The development of low glycemic index cookie bars from foxtail millet (Setaria Italica), arrowroot (Maranta Arundinacea) flour, and kidney beans (Phaseolus Vulgaris). Journal of Food Science and Technology, 54(6), 1406–13. https://doi.org/10.1007/s13197-017-2552-5
Manik, D.I.B., Nuryanto, B. Panunggal, and D. N. Afifah. (2020). Formulation of complementary feeding biscuits with yellowfin fish flour (Thunnus Albacares) and terubuk flour (Saccharum Edule Hasskarl) to improve nutritional status of children in Papua. Food Research, 4(2), 31–37. https://doi.org/10.26656/fr.2017.4(S3).S08
Marta, H., febiola, C., Cahyana, Y., Arifin, H.R., Fetriyuna, F., & Sondari, D. (2023). Application of composite flour from indonesian local tubers in gluten-free pancakes. Foods, 12(9), 1892. https://doi.org/10.3390/foods12091892
Maulani, A., Poernomo, A., Lisyana, H., & Sumandiarsa, I.K. (2024). Karakteristik gluten free cookies fortifikasi hidrolisat protein ikan lele. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan, 19(1), 73. https://doi.org/10.15578/jpbkp.v19i1.991
Mervina, C. M. K., & Mariiyati, S. A. (2012). Formulasi biskuit dengan substitusi tepung ikan lele dumbo (Clarias Gariepinus) dan isolat protein kedelai (Glycine Max) sebagai makanan potensial untuk anak balita gizi kurang. Jurnal Teknologi dan Industri Pangan, 23(1), 9–16.
Muhlishoh, A., Putri, N. A., & Ma’rifah, B. (2024). Formulasi cookies lidah kucing substitusi tepung ikan gabus dan tepung kacang hijau sebagai alternatif PMT balita gizi kurang. Jurnal Riset Gizi, 12(2), 217-232.
Muslihudin, Sidik, U., Liviawaty, E., Rochima, E., &Junianto. (2021). Study of preferred level of cookies fortified with nilem fish egg protein concentrate. Asian Journal of Fisheries And Aquatic Research, 10–17. https://doi.org/10.9734/ajfar/2021/v13i430270
Muzaki, I., Suprapto, H., & Kusdarwati, R. (2021, Sept 11). The substitution effect of bone fish flour milkfish (Chanos chanos) physical and chemical characteristics of cookies.[Conference Session]. The 1st International Conference on Biotechnology and Food Sciences, Surabaya, Indonesia. IOP Conference Series: Earth And Environmental Science. https://doi.org/10.1088/1755-1315/679/1/012044
Navia, Z.I., Suwardi, A.B., & Seprianto. (2021, Sept 11). Acceptability and test the levels of protein in a biscuit with the substitution of milkfish flour (Chanos chanos forskal) [Conference Session]. Proceedings of The 2nd International Conference on Science, Technology, and Modern Society (ICSTMS 2020). Advances In Social Science, Education and Humanities Research. Https://Doi.Org/10.2991/Assehr.K.210909.051
Padalino, L., Conte, A., & Del Nobile, M.A. (2016). Overview on the general approaches to improve gluten-free pasta and bread. Foods, 5(4), 87. https://doi.org/10.3390/foods5040087.
Pradipta, D. R. E., & Andoyo, R. (2020, July 29-30). Optimization formulation of high protein biscuit made from denaturated whey protein concentrate and sweet potato flour supplemented with mineral as emergency food [Conference Session]. International Conference on Food and Bio-Industry 2019, 29-30 July 2019, Bandung, Indonesia. IOP Conference Series: Earth and Environmental Science, 443(1), 01206 https://doi.org/10.1088/1755-1315/443/1/012066
Rasulu, H., Rodianawati, I., Hasbullah, Albaar, N., Umalekhoa, I., & Kamaluddin, A.K.. (2021, Okt 18-21). Physicochemical properties of sago flour food bars fortified with white sweet potato flour and sidat fish flour [Conference Session]. 7th International Conference on Sustainable Agriculture, Food and Energy 18-21 October 2019, Phuket, Thailand. IOP Conference Series: Earth and Environmental Science, 709(1), 012053. https://doi.org/10.1088/1755-1315/709/1/012053
Rodrigues Lbo, Veloso Cm, Bonomo Rcf, Rodrigues Lb, Minim La, & Costa Ras. (2018). Rheological and textural studies of arrowroot (maranta arundinacea) starch–sucrose–whey protein concentrate gels.Journal of Food Science and Technology,55(8), 2974-2984. https://doi.org/10.1007/s13197-018-3215-x
Romani, S., Rocculi, P., Tappi, S., and Dalla Rosa, M. (2016). Moisture adsorption behaviour of biscuit during storage investigated by using a new dynamic dewpoint method. Food Chemistry, 195, 97–103. https://doi.org/10.1016/j.foodchem.2015.06.114
Rosida, D.F., Putri, N.A., and Oktafiani, M. (2020). Karakteristik cookies tepung kimpul termodifikasi (Xanthosoma sagittifolium) dengan penambahan tapioka. Agrointek, 14(1), 45–56. https://doi.org/10.21107/agrointek.v14i1.6309
Saputra, F., Rochima, E., Apriliani, I. M., & Rostini, I. (2024). Pengaruh fortifikasi tepung tulang ikan patin (Pangasius sp.) terhadap peningkatan kalsium dan preferensi donat. Jurnal Pengolahan Hasil Perikanan Indonesia, 27(12), 1211-1218. http://dx.doi.org/10.17844/jphpi.v27i12.57118
Setyarini, D., Bustami, & Santoso, J. (2024). Chemical characteristics and functional properties of fish protein concentrate (FPC) and catfish bone meal. Jurnal Pengolahan Hasil Perikanan Indonesia, 27(6), 459–73. https://doi.org/10.17844/jphpi.v27i6.50064
Sibian, M.S., & Riar, C.S. (2020). Formulation and characterization of cookies prepared from the composite flour of germinated kidney bean, chickpea, and wheat. Legume Science, 2(3), 1-12. https://doi.org/10.1002/leg3.42
Sinthusamran, S., Benjakul, S., Kijroongrojana, K., & Prodpran, T.. (2019). Chemical, physical, rheological and sensory properties of biscuit fortified with protein hydrolysate from cephalothorax of pacific white shrimp. Journal of Food Science and Technology, 56(3),1145–54. https://doi.org/10.1007/s13197-019-03575-2.
Siswanti, Delinda, M. V., Parnanto, N. H. R., & Widowati, D. (2024). Karakteristik biskuit bayi dengan penambahan tepung jagung manis, tepung kacang merah, dan tepung ikan gabus. Jurnal Pengolahan Hasil Perikanan Indonesia, 27(4), 266–81. https://doi.org/10.17844/jphpi.v27i4.47665
Sudaryati, E., Nasution, E., and Ardiani, F. (2018). Nutritional quality of bread from mixture of arrowroot flour (Marantha Arundinacea L.) and wheat flour. Advances in Health Sciences Research, 9, 186–89. https://doi.org/10.2991/phico-17.2018.17
Victor-Aduloju, A. T., Chukwu, C. U., Okoli, Q. I., Innocent, A. F., Nwokolo, M. L., Chindimma, O. U. M., & Egbeonele, M. C. (2024). Functional and proximate composition of biscuit produced from wheat (Triticum Aestivum) flour, soybean (Glycine Max) flour and mint leaf (Mentha Piperita). Direct Research Journal of Agriculture and Food Science, 12(2), 274–278. https://doi.org/https://doi.org/10.26765/DRJAFS25926781
Wani, A.A., Sogi, D.S., Singh, P., Sharma, P., & Pangal, A. (2012). Dough-handling and cookie-making properties of wheat flour-watermelon protein isolate blends. Food and Bioprocess Technology, 5(5), 1612–21. https://doi.org/10.1007/s11947-010-0466-6
Wee, M.S.M., & Henry, C.J. (2020). Reducing the glycemic impact of carbohydrates on foods and meals: strategies for the food industry and consumers with special focus on Asia. Comprehensive Reviews in Food Science and Food Safety, 19(2), 670–702. https://doi.org/10.1111/1541-4337.12525
Yılmaz, V.A., & Koca, I. (2020). Development of gluten-free corn bread enriched with anchovy flour using TOPSIS multi-criteria decision method. International Journal of Gastronomy and Food Science, 22, 100281. https://doi.org/10.1016/j.ijgfs.2020.100281
Youssef, M. K. E., & Mousa, R.M.A. (2012). Nutritional assessment of wheat biscuits and fortified wheat biscuits with citrus peels powders. Food and Public Health, 2(1), 55–60. https://doi.org/10.5923/j.fph.20120201.11
Yudhistira, B., Sari, T.R., & Affandi, D.R. (2019). Karakteristik fisik, kimia dan organoleptik cookies bayam hijau (Amaranthus tricolor) dengan penambahan tomat (Solanum lycopersicum) sebagai upaya pemenuhan defisiensi zat besi pada anak-anak. Warta Industri Hasil Pertanian, 36(2), 83. https://doi.org/10.32765/wartaihp.v36i2.5286
Yuliantini, E., Kamsiah, K., & Meriwati, M. (2018). Biskuit “fishbean” sebagai alternatif MP-ASI lokal tinggi protein dan vitamin A. Jurnal Ilmu dan Teknologi Kesehatan, 6(1), 25–37.
Zebib, H.,Teame, T., Aregawi, T., & Meresa, T. (2020). Nutritional and sensory acceptability of wheat bread from fish flour. Cogent Food & Agriculture, 6(1), 1714831. https://doi.org/10.1080/23311932.2020.1714831
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