The Association of SNP g.49170107 G>T in the CYP2A6 Gene with Mineral Content in Sheep

Abstract

Mineral content plays a pivotal role in determining the quality of meat, which consumers prioritize
when seeking healthy and high-quality lamb. Enhancing the genetic makeup of mineral content is
therefore crucial to pique consumers’ interest in lamb meat. Among the potential genes that could be
used as markers for the nutritional quality, including the mineral content, of Indonesian lamb meat, the
cytochrome P450, family 2, subfamily A, polypeptide 6 (CYP2A6) gene stand out. The main objective
of this study was to identify the CYP2A6 gene polymorphism at SNP g.49170107 G>T and explore its
association with mineral content in Indonesian sheep. For this purpose, 95 sheep samples were analyzed,
consisting of 81 Javanese thin-tail sheep (JTTS) and 14 Jonggol sheep (JS). The researchers investigated
the polymorphism through the PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length
Polymorphism) method. Subsequently, they analyzed the association between the CYP2A6 gene and
mineral content in lamb meat using the GLM (General Linear Model) method. The study revealed
that the CYP2A6 gene polymorphism was present in JTTS, while it remained monomorphic in JS.
The identified genotypes in the polymorphism were GG and GT. Notably, the CYP2A6 gene exhibited
a significant association (P<0.05) with Fe content. Specifically, the GT genotype displayed higher Fe
content compared to the GG genotype. These findings suggest that the CYP2A6 gene holds promise as a
potential candidate for identifying mineral content in lamb meat.