Identifikasi Pepper vein yellows virus yang Berasosiasi dengan Penyakit Yellow Vein Banding pada Tanaman Mentimun di Bali

  • I Dewa Nyoman Nyana Universitas Udayana
  • M. Tinny Lestariningsih Universitas Udayana
  • Ni Nengah Putri Adnyani Universitas Udayana
  • Gede Suastika Institut Pertanian Bogor
DOI: https://doi.org/10.14692/jfi.12.4.109

Abstrak

Yellowing vein banding disease has been reported infecting cucurbit plants in Bali since 2014. Similar vein banding symptom on chilli pepper was observed previously, and early diagnosis indicated infection of Polerovirus. The objective of this research was to confirm the presence of Polerovirus infection on cucumber plant showing yellow vein banding symptom in Bali. Reverse transcription polymerase chain reaction – based detection method was conducted using specific primer pairs PeVYV-CP-F-BamH1/ PeVYV-CP-R-Pst1followed by sequencing and nucleotide sequence analysis.  Specific DNA fragments of ± 650 bp was successfully amplified from field samples.  Nucleotide sequence analysis showed that the sequence has the highest similarity > 95% with Pepper vein yellow virus (PeVYV) infecting chili pepper from Indonesia (Bali, and Rembang), Japan, and Greece.

Unduh

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Diterbitkan
2016-11-21
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NyanaI. D. N., LestariningsihM. T., AdnyaniN. N. P., & SuastikaG. (2016). Identifikasi Pepper vein yellows virus yang Berasosiasi dengan Penyakit Yellow Vein Banding pada Tanaman Mentimun di Bali. Jurnal Fitopatologi Indonesia, 12(4), 109. https://doi.org/10.14692/jfi.12.4.109
Terbitan
Vol 12 No 4 (2016)
Bagian
Articles

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