PENGARUH BERBAGAI KONDISI PREPARASI DAN PENYIMPANAN SUSU FORMULA PADA PERTUMBUHAN SPORA Bacillus cereus DAN Clostridium perfringens [The growth of Bacillus cereus and Clostridium perfringens spores under a variety of preparation and storage condition]

Abstract

The aim of this study was to evaluate the ability of Bacillus cereus and Clostridium perfringens spores to survived in different preparation temperature (25, 35, and 70°C for B. cereus and 25, 45, 70ºC for C. perfringens) and modified storage condition (with 50 and 75% humidity; opened, closed and opened twice a day during period of observation) of powdered milk  formula.  The spores of B. cereus ATCC 13061 and         C. perfringens CP-1 artificially  contaminated to the milk formula. Results showed that B. cereus and C. perfringens spores were germinated and growth in reconstituted milk formula.  Bacteria population increased ≥1 log within 3 hours at room temperature (28-29ºC).  The spores of both bacteria survived from dry condition of powdered milk formula, like when aw of the formulas increased cause of storage condition.