Karakteristik hidrolisat kolagen kulit ikan tenggiri kering dengan enzim papain The characteristics of spanish mackerel dry skin collagen hydrolisate with papain enzyme
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12 December 2024
23 December 2024
Abstract
Spanish mackerel dried skin is commonly used in chips, crackers, and empek-empek products. However, its potential for transformation into value-added products such as collagen hydrolysate still needs to be explored. Processing this material using papain enzyme offers an efficient method to enhance its economic value, owing to its high yield and effectiveness. The aim of this study was to determine the optimum concentration of papain enzyme and soaking time for the hydrolysis of dried mackerel skin collagen based on the characteristics of dried mackerel skin collagen. This study applied a completely randomized design (CRD) with two factors: papain enzyme concentration (1% and 2%) and soaking time (12 and 24 h). The results indicated that the interaction between enzyme concentration and soaking time significantly affected key parameters, including hydrolysis degree, whiteness, protein content, viscosity, gel strength, melting point, solubility, and foaming ability, whereas yield, moisture, ash, fat, pH, and zeta potential were unaffected. Optimal conditions were achieved with 2% papain enzyme and 24-hour soaking time, resulting in a 2.30% yield, 35.62% hydrolysis degree, 77.73% white degree, 6.94% moisture, 1.44% ash, 95.35% protein, 2.33% lipid, 6,13cp viscosity, 46.64 gBloom gel strength, 14.67OC melting point, 5.83 pH, 2.47mV zeta potential, 82.00% solubility, and 31.67% foaming ability. These results highlight the significant potential of Spanish mackerel dried skin as a raw material for collagen hydrolysate production, supporting its application in developing high-value fishery by-products and promoting sustainable economic development.
References
Afifah, A., Suparno, O., Haditjaroko, L., Tarman, K., Setiyono, A. & Nugraha, A. W. (2024). Isolation and characterization of collagen from salmon (Salmo salar) skin using papain enzyme. Jurnal Pengolahan Hasil Perikanan Indonesia, 27(6), 536-552. http://dx.doi.org/10.17844/jphpi.v27i6.53285.
Akter, S., Rahman, Md. A., Naher, J., Majumder, M. W. R. & Alam, AKM N. (2017). Fish glue from tilapia scale and skin and its physical and chemical characters. International Journal of Fisheries and Aquatic Studies, 5(2), 255-257.
Alfaro, A. d. T., Fonseca, G. G., Balbinot, E., Souza, N. E. d & Prentice, C. (2014). Yield, viscosity, and gel strength of wami tilapia (Oreochromis urolepis hornorum) skin gelatin: optimization of the extraction process. Food Science Biotechnology, 23(3), 765-773. https://doi.org/10.1007/s10068-014-0103-7.
Alhana, Suptijah, P. & Tarman, K. (2015). Ekstraksi dan karakterisasi kolagen dari daging teripang gamma. Jurnal Pengolahan Hasil Perikanan Indonesia, 18(2), 150-161. https://doi.org/10.17844/jphpi.v18i2.10610.
Almada, W. (2018). Profil peptida kolagen dalam hidrolisat kolagen sisik ikan gabus (Channa striata) hasil ekstraksi menggunakan enzim papain. [Skripsi]. Universitas Brawijaya.
Ambarwati, R. (2019). Ekstraksi dan karakterisasi papain soluble collagen dari kulit ikan patin. [Skripsi]. Universitas Gadjah Mada.
Amin, H. F., El-Lahamy, A. A., Mohamed, H. R., Khalil, K. I., Mahmud, A. A., Roby, M. H. H., El-Sherif, S. A. & Mohamed, A. S. (2023). Effect of frozen storage on fish quality and fishery products: A Review. Mediterranean Aquaculture Journal, 10(2), 25-35. https://doi.org/10.21608/maj.2024.334695.
Amri, E. & Mamboya, F. (2012). Papain, a plant enzyme of biological importance: A review. American Journal of Biochemistry and Biotechnology, 8(2), 99-104. https://doi.org/10.3844/ajbbsp.2012.99.104.
Anggraini, S. (2014). Pembuatan hidrolisat kolagen dari kulit dan tulang ikan patin (Pangasius pangasius) dengan enzim papain dan pengujian aktivitas antioksidannya. [Skripsi]. Universitas Sriwijaya.
Anggraini, A. & Yunianta. (2015). Pengaruh suhu dan lama hidrolisis enzim papain terhadap sifat kimia, fisik dan organoleptik sari edamame. Jurnal Pangan dan Agroindustri, 3(3), 1015-1025.
[AOAC] Association of Official Analytical of Chemists. (2005). Oficial methods of analysis (18 edn) association of official analytical of chemists, inc: arlington, Virginia.
Ariyanto, R., Ismawati & Hanafi, I. (2022, November 30). Pembuatan gelatin tulang ikan tengiri (Scomberomorus guttatus) dengan variasi penggunaan jenis cuka [Conference session]. Seminar Nasional Ekonomi dan Teknologi 2 Fakultas Pertanian Universitas Wiraraja Sumenep. https://doi.org/10.24929/prosd.v0i0.2378.
Asaduzzaman, A. K. M., Getachew, A. T., Cho, Y. J., Park J. S., Haq, M. & Chun, B. S. (2020). Characterization of pepsin-solubilised collagen recovered from mackerel (Scomber japonicus) bone and skin using subcritical water hydrolysis. International Journal of Biological Macromolecules, 149, 1290-1297. https://doi.org/10.1016/j.ijbiomac.2019.10.104.
Astiana, I. (2016). Efektivitas asam dan enzim papain dalam menghasilkan kolagen dari kulit ikan ekor kuning (Caesio cuning). [Tesis]. Institut Pertanian Bogor.
Azizah, N. (2019). Karakteristik gelatin kulit ikan patin (Pangasius hypopthalmus) dan hidrolisatnya sebagai antioksidan. [Skripsi]. Institut Pertanian Bogor.
Babu, K. M. (2013). Silk: Processing, properties and applications. Woodhead Publishing Limited, Ingram Publisher Services [distributor]. Sawston, LaVergne.
[BSN] Badan Standardisasi Nasional. (2014). SNI 8076-2014-Kolagen kasar dari sisik ikan. Badan Standardisasi Nasional. Jakarta.
Baehaki, A., Lestari, S. D. & Romadhoni, A. R. (2015). Hidrolisis protein ikan patin menggunakan enzim papain dan aktivitas antioksidan hidrolisatnya. Jurnal Pengolahan Hasil Perikanan Indonesia, 18(3), 230-239. https://doi.org/10.1016/10.17844/jphpi.2015.18.3.230.
Baehaki, A., Nopianti, R. & Anggraeni, S. (2015). Antioxidant activity of skin and bone collagen hydrolyzed from striped catfish (Pangasius pangasius) with papain enzyme. Journal of Chemical and Pharmaceutical Research, 7(11), 131-135.
Chen, P., Qiu, Y., Chen, S., Zhao, Y., Wu, Y. & Wang, Y. (2022). Insights into the effects of different drying methods on protein oxidation and degradation characteristics of golden pompano (Trachinotus ovatus). Front. Nutr. 9:1063836. https://doi.org/10.3389/fnut.2022.1063836.
Chi, C. F., Cao, Z. H., Wang, B., Hu, F. Y., Li, Z. R. & Zhang, B. (2014). Antioxidant and functional properties of collagen hydrolysates from spanish mackerel skin as influenced by average molecular weight. 19:11211-11230. https://doi.org/10.3390/molecules190811211.
Desliani, I. (2014). Aktivitas antioksidan hidrolisat kolagen kulit dan tulang ikan patin (Pangasius pangasius) yang dibuat dengan enzim bromelin. [Skripsi]. Universitas Sriwijaya.
Devi, H. L. N. A., Suptijah, P. & Nurilmala, M. (2017). Efektivitas alkali dan asam terhadap mutu kolagen dari kulit ikan patin. Jurnal Pengolahan Hasil Perikanan Indonesia, 20(2), 255-265.
Devita, L., Nurilmala, M., Lioe, H. N. & Suhartono, M. T. (2021). Chemical and antioxidant characteristics of skin-derived collagen obtained by acid-enzymatic hydrolysis of bigeye tuna (Thunnus obesus). Marine Drugs, 19(4), 222. https://doi.org/10.3390/md19040222.
Dwijayanti E., Munadi R. & Farnatubun M. W. (2023). Analisis proksimat dan kolagen pada kulit ikan tawassang (Naso thynnoides). ILTEK : Jurnal Teknologi, 18(2), 103-107. https://doi.org/10.47398/iltek.v18i02.124.
Dzyuba, N., Bilenka, I., Palvashova, A. & Zemlyakova, E. (2017). Investigation of foaming and hydration properties of collagen hydrolyzate. Eureka: Life Science no 5, 68-72. https://doi.org/10.21303/2504-5695.2017.00424.
Ennaas, N., Hammami, R., Gomaa, A., Bedard, F., Biron, E., Subirade, M., Beaulieu, L. & Fliss, I. (2016). Collagencin, an antibacterial peptide from fish collagen: activity, structure and interaction dynamics with membrane. Biochemical and Biophysical Research Communications, 473(2), 642-647. https://doi.org/10.1016/j.bbrc.2016.03.121.
Fawzya, Y. N., Chasanah, E, Poernomo, A. & Khirzin, M. H. (2016). Isolasi dan karakterisasi parsial kolagen dari teripang gamma (Stichopus variegatus). JPB Kelautan dan Perikanan, 11(1), 91-100.
Febriansyah, R., Pratama, A. & Gumilar, J. (2019). Pengaruh konsentrasi NaOH terhadap rendemen, kadar air dan kadar abu gelatin ceker itik (Anas platyrhynchos Javanica). Jurnal Ilmu dan Teknologi Hasil Ternak, 14(1), 1-10. https://doi.org/10.21776/ub.jitek.2019.014.01.1.
Fernianti, D., Juniar, H. & Adinda, N. D. (2020). Pengaruh massa ossein dan waktu ekstraksi gelatin dari tulang ikan tenggiri dengan perendaman asam sitrat belimbing wuluh. Jurnal Distilasi, 5(2), 1-9. https://doi.org/10.32502/jd.v5i2.3027.
Gadi, D. S., Trilaksani, W. & Nurhayati, T. (2017). Histologi, ekstraksi dan karakterisasi kolagen gelembung renang ikan cunang Muarenesox talabon. Jurnal Ilmu dan Teknologi Kelautan Tropis, 9(2), 665-683. http://dx.doi.org/10.29244/jitkt.v9i2.19300.
Gatea, A. A. (2011). Design and construction of a solar drying system, a cylindrical section and analysis of the performance of the thermal drying system. African Journal of Agricultural Research, 6(2), 343-351. https://doi.org/10.5897/AJAR10.347.
GMIA. (2012). Gelatin Handbook. USA: Gelatin Manufacturers Institute of America.
Gunawan, F., Suptijah, P. & Uju. (2017). Ekstraksi dan karakterisasi gelatin kulit ikan tenggiri (Scomberomorus commersonii) dari Provinsi Kepulauan Bangka Belitung. Jurnal Pengolahan Hasil Perikanan Indonesia, 20(3), 568-581. journal.ipb.ac.id/index.php/jphpi.
Hadfi, N. H. & Sarbon, N. M. (2019). Physicochemical properties of silver catfish (Pangasius sp.) skin collagen as influenced by acetic acid concentration. Food Research (2019): pp-pp.
Hanafiah, K. A. (2010). Rancangan percobaan teori & aplikasi ed 3. Raja Grafindo Persada. Banjarmasin.
Herawati, E., Titisari, R. S. & Astirin, O. P. (2023). Characterization of collagen hydrolysate gel from mackerel scad skin (Decapterus macarellus). Jurnal Penelitian Pendidikan IPA, 9(7), 5568-5573. https://doi.org/10.29303/jppipa.v9i7.3983.
Herson, N. A., Sumual, M. F., Rumengan, I. F. M., Pongoh, J. & Mandey, L. C. (2023). Analisis proksimat kolagen sisik ikan kakatua (Scarus sp). Jurnal Agroteknologi Terapan, 4(2), 428-433. https://doi.org/10.35791/jat.v4i2.52484.
Heu, M. S., Lee, J. H., Kim, H. J., Jee, S. J., Lee, J. S., Jeon, Y. J., Shahidi, F. & Kim, J. S. (2010). Characterization of acid and pepsin-soluble collagen from flatfish skin. Food Sci. Biotechnol, 19(1), 27-33. https://doi.org/10.1007/s10068-010-0004-3.
Khirzin, M. H., Sukarno, Yuliana N. D., Fawzya Y.N. & Chasanah E. (2015). Aktivitas inhibitor enzim pengubah angiotensin (ACE) dan antioksidan peptida kolagen dari teripang gamma (Stichopus variegatus). JPB Kelautan dan Perikanan, 10(1), 27-35. https://doi.org/10.15578/jpbkp.v10i1.242.
Kristinsson, H. G. & Rasco, B. A. (2000). Fish protein hydrolysates: production, biochemical, and functional properties. Critical Reviews in Food Science and Nutrition, 40(1), 43-81. https://doi. org/10.1080/10408690091189266.
Kusa, S. R., Naiu, A. S. & Yusuf, N. (2022). Karakteristik kolagen kulit tuna sirip kuning (Thunnus albacares) pada waktu hidro-ekstraksi berbeda dan potensinya dalam bentuk sediaan nanokolagen. Media Teknologi Hasil Perikanan, 10(2), 107-116. https://doi.org/10.35800/mthp.10.2.2022.41716.
Kusumaningrum, I. (2011). Optimasi ekstraksi dan karakterisasi gelatin kulit ikan tenggiri (Scomberromorus commersoni) segar dan kering [Tesis]. Universitas Gadjah Mada.
Kusumaningrum, I., Pranoto, Y. & Hadiwiyoto, S. (2018). Extraction optimization and characterization of gelatine from fish dry skin of Spanish mackerel (Scomberromorus commersoni). The 1st International Conference on Tropical Studies and Its Application (ICTROPS) 144 (2018) 012036. IOP Conference Series: Earth and Environmental Science. https://doi.org/10.1088/1755-1315/144/1/012036.
Lestari, S. I., Aina, G. Q. & Yusran D. I. (2022). Analisis pengaruh penyimpanan terhadap kadar protein pada ikan patin. Duta Pharma Journal, 2(2), 114-120. https://doi.org/10.47701/djp.v2i2.2434.
Maulid, D. Y., Putra, R. S., Nusaibah, Abrian, S., Pangestika, W., Arumsari, K., Widianto, D. I. & Yuniarti E. (2021). Kandungan proximat keripik kulit ikan tenggiri (Scomberomorus commerson) dengan metode pengeringan yang berbeda. AGRIKAN - Jurnal Agribisnis Perikanan, 14(2), 445-451. https://doi.org/10.52046/agrikan.v14i2.445-451.
Meilani, S. S., Kustiyah, E., Saing, B. & Ridwan, A. M. (2022). Pemanfaatan kembali limbah tulang ikan tenggiri sebagai bahan baku pembuatan gelatin melalui proses hidrolisis asam fosfat. Jurnal Ilmu Perikanan dan Kelautan, 4(2), 57-64. https://doi.org/10.36526/lemuru.v4i2.2085.
Munkhuu, B., Erdene, L., Bayarsukh, Z., Altantulga, E. A., Baltsukh, O., Tudev, G. E. & Narmandakh, A. (2021, October 05). Antioxidant and antihypertensive activity of collagen and elastin hydrolysate at different molecular weights [Conference session]. The 5th International Conference on Chemical Investigation and Utilization of Natural Resource (ICCIUNR-2021); Atlantis Highlights in Chemistry and Pharmaceutical Sciences Vol 2. https://doi.org/10.2991/ahcps.k.211004.004
Novitasari, A. E. (2021) Studi literatur pengaruh enzim protease (papain, bromelin dan protease biduri) terhadap kadar protein pada ikan laut menggunakan metode spektrofotometri dan kjedal. Jurnal Sains, 12(1), 1-19.
Nuraini, E. (2022). Karakteristik hidrolisat kolagen dari kulit ikan patin (Pangasius hypophthalmus) dengan perbedaan konsentrasi enzim papain. [Skripsi]. Institut Pertanian Bogor.
Nurilmala, M., Pertiwi, R. M., Nurhayati, T., Fauzi, S., Batubara, I. & Ochiai, Y. (2019). Characterization of collagen and its hydrolysate from yellowfin tuna Thunnus albacares skin and their potencies as antioxidant and antiglycation agents. Fisheries Science, 85(2019), 591-599. https://doi.org/10.1007/s12562-019-01303-5.
Nurjanah, Baharuddin, T. I. & Nurhayati, T. (2021). Ekstraksi kolagen kulit ikan tuna sirip kuning (Thunnus albacares) menggunakan enzim pepsin dan papain. Jurnal Pengolahan Hasil Perikanan Indonesia, 24(2), 174-187. http://dx.doi.org/10.17844/jphpi.v24i2.35410.
Ong, T. Y., Shaik, M. I. & Sarbon, N. M. (2021). Isolation and characterization of acid and pepsin soluble collagen extracted from sharpnose stingray (Dasyatis zugei) skin. Food Research, 5(3), 214-224. https://doi.org/10.26656/fr.2017.5(3).322.
Parvizi, J. & Kim, G. K. (2010). High Yield Orthopaedics. Chapter 53 - Collagen. Elsevier. https://doi.org/10.1016/B978-1-4160-0236-9.00064-X.
Pascacio, V. G. T., Valbuena, D. C., Sterling, R. M., Tavano, O., Murcia, A. B., Guti´errez, G. V., Rather, I. A. & Lafuente, R. F. (2021). Bioactive peptides from fisheries residues: A review of use of papain in proteolysis reactions. International Journal of Biological Macromolecules, 184(2021), 415-428. https://doi.org/10.1016/j.ijbiomac.2021.06.076.
Prasetyo, D. Y. B., Sarmin, Setyastuti, A. I. & Kurniawati, A. (2020). Pengaruh perbedaan enzim proteolitik dan lama hidrolisa terhadap kualitas hidrolisat protein ikan dari limbah industri fillet Ikan Nila (Oreochromisniloticus (Linnaeus, 1758)). Jurnal Ilmu Kelautan Kepulauan, 3(2), 202-210. https://doi.org/10.33387/jikk.v3i2.2586.
Prastyo, D. T., Trilaksani, W. & Nurjanah. (2020). Aktivitas antioksidan hidrolisat kolagen kulit ikan nila (Oreochromis niloticus). Jurnal Pengolahan Hasil Perikanan Indonesia, 23(3), 423-433.
Putra. A. B. N., Sahubawa, L. & Ekantari, N. (2013). Ekstraksi dan karakterisasi kolagen dari kulit ikan nila hitam (Oreochromis niloticus). Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan, 8(2), 171–180. http://dx.doi.org/10.15578/jpbkp.v8i2.61.
Putri, A. A. H. Q. (2022). Isolasi dan karakterisasi kolagen dari kulit dan tulang ikan tenggiri (Scomberomorus commerson). [Skripsi]. Universitas Islam Negeri Sunan Gunung Djati.
Rachmania, R. A., Nisma, F. & Mayangsari, E. (2013). Ekstraksi gelatin dari tulang ikan tenggiri melalui proses hidrolisis menggunakan larutan basa. Media Farmasi, 10(2), 18-28. http://dx.doi.org/10.12928/mf.v10i2.1167.
Rahmadayana, R. D. (2023). Analisis strategi pemasaran dan promosi melalui media online produk olahan kerupuk kulit ikan tenggiri ‘’Damai Jaya’’. JEK - Jurnal Ekonomi dan Kewirausahaan Kreatif, 8(1), 11-17. https://doi.org/10.59179/jek.v8i01.65.
Rahmawati, D. (2020). Pengaruh variasi jenis asam terhadap produksi kolagen berbahan dasar tulang ikan tongkol (Euthynnus affinis). [Skripsi]. UIN Maulana Malik Ibrahim.
Rahmawati, H. & Pranoto, Y. (2012). Sifat fisiko-kimia gelatin kulit ikan belut dan lele pada keadaan segar dan kering. Fishscientiae, 2(3), 18-30. http://dx.doi.org/10.20527/fs.v2i3.1148.
Rahmawati, S. & Nurjanah. (2020). Pengaruh konsentrasi enzim papain terhadap mutu gelatin bubuk dari tulang dan cakar ayam. Jurnal Konversi, 9(1), 39-52. https://doi.org/10.24853/konversi.9.1.14.
Ramli, A. R., Annisa, A. R., Bahmid, N. A. & Mustafa, M. D. (2020). Isolation of papain-soluble collagen from the skin of snake-head fish (Channa striata). Canrea Journal: Food Technology, Nutritions, and Culinary, 3(2), 87-93. https://doi.org/10.20956/canrea.v3i2.367.
Rasegaran, L. A. P. K., Jaziri, A. A., Aziz, A. H. A., Shapawi, R., Mokhtar, R. A. M., Noordin, W. N. Md., Nurdiani, R. & Huda N. (2024). Comparison of physicochemical properties of barracuda (Sphyraena barracuda Edwards, 1771) skin collagen hydrolyzed using two different pepsin sources. International Journal on Advanced Science Engineering Information Technology. 14(4), 1305-1311. https://doi.org/10.18517/ijaseit.14.4.19647.
Ratnasari, I. & Firlianty. (2016). Physico-chemical characterization and skin gelatin rheology of four freshwater fish as alternative gelatin source. AACL Bioflux, 9(6),1196-1207.
Raymundus, J. T., Mentang, F., Agustin, T. A., Onibala, H., Kaseger, B. E., Makapedua, D. M. & Sanger, G. (2020). Pengaruh perbedaan konsentrasi asam asetat dan lama waktu ekstraksi kolagen dari kulit ikan situhuk hitam (Makaira indica). Jurnal Media Teknologi Hasil Perikanan, 8 (2), 44-49. https://doi.org/10.35800/mthp.8.2.2020.27326.
Safira, A., Rani, C. A. M., Puspitasari, R. A., Ayuningtyas, A. K. P., Mahendra, Y. A., Purnomo, A., Fikri, F., Chhetri, S., Elziyad, M. T. & Purnama. (2022). Amino acid and proximate analysis of type-1 collagen from sea cucumber and tilapia-skin and its potential application as artificial tendon. Pharmacognosy Journal, 14(4):358-361. https://doi.org/10.5530/pj.2022.14.107.
Safithri, M., Setyaningsih, I., Tarman, K., Yuhendri, V. M. & Meydia. (2018). Potensi kolagen teripang emas sebagai inhibitor tirosinase. Jurnal Pengolahan Hasil Perikanan Indonesia, 21(2), 295-303. https://doi.org/10.17844/jphpi.v21i2.23085.
Safithri, M., Tarman, K., Suptijah, P. & Widowati, N. (2019). Karakteristik fisikokimia kolagen larut asam dari kulit ikan parang-parang (Chirocentrus dorab). Jurnal Pengolahan Hasil Perikanan Indonesia, 22(3), 441-452. journal.ipb.ac.id/index.php/jphpi.
Silvestre, M. P. C., Morais, H. A., Silva, V. D. M. & Silva, M. R. (2013). Degree of hydrolysis and peptide profile of whey proteins using pancreatin. Journal of the Brazilian Chemical Society, 38(1), 278-290. http://dx.doi.org/10.4322/nutrire.2013.026.
Suki, B. (2022). Structure and function of the extracellular matrix. A multiscale quantitative approach. Chapter 3 - The collagen molecule. Academic Press. https://doi.org/10.1016/B978-0-12-819716-5.00001-0.
Suptijah, P., Indriani, D. & Wardoyo, S. E. (2018). Isolasi dan karakterisasi kolagen dari kulit ikan patin (Pangasius sp.). Jurnal Sains Natural Universitas Nusa Bangsa, 8(1), 8-23. https://doi.org/10.31938/jsn.v8i1.106.
Surya, R., Destifen, W., Nugroho, D. & Stephanie. 2023. Pempek: traditional fishcake dish from South Sumatra, Indonesia. Canrea Journal: Food Technology, Nutritions, and Culinary, 6(1), 57-76. https://doi.org/10.20956/canrea.v6i1.964.
Tinrat, S. & and Sila-asna, M. (2017). Optimization of gelatin extraction and physico-chemical properties of fish skin and bone gelatin: its application to panna cotta formulas. Current Research in Nutrition and Food Science, 5(3), 263-273. http://dx.doi.org/10.12944/CRNFSJ.5.3.11.
Trilaksani, W., Nurilmala, M. & Setiawati, I. H. (2012). Ekstraksi gelatin kulit ikan kakap merah (Lutjanus sp.) dengan proses perlakuan asam. Jurnal Pengolahan Hasil Perikanan Indonesia, 15(3), 240-251. https://doi.org/10.17844/jphpi.v15i3.21436.
Tsurayya, E. A. (2014). Karakteristik gelatin kulit ikan tenggiri (Scomberromorus commersoni) dan aplikasinya untuk mikroenkapsulasi minyak atsiri daun salam (Pimenta racemosa) dengan metode freeze drying. [Skirpsi]. Universitas Gadjah Mada.
Wardani, D. W. (2022). Evaluasi in silico dan in vitro untuk aktivitas antioksidan dan sifat fungsional kolagen kulit ikan tuna sirip kuning (Thunnus albacares) yang dihidrolisis dengan enzim papain. [Tesis]. Universitas Gadjah Mada.
Wirayudha, R. H., Herawati, D., Kusnandar, F. & Nurhayati, T. (2022). Kapasitas antioksidan dan sifat fisikokimia hidrolisat kolagen dari kulit ikan tuna sirip kuning dengan metode ultrasound assisted enzymatic reaction. Jurnal Pengolahan Hasil Perikanan Indonesia, 25(3), 393-404. https://doi.org/10.17844/jphpi.v25i3.43325.
Wulandari, Suptijah P. & Tarman K. (2015). Efektivitas pretreatment alkali dan hidrolisis asam asetat terhadap karakteristik kolagen dari kulit ikan gabus. Jurnal Pengolahan Hasil Perikanan Indonesia, 18(3), 287-302. https://doi.org/10.17844/jphpi.2015.18.3.287.
Xuan Ky, P., Bao Vy, P., Ha Dao, V., Le Ho, K. H., Hong, N. T., Thiet, D. T. & Anh, N. P. (2018). Investigation of protein patterns and antioxidant activity of collagen hydrolysates from skin of fan-bellied leatherjacket Monacanthus chinensis by various enzymes. Journal of Marine Science and Technology, 18(4A), 141–150. DOI:10.15625/1859-3097/18/4A/13642.
Yanti, F., Dharmayanti, N. & Suryanti. (2022). Aktivitas antioksidan kolagen dari kulit ikan patin (Pangasius sp.) dengan enzim bromelin kasar kulit nanas (Ananas comosus L.). Jurnal Pengolahan Hasil Perikanan Indonesia, 25(1), 88-96. http://dx.doi.org/ 10.17844/jphpi.v25i1.36731.
Zeleny, M. (1982). Multiple criteria decision making 2nd Edition. Mc Graw Hill ISBN: 0070727953, 9780070727953. New York.
Zheng, Y., Li, Y., Ke, C., Gao, X., Liu, Z. & Chen, J. (2024). Comparison of structural and physicochemical characteristics of skin collagen from chum salmon (cold-water fish) and nile tilapia (warm-water fish). Foods 13(8), 1213. https://doi.org/10.3390/foods13081213.
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