Deteksi dan Identifikasi Virus pada Umbi Bawang Merah
Abstract
Shallot is an important horticultural crop in Indonesia. One of it’s production constraint is viral disease. Dispersal of viruses on shallots may occurre through shallots bulb trading. However, there is not much information regarding viruses infecting shallot bulbs. Laboratory study was conducted to detect and identify viruses associated with shallot. Random sampling was done for several varieties of shallots (Jawa, Biru, Nganjuk, and Brebes varieties) collected from Bantul Districs. Virus detection and identification was based on RT-PCR using specific primer of OYDV, SYSV, SLV, and ShVX followed by sequencing of the nucleotides. RT-PCR was only successfully amplified Shallot yellow stripe virus (SYSV) with size ~749 bp, while other viruses were not detected. Disease incidence of SYSV on Java and Brebes varieties were 60% and 53.3% respectively. Nucleotide sequences of SYSV CP gene showed the highest homology to SYSV from China and South Korea.
Key words: RT-PCR, sequencing nucleotides, Shallot yellow stripe virus
Downloads
Authors who publish in Jurnal Fitopatologi Indonesia agree to the following terms:
1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository), with an acknowledgement of its initial publication in this journal.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
