Karakteristik fisikokimia kolagen larut asam dari kulit ikan parang-parang (Chirocentrus dorab)
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Accepted
31 December 2019
Published
31 December 2019
Keywords:
-
collagen isolation, FTIR, viscosity
Abstract
Waste of parang parang fish (Chirocentrus dorab) skin can be used as a source of collagen. Collagen isolation can be done chemically by the Acid Soluble Collagen (ASC) method. The objective of this research was to isolate collagen with ASC method and characterize their physicochemical. Collagen isolation consisted of pretreatment and hydrolysis with acids. The pretreatment used NaOH 0.1 M for 12 hours, while hydrolysis used acetic acid 0.5 M. Pretreatment results indicated that the concentration of non-collagen protein was 0.1243 mg/mL, while the yield collagen was 2.61%. The collagen had the viscosity of 6.50 cP, the denaturation temperature of 4°C, the transition temperature of 77.30°C, and the melting temperature of 153.90°C. The obtained collagen also had pH of 6.25. The fourier transform infrared (FTIR) spectra analysis showed the collagen contained amide A (3425.58), B (2924.09), I (1647.21), II (1543.05), and III (1246.02) (cm-1). The collagen also contained glycine (26.69%), proline (12.24%) and alanine (9.51%).
References
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Aberoumand A. 2012. Comparative study between different methods of collagen extraction from fish and its properties. World Applied Science Journal. 16 (3): 316-319
Ahmad M, Benjakul S. 2010. Extraction and characterisation of pepsin solubilised collagen from the skin of unicorn leatherjacket (Aluterus monocerous). Food Chemistry. 120:817-824
Alhana. 2015. Ekstraksi dan karakterisasi kolagen dan nanokolagen dari daging teripang gamma (Stichopus variegatus) [Tesis]. Bogor(ID): Institut Pertanian Bogor
Astiana I. 2016. Efektivitas asam dan enzim papain dalam menghasilkan kolagen dari kulit ikan ekor kuning (Caesio cuning) [Tesis]. Bogor (ID): Institut Pertanian Bogor
Barth A. 2007. Review: Infrared spectroscopy of proteins. Biochimica et Biophysca Acta. 1767: 1073-1101
Bradford MM. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analitycal Biochemistry. 72(1): 248-254
Chi CF, Cao ZH, Wang B, Hu FY, Li ZR, Zhang B. 2014. Antioxidant and functional propeties of collagen hidrolysates from spanis mackerel as influenced by average molecular weight. Molecules. 19: 11211-11230.
Chi CF, Wang B, Li ZR, Luo HY, Ding GF. 2013. Characterization of acid-soluble collagens from the cartilages of scalloped hammerhead (Sphyrna lewini), red stingray (Dasyatis akajei), and skate (Raja porosa). Food Science and Biotechnology. 22: 909-9016
Coates J. 2000. Interpreration of infrared spectra, a practical approach.: Meyers RA, editor. Encyclopedia of Analytical Chemistry. USA (US): John Wiley & Sons Ltd.
Erizal, Abbas B, Setyo AK, Sulistiono GS, Sudirman. 2014. Pengaruh iradiasi gamma pada sifat fisiko-kimia kolagen dalam larutan. Jurnal Sains Materi Indonesia. 15(4): 221-225.
Faisal F. 2014. Isolasi dan karakterisasi kolagen dari kulit ikan buntal (Tetraodon lunaris) [Skripsi]. Bogor(ID): Institut Pertanian Bogor.
Fawzya YN, Chasanah E, Poernomo A, Khirzin MH. 2016. Isolasi dan karakterisasi parsial kolagen dari teripang gamma (Sticopus variegatus). Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 11(1): 91-100
Goissis G, Piccirili L, Goes JC, Plepis AM, Das-Gupta DK. 1998. Anionik collagen: polimer composites with improved dielectric and rheological properties. Artificial Organs. 22: 203-209.
Hidayat AS, Siradj M. 2015. Sertifikasi halal dan sertifikasi non halal pada produk pangan industri. Ahkam: Jurnal Ilmu Syariah. 15:199-222
Huang YR, Shiau CY, Chen HH, Huang BC. 2011. Isolation and characterization of acid and pepsin-solubilized collagens from the skin of balloon fish (Diodon holocanthus). Food Hydrocolloids. 25:1507-1513.
Jamilah B, Hartina UMR, Hashim MD, Sazili AQ. 2013. Properties of collage from Barramundi (Lates calcarifer) skin. International Food Research Journal. 20(2): 835-842.Jaswir I, Monsur HA, Salleh HM. 2011. Nano-structural analysis of fish collagen extracts for new process development. African Journal of Biotechnology. 10(81):18847-18854.
Kittiphattanabawon P, Benjakul S, Visessanguan W, Shahidi F. 2010. Isolation and characterization of collagen from the cartilages of brownbanded bamboo shark (Chiloscyllium punctatum) and blacktip shark (Carcharhinus limbatus). Food Science Technology 43: 792–800
Kong J, Yu S. 2007. Fourier transform infrared spectroscopic analysis of protein secondary structures. Acta Biochimica et Biophysca Sinica. 39(8): 549-559.
Krishnamoorthia J, Ramasamya P, Shanmugama V, Shanmugama A. 2017. Isolation and partial charactization of collagen from outer skin sepi pharasonis (Enrenberg, 1831) from producherry coast. Biochemistry and Biophyshics Report. 10. 9-45.
Kumar MH, Spandana V, Poonam T. 2011. Extraction and determination of collagen peptide and its clinical importance from tilapia fish scales (Oreochromis niloticus). International Research Journal of Pharmacy. 2(10): 97-99.
Kurniawan DR. 2016. Formulasi sediaan gel pelembab kulit berbasis kolagen kulit ikan parang-parang (Chirocentrus dorab) [Skripsi]. Bogor (ID): Institut Pertanian Bogor
Li Z, Wang B, Chi C, Zhang Q, Gong Y, Tang J, Luo H, Ding G. 2013. Isolation and characterization of acid soluble collagens and pepsin soluble collagens from the skin and bone of Spanish mackerel (Scomberomorous niphonius). Food Hydrocolloids. 31: 103-113
Liu D, Wei G, Li T, Hua J, Lu J, Regenstein JM, Zhou P. 2015. Effects of alkaline pretreatments and acid extraction conditions on the acid-soluble collagen from grass carp (Ctenopharyngodon idella) skin. Food Chemistry. 172:836–843
Mandal A, Sekar S, Seeni MK, Mukherjee A, Sastry TP, Mandal AB. 2014. Fabrication of collagen scaffolds impregnated with sago starch capped silver nanoparticles suitable for biomedical applications and their physicochemical studies. Physical Chemistry Chemical Physics. 16(37): 20175–20183.
Matmaroh K, Benjakul S, Prodpran T, Encarnacion AB, Kishimura H. 2011. Characteristics of acid soluble collagen and pepsin soluble collagen from scale of spotted golden goatfish (Parupeneus heptacanthus). Food Chemistry.129: 1179-1186.
Muyonga JH, Cole CGB, Duodu KG. 2004. Characterisation of acid soluble collagen from skins of young and adult nile perch (Lates niloticus). Food Chemistry. 85: 81-89
Nagai T, Yamashita E, Taniguchi K, Kanamori N, Suzuki N. 2001. Isolation and characterization of collagen from the outer skin waste material of cuttlefish (Sepia lycidas). Food Chemistry. 72: 425-429
Nur’aenah N. 2013. Ekstraksi dan karakterisasi kolagen dan nanopartikel kolagen dari kulit ikan pari (Pastinachus solocirostris) sebagai bahan baku kosmetik. [Tesis]. Bogor(ID): Institut Pertanian Bogor.
Ogawa M, Portier JR, Moody MW, Bell J, Schexnayder MA, Losso JN. 2004. Biochemical properties of bone and scale collagens isolated from the subtropical fish black drum (Pogonia cromis) and sheepshead sea bream (Archosargus probatocephalus). Food Chemistry. 88: 495–501
Potaros T, Raksakulthai N, Runglerdkreangkrai J, Worawattanamateekul W. 2009. Characteristics of collagen from nile tilapia (Oreochromis niloticus) skin isolated by two different methods. Kasetsart Journal-Natural Science. 43(3): 584-593.
Putra ABN, Sahubawa L, Ekantari N. 2013. Ekstraksi dan Karakterisasi Kolagen dari Kulit Ikan Nila Hitam (Oreochromis niloticus). Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 8(2): 171–180
Ratnasari I, Yuwono SS, Nusyam H, Widjanarko SB. 2013. Extraction and characterization of gelatin from different fresh water fishes as alternative sources of gelatin. International Food Research Journal. 20(6): 3085-3091.
Rengi, P dan Tang, UM. 2013. Identifikasi, Eksplorasi Potensi Dan Sumberdaya Perikanan Tangkap Kabupaten Bengkalis. http://repository.uri.ac.id.
Schmidt MM, Dornelles RCP, Mello RO, Kubota EH, Mazutti MA, Kempka AP, dan Demiate IM. 2016. Mini Review: Collagen extraction process. International Food Research Journal. 23(3):913-922.
Singh P, Benjakul S, Maqsood S, Kishimura H. 2011. Isolation and characterization of collagen extracted from the skin of striped catfish (Pangasianodon hyphothalmus). Food Chemistry. 124: 97-105.
Suryanti S, Marseno DW, Indrati R, Irianto HE. 2017. Pengaruh jenis asam dalam isolasi gelatin dari kulit ikan nila (Oreochromis niloticus) terhadap karakteristik emulsi. Agritech. 37(4):410-419.
Tamilmozhi S, Veeruraj A, Arumugam M. 2013. Isolation and characterization of acid and pepsin-solubilized collagen from the skin of sailfish (Istiophorus platypterus). International Food Reserarch Journal. 54: 1499-1505.
Tziveleka LA, Ioannou E, Tsiourvas D, Berillis P, Foufa E, Roussis V. 2017. Collagen from the marine sponges Axinella cannabina and Suberites carnosus: isolation and morphological, biochemical, and biophysical characterization. Marine Drugs. 15(6): 1-17.
Wulandari, Suptijah P, Tarman K. 2015. Efektivitas pretreatment alkali dan hidrolisis asam asetat terhadap karakteristik kolagen dari kulit ikan gabus. Jurnal Pengolahan Hasil Perikanan Indonesia. 18(3): 287-302.
Wulandari. 2016. Karakterisasi fisikokimia kolagen yang diisolasi dengan metode hidro-ekstraksi dan stabilisasi nanokolagen kulit ikan gabus (Channa striata) [Tesis]. Bogor (ID): Institut Pertanian Bogor.
Yan M, Li B, Zhao X, RenG, Zhuang Y, Hou H, Zhang X, Chen L, Fan Y. 2008. Characterization of acid soluble collagen from the skin of walleye Pollock (Theragra chalcogramma). Food Chemistry. 107:1581-1586.
Yousefi M, Ariffin F, Huda N. 2017. An alternative source of type I collage based on by-product with higher thermal stability. Food Hidrocolloids. 63: 472-382.
Zang Y, Liu W, Li G, Shi B, Miao Y, Wu X. 2007. Isolation and partial characterization of pepsin-soluble collagen from the skin of grass carp (Ctenopharyngodon idella). Food Chemistry. 103: 906-912.
Zhang F, Wang A, Li Z, He S, Shao L. 2011. Preparation and characterisation of collagen from freshwater fish scales. Food and Nutrition Sci. 2:818- 823.
Zaelani BFD, Safithri M, Tarman K, Isetyaningsih I, Meydia. 2019. Collagen isolation with acid soluble method from the skin of red snapper (Lutjanus sp). IOP Conf. Ser.: Earth Environ. Sci. 241 012033. doi:10.1088/1755-1315/241/1/012033.
Abdillah S, Wijayanti G, Setiawan M, Noor US, Nurilmala M. 2017. In vitro anti-tyrosinase and anti-elastase activity of collagen from sea cucumber (Holothuria leucospilota). African Journal of Biotechnology 16(1): 771-776.
Abdussamad EM, Pillai NGK, Zacharia PU, Jeyabalan K. 2011. Dorab Fishery of Gulf of Mannar Waters and Population Characteristics of The Species Chirocentrus dorab (Forsskal, 1775) and Chirocentrus nudus Swainson, 1839. Indian Journal of Fisheries. 58(1): 19-23.
Aberoumand A. 2012. Comparative study between different methods of collagen extraction from fish and its properties. World Applied Science Journal. 16 (3): 316-319
Ahmad M, Benjakul S. 2010. Extraction and characterisation of pepsin solubilised collagen from the skin of unicorn leatherjacket (Aluterus monocerous). Food Chemistry. 120:817-824
Alhana. 2015. Ekstraksi dan karakterisasi kolagen dan nanokolagen dari daging teripang gamma (Stichopus variegatus) [Tesis]. Bogor(ID): Institut Pertanian Bogor
Astiana I. 2016. Efektivitas asam dan enzim papain dalam menghasilkan kolagen dari kulit ikan ekor kuning (Caesio cuning) [Tesis]. Bogor (ID): Institut Pertanian Bogor
Barth A. 2007. Review: Infrared spectroscopy of proteins. Biochimica et Biophysca Acta. 1767: 1073-1101
Bradford MM. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analitycal Biochemistry. 72(1): 248-254
Chi CF, Cao ZH, Wang B, Hu FY, Li ZR, Zhang B. 2014. Antioxidant and functional propeties of collagen hidrolysates from spanis mackerel as influenced by average molecular weight. Molecules. 19: 11211-11230.
Chi CF, Wang B, Li ZR, Luo HY, Ding GF. 2013. Characterization of acid-soluble collagens from the cartilages of scalloped hammerhead (Sphyrna lewini), red stingray (Dasyatis akajei), and skate (Raja porosa). Food Science and Biotechnology. 22: 909-9016
Coates J. 2000. Interpreration of infrared spectra, a practical approach.: Meyers RA, editor. Encyclopedia of Analytical Chemistry. USA (US): John Wiley & Sons Ltd.
Erizal, Abbas B, Setyo AK, Sulistiono GS, Sudirman. 2014. Pengaruh iradiasi gamma pada sifat fisiko-kimia kolagen dalam larutan. Jurnal Sains Materi Indonesia. 15(4): 221-225.
Faisal F. 2014. Isolasi dan karakterisasi kolagen dari kulit ikan buntal (Tetraodon lunaris) [Skripsi]. Bogor(ID): Institut Pertanian Bogor.
Fawzya YN, Chasanah E, Poernomo A, Khirzin MH. 2016. Isolasi dan karakterisasi parsial kolagen dari teripang gamma (Sticopus variegatus). Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 11(1): 91-100
Goissis G, Piccirili L, Goes JC, Plepis AM, Das-Gupta DK. 1998. Anionik collagen: polimer composites with improved dielectric and rheological properties. Artificial Organs. 22: 203-209.
Hidayat AS, Siradj M. 2015. Sertifikasi halal dan sertifikasi non halal pada produk pangan industri. Ahkam: Jurnal Ilmu Syariah. 15:199-222
Huang YR, Shiau CY, Chen HH, Huang BC. 2011. Isolation and characterization of acid and pepsin-solubilized collagens from the skin of balloon fish (Diodon holocanthus). Food Hydrocolloids. 25:1507-1513.
Jamilah B, Hartina UMR, Hashim MD, Sazili AQ. 2013. Properties of collage from Barramundi (Lates calcarifer) skin. International Food Research Journal. 20(2): 835-842.Jaswir I, Monsur HA, Salleh HM. 2011. Nano-structural analysis of fish collagen extracts for new process development. African Journal of Biotechnology. 10(81):18847-18854.
Kittiphattanabawon P, Benjakul S, Visessanguan W, Shahidi F. 2010. Isolation and characterization of collagen from the cartilages of brownbanded bamboo shark (Chiloscyllium punctatum) and blacktip shark (Carcharhinus limbatus). Food Science Technology 43: 792–800
Kong J, Yu S. 2007. Fourier transform infrared spectroscopic analysis of protein secondary structures. Acta Biochimica et Biophysca Sinica. 39(8): 549-559.
Krishnamoorthia J, Ramasamya P, Shanmugama V, Shanmugama A. 2017. Isolation and partial charactization of collagen from outer skin sepi pharasonis (Enrenberg, 1831) from producherry coast. Biochemistry and Biophyshics Report. 10. 9-45.
Kumar MH, Spandana V, Poonam T. 2011. Extraction and determination of collagen peptide and its clinical importance from tilapia fish scales (Oreochromis niloticus). International Research Journal of Pharmacy. 2(10): 97-99.
Kurniawan DR. 2016. Formulasi sediaan gel pelembab kulit berbasis kolagen kulit ikan parang-parang (Chirocentrus dorab) [Skripsi]. Bogor (ID): Institut Pertanian Bogor
Li Z, Wang B, Chi C, Zhang Q, Gong Y, Tang J, Luo H, Ding G. 2013. Isolation and characterization of acid soluble collagens and pepsin soluble collagens from the skin and bone of Spanish mackerel (Scomberomorous niphonius). Food Hydrocolloids. 31: 103-113
Liu D, Wei G, Li T, Hua J, Lu J, Regenstein JM, Zhou P. 2015. Effects of alkaline pretreatments and acid extraction conditions on the acid-soluble collagen from grass carp (Ctenopharyngodon idella) skin. Food Chemistry. 172:836–843
Mandal A, Sekar S, Seeni MK, Mukherjee A, Sastry TP, Mandal AB. 2014. Fabrication of collagen scaffolds impregnated with sago starch capped silver nanoparticles suitable for biomedical applications and their physicochemical studies. Physical Chemistry Chemical Physics. 16(37): 20175–20183.
Matmaroh K, Benjakul S, Prodpran T, Encarnacion AB, Kishimura H. 2011. Characteristics of acid soluble collagen and pepsin soluble collagen from scale of spotted golden goatfish (Parupeneus heptacanthus). Food Chemistry.129: 1179-1186.
Muyonga JH, Cole CGB, Duodu KG. 2004. Characterisation of acid soluble collagen from skins of young and adult nile perch (Lates niloticus). Food Chemistry. 85: 81-89
Nagai T, Yamashita E, Taniguchi K, Kanamori N, Suzuki N. 2001. Isolation and characterization of collagen from the outer skin waste material of cuttlefish (Sepia lycidas). Food Chemistry. 72: 425-429
Nur’aenah N. 2013. Ekstraksi dan karakterisasi kolagen dan nanopartikel kolagen dari kulit ikan pari (Pastinachus solocirostris) sebagai bahan baku kosmetik. [Tesis]. Bogor(ID): Institut Pertanian Bogor.
Ogawa M, Portier JR, Moody MW, Bell J, Schexnayder MA, Losso JN. 2004. Biochemical properties of bone and scale collagens isolated from the subtropical fish black drum (Pogonia cromis) and sheepshead sea bream (Archosargus probatocephalus). Food Chemistry. 88: 495–501
Potaros T, Raksakulthai N, Runglerdkreangkrai J, Worawattanamateekul W. 2009. Characteristics of collagen from nile tilapia (Oreochromis niloticus) skin isolated by two different methods. Kasetsart Journal-Natural Science. 43(3): 584-593.
Putra ABN, Sahubawa L, Ekantari N. 2013. Ekstraksi dan Karakterisasi Kolagen dari Kulit Ikan Nila Hitam (Oreochromis niloticus). Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 8(2): 171–180
Ratnasari I, Yuwono SS, Nusyam H, Widjanarko SB. 2013. Extraction and characterization of gelatin from different fresh water fishes as alternative sources of gelatin. International Food Research Journal. 20(6): 3085-3091.
Rengi, P dan Tang, UM. 2013. Identifikasi, Eksplorasi Potensi Dan Sumberdaya Perikanan Tangkap Kabupaten Bengkalis. http://repository.uri.ac.id.
Schmidt MM, Dornelles RCP, Mello RO, Kubota EH, Mazutti MA, Kempka AP, dan Demiate IM. 2016. Mini Review: Collagen extraction process. International Food Research Journal. 23(3):913-922.
Singh P, Benjakul S, Maqsood S, Kishimura H. 2011. Isolation and characterization of collagen extracted from the skin of striped catfish (Pangasianodon hyphothalmus). Food Chemistry. 124: 97-105.
Suryanti S, Marseno DW, Indrati R, Irianto HE. 2017. Pengaruh jenis asam dalam isolasi gelatin dari kulit ikan nila (Oreochromis niloticus) terhadap karakteristik emulsi. Agritech. 37(4):410-419.
Tamilmozhi S, Veeruraj A, Arumugam M. 2013. Isolation and characterization of acid and pepsin-solubilized collagen from the skin of sailfish (Istiophorus platypterus). International Food Reserarch Journal. 54: 1499-1505.
Tziveleka LA, Ioannou E, Tsiourvas D, Berillis P, Foufa E, Roussis V. 2017. Collagen from the marine sponges Axinella cannabina and Suberites carnosus: isolation and morphological, biochemical, and biophysical characterization. Marine Drugs. 15(6): 1-17.
Wulandari, Suptijah P, Tarman K. 2015. Efektivitas pretreatment alkali dan hidrolisis asam asetat terhadap karakteristik kolagen dari kulit ikan gabus. Jurnal Pengolahan Hasil Perikanan Indonesia. 18(3): 287-302.
Wulandari. 2016. Karakterisasi fisikokimia kolagen yang diisolasi dengan metode hidro-ekstraksi dan stabilisasi nanokolagen kulit ikan gabus (Channa striata) [Tesis]. Bogor (ID): Institut Pertanian Bogor.
Yan M, Li B, Zhao X, RenG, Zhuang Y, Hou H, Zhang X, Chen L, Fan Y. 2008. Characterization of acid soluble collagen from the skin of walleye Pollock (Theragra chalcogramma). Food Chemistry. 107:1581-1586.
Yousefi M, Ariffin F, Huda N. 2017. An alternative source of type I collage based on by-product with higher thermal stability. Food Hidrocolloids. 63: 472-382.
Zang Y, Liu W, Li G, Shi B, Miao Y, Wu X. 2007. Isolation and partial characterization of pepsin-soluble collagen from the skin of grass carp (Ctenopharyngodon idella). Food Chemistry. 103: 906-912.
Zhang F, Wang A, Li Z, He S, Shao L. 2011. Preparation and characterisation of collagen from freshwater fish scales. Food and Nutrition Sci. 2:818- 823.
Zaelani BFD, Safithri M, Tarman K, Isetyaningsih I, Meydia. 2019. Collagen isolation with acid soluble method from the skin of red snapper (Lutjanus sp). IOP Conf. Ser.: Earth Environ. Sci. 241 012033. doi:10.1088/1755-1315/241/1/012033.
Authors
SafithriM., TarmanK., SuptijahP., & WidowatiN. (2019). Karakteristik fisikokimia kolagen larut asam dari kulit ikan parang-parang (Chirocentrus dorab). Jurnal Pengolahan Hasil Perikanan Indonesia, 22(3), 441-452. https://doi.org/10.17844/jphpi.v22i3.28924
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