Potensi ulvan dari Ulva lactuca sebagai sumber antioksidan Potential of ulvan from Ulva lactuca as an antioxidant source
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Accepted
27 February 2024
Published
13 March 2024
Keywords:
-
DPPH, FTIR, graphimetric, IC50, spectrophotometric
Abstract
Ulva lactuca is a type of marine macroalga that produces a sulfated hydrocolloid known as ulvan, which has been identified as a potent antioxidant. This finding suggests that the U. lactuca cell wall has potential applications in the development of antioxidant-rich products. This study aimed to investigate the influence of heating time in an autoclave (15 and 20 min at 121 °C) on the yield, sugar and sulfate contents, and antioxidant activity of ulvan. The yield of ulvan, as well as the sugar and sulfate contents, were determined using graphimetric and spectrophotometric techniques, respectively, and the functional groups and antioxidants of the dried extract were analyzed using Fourier Transform Infrared Spectroscopy (FTIR) and the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) method, respectively. The analysis was conducted on the oven-dried samples. Ulvan can be obtained by extracting it at a temperature of 121 °C for 15 min; ulvan extraction yielded a percentage of 1.98, with a total sugar content of 46.06±0.16% and a sulfate content of 21.53±1.16%. Infrared spectroscopy revealed the presence of wavenumbers 850 and 790 cm-1, which are indicative of the presence of ulvans. DPPH assay yielded an IC50 value of 469 ppm, which was categorized as a relatively weak antioxidant.
References
Adrien, A., Bonnet, A., Dufour, D., Bauduoin, S., Maugard, T., & Bridiau, N. (2017). Pilot production of ulvans from Ulva sp. and their effects on hyaluronan and collagen production in cultured dermal fibroblasts. Carbohydrate Polymers, 157, 1306-1314. https://doi.org/10.1016/j.carbpol.2016.11.014
Aguilar-Briseño, J.A., Cruz-Suarez, L.E., Sassi, J.F., Ricque-Marie, D., Zapata-Benavides, P., Mendoza-Gamboa, E., Rodríguez-Padilla, C., & Trejo-Avila, L.M. (2015). Sulphated polysaccharides from Ulva clathrata and Cladosiphon okamuranus seaweeds both inhibit viral attachment/entry and cell-cell fusion, in NDV infection. Marine Drugs, 13, 697-712. http://doi:10.3390/md13020697
Alves, A., Sousa, R.A., & Reis, R.L. (2013). A practical perspective on ulvan extracted from green algae. Journal of Applied Phycology, 25, 407-424. http://doi:10.1007/s10811-012-9875-4
American Society for Testing Material. (2013). ASTM E1258-98: Standar Practice for General Technique for Obtaining Infrared Spectra for Qualitative Analysis. American Society for Testing Material.
Angell, A.R., Mata, L., de Nys, R., & Paul, N.A. (2014). Variation in amino acid content and its relationship to nitrogen content and growth rate in Ulva ohnoi (Chlorophyta). Journal of Applied Phycology, 50, 216-226. https://doi.org/10.1111/jpy.12154
Aryanti, N., Aininu, N., & Fathia, M.W. (2016). Ekstraksi dan karakterisasi klorofil dari daun suji (Pleomele angustifolia) sebagai pewarna pangan alami. Jurnal Aplikasi Teknologi Pangan, 5(4), 129-139. https://doi.org/10.17728/jatp.183
Barcellos, P.G., Rodrigues, J.A.G., de Queiroz, I.N.L., de Araújo, I.W.F., Benevides, N.M.B., & de Souza Mourão, P.A. (2018). Structural and physical-chemical analyses of sulfated polysaccharides from the sea lettuce Ulva lactuca and their effects on thrombin generation. Acta Scientiarum. Biological Sciences, 40, 1-12. https://doi.org/10.4025/actascibiolsci.v40i1.34916
Costa, C., Alves, A., Pinto, P.R., Sousa, R.A., da Silva, E.A.B., & Reis, R.L. (2012). Characterization of ulvan extracts to assess the effect of different steps in the extraction procedure. Carbohydrate Polymers, 88(2), 537-546. http://doi:10.1016/j.carbpol.2011.12.041
Cunha, L., & Grenha, A. (2016). Sulfated seaweed polysaccharides as multifunctional materials in drug delivery applications. Marine Drugs, 14, 1-42. http://doi:10.3390/md14030042
Da Costa, J.F., Merdekawati, W., & Out, F.R. (2018). Analisis proksimat, antioksidan, dan komposisi pigmen Ulva lactuca L dari perairan Kukup. Jurnal Teknologi Pangan dan Gizi, 17 (1), 1-17.
Dubois, M., Gilles, K.A, Hamilton, J.K, Rebers, P.A, & Smith, F. (1956). Colorimetric method for determination of sugar and related substances. Analysis Chemical, 6, 281-350.
Farasat, M., Khavari-Nejad, R.-A, Nabavi, S.M.B., & Namjooyan, F. (2014). Antioxidant activity, total phenolics and flavonoid contents of some edible green seaweeds from northern coasts of the Persian Gulf. Iranian Journal of Pharmaceutical Research, 13(1), 163-170.
Figueira, T.A., da Silva, A.J.R., Enrich-Prast, A., Yoneshigue-Valentin, Y., & de Oliveira, V.P. (2020). Structural characterization of ulvan polysaccharide from cultivated and collected Ulva fasciata (Chlorophyta). Advances in Bioscience and Biotechnology, 11, 206-216. http://doi.org.10.4236/abb.2020.11.50.16
Glasson, C.R.K., Sims, I.M., Carnachan, S.M., de Nys, R., & Magnusson, M. (2017). A cascading biorefinery process targeting sulfated polysaccharides (ulvan) from Ulva ohnoi. Algal Research, 27, 383–391. https://doi.org/10.1016/j.algal.2017.07.001
Gross, J. (1991). Pigment invegetable, chlorophylls and carotenoids. Van Nostrand Reinhold.
He, J., Xu, Y., Chen, H, & Sun, P. (2016). Extraction, structural characterization, and potential antioxidant activity of the polysaccharides from four seaweeds. International Journal of Moleculer Sciences, 17(12), 1988. https://doi.org/10.3390/ijms17121988
Hernández-Garibay, E., Zertuche-Gonzáles, J.A., & Pacheco-Ruíz, I. (2011). Isolation and chemical characterization of algal polysaccharides from the green seaweed Ulva lactuca (Roth) C. Agardh. Journal of Applied Phycology, 23, 537-542. http://doi:10.1007/s10811-010-9629-0
Huimin, Q., Tingting, Z., Quanbin, Z., Zhien, L., Zengqin, Z., & Ronge, X. (2005). Antioxidant activity of different molecular weight sulfated polysaccharides from Ulva pertusa (Chlorophyta). Journal of Applied Phycology, 17, 527-534.
Hussein, M.H., Hamouda, R.A., El-Naggar, N.E., & Karim-Eldeen, M.A. (2015). Characterization, antioxidant potentiality and biological activities of the polysaccharide ulvan extracted from the marine macroalga Ulva spp. ournal of Agricultural Chemistry and Biotechnology, 6(9), 373-392. http://doi.org/10.21608/jacb.2015.48435
Ibrahim, M.I.A., Amer, M.S., Ibrahim, H.A.H., & Zaghloul, E.H. (2022). Considerable production of ulvan from Ulva lactuca with special emphasis on its antimicrobial and anti-fouling properties. Applied Biochemistry and Biotechnology, 194, 3097–3118.http://doi.org/10.1007/s12010-022-03867-y
Jayapal, N., Samanta, A., Kolte, A.P., Senani, S., Sridhar, M., Suresh, K., & Sampath, K. (2013). Value addition to sugarcane bagasse: Xylan extraction and its process optimization for xylooligosaccharides production. Indrustial Crops Products, 42, 14–24. https://doi.org/10.1016/j.indcrop.2012.05.019
Khuluq, A.D.S., Widjanarko, S.B., & Murtini, E.S. (2007). Ekstraksi dan betasianin daun darah (Alternanthera dentata): Kajian perbandingan pelarut air:etanol dan suhu ekstraksi. Jurnal Teknologi Pertanian, 8(3), 172-181.
Kidgell, J.T., Magnusson, M., de Nys, R., & Glasson, C.R.K. (2019). Ulvan: A systematic review of extraction, composition and function. Algal Research, 39. http://doi.org/10.1016/j.algal.2019.101422
Kim, W. J., Kim, S. M., Kim, H. G., Oh, H. R., Lee, K. B.,Lee Y. K, & Park, Y. I. (2007). Purification and anticoagulant activity of fucoidan from Korean Undaria pinnatifida. Journal Alga, 22(3), 247-252.
Kurniasih, S.D., Pramesti, R., & Ridlo, A. (2014). Penentuan aktivitas antioksidan ekstrak rumput laut Ulva sp. dari pantai Krakal-Yogyakarta. Journal of Marine Research, 3(4), 617-626. https://doi.org/10.14710/jmr.v3i4.11423
Kurniawan, R., Nurjanah, Jacoeb, A. M., Abdullah, A., & Pertiwi, R., M. (2019). Karakteristik garam fungsional dari rumput laut hijau. Jurnal Pengolahan Hasil Perikanan Indonesia. 22(3): 573-580. https://doi.org/10.17844/jphpi.v22i3.29320
Kwon, M.J., & Nam, T.J. (2007). A polysaccharide of the marine Capsosiphon fulvescens induces apoptosis in AGS gastric cancer cells via an IGF-IR-mediated P13K/Akt pathway. Cellbiology International, 31, 768-775. https://doi.org/10.1016/j.cellbi.2007.01.010
Lahaye, M., & Kaeffer, B. (1997). Seaweed dietary fibres: structure, psycho-chemical and biological properties relevant to intestinal physiology. Sciences des Aliments, 17, 563-584.
Lahaye, M., & Robic, A. (2007). Structure and functional properties of ulvan, a polysaccharide from green seaweed. Biomacromolecules, 8, 1765-1774. http://doi: 10.1021/bm061185q.
Lee, N.Y., Yunus, M.A.C, Idham, Z., Ruslan, M.S.H., Aziz, A.H.A., & Irwansyah, N. (2016). Extraction and identification of bioactive compound from agarwood leaves. Second International Conference on Chemical Engineering (ICCE) 162: 1-6. http://doi:10.1088/1757-899X/162/1/012028.
Li, W., Jiang, N., Li, B., Wan, M., Chang, X., Liu, H., Zhang, L., Yin, S., Qi, H., & Liu, S. (2018). Antioxidant activity of purified ulvan in hyperlipidemic mice. International Journal of Biological Macromolecules, 113, 971-975. https://doi.org/10.1016/j.ijbiomac.2018.02.104
Maray, S.O., Mohamed, S. M., Abdel‑Kareem, M.S.M., Mabrouk, M.E.M., El‑Halmouch, Y., & Makhlof, M.E.M. (2023). In vitro assessment of antiviral, antimicrobial, antioxidant and anticancer activities of Ulvan Extracted from the Green Seaweed Ulva lactuca. Thalassas: An International Journal of Marine Sciences, 39779–790. https://doi.org/10.1007/s41208-023-00584-z
Mezghani, S., Bourguiba, I., Hfaiedh, I., & Amri, M. (2013). Antioxidant potential of Ulva rigida extracts: protection of HeLa Cells against H2O2 cytotoxicity. The Biological Bulletin, 225, 1–7. http://doi:10.1086/BBLv225n1p1
Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26(2), 211-219.
Mo’o, F.R.C., Wilar, G., Devkota, H.P., & Wathoni, N. (2020). Ulvan, a polysaccharide from macroalga Ulva sp.: A review of chemistry, biological activities and potential for food and biomedical applications. Applied Sciences, 10(16), 5488. https://doi.org/10.3390/app10165488
Murphy, V., Hughes, H., & McLoughlin, P. (2008). Comparative study of chromium biosorption by red, green and brown seaweed biomass. Chemosphere, 70, 1128–1134. http://doi: 10.1016/j.chemosphere.2007.08.015
Nurjanah, Abdullah. A., & Nufus, C. (2018). Karakteristik sediaan garam Ulva lactuca dari perairan sekotong Nusa Tenggara Barat bagi pasien hipertensi. Jurnal Pengolahan Hasil Perikanan Indonesia, 21(1), 109-117.
Nurjanah, Jacoeb, A. M., Ramlan, & Abdullah, A. (2020). Penambahan genjer (Limnocharis flava) pada pembuatan garam rumput laut hijau untuk penderita hipertensi. Jurnal Pengolahan Hasil Perikanan Indonesia, 23(3), 459-469.
Nurjanah, Ramlan, Jacoeb, A. M., & Seulalae, A. V. (2023). Komposisi kimia tepung dan aktivitas antioksidan ekstrak Ulva lactuca dan genjer (Limnocharis flava) sebagai bahan baku pembuatan garam rumput laut. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan, 18(1), 63-74. http://dx.doi.org/10.15578/jpbkp.v18i1.931
Nusaibah, Muhammad, T., Pangestika, W., Siregar, A. N., & Utami, K. D. (2023). Karakteristik serum wajah dari sediaan filtrat rumput laut Eucheuma cottonii dan Ulva lactuca. Jurnal Pengolahan Hasil Perikanan Indonesia, 26(3), 545-559. http://dx.doi.org/10.17844/jphpi.v26i3.46874
Pereira, L., Amado, A.M., Critchley, A.T., Van de Velde, F., & Ribeiro-Claro, P.J. (2009). Identification of selected seaweed polysaccharides (phycocolloids) by vibrational spectroscopy (FTIR-ATR and FT-Raman). Food Hydrocolloids, 23, 1903–1909. https://doi.org/10.1016/j.foodhyd.2008.11.014
Pratista, I.M.I., Suhendra, L., & Wrasiati, L.P. (2017). Karakteristik pewarna alami pada ekstrak Sargassum polycystum dengan konsentrasi pelarut etanol dan lama maserasi yang berbeda. Jurnal Rekayasa dan Manajemen Agroindustri, 5(4), 51-60.
Ramadhan, W., Uju, Hardiningtyas, S.D., Pari, R.F., Nurhayati, & Sevica, D. (2022). Ekstraksi polisakarida Ulvan dari rumput laut Ulva lactuca berbantu gelombang ultrasonik pada suhu rendah. Jurnal Pengolahan Hasil Perikanan Indonesia, 25(1), 132-142. http://dx.doi.org/ 10.17844/jphpi.v25i1.40407
Ray, B., & Lahaye, M. (1995). Cell-wall polyscharides from the marine algae Ulva rigida (ulvales, chlorophyta). Extraction and chemical composition. Carboydridrate Research, 274, 251-261.
Robic, A., Gaillard, C., Sassi, J.F., Leral, Y., & Lahaye, M. (2009a). Ultrastructure of ulvan: A polysaccharide from green seaweeds. Biopolymers, 91, 652–664. https://doi.org/10.1002/bip.21195
Robic, A., Rondeau-Mouro, C., Sassi, J.F., Lerat, Y., & Lahaye, M. (2009b). Structure and interactions of ulvan in the cell wall of the marine green algae Ulva rotundata (Ulvales, Chlorophyceae). Carbohydrate Polymers, 77, 206–216. https://doi.org/10.1016/j.carbpol.2008.12.023
Robic, A., Bertrand, D., Sassi, J.F., Lerat, Y., & Lahaye, M. (2009c). Determination of the chemical composition of ulvan, a cell wall polysaccharide from Ulva spp. (Ulvales, Chlorophyta) by FT-IR and chemometrics. Journal of Applied Phycology, 21, 451–456. http://doi:10.1007/s10811-008-9390-9
Santi, R.A., Sunarti, T.C., Santoso, D., & Triwisari, D.A. (2012). Komposisi kimia dan profil polisakarid rumput laut hijau. Jurnal Akuatika, 3(2), 105-114.
Schwanninger, M., Rodrigues, J., Pereira, H., & Hinterstoisser, B. (2004). Effects of short- time vibratory ball milling on the shape of FT-IR spectra of wood and cellulose. Vibrational Spectroscopy, 36, 23–40. https://doi.org/10.1016/j.vibspec.2004.02.003
Sianipar, E.A., Satriawan, N., Sumartono, J., & Kambira, P.F.A. (2022). Pengujian aktivitas antioksidan makro alga Sumbawa dalam hubungannya dengan kandungan senyawa bioaktif dan efek farmakologi. Jurnal Riset Kesehatan Nasional, 6(2), 151-157.
Sihono, Sinurat, E., Fateha, Supriyanto, A., Suryaningrum, T. D., Nurhayati, Fransiska, D., Utomo, B. S. B., Subaryono, Sedayu, B. B., Waryanto, Nurjanah, Ramadhan, W., Fadillah, H. M., & Muzayyanah, A. L. (2023). Optimasi formula nori-like product dari Ulva spp., Gracilaria sp., dan gliserol menggunakan metode mixture design. Jurnal Pengolahan Hasil Perikanan Indonesia, 26(3), 433-447. http://dx.doi.org/10.17844/jphpi.v26i3.48337
Sun, L., Wang, L., Li, J., & Liu, H. (2014). Characterization and antioxidant activities of degraded polysaccharides from two marine chrysophyta. Food Chemistry, 160, 1-7. https://doi.org/10.1016/j.foodchem.2014.03.067
Suresh, V., Senthilkumar, N., Thangam, R., Rajkumarc, M., Anbazhagana, C., Rengasamy, R., Gunasekaranc, P., Kannand, S., & Palanib, P. (2013). Separation, purification and preliminary characterization of sulfated polysaccharides from Sargassum plagiophyllum and its in vitro anticancer and antioxidant activity. Process Biochemistry, 48, 364–373. http://dx.doi.org/10.1016%2Fj.procbio.2012.12.014
Tako, M., Tamanaha, M., Tamashiro, Y., & Uechi, S. (2015). Structure of ulvan isolated from the edible green seaweed, Ulva pertusa. Advances in Bioscience and Biotechnology, 6, 645-655. http://dx.doi.org/10.4236/abb.2015.610068.
Tsubaki, S., Oono, K., Hiraoka, M., Onda, A., & Mitani, T. (2016). Microwave-assisted hydrothermal extraction of sulfated polysaccharides from Ulva spp. and Monostroma latissimum. Food Chemistry, 210, 311–316. https://doi.org/10.1016/j.foodchem.2016.04.121
Valentine, G., Sumardianto, & Wijayanti, I. (2020). Karakteristik nori dari rumput laut Ulva lactuca dan Gelidium sp. Jurnal Pengolahan Hasil Perikanan Indonesia, 23(2): 295-302. https://doi.org/10.17844/jphpi.v23i2.32340
Venkatesan, J., Lowe, B., Anil, S., Manivasagan, P., Kheraif, A.A.A., Kang, K.H., & Kim, S.K. (2015). Seaweed polysaccharides and their potential biomedical application. Starch-Staerke, 67, 381-390. https://doi.org/10.1002/star.201400127
Wahlström, N., Nylander, F., Malmhäll-Bah, E., Sjövold, K., Edlunda, U., Westman, G., & Albers, E. (2020). Composition and structure of cell wall ulvans recovered from Ulva spp. along the Swedish west coast. Carbohydrate Polymers, 233, 1-9. https://doi.org/10.1016/j.carbpol.2020.115852
Weafer, C. (1996). The Food Chemistry Laboratory. CRC Press.
Widowaty, W., Setiawan, S., & Perdana, W.W. (2020). Aktivitas antioksidan ekstrak Gracilaria sp. dan Ulva sp. dari pantai Sayang Heulang. Agroscience, 10(2), 203-209.
Wijesekara, I., Pangestuti, R., & Kim, S.-K. (2011). Biological activities and potential health benefits of sulfated polysaccharides derive from marine algae. Carbohydrate Polymers, 84, 14-21. https://doi.org/10.1016/j.carbpol.2010.10.062
Wu, H.Z., Wang, J.M., Yang, H., Li, G.Q., Zeng, Y.H., Xia, W., Li, Z.G., & Qian, M.R. (2017). Development and application of an in-cell clean up pressurized liquid extraction with ultra-high-performance liquid chromatography-tandem mass spectrometry to detect prohibited antiviral agents sensitively in livesstock and poultry feces. Journal of Chromatography, 1488, 10-16. https://doi.org/10.1016/j.chroma.2017.01.070
Yaich, H., Garna, H., Besbes, S., Paquot, M., Blecker, C., & Attia, H. (2013). Effect of extraction conditions on the yield and purity of ulvan extracted from Ulva lactuca. Food Hydrocolloid, 31(2), 375-382. https://doi.org/10.4025/actascibiolsci.v40i1.34916
Yaich, H., Amira, A.B. , Abbes, F., Bouaziz, M., Besbes, S., Richel, A., Blecker, C., Attia, H., & Garna, H. (2017). Effect of extraction procedures on structural, thermal and antioxidant properties of ulvan from Ulva lactuca collected in Monastir coast. International Journal of Biological Macromolecules, 105(2), 1430-1439. https://doi.org/10.1016/j.ijbiomac.2017.07.141
Aguilar-Briseño, J.A., Cruz-Suarez, L.E., Sassi, J.F., Ricque-Marie, D., Zapata-Benavides, P., Mendoza-Gamboa, E., Rodríguez-Padilla, C., & Trejo-Avila, L.M. (2015). Sulphated polysaccharides from Ulva clathrata and Cladosiphon okamuranus seaweeds both inhibit viral attachment/entry and cell-cell fusion, in NDV infection. Marine Drugs, 13, 697-712. http://doi:10.3390/md13020697
Alves, A., Sousa, R.A., & Reis, R.L. (2013). A practical perspective on ulvan extracted from green algae. Journal of Applied Phycology, 25, 407-424. http://doi:10.1007/s10811-012-9875-4
American Society for Testing Material. (2013). ASTM E1258-98: Standar Practice for General Technique for Obtaining Infrared Spectra for Qualitative Analysis. American Society for Testing Material.
Angell, A.R., Mata, L., de Nys, R., & Paul, N.A. (2014). Variation in amino acid content and its relationship to nitrogen content and growth rate in Ulva ohnoi (Chlorophyta). Journal of Applied Phycology, 50, 216-226. https://doi.org/10.1111/jpy.12154
Aryanti, N., Aininu, N., & Fathia, M.W. (2016). Ekstraksi dan karakterisasi klorofil dari daun suji (Pleomele angustifolia) sebagai pewarna pangan alami. Jurnal Aplikasi Teknologi Pangan, 5(4), 129-139. https://doi.org/10.17728/jatp.183
Barcellos, P.G., Rodrigues, J.A.G., de Queiroz, I.N.L., de Araújo, I.W.F., Benevides, N.M.B., & de Souza Mourão, P.A. (2018). Structural and physical-chemical analyses of sulfated polysaccharides from the sea lettuce Ulva lactuca and their effects on thrombin generation. Acta Scientiarum. Biological Sciences, 40, 1-12. https://doi.org/10.4025/actascibiolsci.v40i1.34916
Costa, C., Alves, A., Pinto, P.R., Sousa, R.A., da Silva, E.A.B., & Reis, R.L. (2012). Characterization of ulvan extracts to assess the effect of different steps in the extraction procedure. Carbohydrate Polymers, 88(2), 537-546. http://doi:10.1016/j.carbpol.2011.12.041
Cunha, L., & Grenha, A. (2016). Sulfated seaweed polysaccharides as multifunctional materials in drug delivery applications. Marine Drugs, 14, 1-42. http://doi:10.3390/md14030042
Da Costa, J.F., Merdekawati, W., & Out, F.R. (2018). Analisis proksimat, antioksidan, dan komposisi pigmen Ulva lactuca L dari perairan Kukup. Jurnal Teknologi Pangan dan Gizi, 17 (1), 1-17.
Dubois, M., Gilles, K.A, Hamilton, J.K, Rebers, P.A, & Smith, F. (1956). Colorimetric method for determination of sugar and related substances. Analysis Chemical, 6, 281-350.
Farasat, M., Khavari-Nejad, R.-A, Nabavi, S.M.B., & Namjooyan, F. (2014). Antioxidant activity, total phenolics and flavonoid contents of some edible green seaweeds from northern coasts of the Persian Gulf. Iranian Journal of Pharmaceutical Research, 13(1), 163-170.
Figueira, T.A., da Silva, A.J.R., Enrich-Prast, A., Yoneshigue-Valentin, Y., & de Oliveira, V.P. (2020). Structural characterization of ulvan polysaccharide from cultivated and collected Ulva fasciata (Chlorophyta). Advances in Bioscience and Biotechnology, 11, 206-216. http://doi.org.10.4236/abb.2020.11.50.16
Glasson, C.R.K., Sims, I.M., Carnachan, S.M., de Nys, R., & Magnusson, M. (2017). A cascading biorefinery process targeting sulfated polysaccharides (ulvan) from Ulva ohnoi. Algal Research, 27, 383–391. https://doi.org/10.1016/j.algal.2017.07.001
Gross, J. (1991). Pigment invegetable, chlorophylls and carotenoids. Van Nostrand Reinhold.
He, J., Xu, Y., Chen, H, & Sun, P. (2016). Extraction, structural characterization, and potential antioxidant activity of the polysaccharides from four seaweeds. International Journal of Moleculer Sciences, 17(12), 1988. https://doi.org/10.3390/ijms17121988
Hernández-Garibay, E., Zertuche-Gonzáles, J.A., & Pacheco-Ruíz, I. (2011). Isolation and chemical characterization of algal polysaccharides from the green seaweed Ulva lactuca (Roth) C. Agardh. Journal of Applied Phycology, 23, 537-542. http://doi:10.1007/s10811-010-9629-0
Huimin, Q., Tingting, Z., Quanbin, Z., Zhien, L., Zengqin, Z., & Ronge, X. (2005). Antioxidant activity of different molecular weight sulfated polysaccharides from Ulva pertusa (Chlorophyta). Journal of Applied Phycology, 17, 527-534.
Hussein, M.H., Hamouda, R.A., El-Naggar, N.E., & Karim-Eldeen, M.A. (2015). Characterization, antioxidant potentiality and biological activities of the polysaccharide ulvan extracted from the marine macroalga Ulva spp. ournal of Agricultural Chemistry and Biotechnology, 6(9), 373-392. http://doi.org/10.21608/jacb.2015.48435
Ibrahim, M.I.A., Amer, M.S., Ibrahim, H.A.H., & Zaghloul, E.H. (2022). Considerable production of ulvan from Ulva lactuca with special emphasis on its antimicrobial and anti-fouling properties. Applied Biochemistry and Biotechnology, 194, 3097–3118.http://doi.org/10.1007/s12010-022-03867-y
Jayapal, N., Samanta, A., Kolte, A.P., Senani, S., Sridhar, M., Suresh, K., & Sampath, K. (2013). Value addition to sugarcane bagasse: Xylan extraction and its process optimization for xylooligosaccharides production. Indrustial Crops Products, 42, 14–24. https://doi.org/10.1016/j.indcrop.2012.05.019
Khuluq, A.D.S., Widjanarko, S.B., & Murtini, E.S. (2007). Ekstraksi dan betasianin daun darah (Alternanthera dentata): Kajian perbandingan pelarut air:etanol dan suhu ekstraksi. Jurnal Teknologi Pertanian, 8(3), 172-181.
Kidgell, J.T., Magnusson, M., de Nys, R., & Glasson, C.R.K. (2019). Ulvan: A systematic review of extraction, composition and function. Algal Research, 39. http://doi.org/10.1016/j.algal.2019.101422
Kim, W. J., Kim, S. M., Kim, H. G., Oh, H. R., Lee, K. B.,Lee Y. K, & Park, Y. I. (2007). Purification and anticoagulant activity of fucoidan from Korean Undaria pinnatifida. Journal Alga, 22(3), 247-252.
Kurniasih, S.D., Pramesti, R., & Ridlo, A. (2014). Penentuan aktivitas antioksidan ekstrak rumput laut Ulva sp. dari pantai Krakal-Yogyakarta. Journal of Marine Research, 3(4), 617-626. https://doi.org/10.14710/jmr.v3i4.11423
Kurniawan, R., Nurjanah, Jacoeb, A. M., Abdullah, A., & Pertiwi, R., M. (2019). Karakteristik garam fungsional dari rumput laut hijau. Jurnal Pengolahan Hasil Perikanan Indonesia. 22(3): 573-580. https://doi.org/10.17844/jphpi.v22i3.29320
Kwon, M.J., & Nam, T.J. (2007). A polysaccharide of the marine Capsosiphon fulvescens induces apoptosis in AGS gastric cancer cells via an IGF-IR-mediated P13K/Akt pathway. Cellbiology International, 31, 768-775. https://doi.org/10.1016/j.cellbi.2007.01.010
Lahaye, M., & Kaeffer, B. (1997). Seaweed dietary fibres: structure, psycho-chemical and biological properties relevant to intestinal physiology. Sciences des Aliments, 17, 563-584.
Lahaye, M., & Robic, A. (2007). Structure and functional properties of ulvan, a polysaccharide from green seaweed. Biomacromolecules, 8, 1765-1774. http://doi: 10.1021/bm061185q.
Lee, N.Y., Yunus, M.A.C, Idham, Z., Ruslan, M.S.H., Aziz, A.H.A., & Irwansyah, N. (2016). Extraction and identification of bioactive compound from agarwood leaves. Second International Conference on Chemical Engineering (ICCE) 162: 1-6. http://doi:10.1088/1757-899X/162/1/012028.
Li, W., Jiang, N., Li, B., Wan, M., Chang, X., Liu, H., Zhang, L., Yin, S., Qi, H., & Liu, S. (2018). Antioxidant activity of purified ulvan in hyperlipidemic mice. International Journal of Biological Macromolecules, 113, 971-975. https://doi.org/10.1016/j.ijbiomac.2018.02.104
Maray, S.O., Mohamed, S. M., Abdel‑Kareem, M.S.M., Mabrouk, M.E.M., El‑Halmouch, Y., & Makhlof, M.E.M. (2023). In vitro assessment of antiviral, antimicrobial, antioxidant and anticancer activities of Ulvan Extracted from the Green Seaweed Ulva lactuca. Thalassas: An International Journal of Marine Sciences, 39779–790. https://doi.org/10.1007/s41208-023-00584-z
Mezghani, S., Bourguiba, I., Hfaiedh, I., & Amri, M. (2013). Antioxidant potential of Ulva rigida extracts: protection of HeLa Cells against H2O2 cytotoxicity. The Biological Bulletin, 225, 1–7. http://doi:10.1086/BBLv225n1p1
Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26(2), 211-219.
Mo’o, F.R.C., Wilar, G., Devkota, H.P., & Wathoni, N. (2020). Ulvan, a polysaccharide from macroalga Ulva sp.: A review of chemistry, biological activities and potential for food and biomedical applications. Applied Sciences, 10(16), 5488. https://doi.org/10.3390/app10165488
Murphy, V., Hughes, H., & McLoughlin, P. (2008). Comparative study of chromium biosorption by red, green and brown seaweed biomass. Chemosphere, 70, 1128–1134. http://doi: 10.1016/j.chemosphere.2007.08.015
Nurjanah, Abdullah. A., & Nufus, C. (2018). Karakteristik sediaan garam Ulva lactuca dari perairan sekotong Nusa Tenggara Barat bagi pasien hipertensi. Jurnal Pengolahan Hasil Perikanan Indonesia, 21(1), 109-117.
Nurjanah, Jacoeb, A. M., Ramlan, & Abdullah, A. (2020). Penambahan genjer (Limnocharis flava) pada pembuatan garam rumput laut hijau untuk penderita hipertensi. Jurnal Pengolahan Hasil Perikanan Indonesia, 23(3), 459-469.
Nurjanah, Ramlan, Jacoeb, A. M., & Seulalae, A. V. (2023). Komposisi kimia tepung dan aktivitas antioksidan ekstrak Ulva lactuca dan genjer (Limnocharis flava) sebagai bahan baku pembuatan garam rumput laut. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan, 18(1), 63-74. http://dx.doi.org/10.15578/jpbkp.v18i1.931
Nusaibah, Muhammad, T., Pangestika, W., Siregar, A. N., & Utami, K. D. (2023). Karakteristik serum wajah dari sediaan filtrat rumput laut Eucheuma cottonii dan Ulva lactuca. Jurnal Pengolahan Hasil Perikanan Indonesia, 26(3), 545-559. http://dx.doi.org/10.17844/jphpi.v26i3.46874
Pereira, L., Amado, A.M., Critchley, A.T., Van de Velde, F., & Ribeiro-Claro, P.J. (2009). Identification of selected seaweed polysaccharides (phycocolloids) by vibrational spectroscopy (FTIR-ATR and FT-Raman). Food Hydrocolloids, 23, 1903–1909. https://doi.org/10.1016/j.foodhyd.2008.11.014
Pratista, I.M.I., Suhendra, L., & Wrasiati, L.P. (2017). Karakteristik pewarna alami pada ekstrak Sargassum polycystum dengan konsentrasi pelarut etanol dan lama maserasi yang berbeda. Jurnal Rekayasa dan Manajemen Agroindustri, 5(4), 51-60.
Ramadhan, W., Uju, Hardiningtyas, S.D., Pari, R.F., Nurhayati, & Sevica, D. (2022). Ekstraksi polisakarida Ulvan dari rumput laut Ulva lactuca berbantu gelombang ultrasonik pada suhu rendah. Jurnal Pengolahan Hasil Perikanan Indonesia, 25(1), 132-142. http://dx.doi.org/ 10.17844/jphpi.v25i1.40407
Ray, B., & Lahaye, M. (1995). Cell-wall polyscharides from the marine algae Ulva rigida (ulvales, chlorophyta). Extraction and chemical composition. Carboydridrate Research, 274, 251-261.
Robic, A., Gaillard, C., Sassi, J.F., Leral, Y., & Lahaye, M. (2009a). Ultrastructure of ulvan: A polysaccharide from green seaweeds. Biopolymers, 91, 652–664. https://doi.org/10.1002/bip.21195
Robic, A., Rondeau-Mouro, C., Sassi, J.F., Lerat, Y., & Lahaye, M. (2009b). Structure and interactions of ulvan in the cell wall of the marine green algae Ulva rotundata (Ulvales, Chlorophyceae). Carbohydrate Polymers, 77, 206–216. https://doi.org/10.1016/j.carbpol.2008.12.023
Robic, A., Bertrand, D., Sassi, J.F., Lerat, Y., & Lahaye, M. (2009c). Determination of the chemical composition of ulvan, a cell wall polysaccharide from Ulva spp. (Ulvales, Chlorophyta) by FT-IR and chemometrics. Journal of Applied Phycology, 21, 451–456. http://doi:10.1007/s10811-008-9390-9
Santi, R.A., Sunarti, T.C., Santoso, D., & Triwisari, D.A. (2012). Komposisi kimia dan profil polisakarid rumput laut hijau. Jurnal Akuatika, 3(2), 105-114.
Schwanninger, M., Rodrigues, J., Pereira, H., & Hinterstoisser, B. (2004). Effects of short- time vibratory ball milling on the shape of FT-IR spectra of wood and cellulose. Vibrational Spectroscopy, 36, 23–40. https://doi.org/10.1016/j.vibspec.2004.02.003
Sianipar, E.A., Satriawan, N., Sumartono, J., & Kambira, P.F.A. (2022). Pengujian aktivitas antioksidan makro alga Sumbawa dalam hubungannya dengan kandungan senyawa bioaktif dan efek farmakologi. Jurnal Riset Kesehatan Nasional, 6(2), 151-157.
Sihono, Sinurat, E., Fateha, Supriyanto, A., Suryaningrum, T. D., Nurhayati, Fransiska, D., Utomo, B. S. B., Subaryono, Sedayu, B. B., Waryanto, Nurjanah, Ramadhan, W., Fadillah, H. M., & Muzayyanah, A. L. (2023). Optimasi formula nori-like product dari Ulva spp., Gracilaria sp., dan gliserol menggunakan metode mixture design. Jurnal Pengolahan Hasil Perikanan Indonesia, 26(3), 433-447. http://dx.doi.org/10.17844/jphpi.v26i3.48337
Sun, L., Wang, L., Li, J., & Liu, H. (2014). Characterization and antioxidant activities of degraded polysaccharides from two marine chrysophyta. Food Chemistry, 160, 1-7. https://doi.org/10.1016/j.foodchem.2014.03.067
Suresh, V., Senthilkumar, N., Thangam, R., Rajkumarc, M., Anbazhagana, C., Rengasamy, R., Gunasekaranc, P., Kannand, S., & Palanib, P. (2013). Separation, purification and preliminary characterization of sulfated polysaccharides from Sargassum plagiophyllum and its in vitro anticancer and antioxidant activity. Process Biochemistry, 48, 364–373. http://dx.doi.org/10.1016%2Fj.procbio.2012.12.014
Tako, M., Tamanaha, M., Tamashiro, Y., & Uechi, S. (2015). Structure of ulvan isolated from the edible green seaweed, Ulva pertusa. Advances in Bioscience and Biotechnology, 6, 645-655. http://dx.doi.org/10.4236/abb.2015.610068.
Tsubaki, S., Oono, K., Hiraoka, M., Onda, A., & Mitani, T. (2016). Microwave-assisted hydrothermal extraction of sulfated polysaccharides from Ulva spp. and Monostroma latissimum. Food Chemistry, 210, 311–316. https://doi.org/10.1016/j.foodchem.2016.04.121
Valentine, G., Sumardianto, & Wijayanti, I. (2020). Karakteristik nori dari rumput laut Ulva lactuca dan Gelidium sp. Jurnal Pengolahan Hasil Perikanan Indonesia, 23(2): 295-302. https://doi.org/10.17844/jphpi.v23i2.32340
Venkatesan, J., Lowe, B., Anil, S., Manivasagan, P., Kheraif, A.A.A., Kang, K.H., & Kim, S.K. (2015). Seaweed polysaccharides and their potential biomedical application. Starch-Staerke, 67, 381-390. https://doi.org/10.1002/star.201400127
Wahlström, N., Nylander, F., Malmhäll-Bah, E., Sjövold, K., Edlunda, U., Westman, G., & Albers, E. (2020). Composition and structure of cell wall ulvans recovered from Ulva spp. along the Swedish west coast. Carbohydrate Polymers, 233, 1-9. https://doi.org/10.1016/j.carbpol.2020.115852
Weafer, C. (1996). The Food Chemistry Laboratory. CRC Press.
Widowaty, W., Setiawan, S., & Perdana, W.W. (2020). Aktivitas antioksidan ekstrak Gracilaria sp. dan Ulva sp. dari pantai Sayang Heulang. Agroscience, 10(2), 203-209.
Wijesekara, I., Pangestuti, R., & Kim, S.-K. (2011). Biological activities and potential health benefits of sulfated polysaccharides derive from marine algae. Carbohydrate Polymers, 84, 14-21. https://doi.org/10.1016/j.carbpol.2010.10.062
Wu, H.Z., Wang, J.M., Yang, H., Li, G.Q., Zeng, Y.H., Xia, W., Li, Z.G., & Qian, M.R. (2017). Development and application of an in-cell clean up pressurized liquid extraction with ultra-high-performance liquid chromatography-tandem mass spectrometry to detect prohibited antiviral agents sensitively in livesstock and poultry feces. Journal of Chromatography, 1488, 10-16. https://doi.org/10.1016/j.chroma.2017.01.070
Yaich, H., Garna, H., Besbes, S., Paquot, M., Blecker, C., & Attia, H. (2013). Effect of extraction conditions on the yield and purity of ulvan extracted from Ulva lactuca. Food Hydrocolloid, 31(2), 375-382. https://doi.org/10.4025/actascibiolsci.v40i1.34916
Yaich, H., Amira, A.B. , Abbes, F., Bouaziz, M., Besbes, S., Richel, A., Blecker, C., Attia, H., & Garna, H. (2017). Effect of extraction procedures on structural, thermal and antioxidant properties of ulvan from Ulva lactuca collected in Monastir coast. International Journal of Biological Macromolecules, 105(2), 1430-1439. https://doi.org/10.1016/j.ijbiomac.2017.07.141
Authors
JacoebA. M., AbdullahA., & HakimahS. N. (2024). Potensi ulvan dari Ulva lactuca sebagai sumber antioksidan: Potential of ulvan from Ulva lactuca as an antioxidant source. Jurnal Pengolahan Hasil Perikanan Indonesia, 27(3), 242-251. https://doi.org/10.17844/jphpi.v27i3.46950
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