STUDI PENDAHULUAN ENZIM KITINASE EXTRASELULER YANG DIHASILKAN  OLEH ISOLAT BAKTERI ASAL MANADO 1)  [Preliminary Study of Extracellular Chitinase Produced by Bacteria Isolated from Manado]

E. Y. Purwani; A. Toharisman; E. Chasanah; J. F. Laksmi; V. Welan; M. T. Suhartono; T. Purwadaria; J. K. Hwang; Y. R. Pyun

E. Y. Purwani⁽¹⁾ , A. Toharisman⁽²⁾ , E. Chasanah⁽³⁾ , J. F. Laksmi⁽⁴⁾ , V. Welan⁽⁵⁾ , M. T. Suhartono⁽⁶⁾ , T. Purwadaria⁽⁷⁾ , J. K. Hwang⁽⁸⁾ , Y. R. Pyun⁽⁹⁾

(1) PAU Bioteknologi IPB Jl. Puspa 1 Institut Pertanian Bogor, Bogor 16610,

(2) PAU Bioteknologi IPB Jl. Puspa 1 Institut Pertanian Bogor, Bogor 16610,

(3) PAU Bioteknologi IPB Jl. Puspa 1 Institut Pertanian Bogor, Bogor 16610,

(4) PAU Bioteknologi IPB Jl. Puspa 1 Institut Pertanian Bogor, Bogor 16610,

(5) PAU Bioteknologi IPB Jl. Puspa 1 Institut Pertanian Bogor, Bogor 16610,

(6) PAU Bioteknologi IPB Jl. Puspa 1 Institut Pertanian Bogor, Bogor 16610,

(7) Balai Penelitian Ternak Ciawi, Bogor,

(8) Bioproduct Research Center, Yonsei University Seoul, Korea,

(9) Bioproduct Research Center, Yonsei University Seoul, Korea

Issue
Vol. 13 No. 2 (2002): Jurnal Teknologi dan Industri Pangan

Submitted
February 29, 2012

Published
March 2, 2012

Keywords:

Chitin, chitinase

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Abstract

Chitinolytic bacteria were isolated from several exotic area in Manado Province. The most potential isolate, namely 13.26, was isolated from Tompaso. The isolate was cultured in the thermus medium containing colloidal chitin as a carbon source for 5 days at 55°C to produce chitinase. It was observed that chitinase was most active at 65°C and the optimum pH was 8 in boric acid-borax buffer. Ammonium sulfate (50% saturation) precipitation of the protein increased the specific activity of the enzyme from 0.20 unit/mg protein (in culture supernatant) to 0.28 unit/mg protein. The molecular weight as estimated by zymogram analysis was180 kDa.