Induksi Tunas Ubi Kayu (Mannihot esculentaCrantz.) var. Adira 2 Secara In vitro

Abstract

The research aim was to evaluate the effect of culture medium compositon and BAP concentration on growth and shoot multiplication of in vitro-grown cassava (Mannihot esculentaCrantz.) var. ‘Adira 2’. The experimental design was completely randomize design with two factors. The first factor was composition of basal medium including MS and ½ MS (half of macro and micro nutrients), and the second factor was concentration of BAP (0, 0.5, 1, 1.5, and 3 ppm). The result showed that basal medium MS was the best medium to induce shoot multiplication of cassava var. ‘Adira 2’ with 1.27 shoots explant-1. BAP concentrations had no significant effect on some variables including number of shoot, leaf, node, and height
of shoot. However, the BAP concentration affected the number of root explant-1. The control treatment medium (without BAP) showed the best growth of root with 1.95 roots explant-1.
Keywords: cassava, BAP, MS medium, tissue culture