Kualitas Mikrobiologi Cacing Laor (Polychaeta) dari Perairan Pantai Lawena, Desa Hutumury Kota Ambon. Micobiologicaly Quality Of “Laor” Worms (Polychaeta) From Lawena Beach, Village Hutumury Ambon City
Article Sidebar
Published
31 August 2020
Keywords:
-
contamination, foos safety, public health, Vibrio sp.
Abstract
“Laor” worms were organisms that originated at the base of the waters, for that reason, sometime it is possible contaminated by pathogenic bacteria. This study aims to determine the quality characteristics of “Laor” worms. This research was using the descriptive method in the form of morphological characterization, and biochemical tests on the isolates of lactic acid bacteria (LAB) and Vibrio sp of “laor” worms. The result showed the total bacteria, LAB, and Vibrio sp contained in fresh “laor” worms were (1.2x104-1.6x104) CFU/g; (6.8x104-7.4x104) CFU/g, and (6.0x103-7.0x103) CFU/g respectively. Morphological and biochemical characterization found that LAB isolates have a rounded colony shape, small size, milky white color with convex elevation, round cell shape, short chain (Isolate Bl 6), short stem cell shape (isolate Bl 2, Bl 3, Bl 4) , the shape of short chain stem cells (isolate Bl 3) and an isolates of Vibrio colonies are rounded shape (isolates Vb1, Vb2, Vb3 and Vb6, Vb4), An isolate of Vb5 has an irregular colony shape, yellow colored with a smooth texture. Interpretation results of catalyses test of the two isolates showed positive and Gram staining of LAB isolates was Gram positive while Vibrio Isolate was Gram negative. This finding can be used as a basis for continued of microbiological testing related to bacterial pathogenicity that isolated from “laor” worms.
References
Adebayo-Tayo AC, Odu NN, Michael MU, Okonko IO. 2012. Multi-drug resistant (MDR) organisms isolated from sea-foods in Uyo, South-Southern Nigeria. Nature and Science. 10(3): 61-70.
Alam MJ, Tomochika KI, Miyoshi SI, Shinoda S. 2002. Environmental investigationof potentially pathogen Vibrio parahaemolyticus in the Seto-Inland Sea, Japan. FEMS Microbiol Letters. 208(1): 83–87.
Baffone W, Pianetti A, Bruscolini F, Barbieri D, Citterio B. 2000: Occurrence and expression of virulence related properties of Vibrio species isolated from widely consumed seafood products. International Journal of Food Microbiology. 54(1): 9–18.
[BSN] Badan Standarisasi Nasional. 2006. Penentuan Angka Lempeng Total (ALT) pada Produk Perikanan -SNI 01-2332.3- 2006. Jakarta (ID): Badan Standardisasi Nasional.
Cappucino JG, Sherman N. 1992. Microbiology, A Laboratory Manual. New York (AS): The Benjamin/Cummings Publishing Company.
Coman GJ, Arnold SJ, Callaghan TR, Preston NP. 2007. Effect of two maturation diet combinations on reproductive performance of domesticated Penaeus monodon. Aquaculture. 263(1): 75-83.
DePaola A, Ulaszek J, Kaysner CA, Tenge BJ, Nordstrom JL,Well J, Puhr N, Gendel SM, 2003. Molecular, serological, and virulence characteristics of Vibrio parahaemolyticus isolated from environmental, food, and clinical sources in North America and Asia. Applied Environmental Microbiolgy. 69(7): 3999–4005
[EEC] European Economic Community. 1992. Opinion of the scientific committee on veterinary measures relating to public health on Vibrio vulnificus and Vibrio parahaemolyticus (in raw and undercooked seafood).
Hermawan D, Saifullah, Herdiyana D. 2015, Pengaruh Perbedaan Jenis Substrat pada Pemeliharaan Cacing Laut (Nereis sp.) (The Effect of Different Substrat of Culture of Nereis sp.). Jurnal Perikanan dan Kelautan. 5(1): 41-47
Ignatius B. 2001. Spawning and larval rearingtechnique for tropical clownfish Amphiprion sebae under captive condition. Journal aquaculture Tropical. 16(3): 241-249.
Jaksic S, Uhitil S, Petrak T, Bazulic D, Karolyi LG. 2002. Occurrence of Vibrio spp. in sea fish, shrimps and bivalve molluscs harvested from Adriatic sea. Food Control. 13(8): 491-493.
Junardi, Wardoyo ERP. 2008. Struktur komunitas dan karakteristik substrat cacing laut (Polychaeta) di Perairan Pantai Mangrove Peniti, Kalimantan Barat. BIODIVERSITAS. 9(3): 213-216
Latumahina MChA. 2011. Pengolahan Dan Komposisi Gizi Cacing Polychaeta Di Pulau Ambon. [Prosiding Seminar Nasional]: Pengembangan Pulau-Pulau Kecil. Bogor (ID) Institut Pertanian Bogor.
Latumahina MChA, Mailoa MN. 2016. Iodine content and nutrition worms Polychaeta “laor” fresh and processed products. International Journal of ChemTech Research. 9(1): 147-150
Lay BW. 1994. Analisa Mikroba di Laboratorium. Jakarta (ID): Raja Grafindo Persada.
Liline S. 2017. Analisis kadar protein cacing laor (polychaeta) dari perairan pulau ambon. Biopendix. 3(2): 167-171
Pal M. 2012. Food spoilage. Ph.D. Lecture Notes. Addis Ababa University,College of Veterinary Medicine. Ethiopia (ET) : Debre Zeit.
Pal M. 2007. Zoonoses.2nd Ed. Jaipur India. India (IND): Satyam Publishers.
Pamungkas J. 2009. Pengamatan jenis cacing laor (annelida, Polychaeta) di perairan desa latuhalat Pulau ambon, dan aspek reproduksinya. Jurnal TRITON. 5(2):1-10
Pramono H, Noor HM, Fatimah SS, Harahap NA, Selia AA. 2015.isolasi dan identifikasi vibrio sp. Pada produk seafood tradisional area timur kota surabaya. Jurnal Ilmiah Perikanan dan Kelautan. 7(1) : 25-29
Radjawane TR.1982. Laor: Cacing Laut Khas Perairan Maluku, Lomba Karya Penelitian Ilmiah Remaja, Jakarta (ID): Departemen Pendidikan dan Kebudayaan Republik Indonesia.
Reilly A. 2006. Managing Microbiological Risk in the Fish Processing Chain. In: FAO EUROFISH Workshop on Seafood Safety, Copenhagen, December 13-15, 2006.
Rhea F. 2009. Microbiology handbook: Fish and seafood. Leatherhead Food. UK (US) : International Ltd. Surrey.
Sari R, Apridamayanti P. 2014 . Cemaran bakteri Eschericia coli dalam beberapa makanan laut yang beredar di pasar tradisional kota pontianak. Kartika Jurnal Ilmiah Farmasi. 2 (2): 14-19
Savitri IKE, Latumahina M, Tapotubun AM. 2008. Studi Kandungan Gizi Laor. [Prosiding Seminar Nasional Perikanan dan Kelautan], Malang (ID): Fakultas Perikanan dan Kelautan Universitas Brawijaya.
Su YC, Liu C. 2007. V. parahaemolyticus: a concernof seafood safety. Food Microbiology. 24 (6): 549–558
Tampubolon K, Purnomo D, Sangadji M. 2007. Pengolahan pasta laor (Eunice viridis) dengan berbagai konsentrasi garam. Buletin Teknologi Hasil Perikanan. 10 (1):47-58.
Tuyet DT, Thiem VD, Von Seidlein L, Chowdhury A, Park E, Canh DG, Chien BT,Van Tung T, Naficy A, Rao MR, Ali M, Lee H, Sy TH, Nichibuchi M, Clemens J, Trach DD. 2002. Clinical, epidemiological, and socioeconomic analysis of anoutbreak of Vibrio parahaemolyticus in Khanh Hoa Province, Vietnam. The Journal Infectious Diseases.186: 1615–1620
Widianwari P dan Widianingsih, 2011. Komunitas Cacing Laut Dalam (Polychaeta) di Selat Flores, Lamakera dan Alor, Nusa Tenggara Timur. Ilmu Kelautan. 16 (4): 219-228
Yang, ZQ, Jiao XA, Zhou XH, Cao GX, Fang WM, Gu RX.2008. Isolation and molecular characterization of Vibrio parahaemolyticus from fresh, low-temperature preserved, dried, and salted seafood products in two coastal areas of eastern China. International Journal of Food Microbiology. 125(3):279-285.
Alam MJ, Tomochika KI, Miyoshi SI, Shinoda S. 2002. Environmental investigationof potentially pathogen Vibrio parahaemolyticus in the Seto-Inland Sea, Japan. FEMS Microbiol Letters. 208(1): 83–87.
Baffone W, Pianetti A, Bruscolini F, Barbieri D, Citterio B. 2000: Occurrence and expression of virulence related properties of Vibrio species isolated from widely consumed seafood products. International Journal of Food Microbiology. 54(1): 9–18.
[BSN] Badan Standarisasi Nasional. 2006. Penentuan Angka Lempeng Total (ALT) pada Produk Perikanan -SNI 01-2332.3- 2006. Jakarta (ID): Badan Standardisasi Nasional.
Cappucino JG, Sherman N. 1992. Microbiology, A Laboratory Manual. New York (AS): The Benjamin/Cummings Publishing Company.
Coman GJ, Arnold SJ, Callaghan TR, Preston NP. 2007. Effect of two maturation diet combinations on reproductive performance of domesticated Penaeus monodon. Aquaculture. 263(1): 75-83.
DePaola A, Ulaszek J, Kaysner CA, Tenge BJ, Nordstrom JL,Well J, Puhr N, Gendel SM, 2003. Molecular, serological, and virulence characteristics of Vibrio parahaemolyticus isolated from environmental, food, and clinical sources in North America and Asia. Applied Environmental Microbiolgy. 69(7): 3999–4005
[EEC] European Economic Community. 1992. Opinion of the scientific committee on veterinary measures relating to public health on Vibrio vulnificus and Vibrio parahaemolyticus (in raw and undercooked seafood).
Hermawan D, Saifullah, Herdiyana D. 2015, Pengaruh Perbedaan Jenis Substrat pada Pemeliharaan Cacing Laut (Nereis sp.) (The Effect of Different Substrat of Culture of Nereis sp.). Jurnal Perikanan dan Kelautan. 5(1): 41-47
Ignatius B. 2001. Spawning and larval rearingtechnique for tropical clownfish Amphiprion sebae under captive condition. Journal aquaculture Tropical. 16(3): 241-249.
Jaksic S, Uhitil S, Petrak T, Bazulic D, Karolyi LG. 2002. Occurrence of Vibrio spp. in sea fish, shrimps and bivalve molluscs harvested from Adriatic sea. Food Control. 13(8): 491-493.
Junardi, Wardoyo ERP. 2008. Struktur komunitas dan karakteristik substrat cacing laut (Polychaeta) di Perairan Pantai Mangrove Peniti, Kalimantan Barat. BIODIVERSITAS. 9(3): 213-216
Latumahina MChA. 2011. Pengolahan Dan Komposisi Gizi Cacing Polychaeta Di Pulau Ambon. [Prosiding Seminar Nasional]: Pengembangan Pulau-Pulau Kecil. Bogor (ID) Institut Pertanian Bogor.
Latumahina MChA, Mailoa MN. 2016. Iodine content and nutrition worms Polychaeta “laor” fresh and processed products. International Journal of ChemTech Research. 9(1): 147-150
Lay BW. 1994. Analisa Mikroba di Laboratorium. Jakarta (ID): Raja Grafindo Persada.
Liline S. 2017. Analisis kadar protein cacing laor (polychaeta) dari perairan pulau ambon. Biopendix. 3(2): 167-171
Pal M. 2012. Food spoilage. Ph.D. Lecture Notes. Addis Ababa University,College of Veterinary Medicine. Ethiopia (ET) : Debre Zeit.
Pal M. 2007. Zoonoses.2nd Ed. Jaipur India. India (IND): Satyam Publishers.
Pamungkas J. 2009. Pengamatan jenis cacing laor (annelida, Polychaeta) di perairan desa latuhalat Pulau ambon, dan aspek reproduksinya. Jurnal TRITON. 5(2):1-10
Pramono H, Noor HM, Fatimah SS, Harahap NA, Selia AA. 2015.isolasi dan identifikasi vibrio sp. Pada produk seafood tradisional area timur kota surabaya. Jurnal Ilmiah Perikanan dan Kelautan. 7(1) : 25-29
Radjawane TR.1982. Laor: Cacing Laut Khas Perairan Maluku, Lomba Karya Penelitian Ilmiah Remaja, Jakarta (ID): Departemen Pendidikan dan Kebudayaan Republik Indonesia.
Reilly A. 2006. Managing Microbiological Risk in the Fish Processing Chain. In: FAO EUROFISH Workshop on Seafood Safety, Copenhagen, December 13-15, 2006.
Rhea F. 2009. Microbiology handbook: Fish and seafood. Leatherhead Food. UK (US) : International Ltd. Surrey.
Sari R, Apridamayanti P. 2014 . Cemaran bakteri Eschericia coli dalam beberapa makanan laut yang beredar di pasar tradisional kota pontianak. Kartika Jurnal Ilmiah Farmasi. 2 (2): 14-19
Savitri IKE, Latumahina M, Tapotubun AM. 2008. Studi Kandungan Gizi Laor. [Prosiding Seminar Nasional Perikanan dan Kelautan], Malang (ID): Fakultas Perikanan dan Kelautan Universitas Brawijaya.
Su YC, Liu C. 2007. V. parahaemolyticus: a concernof seafood safety. Food Microbiology. 24 (6): 549–558
Tampubolon K, Purnomo D, Sangadji M. 2007. Pengolahan pasta laor (Eunice viridis) dengan berbagai konsentrasi garam. Buletin Teknologi Hasil Perikanan. 10 (1):47-58.
Tuyet DT, Thiem VD, Von Seidlein L, Chowdhury A, Park E, Canh DG, Chien BT,Van Tung T, Naficy A, Rao MR, Ali M, Lee H, Sy TH, Nichibuchi M, Clemens J, Trach DD. 2002. Clinical, epidemiological, and socioeconomic analysis of anoutbreak of Vibrio parahaemolyticus in Khanh Hoa Province, Vietnam. The Journal Infectious Diseases.186: 1615–1620
Widianwari P dan Widianingsih, 2011. Komunitas Cacing Laut Dalam (Polychaeta) di Selat Flores, Lamakera dan Alor, Nusa Tenggara Timur. Ilmu Kelautan. 16 (4): 219-228
Yang, ZQ, Jiao XA, Zhou XH, Cao GX, Fang WM, Gu RX.2008. Isolation and molecular characterization of Vibrio parahaemolyticus from fresh, low-temperature preserved, dried, and salted seafood products in two coastal areas of eastern China. International Journal of Food Microbiology. 125(3):279-285.
Authors
Nelce MailoaM., Bonan Dolok SorminR., & LewaherillaJ. (2020). Kualitas Mikrobiologi Cacing Laor (Polychaeta) dari Perairan Pantai Lawena, Desa Hutumury Kota Ambon.: Micobiologicaly Quality Of “Laor” Worms (Polychaeta) From Lawena Beach, Village Hutumury Ambon City. Jurnal Pengolahan Hasil Perikanan Indonesia, 23(2), 352-358. https://doi.org/10.17844/jphpi.v23i2.32366
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Article Details
