Isolation and characterization of collagen from salmon (Salmo salar) skin using papain enzyme Isolasi dan karakterisasi kolagen dari kulit ikan salmon (Salmo salar) menggunakan enzim papain
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Accepted
16 May 2024
Published
5 June 2024
Keywords:
-
amino acid, byproduct, extraction, PaSC
Abstract
Kulit ikan salmon merupakan salah satu hasil samping yang dapat diolah menjadi kolagen. Kolagen larut papain (PaSC) adalah istilah yang digunakan untuk menggambarkan kolagen yang diekstraksi menggunakan kombinasi asam dan enzim papain. PaSC berpotensi menjadi sumber kolagen halal. Penelitian ini bertujuan untuk menentukan waktu ekstraksi dan konsentrasi enzim papain terbaik dalam menghasilkan kolagen kulit ikan salmon berdasarkan persentase rendemen dan sifat kimia. Kulit ikan salmon diekstrak menggunakan kombinasi asam asetat (0,5 M) dan enzim papain (500; 1.000; 1.500 U/mg/g kulit) selama 1; 2; dan 3 jam. Parameter yang dianalisis dalam penelitian ini meliputi logam berat, rendemen, asam amino, gugus fungsional, dan berat molekul. Hasil penelitian menunjukkan bahwa perlakuan kombinasi asam asetat konsentrasi 0,5 M dan enzim papain 1.000 U/mg/g selama 2 jam menghasilkan kelarutan kolagen yang maksimal dengan rendemen sebesar 15,38% (bk). Kolagen PaSC kulit ikan salmon terdeteksi memiliki asam amino prolin, glisin, dan arginin. Distribusi berat molekul kolagen berada pada rentang 20-142 kDa. Struktur triple helix kolagen tidak mengalami perubahan selama proses ekstraksi berdasarkan analisis FTIR.
References
Afifah, A., Suparno, O., Haditjaroko, L., & Tarman, K. (2019, September 20-21). Utilization of fish skin waste as a collagen wound dressing on burn injuries: A mini review [Conference session]. International Conference on Digital Agriculture from Land to Consumers, Bogor, Indonesia. IOP Conference Series: Earth and Environmental Science, 335(1). https://doi.org/10.1088/1755 1315/335/1/012031
Ali, A. M. M., Kishimura, H., & Benjakul, S. (2018). Extraction efficiency and characteristics of acid and pepsin soluble collagens from the skin of golden carp (Probarbus jullieni) as affected by ultrasonication. Process Biochemistry, 66(1), 237–244. https://doi.org/10.1016/j.procbio.2018.01.003
AOAC. (2019). Official methods of analysis of AOAC international 21st edition (21st ed.).
Aryee, A. N. A., Simpson, B. K., Phillip, L. E., & Cue, R. I. (2012). Effect of temperature and time on the stability of salmon skin oil during storage. JAOCS, Journal of the American Oil Chemists’ Society, 89(2), 287–292. https://doi.org/10.1007/s11746-011-1902-0
Astiana, I., Nurjanah, N., & Nurhayati, T. (2016). Characterization of acid soluble collagen from redbelly yellowtail fusilier fish skin (Caesio cuning). Jurnal Pengolahan Hasil Perikanan Indonesia, 19(1), 79–93. https://doi.org/10.17844/jphpi.2016.19.1.79
Badan Standarisasi Nasional. (1992). SNI 01- 2891-1992. Cara uji makanan dan miniuman. Serat kasar.
Badan Standarisasi Nasional. (2006a). SNI 01-2354.5-2006. Cara uji kimia -Bagian 5: Penentuan kadar logam berat kadmium (Cd) pada produk perikanan.
Badan Standarisasi Nasional. (2006b). SNI 01-2354.7 – 2006. Cara uji kimia -Bagian 7: Penentuan kadar logam berat timbal (Pb) pada produk perikanan.
Badan Standarisasi Nasional. (2006c). SNI 01-2354.1-2006. Cara uji kimia - bagian 1: penentuan kadar abu dan abu tidak larut asam pada produk perikanan.
Badan Standarisasi Nasional. (2006d). SNI 01-2354.2-2006. Cara uji kimia – Bagian 2: Pengujian kadar air pada produk perikanan.
Badan Standarisasi Nasional. (2006e). SNI 01-2354.3-2006. Cara Uji Kimia-Bagian 3: Penentuan Kadar Lemak Total pada Produk Perikanan.
Badan Standarisasi Nasional. (2006f). SNI 01-2354.4-2006. Chemical Test Method - Part 4: Determination of Protein Content by Total Nitrogen Method in Fishery Products.
Badan Standarisasi Nasional. (2016). SNI 01-2354.6-2016. Cara uji kimia -Bagian 6: Penentuan kadar logam berat merkuri (Hg) pada produk perikanan.
Badan Standarisasi Nasional. (1998). SNI 01-4866-1998. Cara uji cemaran arsen dalam makanan.
Bhagwat, P., & Dandge, P. (2016). Isolation, characterization and valorizable applications of fish scale collagen in food and agriculture industries. Biocatalysis and Agricultural Biotechnology, 7(9), 234–240. https://doi.org/10.1016/j.bcab.2016.06.010
Boronat, Ò., Sintes, P., Celis, F., Díez, M., Ortiz, J., Aguiló-Aguayo, I., & Martin-Gómez, H. (2023). Development of added-value culinary ingredients from fish waste: Fish bones and fish scales. International Journal of Gastronomy and Food Science, 31(December 2022), 0–6. https://doi.org/10.1016/j.ijgfs.2022.100657
Cahyono, H., Trilaksani, W., & Uju. (2022). Karakteristik fisikokimia papain soluble collagen dari gelembung renang ikan tuna (Thunnus sp). Jurnal Pengolahan Hasil Perikanan Indonesia, 25(1), 1-17.https://doi.org/10.17844/jphpi.v25i1.38213
Cardoso, V., Quelemes, P., Amorin, A., Primo, F., Gobo, G., Tedesco, A., Mafud, A., Mascarenhas, Y., Corrêa, J., Kuckelhaus, S., Eiras, C., Leite, J., Silva, D., & Junior, J. (2014). Collagen based silver nanoparticles for biological applications: synthesis and characterization. Journal of Nanobiotechnology, 12(36), 1–9. 10.1186/s12951-014-0036-6
Chi, C., Cao, Z., Wang, B., Hu, F., Li, Z., & Zhang, B. (2014). Antioxidant and functional properties of collagen hydrolysates from spanish mackerel skin as influenced by average molecular weight. Molecules, 19(8), 11211–11230. https://doi.org/10.3390/molecules190811211
Cui, F., Xue, C., Li, Z., & Zhang, Y. (2007). Characterization and subunit composition of collagen from the body wall of sea cucumber Stichopus japonicus. Food Chemistry, 100(3), 1120–1125. https://doi.org/10.1016/j.foodchem.2005.11.019
Doyle, B. B., Bendit, E. G., & Blout, E. R. (1975). Infrared spectroscopy of collagen and collagen‐like polypeptides. Biopolymers, 14(5), 937–957. https://doi.org/10.1002/bip.1975.360140505
Fontaine-Vive, F., Merzel, F., Johnson, M. R., & Kearley, G. J. (2009). Collagen and component polypeptides: Low frequency and amide vibrations. Chemical Physics, 355(2–3), 141–148. https://doi.org/10.1016/j.chemphys.2008.12.005
Friess, W. (1998). Collagen-biomaterial for drug delivery. European Journal of Pharmaceutics and Biopharmaceutics, 45(2), 113–136. https://doi.org/10.1016/S0939-6411(98)00017-4
Gelse, K., Pöschl, E., & Aigner, T. (2003). Collagens-structure, function, and biosynthesis. Advanced Drug Delivery Reviews, 55(12), 1531–1546. https://doi.org/10.1016/j.addr.2003.08.002
Ghomi, E. R, Nourbakhsh, N., Akbari Kenari, M., Zare, M., & Ramakrishna, S. (2021). Collagen-based biomaterials for biomedical applications. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 109(12), 1986–1999. https://doi.org/10.1002/jbm.b.34881
Gómez-Guillén, M. C., & Montero, P. (2001). Extraction of gelatin from megrim (Lepidorhombus boscii) skins with several organic acids. Food Chemistry and Toxicology, 66(2), 213–216. https://doi.org/10.1111/j.1365-2621.2001.tb11319.x
Grzonka, Z., Kasprzykowski, F., & Wiczk, W. (2007). Cystein Proteases. In J. Polaina, A. P. MacCabe (eds), Industrial Enzymes (pp. 181–195). Springer, Dordrecht. https://doi.org/10.1007/1-4020-5377-0_11
Hardiyanti, R. (2017). Optimization of extraction and characterization of collagen from the skin of white snapper (Lates Calcarifer). [Skripsi]. Institut Pertanian Bogor.
Hashim, P., Ridzwan, M. S. M., & Bakar, J. (2014). Isolation and characterization of collagen from chicken feet. International Journal of Bioengineering and Life Sciences, 8(3), 250–254. doi.org/10.5281/zenodo.1091484
Hema, G., Shyni, K., Mthew, S., Ananda, R., Ninan, G., & Lakshmanan, P. (2013). A simple method for isolation of fish skin collagen-biochemical characterization of skin collagen extracted from albacore tuna (Thunnus alalunga), dog shark (Scoliodon sorrakowah), and Rohu (Labeo rohita). Annals of Biological Research, 4(1), 271–278.
Huang, C., Kuo, J., Wu, S., & Tsai, H. (2016). Isolation and characterization of fish scale collagen from tilapia (Oreochromis sp.) by a novel extrusion–hydroextraction process. Food Chemistry, 190(1), 997–1006. https://doi.org/10.1016/j.foodchem.2015.06.066
Huang, Y. R., Shiau, C. Y., Chen, H. H., & Huang, B. C. (2011). Isolation and characterization of acid and pepsin-solubilized collagens from the skin of balloon fish (Diodon holocanthus). Food Hydrocolloids, 25(6), 1507–1513. https://doi.org/10.1016/j.foodhyd.2011.02.011
Jafari, H., Lista, A., Siekapen, M. M., Ghaffari-Bohlouli, P., Nie, L., Alimoradi, H., & Shavandi, A. (2020). Fish collagen: extraction, characterization, and applications for biomaterials engineering. Polymers, 12(10), 1–37. https://doi.org/10.3390/polym12102230
Jamilah, B., Razali, U. M. R., Hashim, D, M., & Sazili, A. Q. (2013). Properties of collagen from barramundi (Lates calcarifer) skin. International Food Research Journal, 20(2), 791–798.
Joeng, H., Venkatesan, J., & Kim, S. (2013). Isolation and characterization of collagen from marine fish (Thunnus obesus). Biotechnol Bioprocess Eng, 18(6), 1185–1191.
Jongjareonrak, A., Benjakul, S., Visessanguan, W., Nagai, T., & Tanaka, M. (2005). Isolation and characterisation of acid and pepsin-solubilised collagens from the skin of Brownstripe red snapper (Lutjanus vitta). Food Chemistry, 93(3), 475–484. https://doi.org/10.1016/j.foodchem.2004.10.026
Katili, A. S. (2009). Struktur Dan Fungsi Protein Kolagen. Jurnal Pelangi Ilmu, 2(5), 19-29.
Kittiphattanabawon, P., Benjakul, S., Sinthusamran, S., & Kishimura, H. (2015). Characteristics of collagen from the skin of clown featherback (Chitala ornata). International Journal of Food Science and Technology, 50(9), 1972–1978. https://doi.org/10.1111/ijfs.12864
Kittiphattanabawon, P., Benjakul, S., Visessanguan, W., Kishimura, H., & Shahidi, F. (2010). Isolation and characterisation of collagen from the skin of brownbanded bamboo shark (Chiloscyllium punctatum). Food Chemistry, 119(4), 1519–1526. https://doi.org/10.1016/j.foodchem.2009.09.037
Kittiphattanabawon, P., Benjakul, S., Visessanguan, W., Nagai, T., & Tanaka, M. (2005). Characterisation of acid-soluble collagen from skin and bone of bigeye snapper (Priacanthus tayenus). Food Chemistry, 89(3), 363–372. https://doi.org/10.1016/j.foodchem.2004.02.042
Kittiphattanabawon, P., Sriket, C., Kishimura, H., & Benjakul, S. (2019). Characteristics of acid and pepsin solubilized collagens from nile tilapia (Oreochromis niloticus) scale. Emirates Journal of Food and Agriculture, 31(2), 95–101. https://doi.org/10.9755/ejfa.2019.v31.i2.1911
Kong, J., & Yu, S. (2007). Fourier transform infrared spectroscopic analysis of protein secondary structures. Acta Biochimimica et Biophysica Sinica, 39(8), 549–559. https://doi.org/10.1111/j.1745-7270.2007.00320.x
Kryndushkin, D. S., Alexandrov, I. M., Ter-avanesyan, M. D., Vitaly, V., Chem, J. B., Kryndushkin, D. S., Alexandrov, I. M., Ter-avanesyan, M. D., & Kushnirov, V. V. (2003). Protein structure and folding : Yeast [ PSI + ] prion aggregates are formed by small sup35 polymers fragmented by hsp104. The Journal of Biological Chemistry, 278(49), 49636–49643. https://doi.org/10.1074/jbc.M307996200
Kumar, N. S., & Nazeer, R. A. (2013). Characterization of acid and pepsin soluble collagen from the skin of horse mackerels (Magalaspis cordyla) and croaker (Otolithes ruber). International Journal of Food Properties, 16(3), 613–621. https://doi.org/10.1080/10942912.2011.557796
Laemmli. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature Publishing Group, 227(5259), 680–685. http://www.mendeley.com/research/discreteness-conductance-chnge-n-bimolecular-lipid-membrane-presence-certin-antibiotics/
Lestari, V., Kusumaningrum, I., Zuraida, I., Diachanty, S., & Pamungkas, B. F. (2024). Pemanfaatan kepala dan tulang ikan bawis (Siganus canaliculatus) pada pengolahan kerupuk. Jurnal Pengolahan Hasil Perikanan Indonesia, 27(1), 16-26. http://dx.doi.org/10.17844/jphpi.v27i1.45014
Liu, D., Liang, L., Regenstein, J., & Zhou, P. (2012). Extraction and characterisation of pepsin-solubilised collagen from fins, scales, skins, bones and swim bladders of bighead carp (Hypophthalmichthys nobilis). Food Chem, 133(4), 1441–1448.
Mahboob, S. (2015). Isolation and characterization of collagen from fish waste material- skin, scales and fins of Catla catla and Cirrhinus mrigala. Journal of Food Science and Technology, 52(7), 4296–4305. https://doi.org/10.1007/s13197-014-1520-6
Matinong, A. M. E., Chisti, Y., Pickering, K. L., & Haverkamp, R. G. (2022). Collagen Extraction from Animal Skin. Biology, 11(6), 1–15. https://doi.org/10.3390/biology11060905
Matmaroh, K., Benjakul, S., Prodpran, T., Encarnacion, A., & Kishimura, H. (2011). Characteristics of acid soluble collagen and pepsin soluble collagen from scale of spotted golden goatfish (Parupeneus heptacanthus). Food Chemistry, 129(3), 1179–1186. https://doi.org/10.1016/j.foodchem.2011.05.099
Muyonga, J. H., Cole, C. G. B., & Duodu, K. G. (2004). Characterisation of acid soluble collagen from skins of young and adult nile perch (Lates niloticus). Food Chemistry, 85(1), 81–89. https://doi.org/10.1016/j.foodchem.2003.06.006
Nagai, T., & Suzuki, N. (2000). Isolation of collagen from fish waste material – skin, bone, and fins.Food Chemistry, 68(3), 277-281. https://doi.org/10.1016/S0308-8146(99)00188-0
Nagarajan, M., Benjakul, S., Prodpran, T., & Songtipya, P. (2012). Food hydrocolloids characteristics and functional properties of gelatin from splendid squid (Loligo formosana) skin as affected by extraction temperatures. Food Hydrocolloids, 29(2), 389–397. https://doi.org/10.1016/j.foodhyd.2012.04.001
Nalinanon, S., Benjakul, S., Kishimura, H., & Osako, K. (2011). Type I collagen from the skin of ornate threadfin bream (Nemipterus hexodon): characteristics and effect of pepsin hydrolysis. Food Chemistry, 125(2), 500–507.
Nollet, L. M. L. (2004). Handbook of Food Analysis Volume 1: Physical Characterization and Nutrient Analysis. In Public Health (Vol. 39). https://doi.org/10.1016/S0033-3506(25)80037-4
Nurhayati, T., Nurjanah., & Astiana, I. (2018, November 6-8). Characteristics of papain soluble collagen from redbelly yellowtail fusilier (Caesio cuning) [Conference session]. Sustainable Agriculture Transformation for The Nations Welfare of Indonesia and Malaysia, Serdang, Selangor, Malaysia. IOP Conference Series: earth and environmental science, 196012034.
Nurjanah., Baharuddin, T. I., & Nurhayati, T. (2021). Ekstraksi kolagen kulit ikan tuna sirip kuning (Thunnus albacares) menggunakan enzim pepsin dan papain. Jurnal Pengolahan Hasil Perikanan Indonesia, 24(2), 174-187. https://doi.org/10.17844/jphpi.v24i2.35410
Prastyo, D. T., Trilaksani, W., & Nurjanah. (2020). Aktivitas antioksidan hidrolisat kolagen kulit ikan nila (Oreochromis niloticus). Jurnal Pengolahan Hasil Perikanan Indonesia, 23(3), 423-433. https://doi.org/10.17844/jphpi.v23i3.31732
Ramli, A. R., Annisa, A. R., Bahmid, N. A., & Mustafa, M. D. (2020). Isolation of papain-soluble collagen from the skin of snake-head fish (Channa striata). Canrea Journal: Food Technology, Nutritions, and Culinary Journal, 3(2), 87–93. https://doi.org/10.20956/canrea.v3i2.367
Regenstein, J. M., & Zhou, P. (2006). Collagen and gelatin from marine by-products. In maximising the value of marine by-products. Woodhead Publishing Limited. https://doi.org/10.1533/9781845692087.2.279
Saito, M., Kunisaki, N., Urano, N., &Kimura, S. (2002). Collagen as the major edible component of sea cucumber (Stichopus japonicus). Food Chemistry and Toxicology, 67(4), 1319–1322. https://doi.org/10.1111/j.1365-2621.2002.tb10281.x
Schmidt, M. M., Dornelles, R. C. P., Mello, R. O., Kubota, E. H., Mazutti, M. A., Kempka, A. P., & Demiate, I. M. (2016). Collagen extraction process. International Food Research Journal, 23(3), 913–922.
Silvipriya, K., Kumar, K., Bhat, A., Kumar, B., John, A., & Lakshmanan, P. (2015). Collagen: animal sources and biomedical application. Journal of Applied Pharmarmaceutical Science, 5(3), 123–127. https://doi.org/10.7324/JAPS.2015.50322
Singh, P., Benjakul, S., Maqsood, S., & Kishimura, H. (2011). Isolation and characterisation of collagen extracted from the skin of striped catfish (Pangasianodon hypophthalmus). Food Chemistry, 124(1), 97–105. https://doi.org/10.1016/j.foodchem.2010.05.111
Subhan, F., Hussain, Z., Tauseef, I., Shehzad, A., & Wahid, F. (2021). A review on recent advances and applications of fish collagen. Critical Reviews in Food Science and Nutrition, 61(6), 1027–1037. https://doi.org/10.1080/10408398.2020.1751585
Sumandiarsa, I. K., Siregar, R. R., Ayu, K., & Dewi, S. (2020). Pengaruh metode pemasakan terhadap nilai sensori dan profil asam amino cakalang (Katsuwonus pelamis) masak. Jurnal Kelautan dan Perikanan Terapan, 3(2), 51–57. https://doi.org/10.15578/jkpt.v3i2.8719
Sumarlin, L. O., Nurbayti, S., & Fauziah, S. (2012). Penghambatan enzim pemecah protein (enzim papain) oleh ekstrak rokok, minuman beralkohol dan kopi secara in vitro. Jurnal Kimia Valensi, 2(3), 449 - 458. https://doi.org/10.15408/jkv.v2i3.116
Suseno, H. S., Pari, R. F., Ibrahim, B., Ramadhan, R. T. K., Listiana, D., Nurjannah, F., & Adha, A. S. A. (2023). Profil asam lemak minyak dari jeroan ikan nila dan mas dengan rasio pelarut yang berbeda. Jurnal Pengolahan Hasil Perikanan Indonesia, 26(3), 460-475. http://dx.doi.org/10.17844/jphpi.v26i3.45781
Suseno, S. H., Riskon, A. K., Jacoeb, A. M., Nurjanah, & Supinah, P. (2020). Ekstraksi dry rendering dan karakterisasi minyak ikan patin (Pangasius sp.) hasil samping industri filet di Lampung. Jurnal Pengolahan Hasil Perikanan Indonesia, 23(1), 38-46. https://doi.org/10.17844/jphpi.v23i1.30722
Tamilmozhi, S., Veeruraj, A., & Arumugam, M. (2013). Isolation and characterization of acid and pepsin-solubilized collagen from the skin of sailfish (Istiophorus platypterus). Food Research International, 54(2), 1499–1505. https://doi.org/10.1016/j.foodres.2013.10.002
Terzi, A., Gallo, N., Bettini, S., Sibillano, T., Altamura, D., Madaghiele, M., De Caro, L., Valli, L., Salvatore, L., Sannino, A., & Giannini, C. (2020). Sub- and supramolecular X-Ray characterization of engineered tissues from equine tendon, bovine dermis, and fish skin type-I collagen. Macromolecular Bioscience, 20(5), 1–18. https://doi.org/10.1002/mabi.202000017
Tylingo, R., & Mania, S. (2016). Isolation and characterization of acid soluble collagen from the skin of African catfish (Clarias gariepinus), salmon (Salmo salar) and baltic cod (Gadus morhua). Journal of Biotechnology & Biomaterials, 6(2), 1-6. https://doi.org/10.4172/2155-952x.1000234
Waters. (2012). Acquity UPLC H class and H class bio amino acid analysis system guide. Waters Corporation. https://www.waters.com/webassets/cms/support/docs/acq_uplc_h-class_aaa_sysgd_rev_b.pdf
Widiyanto., Uju., & Nurilmala, M. (2022). Karakteristik kolagen dari kulit dan sisik ikan coklatan, swanggi, dan kurisi sebagai bahan gelatin. Jurnal Pengolahan Hasil Perikanan Indonesia, 25(3), 512-527. http://dx.doi.org/10.17844/jphpi.v25i3.43598
Woo, J. W., Yu, S. J., Cho, S. M., Lee, Y. B., & Kim, S. B. (2008). Extraction optimization and properties of collagen from yellowfin tuna (Thunnus albacares) dorsal skin. Food Hydrocolloids, 22(5), 879–887. https://doi.org/10.1016/j.foodhyd.2007.04.015
Yanti, F., Dharmayanti, N., & Suryanti. (2022). Aktivitas antioksidan kolagen dari kulit ikan patin (Pangasius sp.) dengan enzim bromelin kasar kulit nanas (Ananas comosus L.). Jurnal Pengolahan Hasil Perikanan Indonesia, 25(1), 88-96. https://doi.org/10.17844/jphpi.v25i1.36731
Zamorano-Apodaca, J. C., García-Sifuentes, C. O., Carvajal-Millán, E., Vallejo-Galland, B., Scheuren-Acevedo, S. M., & Lugo-Sánchez, M. E. (2020). Biological and functional properties of peptide fractions obtained from collagen hydrolysate derived from mixed by-products of different fish species. Food Chemistry, 331(10), 127350. https://doi.org/10.1016/j.foodchem.2020.127350
Zeng, S. kui, Zhang, C. hua, Lin, H., Yang, P., Hong, P. zhi, & Jiang, Z. (2009). Isolation and characterisation of acid-solubilised collagen from the skin of nile tilapia (Oreochromis niloticus). Food Chemistry, 116(4), 879–883. https://doi.org/10.1016/j.foodchem.2009.03.038
Zhang, F., Wang, A., Li, Z., He, S., & Shao, L. (2011). Preparation and characterisation of collagen from freshwater fish scales. Food and Nutrition Sciences, 02(08), 818–823. https://doi.org/10.4236/fns.2011.28112
Ali, A. M. M., Kishimura, H., & Benjakul, S. (2018). Extraction efficiency and characteristics of acid and pepsin soluble collagens from the skin of golden carp (Probarbus jullieni) as affected by ultrasonication. Process Biochemistry, 66(1), 237–244. https://doi.org/10.1016/j.procbio.2018.01.003
AOAC. (2019). Official methods of analysis of AOAC international 21st edition (21st ed.).
Aryee, A. N. A., Simpson, B. K., Phillip, L. E., & Cue, R. I. (2012). Effect of temperature and time on the stability of salmon skin oil during storage. JAOCS, Journal of the American Oil Chemists’ Society, 89(2), 287–292. https://doi.org/10.1007/s11746-011-1902-0
Astiana, I., Nurjanah, N., & Nurhayati, T. (2016). Characterization of acid soluble collagen from redbelly yellowtail fusilier fish skin (Caesio cuning). Jurnal Pengolahan Hasil Perikanan Indonesia, 19(1), 79–93. https://doi.org/10.17844/jphpi.2016.19.1.79
Badan Standarisasi Nasional. (1992). SNI 01- 2891-1992. Cara uji makanan dan miniuman. Serat kasar.
Badan Standarisasi Nasional. (2006a). SNI 01-2354.5-2006. Cara uji kimia -Bagian 5: Penentuan kadar logam berat kadmium (Cd) pada produk perikanan.
Badan Standarisasi Nasional. (2006b). SNI 01-2354.7 – 2006. Cara uji kimia -Bagian 7: Penentuan kadar logam berat timbal (Pb) pada produk perikanan.
Badan Standarisasi Nasional. (2006c). SNI 01-2354.1-2006. Cara uji kimia - bagian 1: penentuan kadar abu dan abu tidak larut asam pada produk perikanan.
Badan Standarisasi Nasional. (2006d). SNI 01-2354.2-2006. Cara uji kimia – Bagian 2: Pengujian kadar air pada produk perikanan.
Badan Standarisasi Nasional. (2006e). SNI 01-2354.3-2006. Cara Uji Kimia-Bagian 3: Penentuan Kadar Lemak Total pada Produk Perikanan.
Badan Standarisasi Nasional. (2006f). SNI 01-2354.4-2006. Chemical Test Method - Part 4: Determination of Protein Content by Total Nitrogen Method in Fishery Products.
Badan Standarisasi Nasional. (2016). SNI 01-2354.6-2016. Cara uji kimia -Bagian 6: Penentuan kadar logam berat merkuri (Hg) pada produk perikanan.
Badan Standarisasi Nasional. (1998). SNI 01-4866-1998. Cara uji cemaran arsen dalam makanan.
Bhagwat, P., & Dandge, P. (2016). Isolation, characterization and valorizable applications of fish scale collagen in food and agriculture industries. Biocatalysis and Agricultural Biotechnology, 7(9), 234–240. https://doi.org/10.1016/j.bcab.2016.06.010
Boronat, Ò., Sintes, P., Celis, F., Díez, M., Ortiz, J., Aguiló-Aguayo, I., & Martin-Gómez, H. (2023). Development of added-value culinary ingredients from fish waste: Fish bones and fish scales. International Journal of Gastronomy and Food Science, 31(December 2022), 0–6. https://doi.org/10.1016/j.ijgfs.2022.100657
Cahyono, H., Trilaksani, W., & Uju. (2022). Karakteristik fisikokimia papain soluble collagen dari gelembung renang ikan tuna (Thunnus sp). Jurnal Pengolahan Hasil Perikanan Indonesia, 25(1), 1-17.https://doi.org/10.17844/jphpi.v25i1.38213
Cardoso, V., Quelemes, P., Amorin, A., Primo, F., Gobo, G., Tedesco, A., Mafud, A., Mascarenhas, Y., Corrêa, J., Kuckelhaus, S., Eiras, C., Leite, J., Silva, D., & Junior, J. (2014). Collagen based silver nanoparticles for biological applications: synthesis and characterization. Journal of Nanobiotechnology, 12(36), 1–9. 10.1186/s12951-014-0036-6
Chi, C., Cao, Z., Wang, B., Hu, F., Li, Z., & Zhang, B. (2014). Antioxidant and functional properties of collagen hydrolysates from spanish mackerel skin as influenced by average molecular weight. Molecules, 19(8), 11211–11230. https://doi.org/10.3390/molecules190811211
Cui, F., Xue, C., Li, Z., & Zhang, Y. (2007). Characterization and subunit composition of collagen from the body wall of sea cucumber Stichopus japonicus. Food Chemistry, 100(3), 1120–1125. https://doi.org/10.1016/j.foodchem.2005.11.019
Doyle, B. B., Bendit, E. G., & Blout, E. R. (1975). Infrared spectroscopy of collagen and collagen‐like polypeptides. Biopolymers, 14(5), 937–957. https://doi.org/10.1002/bip.1975.360140505
Fontaine-Vive, F., Merzel, F., Johnson, M. R., & Kearley, G. J. (2009). Collagen and component polypeptides: Low frequency and amide vibrations. Chemical Physics, 355(2–3), 141–148. https://doi.org/10.1016/j.chemphys.2008.12.005
Friess, W. (1998). Collagen-biomaterial for drug delivery. European Journal of Pharmaceutics and Biopharmaceutics, 45(2), 113–136. https://doi.org/10.1016/S0939-6411(98)00017-4
Gelse, K., Pöschl, E., & Aigner, T. (2003). Collagens-structure, function, and biosynthesis. Advanced Drug Delivery Reviews, 55(12), 1531–1546. https://doi.org/10.1016/j.addr.2003.08.002
Ghomi, E. R, Nourbakhsh, N., Akbari Kenari, M., Zare, M., & Ramakrishna, S. (2021). Collagen-based biomaterials for biomedical applications. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 109(12), 1986–1999. https://doi.org/10.1002/jbm.b.34881
Gómez-Guillén, M. C., & Montero, P. (2001). Extraction of gelatin from megrim (Lepidorhombus boscii) skins with several organic acids. Food Chemistry and Toxicology, 66(2), 213–216. https://doi.org/10.1111/j.1365-2621.2001.tb11319.x
Grzonka, Z., Kasprzykowski, F., & Wiczk, W. (2007). Cystein Proteases. In J. Polaina, A. P. MacCabe (eds), Industrial Enzymes (pp. 181–195). Springer, Dordrecht. https://doi.org/10.1007/1-4020-5377-0_11
Hardiyanti, R. (2017). Optimization of extraction and characterization of collagen from the skin of white snapper (Lates Calcarifer). [Skripsi]. Institut Pertanian Bogor.
Hashim, P., Ridzwan, M. S. M., & Bakar, J. (2014). Isolation and characterization of collagen from chicken feet. International Journal of Bioengineering and Life Sciences, 8(3), 250–254. doi.org/10.5281/zenodo.1091484
Hema, G., Shyni, K., Mthew, S., Ananda, R., Ninan, G., & Lakshmanan, P. (2013). A simple method for isolation of fish skin collagen-biochemical characterization of skin collagen extracted from albacore tuna (Thunnus alalunga), dog shark (Scoliodon sorrakowah), and Rohu (Labeo rohita). Annals of Biological Research, 4(1), 271–278.
Huang, C., Kuo, J., Wu, S., & Tsai, H. (2016). Isolation and characterization of fish scale collagen from tilapia (Oreochromis sp.) by a novel extrusion–hydroextraction process. Food Chemistry, 190(1), 997–1006. https://doi.org/10.1016/j.foodchem.2015.06.066
Huang, Y. R., Shiau, C. Y., Chen, H. H., & Huang, B. C. (2011). Isolation and characterization of acid and pepsin-solubilized collagens from the skin of balloon fish (Diodon holocanthus). Food Hydrocolloids, 25(6), 1507–1513. https://doi.org/10.1016/j.foodhyd.2011.02.011
Jafari, H., Lista, A., Siekapen, M. M., Ghaffari-Bohlouli, P., Nie, L., Alimoradi, H., & Shavandi, A. (2020). Fish collagen: extraction, characterization, and applications for biomaterials engineering. Polymers, 12(10), 1–37. https://doi.org/10.3390/polym12102230
Jamilah, B., Razali, U. M. R., Hashim, D, M., & Sazili, A. Q. (2013). Properties of collagen from barramundi (Lates calcarifer) skin. International Food Research Journal, 20(2), 791–798.
Joeng, H., Venkatesan, J., & Kim, S. (2013). Isolation and characterization of collagen from marine fish (Thunnus obesus). Biotechnol Bioprocess Eng, 18(6), 1185–1191.
Jongjareonrak, A., Benjakul, S., Visessanguan, W., Nagai, T., & Tanaka, M. (2005). Isolation and characterisation of acid and pepsin-solubilised collagens from the skin of Brownstripe red snapper (Lutjanus vitta). Food Chemistry, 93(3), 475–484. https://doi.org/10.1016/j.foodchem.2004.10.026
Katili, A. S. (2009). Struktur Dan Fungsi Protein Kolagen. Jurnal Pelangi Ilmu, 2(5), 19-29.
Kittiphattanabawon, P., Benjakul, S., Sinthusamran, S., & Kishimura, H. (2015). Characteristics of collagen from the skin of clown featherback (Chitala ornata). International Journal of Food Science and Technology, 50(9), 1972–1978. https://doi.org/10.1111/ijfs.12864
Kittiphattanabawon, P., Benjakul, S., Visessanguan, W., Kishimura, H., & Shahidi, F. (2010). Isolation and characterisation of collagen from the skin of brownbanded bamboo shark (Chiloscyllium punctatum). Food Chemistry, 119(4), 1519–1526. https://doi.org/10.1016/j.foodchem.2009.09.037
Kittiphattanabawon, P., Benjakul, S., Visessanguan, W., Nagai, T., & Tanaka, M. (2005). Characterisation of acid-soluble collagen from skin and bone of bigeye snapper (Priacanthus tayenus). Food Chemistry, 89(3), 363–372. https://doi.org/10.1016/j.foodchem.2004.02.042
Kittiphattanabawon, P., Sriket, C., Kishimura, H., & Benjakul, S. (2019). Characteristics of acid and pepsin solubilized collagens from nile tilapia (Oreochromis niloticus) scale. Emirates Journal of Food and Agriculture, 31(2), 95–101. https://doi.org/10.9755/ejfa.2019.v31.i2.1911
Kong, J., & Yu, S. (2007). Fourier transform infrared spectroscopic analysis of protein secondary structures. Acta Biochimimica et Biophysica Sinica, 39(8), 549–559. https://doi.org/10.1111/j.1745-7270.2007.00320.x
Kryndushkin, D. S., Alexandrov, I. M., Ter-avanesyan, M. D., Vitaly, V., Chem, J. B., Kryndushkin, D. S., Alexandrov, I. M., Ter-avanesyan, M. D., & Kushnirov, V. V. (2003). Protein structure and folding : Yeast [ PSI + ] prion aggregates are formed by small sup35 polymers fragmented by hsp104. The Journal of Biological Chemistry, 278(49), 49636–49643. https://doi.org/10.1074/jbc.M307996200
Kumar, N. S., & Nazeer, R. A. (2013). Characterization of acid and pepsin soluble collagen from the skin of horse mackerels (Magalaspis cordyla) and croaker (Otolithes ruber). International Journal of Food Properties, 16(3), 613–621. https://doi.org/10.1080/10942912.2011.557796
Laemmli. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature Publishing Group, 227(5259), 680–685. http://www.mendeley.com/research/discreteness-conductance-chnge-n-bimolecular-lipid-membrane-presence-certin-antibiotics/
Lestari, V., Kusumaningrum, I., Zuraida, I., Diachanty, S., & Pamungkas, B. F. (2024). Pemanfaatan kepala dan tulang ikan bawis (Siganus canaliculatus) pada pengolahan kerupuk. Jurnal Pengolahan Hasil Perikanan Indonesia, 27(1), 16-26. http://dx.doi.org/10.17844/jphpi.v27i1.45014
Liu, D., Liang, L., Regenstein, J., & Zhou, P. (2012). Extraction and characterisation of pepsin-solubilised collagen from fins, scales, skins, bones and swim bladders of bighead carp (Hypophthalmichthys nobilis). Food Chem, 133(4), 1441–1448.
Mahboob, S. (2015). Isolation and characterization of collagen from fish waste material- skin, scales and fins of Catla catla and Cirrhinus mrigala. Journal of Food Science and Technology, 52(7), 4296–4305. https://doi.org/10.1007/s13197-014-1520-6
Matinong, A. M. E., Chisti, Y., Pickering, K. L., & Haverkamp, R. G. (2022). Collagen Extraction from Animal Skin. Biology, 11(6), 1–15. https://doi.org/10.3390/biology11060905
Matmaroh, K., Benjakul, S., Prodpran, T., Encarnacion, A., & Kishimura, H. (2011). Characteristics of acid soluble collagen and pepsin soluble collagen from scale of spotted golden goatfish (Parupeneus heptacanthus). Food Chemistry, 129(3), 1179–1186. https://doi.org/10.1016/j.foodchem.2011.05.099
Muyonga, J. H., Cole, C. G. B., & Duodu, K. G. (2004). Characterisation of acid soluble collagen from skins of young and adult nile perch (Lates niloticus). Food Chemistry, 85(1), 81–89. https://doi.org/10.1016/j.foodchem.2003.06.006
Nagai, T., & Suzuki, N. (2000). Isolation of collagen from fish waste material – skin, bone, and fins.Food Chemistry, 68(3), 277-281. https://doi.org/10.1016/S0308-8146(99)00188-0
Nagarajan, M., Benjakul, S., Prodpran, T., & Songtipya, P. (2012). Food hydrocolloids characteristics and functional properties of gelatin from splendid squid (Loligo formosana) skin as affected by extraction temperatures. Food Hydrocolloids, 29(2), 389–397. https://doi.org/10.1016/j.foodhyd.2012.04.001
Nalinanon, S., Benjakul, S., Kishimura, H., & Osako, K. (2011). Type I collagen from the skin of ornate threadfin bream (Nemipterus hexodon): characteristics and effect of pepsin hydrolysis. Food Chemistry, 125(2), 500–507.
Nollet, L. M. L. (2004). Handbook of Food Analysis Volume 1: Physical Characterization and Nutrient Analysis. In Public Health (Vol. 39). https://doi.org/10.1016/S0033-3506(25)80037-4
Nurhayati, T., Nurjanah., & Astiana, I. (2018, November 6-8). Characteristics of papain soluble collagen from redbelly yellowtail fusilier (Caesio cuning) [Conference session]. Sustainable Agriculture Transformation for The Nations Welfare of Indonesia and Malaysia, Serdang, Selangor, Malaysia. IOP Conference Series: earth and environmental science, 196012034.
Nurjanah., Baharuddin, T. I., & Nurhayati, T. (2021). Ekstraksi kolagen kulit ikan tuna sirip kuning (Thunnus albacares) menggunakan enzim pepsin dan papain. Jurnal Pengolahan Hasil Perikanan Indonesia, 24(2), 174-187. https://doi.org/10.17844/jphpi.v24i2.35410
Prastyo, D. T., Trilaksani, W., & Nurjanah. (2020). Aktivitas antioksidan hidrolisat kolagen kulit ikan nila (Oreochromis niloticus). Jurnal Pengolahan Hasil Perikanan Indonesia, 23(3), 423-433. https://doi.org/10.17844/jphpi.v23i3.31732
Ramli, A. R., Annisa, A. R., Bahmid, N. A., & Mustafa, M. D. (2020). Isolation of papain-soluble collagen from the skin of snake-head fish (Channa striata). Canrea Journal: Food Technology, Nutritions, and Culinary Journal, 3(2), 87–93. https://doi.org/10.20956/canrea.v3i2.367
Regenstein, J. M., & Zhou, P. (2006). Collagen and gelatin from marine by-products. In maximising the value of marine by-products. Woodhead Publishing Limited. https://doi.org/10.1533/9781845692087.2.279
Saito, M., Kunisaki, N., Urano, N., &Kimura, S. (2002). Collagen as the major edible component of sea cucumber (Stichopus japonicus). Food Chemistry and Toxicology, 67(4), 1319–1322. https://doi.org/10.1111/j.1365-2621.2002.tb10281.x
Schmidt, M. M., Dornelles, R. C. P., Mello, R. O., Kubota, E. H., Mazutti, M. A., Kempka, A. P., & Demiate, I. M. (2016). Collagen extraction process. International Food Research Journal, 23(3), 913–922.
Silvipriya, K., Kumar, K., Bhat, A., Kumar, B., John, A., & Lakshmanan, P. (2015). Collagen: animal sources and biomedical application. Journal of Applied Pharmarmaceutical Science, 5(3), 123–127. https://doi.org/10.7324/JAPS.2015.50322
Singh, P., Benjakul, S., Maqsood, S., & Kishimura, H. (2011). Isolation and characterisation of collagen extracted from the skin of striped catfish (Pangasianodon hypophthalmus). Food Chemistry, 124(1), 97–105. https://doi.org/10.1016/j.foodchem.2010.05.111
Subhan, F., Hussain, Z., Tauseef, I., Shehzad, A., & Wahid, F. (2021). A review on recent advances and applications of fish collagen. Critical Reviews in Food Science and Nutrition, 61(6), 1027–1037. https://doi.org/10.1080/10408398.2020.1751585
Sumandiarsa, I. K., Siregar, R. R., Ayu, K., & Dewi, S. (2020). Pengaruh metode pemasakan terhadap nilai sensori dan profil asam amino cakalang (Katsuwonus pelamis) masak. Jurnal Kelautan dan Perikanan Terapan, 3(2), 51–57. https://doi.org/10.15578/jkpt.v3i2.8719
Sumarlin, L. O., Nurbayti, S., & Fauziah, S. (2012). Penghambatan enzim pemecah protein (enzim papain) oleh ekstrak rokok, minuman beralkohol dan kopi secara in vitro. Jurnal Kimia Valensi, 2(3), 449 - 458. https://doi.org/10.15408/jkv.v2i3.116
Suseno, H. S., Pari, R. F., Ibrahim, B., Ramadhan, R. T. K., Listiana, D., Nurjannah, F., & Adha, A. S. A. (2023). Profil asam lemak minyak dari jeroan ikan nila dan mas dengan rasio pelarut yang berbeda. Jurnal Pengolahan Hasil Perikanan Indonesia, 26(3), 460-475. http://dx.doi.org/10.17844/jphpi.v26i3.45781
Suseno, S. H., Riskon, A. K., Jacoeb, A. M., Nurjanah, & Supinah, P. (2020). Ekstraksi dry rendering dan karakterisasi minyak ikan patin (Pangasius sp.) hasil samping industri filet di Lampung. Jurnal Pengolahan Hasil Perikanan Indonesia, 23(1), 38-46. https://doi.org/10.17844/jphpi.v23i1.30722
Tamilmozhi, S., Veeruraj, A., & Arumugam, M. (2013). Isolation and characterization of acid and pepsin-solubilized collagen from the skin of sailfish (Istiophorus platypterus). Food Research International, 54(2), 1499–1505. https://doi.org/10.1016/j.foodres.2013.10.002
Terzi, A., Gallo, N., Bettini, S., Sibillano, T., Altamura, D., Madaghiele, M., De Caro, L., Valli, L., Salvatore, L., Sannino, A., & Giannini, C. (2020). Sub- and supramolecular X-Ray characterization of engineered tissues from equine tendon, bovine dermis, and fish skin type-I collagen. Macromolecular Bioscience, 20(5), 1–18. https://doi.org/10.1002/mabi.202000017
Tylingo, R., & Mania, S. (2016). Isolation and characterization of acid soluble collagen from the skin of African catfish (Clarias gariepinus), salmon (Salmo salar) and baltic cod (Gadus morhua). Journal of Biotechnology & Biomaterials, 6(2), 1-6. https://doi.org/10.4172/2155-952x.1000234
Waters. (2012). Acquity UPLC H class and H class bio amino acid analysis system guide. Waters Corporation. https://www.waters.com/webassets/cms/support/docs/acq_uplc_h-class_aaa_sysgd_rev_b.pdf
Widiyanto., Uju., & Nurilmala, M. (2022). Karakteristik kolagen dari kulit dan sisik ikan coklatan, swanggi, dan kurisi sebagai bahan gelatin. Jurnal Pengolahan Hasil Perikanan Indonesia, 25(3), 512-527. http://dx.doi.org/10.17844/jphpi.v25i3.43598
Woo, J. W., Yu, S. J., Cho, S. M., Lee, Y. B., & Kim, S. B. (2008). Extraction optimization and properties of collagen from yellowfin tuna (Thunnus albacares) dorsal skin. Food Hydrocolloids, 22(5), 879–887. https://doi.org/10.1016/j.foodhyd.2007.04.015
Yanti, F., Dharmayanti, N., & Suryanti. (2022). Aktivitas antioksidan kolagen dari kulit ikan patin (Pangasius sp.) dengan enzim bromelin kasar kulit nanas (Ananas comosus L.). Jurnal Pengolahan Hasil Perikanan Indonesia, 25(1), 88-96. https://doi.org/10.17844/jphpi.v25i1.36731
Zamorano-Apodaca, J. C., García-Sifuentes, C. O., Carvajal-Millán, E., Vallejo-Galland, B., Scheuren-Acevedo, S. M., & Lugo-Sánchez, M. E. (2020). Biological and functional properties of peptide fractions obtained from collagen hydrolysate derived from mixed by-products of different fish species. Food Chemistry, 331(10), 127350. https://doi.org/10.1016/j.foodchem.2020.127350
Zeng, S. kui, Zhang, C. hua, Lin, H., Yang, P., Hong, P. zhi, & Jiang, Z. (2009). Isolation and characterisation of acid-solubilised collagen from the skin of nile tilapia (Oreochromis niloticus). Food Chemistry, 116(4), 879–883. https://doi.org/10.1016/j.foodchem.2009.03.038
Zhang, F., Wang, A., Li, Z., He, S., & Shao, L. (2011). Preparation and characterisation of collagen from freshwater fish scales. Food and Nutrition Sciences, 02(08), 818–823. https://doi.org/10.4236/fns.2011.28112
Authors
Afifah A., Suparno O., Haditjaroko L., Tarman K., Setiyono A., & Nugraha A. W. (2024). Isolation and characterization of collagen from salmon (Salmo salar) skin using papain enzyme: Isolasi dan karakterisasi kolagen dari kulit ikan salmon (Salmo salar) menggunakan enzim papain. Jurnal Pengolahan Hasil Perikanan Indonesia, 27(6), 536-552. https://doi.org/10.17844/jphpi.v27i6.53285
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