Activity of Trimethylamine-N-Oxide Demethylase (TMAOase) in the Forming of Natural Formaldehyde in Lizardfish (Saurida tumbil)
Abstract
Formaldehyde found in the fish has been occurred naturally through the work of trimethylamine-
N-Oxide demethylase (TMAO)ase enzyme that broke TMAO into formaldehyde and dimethylamine.
Formaldehyde found in fish has been occurred during storage. This reserch was aimed to determine the
TMAOase activity and its role in dimethylamine and formaldehyde formation in Greater lizardfish (Saurida
tumbil), which stored at chilling temperature. Observations were made every 2 days for 16 days of storage.
Analysis performed in formaldehyde content determination, dimethylamine content determination, and
specific activity of TMAOase enzyme. The result of formaldehyde test was increasing during storage, from
0,22±0,00 ppm on day 0 to 7,45±0,46 ppm on day 16. The results of dimethylamine test increased during
storage from 0,93±0,50 ppm at day 0 to 27,26±1,8 ppm on day 16. The results of TMAOase specific activity
in Greater lizardfish increased during storage from 1,15 U/mg on day 0, to 16,47 U/mg on day 16.
N-Oxide demethylase (TMAO)ase enzyme that broke TMAO into formaldehyde and dimethylamine.
Formaldehyde found in fish has been occurred during storage. This reserch was aimed to determine the
TMAOase activity and its role in dimethylamine and formaldehyde formation in Greater lizardfish (Saurida
tumbil), which stored at chilling temperature. Observations were made every 2 days for 16 days of storage.
Analysis performed in formaldehyde content determination, dimethylamine content determination, and
specific activity of TMAOase enzyme. The result of formaldehyde test was increasing during storage, from
0,22±0,00 ppm on day 0 to 7,45±0,46 ppm on day 16. The results of dimethylamine test increased during
storage from 0,93±0,50 ppm at day 0 to 27,26±1,8 ppm on day 16. The results of TMAOase specific activity
in Greater lizardfish increased during storage from 1,15 U/mg on day 0, to 16,47 U/mg on day 16.
Authors
NurainiA., NurhayatiT., & NurilmalaM. (2017). Activity of Trimethylamine-N-Oxide Demethylase (TMAOase) in the Forming of Natural Formaldehyde in Lizardfish (Saurida tumbil). Jurnal Pengolahan Hasil Perikanan Indonesia, 20(3), 549-558. https://doi.org/10.17844/jphpi.v20i3.19811
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