Evaluation of Selected Intestinal Damage Biomarkers for the Determination of Intestinal Epithelial Damage in Neonatal Lambs with Diarrhea
Abstract
The aim of this study was to evaluate selected intestinal damage biomarkers for the determination of intestinal mucosal epithelial barrier damage in neonatal lambs with diarrhea. The study material consisted of 68 lambs with diarrhea (experimental group, 39 Escherichia coli infected lambs and 29 Cryptosporidium infected lambs) and 20 healthy lambs (control group) aged between 2 and 20 days. The diseases were diagnosed on the basis of a positive fecal antigen test for E. coli K 99, Rotavirus, Coronavirus, Cryptosporidium, and Giardia species in fecal samples obtained from lambs with diarrhea and clinical signs. In addition, Cryptosporidium oocysts were confirmed by light microscopic examination. Anticoagulated blood samples for hemogram measurements and non-anticoagulated blood samples for biomarker measurements were collected from all the lambs. Serum intestinal fatty acid binding protein (I-FABP), trefoil factor-3 (TFF-3), intestinal alkaline phosphatase (IAP), and claudin-3 (CLDN-3) biomarkers were measured using sheep specific enzyme-linked immunosorbent assay (ELISA) test kits. Standard diarrhea treatment was applied to lambs with diarrhea. While 57 lambs recovered, 11 died. There was a significant increase in serum I-FABP, TFF-3, and IAP concentrations (p<0.001) and a decrease in serum CLDN-3 concentrations (p<0.001) in lambs with diarrhea compared to healthy lambs. Total white blood cell (WBC), granulocyte (GRA), and monocyte (MON) counts increased in lambs with diarrhea compared to healthy lambs (p<0.05). In conclusion, this study demonstrated that I-FABP, TFF-3, IAP, and CLDN-3 were useful and reliable biomarkers to determine the presence and extent of intestinal mucosal epithelial damage in lambs with diarrhea.
References
Ay, C. D., Tuna, G. E., Asici, G. S. E., Ulutas, B., & Voyvoda, H. (2022). Serum intestinal fatty acid-binding protein and calprotectin concentrations to assess clinical severity and prognosis of canine parvovirus enteritis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28(1), 105-114.
Brown, C. C., Baker, D. C., & Barker, I. K. (2019). Alimentary system. In Jubb, K.V.F., Kennedy, P. C., & Palmer, N. (ed.) Pathology of domestic animals. 6th ed. (pp. 1-257). Elsevier.
Davis, E., Machugh, N., Kenny, D., & Ward, P. (2016). The effect of feeding colostrum at different volumes and subsequent number of transition milk feeds on the serum immunoglobulin G concentration and health status of dairy calves. Journal of Dairy Science, 97(11), 6991-7000. https://doi.org/10.3168/jds.2013-7494
Durgut, M. K., & Ok, M. (2023). Evaluation of some intestinal biomarkers in the determination of intestinal damage in calves with coccidiosis. Tropical Animal Science Journal, 46(2), 221-230. https://doi.org/10.5398/tasj.2023.46.2.221
Ekici, Y. E., & Ok, M. (2024). Investigation of the relationship between atopic dermatitis dogs and intestinal epithelial damage. Veterinary Medicine and Science, 10(3), e1453. https://doi.org/10.1002/vms3.1453
Foreyt, W. J. (2013). Veterinary parasitology reference manual (pp. 3-10). John Wiley & Sons.
Fortuoso, B. F., Gomes, C. K., Santos, J. P., Salle, C. T. P., Moresco, R. N., Kommers, G. D., & Barcellos, D. E. S. (2019). Enteritis in lambs associated with Clostridium perfringens type A and E. Pesquisa Veterinaria Brasileira, 39(2), 97-102.
Gulersoy, E., Ok, M., Uney, K., Durgut, M. K., Parlak, M. T., & Ekici, Y. E. (2023). Intestinal damage and vasculitis biomarkers in cats with feline enteric coronavirus and effusive feline infectious peritonitis. Veterinary Medicine and Science, 9(6), 2420-2429. https://doi.org/10.1002/vms3.1299
Gulersoy, E., Ok, M., Yildiz, R., Koral, E., Ider, M., Sevinc, M., & Zhunushova, A. (2020). Assessment of intestinal and cardiac-related biomarkers in dogs with parvoviral enteritis. Polish Journal of Veterinary Science, 23(2), 211-219. https://doi.org/10.24425/pjvs.2020.133635
Ider, M., Nasari, A., Ok, M., Ertürk, A., Durgut, M. K., & İyigün, S. S. (2023). Surveilling brain damage using brain biomarkers in hypoglycemic neonatal calves with diarrhea. Frontiers Veterinary Science, 10, 1240846. https://doi.org/10.3389/fvets.2023.1240846
Johnson, J. L., Kuskie, K. R., Smith, B. A., Hostetter, J. M., & Evans, T. J. (2017). Cryptosporidiosis in neonatal lambs in the United States. Journal of Veterinar Diagnostic Investigation, 29(1), 87-90.
Nieto, J. E., Aldridge, B. M., Beldomenico, P. M., Aleman, M., & Snyder, J. R. (2005). Characterization of equine intestinal fatty acid binding protein and its use in managing horses with colic. American Journal of Veterinary Research, 66(2), 223-232. https://doi.org/10.2460/ajvr.2005.66.223
Ok, M., Yıldız, R., Hatipoğlu, F., Başpınar, N., İder, M., Üney, K., Ertürk, A., Durgut, M. K., & Terzi, F. (2020). Use of intestine-related biomarkers for detecting intestinal epithelial damage in neonatal calves with diarrhea. American Journal of Veterinary Research, 81(2), 139-146. https://doi.org/10.2460/ajvr.81.2.139
Smith, M. C., Sherman, D. M., & Henderson, D. C. (2019). Diarrhea in neonatal ruminants. In Smith, M. C., Sherman, D. M (eds.) Goat medicine, (2nd ed.), (pp. 215-238). Hoboken: Wiley-Blackwell.
Yıldız, İ. B., & Ok, M. (2022). Evaluation of selected intestinal damage biomarkers to determine intestinal damage in dogs with isosporosis. Magyar Allotorvosok Lapja, 144, 101-113.
Yildiz, R., Ok, M., Ider, M., Aydogdu, U., Naseri, A., Parlak, K., & Gulersoy, E. (2018). Evaluation of intestinal damage biomarkers in calves with atresia coli. Journal of Veterinary Research, 62(3), 379-384. https://doi.org/10.2478/jvetres-2018-0054
Yildiz, R., Ok, M., Ider, M., Akar, A., Naseri, A., & Koral, E. (2019). The changes in biomarkers for necrotising enterocolitis in premature calves with respiratory distress syndrome. Veterinarni Medicina, 64(10), 440-447. https://doi.org/10.17221/37/2019-VETMED
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