Pengaruh konsentrasi asam klorida terhadap ekstraksi glukosamina dari kitosan Penaeus monodon sebagai sistem sediaan liposom The effect of hydrochloric acid concentration on glucosamin extraction from chitosan Penaeus monodon as liposomal-based delivery system
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chitosan, glucosamine, hydrolysis, nanoparticle, sonication
Abstract
Glucosamine is an important compound for maintaining joint health. Currently, almost all glucosamine needs in Indonesia still depend on imports. Therefore, developing glucosamine production with variations in HCl concentration is a strategic step to meet national needs. The study's goal is to find the best HCl concentration for breaking down chitosan using ultrasonic extraction to make glucosamine hydrochloride (GlcN-HCl) and putting it in a liposome system. Extraction was carried out with variations in HCl concentration of 2%, 4%, and 6%, with the hope of obtaining the best yield. The hydrolysis process was carried out with ultrasonic treatment for 40 minutes. The results indicated that the ultrasonic extraction method with variations in HCl concentration produced GlcN-HCl with good physicochemical characteristics. The yield obtained ranged from 78.95 to 78.95–86.20%. The resulting GlcN-HCl has a pH of 3.9–3.64 and a specific crystal structure at an angle of 2θ (9.87° and 28.95°) based on XRD analysis. The FTIR spectrum showed a characteristic absorption at wave number 1613 cm⁻¹, indicating the presence of amine groups. The particle size of GlcN-HCl ranged from 26 to 26–239 nm. Evaluation of the stability of liposomes containing GlcN-HCl during 4 days of storage showed that storage time affected the physical stability of liposomes, which was indicated by an increase in turbidity.
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