Pengaruh Suhu Ekstraksi terhadap Aktivitas Antioksidan Ekstrak Metanolik Eucheuma spinosum Effect of Extraction Temperature on Antioxidant Activity of Methanolic Extract of Eucheuma spinosum

Komala Putu Tara Hradaya, Amir Husni

Abstract

Eucheuma spinosum is red algae that is widely cultivated and is also one of the superior commodities because it has phenolic and flavonoid compounds that can act as antioxidants. Phenolic content and flavonoids in E. spinosum can be obtained through a maceration process with methanol as a solvent which is generally carried out at room temperature. The extraction process to obtain maximum yield can be accelerated by increasing the extraction temperature. This research aims to determine the antioxidant activity of E. spinosum extracted using methanol at temperatures of 55, 65, and 75 °C. Before extraction, the seaweed was washed and dried in an oven at 40 °C for 24 hours. Extraction was carried out with 80 % methanol in a ratio of 1:10 (w/v). The parameters analyzed in this research were yield, phytochemical test, total phenolic content (TPC), total flavonoids, antioxidant activity including DPPH and FRAP. The data indicated that the extraction temperature affected total phenolic and antioxidant activity of both DPPH and FRAP, but did not affect the yield and total flavonoids. Extraction temperature of 55 °C had higher antioxidant activity with an IC50 DPPH value 362.52±7.10 ppm and FRAP value 70.30±10.01 μM equivalent to Fe (II)/g.

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Authors

Komala Putu Tara Hradaya
Amir Husni
a-husni@ugm.ac.id (Primary Contact)
Author Biography

Amir Husni, Universitas Gadjah Mada

Fisheries 1
Putu Tara HradayaK., & HusniA. (2021). Pengaruh Suhu Ekstraksi terhadap Aktivitas Antioksidan Ekstrak Metanolik Eucheuma spinosum: Effect of Extraction Temperature on Antioxidant Activity of Methanolic Extract of Eucheuma spinosum. Jurnal Pengolahan Hasil Perikanan Indonesia, 24(1), 1-10. https://doi.org/10.17844/jphpi.v24i1.34193

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