Extraction and characterization of acid-soluble collagen and pepsin-soluble collagen from the dry scales of the striped snakehead (Channa striatus)

Bagus Fajar Pamungkas (1) , Supriyadi Supriyadi (2) , Agnes Murdiati (2) , Retno Indrati (2)
(1) Program Studi Teknologi Hasil Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Mulawarman, Samarinda Departemen Teknologi Pangan dan Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Gadjah Mada, Indonesia,
(2) Departemen Teknologi Pangan dan Hasil Pertanian, Fakultas Teknologi Pertanian, Indonesia
https://doi.org/10.17844/jphpi.v21i3.24734

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JPHPI 21(3)
Issue
Vol. 21 No. 3 (2018): Jurnal Pengolahan Hasil Perikanan Indonesia
Submitted
December 31, 2018
Published
December 28, 2018
Keywords:
    Extraction methods, haruan scale, protein pattern, type I collagen
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Abstract

Characteristics of collagen are influenced by the source of raw materials and extraction methods used. The aim of this research was to characterize the acid- and pepsin-soluble collagens from the dry scales of the striped snakehead (Channa striatus). Collagen was extracted using to methods including 0.5 M acetic acid and 0.1% pepsin. The yield of acid soluble collagen (KLA-SH) and pepsin soluble collagen (KLP-SH) were 0.98% and 1.94%, respectively. KLA-SH and KLP-SH contained glycine as the major amino acid and had high imino acid group content i.e 226 and 230 residues/1.000 residues, respectively. FTIR spectra of KLA-SH and KLP-SH showed that of the structure of collagen could be maintained in the form of triple helix structure. KLA-SH and KLP-SH consisted of α1- and α2-chain, β-chain, and γ-chain and is suggested as type I collagen.

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Authors

Bagus Fajar Pamungkas
Program Studi Teknologi Hasil Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Mulawarman, Samarinda Departemen Teknologi Pangan dan Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Gadjah Mada, Indonesia
indrati@ugm.ac.id (Primary Contact)
Supriyadi Supriyadi
Departemen Teknologi Pangan dan Hasil Pertanian, Fakultas Teknologi Pertanian, Indonesia
Agnes Murdiati
Departemen Teknologi Pangan dan Hasil Pertanian, Fakultas Teknologi Pertanian, Indonesia
Retno Indrati
Departemen Teknologi Pangan dan Hasil Pertanian, Fakultas Teknologi Pertanian, Indonesia
Pamungkas, B. F., Supriyadi, S., Murdiati, A., & Indrati, R. (2018). Extraction and characterization of acid-soluble collagen and pepsin-soluble collagen from the dry scales of the striped snakehead (Channa striatus). Jurnal Pengolahan Hasil Perikanan Indonesia, 21(3), 513-521. https://doi.org/10.17844/jphpi.v21i3.24734
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Pamungkas, B. F., Supriyadi, S., Murdiati, A., & Indrati, R. (2018). Extraction and characterization of acid-soluble collagen and pepsin-soluble collagen from the dry scales of the striped snakehead (Channa striatus). Jurnal Pengolahan Hasil Perikanan Indonesia, 21(3), 513-521. https://doi.org/10.17844/jphpi.v21i3.24734
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