Evaluasi Metabolomik Mutan Putatif Bawang Putih (MV3) Hasil Iradiasi Gamma LD50

Abstract

The increase in the genetic diversity of garlic can be through artificial mutation breeding using gamma rays. The aim of the experiment was to study the effect of gamma irradiation LD50 dose on growth, production, and content of metabolites of the MV3 generation of garlic genotypes. The research was carried out from May to September 2020 at the Pasir Sarongge Experimental Garden, IPB. The experiment consisted of six mutant populations of garlic genotypes without replication, namely Ciwidey 0 Gy, Ciwidey 6 Gy, Tawangmangu Baru 0 Gy, Tawangmangu Baru 8 Gy, Lumbu Kuning 0 Gy, Lumbu Kuning 10 Gy. Each population had a different number of individuals depending on the availability of planting material from the previous generation. A total of 364 plants were planted in the field for observation of plant growth and production. The results showed that exposure to LD50 doses in all genotypes of garlic decreased tuber diameter and number of cloves compared to controls. The new compounds detected at CW 6 Gy were 2-Hexanol; 2-Hexanol,3,4-dimethyl-; Cyclopentene,1,2,3,3,4-pentamethyl; Trisulfide,methyl2-propyl. New compounds detected in LK 10 Gy are Butyl isobutyl phthalate; 2-Pentanol, 2-methyl-; Ether,2-chloro-1-methylethyl isopropyl; Hentricontane; Isoamyllactate. New compounds detected in 8 Gy TMB; Cyclopentene, 1,2, 3,3,4-pentamethyl-; Decane; 3-Vinyl-1,2-dithiacyclohex-4-ene; Diallyl disulphide.

Keywords: allicin, antimicrobial, GC-MS, metabolomics, mutant