Optimasi Formula Sari Edamame dengan Proses Pasteurisasi Berdasarkan Karakteristik Kimia dan Sensori
Abstract
Edamame is a type of white soybeans from Japan but has been cultivated in Indonesia. It is commonly harvested as immature green soybeans. One of its products is edamame milk. This research is aimed to determine the optimized condition for edamame milk formula in terms of isoflavones, total phenolics, antioxidant activity and sensory characteristics (color, taste, aroma and overall) and to verify the optimized formula at optimum conditions according to the suggested result. The research was conducted in four stages. At 1st stage, pasteurization process at 72⁰C for 15 seconds was selected for edamame milk formula, based on hedonic test result. Optimization at the second step indicated that the optimum formula obtained was 1:6 ratio edamame:water and 0.20% food additive concentration for emulsifier and stabilizer. At the third stage, the verification results showed the product had matched with the prediction values: antioxidant activity at 1.61 mg ascorbic acid/100g, isoflavone content at 41.94 µg/g, total phenolics at 99.92 mg GAE/100 mL and acceptable organoleptic properties (scale 1-7) which gave color at 5.88, taste at 4.69, aroma at 5.36 overall at 5.23. At the four stage, comparison with commercial soybean milks, exhibited that edamame milk had higher content of antioxidant activity, isoflavones and total phenolics.
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References
Abumweis SS, Jones PJH. 2007. Plant sterols: Natural plant components with potential beneficial health effects. International News on fats, oils and related materials. Inform 18: 825-82.
Andayani R, Lisawati Y, Maimunah. 2008. Penentuan aktivitas antioksidan, kadar fenolat total dan likopen pada buah tomat (Solanum lycopersicum L). J Sains Teknologi Farmasi 13(1): 1-9.
Anggraini A, Yunianta. 2015. Pengaruh suhu dan lama hidrolisis enzim papain terhadap sifat kimia, fisik dan organoleptik sari edamame. J Pangan Agroindustri 3(3): 1015-25.
Angkasa D, Sulaeman A. 2012. Pengembangan Mi-numan Fungsional Sumber Serat dan Antioksidan dari Daun Hantap (Sterculia oblongata R. Brown.). [Skripsi]. Bogor: Departemen Gizi Masyarakat. Institut Pertanian Bogor
Astuti S. 2008. Isoflavon Kedelai dan Potensinya Seba-gai Penangkap Radikal Bebas. J Teknol Industri Hasil Pertanian 13(2): 127-36.
Ayustaningwarno F. 2014. Teknologi Pangan; Teori Pratis dan Aplikasi. Graha Ilmu, Yogyakarta. ISBN: 978-602-262-212-3.
Boor KJ. 2001. Fluid dairy product quality and safety: Looking to the future. J Dairy Sci 84: 1-11. DOI: 10.3168/jds.S0022-0302(01)74445-1.
[CAC] Codex Alimentarius Comission. 2004. CAC/ RCP 57-2004: Code Of Hygienic Practise For Milk and Milk Products. FAO and WHO, Rome.
Douglas SA, Gray MJ, Crandall AD, Boor KJ. 2000. Characterization of chocolate milk spoilage patterns. J Food Prot 63: 516-21.
Febriyanti S, dan Yunianta. 2015. Pengaruh konsentrasi karagenan dan rasio sari jahe emprit (Zingiber officinale var. Rubrum) terhadap sifat fisik, kima dan organoleptik jelly drink jahe. J Pangan Agroindustri 3(2): 542-50.
Georgetti SR, Vicentini FTMC, Yokoyama CY, Borin MF, Spadaro ACC, Fonseca MJV. 2009. Enhanced in vitro and in vivo antioxidant activity and mobile-zation of free phenolic compounds of soybean flour fermented with different β-glucosidase-producing fungi. J Appl Microbiol 106: 459-66. DOI: 10.1111/ j.1365-2672.2008.03978.x.
Hadriyono KRP, Kurniawati A. 2011. Karakter Kulit Manggis, Kadar Polifenol dan Potensi Antioksidan Manggis pada Berbagai Umur Buah dan Setelah Buah Dipanen. [Skripsi]. Bogor: Departemen Agro-nomi dan Hortikultura, Institut Pertanian Bogor.
Hasibuan, Arini SD. 2011. Pengaruh Suhu Air Pendi-nginan PLTU Terhadap Kandungan Klorofil Pada Air Sungai Sicanang Belawan. [Skipsi]. Medan: Jurusan Kimia, Universitas Sumatra Utara.
Ismanto SD, Neswati, Azizah.2015. Pengaruh Penam-bahan Effervescent Mix dalam Pembuatan Serbuk Effervescent Daun Pegagan (Centella asiatica, L. Urban). Ulya M, Wulandari NKP, Efendi M, Iswanto H, Editor Prosiding Seminar Agroindustri dan Lokakarya Nasional FKPT-TPI. Peranan Teknologi Pertanian dalam Mewujudkan Kemandirian Maritim, Pangan, dan Energi Berkelanjutan, hal A69-A77. ISBN: 978-602-7998-92-6.
Jun MHY, Fong J, Wan X, Yang CS, Ho CT. 2003. Comparison of antioxidant activities of isoflavones from kudzu root (Puerarua labata Ohwi). J Food Sci Technol 68(6): 2117-22 DOI: 10.1111/j.1365-2621.2003.tb07029.x.
Kao FJ, Su NW, Lee MH. 2004. Effect of Water-to-Bean Ratio on the Contents and Compositions of Isoflavones in Tofu. J agric food chem 52: 2277-81. DOI: 10.1021/jf035410w.
Kawiji, Atmaka W, Oktaviana. 2011. Kajian kadar kurkuminoid, total fenol dan aktivitas antioksidan ekstrak temulawak (Curcuma xanthorriza) pada berbagai teknik pengeringan dan proporsi pelarutan. J Teknologi Hasil Pertanian 4(1): 32-40.
Kim EH, Kim SL, Kim SH, Chung IM. 2012. Compa-rison of isoflavones and anthocyanins in soybean (Glycine max (L.) Merill) seeds of different planting dates. J Agric Food Chem 60: 10196-202. DOI:10. 1021/jf3031259.
Kwan A. 2007. Flavonoids and vascular disease. http:// www.freevas.demon.co.uk/student/flavonoids.html.
Lawless HT. 2013. Quantitative Sensory Analysis: Psychophyics, Models and Intelligent Design. John Wiley & Sons, Ltd, Chichester. ISBN: 978-0-470-67346-1.
Madalena, Heriyanto, Hastuti SP, Leenawaty L. 2007. The effect of heating time to the content of pigments and vitamin A in cassava (Manihot esculenta Crantz) and creara-rubber (Manihot glaziovii Muell. Arg) leaves. Indonesian J Chem 7(1): 105-10.
Meenakshi S, Gnanambigai DM, Mozhi ST, Arumugam M, Balasubramanian T. 2009. Total flavonoid and in vitro antioxidant activity of two seaweeds of ra-meshwaram coast. Global J Pharmacol 3(2): 59-62.
Meilgaard MC, GV Civille BT Carr. 2007. Sensory Evaluation Techniques, 4th edition. CRC Press, Boca Raton, Florida. ISBN 9781482216905.
Molyneux P. 2004. The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 26(2): 211-19.
Mudjajanto ES, Kusuma FR. 2005. Susu Kedelai, Susu Nabati yang Menyehatkan. Agromedia Pustaka, Jakarta. ISBN: 979-3702-71-0.
Pambayun R, Gardjito M, Sudarmadji S, Kuswanto KR. 2007. Kandungan fenol dan sifat antioksidan dari berbagai jenis produk gambir (Uncaria Gambir Roxb). Indonesian J Pharm 18(3): 141-6.
Paixa˜o N, Perestrelo R, Marques, Jose’ C, Camara, Jose’ S. 2007. Relationship between antioxidant capacity and total phenolic content of red rose and white wines. J Agric Food Chem 105: 204-14. DOI: 10.1016/j.foodchem.2007.04.017.
Palupi NS, Wardiani LI, Nurtama B. 2016. Optimasi formula kuah jahe dalam pengembangan wedang tahu sebagai pangan fungsional. J Teknol Industri Pangan 27(1): 95-104. DOI: 10.6066/jtip.2016.27. 1.95.
Picauly P, Talahatu J, Mailoa M. 2015. Pengaruh penambahan air pada pengolahan susu kedelai. Agriteknol 4(1): 8-13.
Pramitasari D, Anadhito RBK, Fauza G. 2010. Penam-bahan ekstrak jahe (Zingiber Officinale Rosc.) dalam pembuatan susu kedelai bubuk instan dengan metode spray drying: komposisi kimia, uji sensoris dan aktivitas antioksidan. J Biofarmasi 9(1): 17-25. DOI: 10.13057/biofar/f090104.
Pujimulyani D, Raharjo S, Marsono Y, Santoso U. 2010. Aktivitas antioksidan dan kadar senyawa fenolik pada kunir putih (curcuma mangga val.) segar dan setelah blanching. Agritech 30(2): 68-74.
Purwoko T, Gandjar I, Pawiroharsono S. 2001. Bio-transformasi isoflavon oleh Rhizopus oryzae UICC 524. J Biological Sci 3(2): 7-12.
Raffa D, Maggio B, Raimondi MV, Plescia F, Daidone G. 2017. Recent discoveries of anticancer flavor-noids. Eur J Med Chem 2017: 1-49. DOI: 10.10 16/j.ejmech.2017.07.034.
Rackis, JJ. 1978. Biochemical Changes in Soybeans: Maturation, Post-Harvest Storage and Processing, and Germination. Pages 34-76 In H.O. Hultin and M. Milner (eds), Post-harvest Biology and Technology. Food and Nutrition Press, Westport. ISBN: 0917678052.
Rosiana NM, Amareta DI. 2016. Karakteristik Yogurt Edamame Hasil Fermentasi Kultur Campuran Bak-teri Asam Laktat Komersial Sebagai Pangan Fung-sional Berbasis Biji-Bijian. Penelitian dan Peng-abdian Masyarakat BOPTN, Jember. ISBN: 978-602-14917-3-7.
Samsu SH. 2003. Membangun Argoindustri Bernuansa Ekspor: Edamame (Vegetable Soybean). Graha Ilmu, Yogyakarta.
Silva FVM, Gibbs PA, Nun˜ez H, Almonacid S, Sim-pson R. Thermal Process Pasteurization. Encyclo-pedia of Food Microbiology, Edition: 2nd edition, Publisher: Academic Press. Editors: Batt CA, Torto-rello ML 3: 577-95 DOI: 10.1016/B978-0-12-384730-0.00404-3.
Soyfoods Association of North America. 2005. Whole Soybean. http://www.soyfoods.org/wp-content/uplo ads/2006/12/whole_soybean. pdf.
Susanti DY. 2008. Efek suhu pengeringan terhadap kandungan fenolik dan kandungan katekin ekstrak daun kering gambir. Rahardjo B, Mawardi M, Hero-dian S, Sutiarso L, Rochdiyanto S, Editor. Prosiding Seminar Nasional Teknik Pertanian. Peran Teknik Pertanian dalam Kedaulatan Pangan dan Energi Hayati Menuju Agroindustri yang Berkelanjutan, hal 1-49. ISBN: 978-979-18918-0-6.
Tsukamoto C, Shimada S, Igita K, Kudou S, Kokubun M, Okubo K, Kitamurat K. 1995. Factors affecting isoflavone content in soybean seeds: changes in isoflavones, saponins, and composition of fatty acids at different temperatures during seed development. J Agric Food Chem 43: 1184-92. DOI: 10. 1021/jf00053a012.
Ukieyanna E. 2012. Aktivitas Antioksidan Kadar Fenolik dan Flavonoid Total Tumbuhan Suruhan. [Skripsi]. Bogor: Departemen Biokimia, Institut Pertanian Bogor.
Wajiiroh E. 2013. Optimasi Pembuatan Minuman Fung-sional Berbasis Ekstrak Daun Kumis Kucing (Orthosiphon Aristatus Bi.Miq) pada Skala Pilot Plant. [Tesis]. Bogor: Sekolah Pascasarjana, Institut Pertanian Bogor.
Wang Q, Ge X, Tian X, Zhang Y, Zhang J, Zhang P. 2013. Soy isoflavone: The multipurpose phytoche-mical (Review). J Biomed Rep 1: 697-701 DOI: 10.3892/br.2013.129.
Williamson G, Manach C. 2005. Bioavailability and bioefficacy of polyphenols in humans. Am J Clin Nutr 81(1): 243S-55S.
Yuan D, Yingni PAN, Yan Chen, Toshio Uno, Shahui Zhang, Yoshihiro Kano, 2008. An improved method for basic hydrolysis of isoflavone malonyl-gluco-sides and qualiy evaluation of Chinese soy mate-rials. Chem Pharm Bull 56(1): 1-6. DOI: 10.1248/ cpb.56.1.
