Aktivitas Anticendawan Ekstrak Kecombrang Terhadap Colletotrichum acutatum pada Cabai Rawit
Antifungal Activity of Torch Ginger Extract Against Colletotrichum acutatum on Cayenne Pepper
Abstract
Antifungal Activity of Torch Ginger Extract Against Colletotrichum acutatum on Cayenne Pepper
Torch ginger (Etlingera elatior) is a traditional spice plant that has been widely utilize in Indonesia as an antimicrobial in medical. However, the potential of torch ginger as botanical fungicide in phytopathology needs further study. The research aimed to study the antifungal activity of rhizome and leaf extracts of torch ginger using methanol and ethanol solvents against Colletotrichum acutatum. The extracts was tested using in vitro medium poisoning method at five concentration levels with observation parameter including colony diameter, sporulation, and germination of conidium. The in vivo test was conducted on cayenne pepper fruits with observation parameter such as incubation period, disease intensity and treatment efficacy level. The in vitro study showed that among concentration tested, the torch ginger leaf extract in methanol solvent with a concentration of 2.5% as the best concentration which is inhibits C. acutatum growth, sporulation and germination of conidium by effectiveness up to 66.2%, 99.8% and 100%, respectively in compared to other treatments. The in vivo test on cayenne pepper fruits, the methanol leaf extract at concentration 2.5% inhibits the development of C. acutatum growth, while at concentration 4.8% showed able to prolonged incubation period, reduced disease incidence and severity significantly with effectiveness 88.4% and 96%, respectively in compared to control and other treatments. The antifungal activity of methanol leaf extract of torch ginger against C. acutatum due to its highly phenolic compound. This research successfully demonstrated the potential of extract of torch ginger leaf as botanical fungicide to suppress C. acutatum infection, especially on post-harvest cayenne pepper fruits.
Downloads
References
Ahmad AR, Juwita, Ratulangi SAD, Malik A. 2015. Penetapan kadar fenolik dan flavonoid total ekstrak metanol buah dan daun patikala (Etlingera elatior (Jack) R.M.SM). Journal of Pharmaceutical Sciences and Research. 2(1):1–10. DOI: https://doi.org/10.7454/psr.v2i1.3481.
Amanah I, Aznam N. 2016. Penentuan kadar total fenol dan uji aktivitas antioksidan kombinasi ekstrak sarang semut (Myrmecodia pendens Merr. & L.M. Perry) dan ekstrak kencur (Kaempferia galanga Linn.) dengan metode β-carotene bleaching. Jurnal Elemen Kimia. 5(2):1–9.
Anggreini S, Efri, Nurdin M. 2016. Pengaruh tingkat konsentrasi fraksi ekstrak daun mengkudu dan mimba terhadap pertumbuhan dan sporulasi Colletotrichum acutatum. Jurnal Agrotek Tropika. 4(2):43–48. DOI: https://doi.org/10.23960/jat.v4i1.1899.
Anisah K. 2014. Analisa komponen kimia dan uji antibakteri asap cair tempurung kelapa sawit (Elaeis guineensis Jacq.) pada bakteri Staphylococcus aureus 15 dan Pseudomonas aeruginosa [skripsi]. Jakarta (ID): UIN Syarif Hidayatullah.
Cikita I, Hasibuan IH, Hasibuan R. 2016. Pemanfaatan flavonoid ekstrak daun katuk (Sauropus androgynus (L) Merr) sebagai antioksidan pada minyak kelapa. Jurnal Teknik Kimia USU. 5(1):45–51. DOI: https://doi.org/10.32734/jtk.v5i1.1524.
Gunnell PS, Gubler WD. 1992. Taxonomy and morphology of Colletotrichum species pathogenic to strawberry. Mycologia. 84(2):157–165. DOI: https://doi.org/10.1080/00275514.1992.12026122.
Hakim AR. 2009. Uji potensi antifungi ekstrak etanol rimpang kecombrang (Nicolaia speiosa Horan) terhadap Trichophyton mentagrophytes dan Trichophyton rubrum [skripsi]. Jakarta (ID): UIN Syarif Hidayatullah.
Hernawan DE, Setiawan AD. 2003. Review: Ellagitanin; biosintesis, isolasi, dan aktivitas Biologi. Biofarmasi. 1(1):25–38. DOI: https://doi.org/10.13057/biofar/f010105.
Hudaya A, Radiastuti N, Sukandar D, Djajanegara I. 2014. Uji aktivitas antibakteri ekstrak air bunga kecombrang terhadap bakteri Escherichia coli dan Staphylococcus aureus sebagai bahan pangan fungsional. Al-Kauniyah Jurnal Biologi. 7(1):9–15.
Jayawardana HARK, Weerahewa HLD, Saparamadu MDJS. 2015. Enhanced resistance to anthracnose disease in chilli pepper (Capsicum annuum L.) by amendment of the nutrient solution with silicon. Journal of Horticultural Science and Biotechnology. 90(5):557–562. DOI: https://doi.org/10.1080/14620316.2015.11668714.
Kumar S, Kaushik N. 2013. Endophytic fungi isolated from oil-seed crop Jatropha curcas produces oil and exhibit antifungal activity. PLoS One. 8(2):e56202. DOI: https://doi.org/10.1371/journal.pone.0056202.
Luliana S, Nera UP, Kris NM. 2016. Pengaruh cara pengeringan simplisia daun senggani (Melastoma malabathricum L.) terhadap aktivitas antioksidan menggunakan metode DPPH (2,2-difenil-1pikrilhidrazil). Pharmaceutical Sciences and Research. 3(3):120–129. DOI: https://doi.org/10.7454/psr.v3i3.3291.
Nobosse P, Fombang EN, Mbofung CM. 2018. Effects of age and extraction solvent on phytochemical content and antioxidant activity of fresh Moringa oleifera L. leaves. Food Science and Nutrition. 6(8):2188–2198. DOI: https://doi.org/10.1002/fsn3.783.
Oktarina D, Sumpono, Elvia R. 2017. Uji efektivitas asap cair cangkang buah Hevea braziliensis terhadap aktivitas bakteri Escherichia coli. Alotrop. 1(1):1–5. DOI: https://doi.org/10.33369/atp.v1i1.2704.
Palupi H, Yulianah I, Respatijarti. 2015. Uji ketahanan 14 galur cabai besar (Capsicum annuum L.) terhadap penyakit antraknosa (Colletotrichum spp.) dan layu bakteri (Ralstonia solanacearum). Jurnal Produksi Tanaman. 3(8):640–648.
Pradana ME, Devi M, Soekopitojo S. 2023. Pengaruh rasio bunga kecombrang (Etlingera elatior) dan jahe (Zingiber officinale) terhadap mutu kimia minuman fungsional wedang kecombrang. Journal of Food Technology and Agroindustry. 5(1):51–59. DOI: https://doi.org/10.24929/jfta.v5i1.2401.
Punnawich Y, Issarakraisila M, Intana W, Kan C. 2009. Antifungal effects of thai medichinal plants against Colletotrichum gloeosporioides Penz. Philippine Agricultural Scientist. 92(3):265–270.
Ramayani SL, Octaviana RW, Asokawati SS. 2021. Pengaruh perbedaan pelarut terhadap kadar total fenolik dan kadar total flavonoid ekstrak daun kitolod (Isotoma longiflora (L.). Jurnal Akademi Farmasi Prayoga. 6(2):1–10.
Ratnah ST, Salasa AM, Ibrahim I. 2018. Uji potensi antimikroba hasil fraksinasi ekstrak daun kecombrang (Etlingera Elatior) terhadap Candida albicans penyebab keputihan pada ibu hamil. Media Farmasi. 14(2):45–50. DOI: https://doi.org/10.32382/mf.v14i2.595.
Saranani S, Himaniarwati H, Yuliastri WO, Isrul M, Agusmin A. 2021. Studi etnomedisin tanaman berkhasiat obat hipertensi di Kecamatan Poleang Tenggara Kabupaten Bombana Sulawesi Tenggara. Jurnal Mandala Pharmacon Indonesia. 7(1):60–82. DOI: https://doi.org/10.35311/jmpi.v7i1.72.
Saryanah NA, Wiyono S, Dadang. 2019. Aktivitas metabolit sekunder cendawan endofit terhadap Colletotrichum acutatum pada cabai merah. Jurnal Fitopatologi Indonesia. 15(1):36–44. DOI: https://doi.org/10.14692/jfi.15.1.36.
Shahzad M, Sherry L, Rajendran R, Edwards CA, Combet E, Ramage G. 2014. Utilising polyphenols for the clinical management of Candida albicans biofilms. International Journal of Antimicrobial Agents. 44(3):
–273. DOI: https://doi.org/10.1016/j.ijantimicag.2014.05.017.
Shu C, Chen Q, Pi L, Zhang D, Pahwar QA, Zhou E. 2017. Identification and antifungal activity analysis of two biocontrol antagonists to Colletotrichum musae. Journal of Phytopathology. 165(7–8):554–561. DOI: https://doi.org/10.1111/jph.12592.
Sudibyo D. 1994. Petunjuk Praktis Cara Menghitung Jumlah, Kerapatan dan Viabilitas Spora Jamur. Laboratorium Utama Pengendalian Hayati, Dinas Perkebunan Propinsi Jawa Timur.
Suganda T, Simarmata INC, Supriyadi Y, Yulia E. 2019. Uji in-vitro kemampuan ekstrak metanol bunga dan daun tanaman kembang telang (Clitoria ternatea L.) dalam menghambat pertumbuhan jamur Fusarium oxysporum f.sp. cepae. Jurnal Agrikultura. 30(3):109–116. DOI: https://doi.org/10.24198/agrikultura.v30i3.24031.
Sulaiman. 2013. Efektifitas pemberian ekstrak etanol 70% daun kecombrang (Etlingera elatior) terhadap larva instar III Aedes aegepty sebagai larvasida potensial [skripsi]. Lampung (ID): Universitas Lampung.
Sundari. 2012. Suatu modul pengembangan media pembelajaran slide culture untuk pengamatan struktur mikroskopis kapang pada matakuliah mikologi. Jurnal Bioedukasi. 1(1):39–47.
Sutton BC. 1992. The Genus Glomerella and its Anamorph Colletotrichum. Di dalam: Bailey JA, Jeger MJ, editor. Colletotrichum: Biology, Pathology and Control. Wallingford (UK): CAB International. hlm 1-25.
Syamsuri S, Alang H. 2021. Inventarisasi Zingiberaceae yang bernilai ekonomi (etnomedisin, etnokosmetik, dan etnofood) di Kabupaten Kolaka Utara, Sulawesi Tenggara, Indonesia. Agro Bali: Agricultural Journal. 4(2):219–229. DOI: https://doi.org/10.37637/ab.v4i2.715.
Triasih U, Agustina D, Erti MD, Wuryantini S. 2019. Uji berbagai bahan pembawa terhadap viabilitas dan kerapatan konidium pada beberapa biopestisida cair jamur entomopatogen. Jurnal Agronida. 5(1):12–20.
Widiyantoro A, Harlia. 2020. Aktivitas antioksidan ekstrak daun kenikir (Cosmos caudatus Kunth) dengan berbagai metode ekstraksi. Indonesian Journal of Pure and Applied Chemistry. 3(1):9–14. DOI: https://doi.org/10.26418/indonesian.v3i1.46519.
Wijekoon MMJO, Bhat R, Karim AA. 2011. Evaluation of nutritional quality of torch ginger (Etlingera elatior Jack.) inflorescence. Journal of Internatinal Food Research. 18(4):1415–1420.
Wijekoon MMJO, Bhat R, Karim AA, Fazilah A. 2013. Chemical composition and antimicrobial activity of essential oil and solvent extracts of torch ginger inflorescence (Etlingera elatior Jack.). International Journal of Food Properties. 16(6):1200–1210. DOI: https://doi.org/10.1080/10942912.2011.579674.
Yendi TP, Efri, Prasetyo J. 2015. Pengaruh ekstrak beberapa tanaman famili Zingiberaceae terhadap penyakit antraknosa pada buah pisang. Jurnal Agrotek Tropika. 3(2):231–235. DOI: https://doi.org/10.23960/jat.v3i2.2003.
Copyright (c) 2024 Jurnal Fitopatologi Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish in Jurnal Fitopatologi Indonesia agree to the following terms:
1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository), with an acknowledgement of its initial publication in this journal.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.