Variations in Semen Quality and Potential for Frozen Semen Production in Aceh Cattle
Abstract
The quality of fresh and frozen semen as well as the potential to produce frozen semen, vary considerably between individual animals and cattle breeds. This study aimed to analyze the quality of fresh and frozen semen in Aceh cattle and calculate the potential production of frozen semen in Aceh bulls over 1 year. This study used primary data on the quality of fresh and frozen semen from five Aceh cattle and obtained secondary data from artificial insemination centers in Lembang and Singosari in 2022. Semen samples were collected weekly, and the quality of fresh and frozen semen samples were evaluated. The data were analyzed using a one-way analysis of variance at 95% significance level, followed by Tukey’s test. The results revealed differences in semen volume and pH. Based on microscopic characteristics, the semen samples from different bulls exhibited no discernible differences in mass movement, sperm motility, viability, morphology, and plasma membrane integrity, except for sperm concentration and acrosomal integrity. Bull 211605 exhibited the highest sperm concentration. Furthermore, the frozen semen samples from Aceh cattle showed no significant differences in viability, plasma membrane integrity, morphology, and acrosomal integrity. The total sperm motility of bull 211710 was higher than that of the other bulls. The highest progressive motility was observed in bulls 211710 and 211605, and the highest intact DNA was detected in bulls 211710 and 211605. The results of this study demonstrated that the quality of fresh and frozen semen in Aceh cattle is distinct, and the potential frozen semen production of Aceh cattle is estimated to range from 3,382 ± 1810 to 11,399 ± 2658 straws/year.
References
Arifiantini, I. (2012). Teknik koleksi dan evaluasi semen pada hewan. IPB Press.
Agarwal, A., Gupta, S., & Sharma, R. (2016). Sperm morphology stain (Diff-Quik®). In Agarwal, A., Gupta, S., Sharma, R. (Eds.), Andrological Evaluation of Male Infertility (pp 79–82). Springer. https://doi.org/10.1007/978-3-319-26797-5_9
Baharun, A., Arifiantini, R. I., Karja, N. W. K., & Said, S. (2020). Seminal plasma protein profile is based on molecular weight and its correlation with the semen quality of Simmental bull. Journal Indonesian Tropical Animal Agriculture, 46(1), 20-28. https://doi.org/10.14710/jitaa.46.1.20-28
Badan Standardisasi Nasional. (2021). SNI (Standar Nasional Indonesia) Semen beku - bagian 1, sapi, S. N. I. 4869.1-2021. Badan Standardisasi Nasional.
Coulter, G. H., & Foote, R. H. (1977). Relationship of body weight to testicular size and consistency in growing Holstein bulls. Journal of Animal Science, 44(6), 1076-1079. https://doi.org/10.2527/jas1977.4461076x
Handayani, S., & Safrida, S. (2023). Characteristics of cattle farm breeders in Aceh Province. IOP Conference Series Earth and Environmental Science, 1183(1), 012037. https://doi.org/10.1088/1755-1315/1183/1/012037
Indriastuti, R., Ulum, M. F., Arifiantini, R. I., & Purwantara, B. (2020). Individual variation in fresh and frozen semen of Bali bulls (Bos sondaicus). Veterinary World, 13(5), 840-846. https://doi.org/10.14202/vetworld.2020.840-846
Iskandar, H., Sonjaya, H., Arifiantini, R. I., & Hasbi, H. (2022). Correlation between semen quality. Libido testosterone concentration Bali bulls. Jurnal Ilmu Ternak dan Veteriner, 27(2), 57-64. https://doi.org/10.14334/jitv.v27i2.2981
Isnaini, N., Wahjuningsih, S., & Adhitama, E. (2019). Seasonal effects on sperm quality of Ongole crossbred and simmental bulls used for artificial insemination. Livestock Research for Rural Development, 31(2), 16.
Kavak, A., Lundeheim, N., Aidnik, M., & Einarsson, S. (2004). Sperm morphology in Estonian and Tori breed stallions. Acta Veterinaria Scandinavica, 45(1-2), 11-18. https://doi.org/10.1186/1751-0147-45-11
Prochowska, S., Niżański, W., & Fontbonne, A. (2022). Hypo-osmotic swelling test (HOST) for feline spermatozoa: the simplified procedure and the aspect of sperm morphology. Animals, 12(7), 903. https://doi.org/10.3390/ani12070903
Rajabi-Toustani, R., Akter, Q. S., Almadaly, E. A., Hoshino, Y., Adachi, H., Mukoujima, K., & Murase, T. (2019). Methodological improvement of fluorescein isothiocyanate peanut agglutinin (FITC-PNA) acrosomal integrity staining for frozen-thawed Japanese Black bull spermatozoa. Journal of Veterinary Medical Science, 81(5), 694-702. https://doi.org/10.1292/jvms.18-0560
Rashid, M. M., Hoque, M. A., Huque, K. S., & Bhuiyan, A. K. F. H. (2015). Effect of semen collection frequency and scrotal circumference on semen quality parameters in Brahman x Local crossbred bulls. Advances in Animal and Veterinary Sciences, 3(12), 677-684. https://doi.org/10.14737/journal.aavs/2015/3.12.677.684
Roadmap Swasembada Pejantan Unggul 2018-2022. (2018). Direktorat Perbibitan dan Produksi Ternak Direktorat Jenderal Peternakan dan Kesehatan Hewan Kementerian Pertanian.
Rodrigues, N. N., Vrisman, D. P., Rossi, G. F., Freitas, A. P., Zorzetto, M. F., Souza, L. L., Taira, A. R., Vicente, W. R. R., Monteiro, F. M., & Oliveira, M. E. F. (2019). 152 correlation between testicular and accessory sex glands biometric characteristics in Nellore and Caracu bulls. Reproduction, Fertility and Development, 31(1), 201-201. https://doi.org/10.1071/RDv31n1Ab152
Said, S., Afiati, F., & Maulana, T. (2015). Study on changes of sperm head morphometry and DNA integrity of freeze-dried bovine sperm. Journal of the Indonesian Tropical Animal Agriculture, 40(3), 145-152. https://doi.org/10.14710/jitaa.40.3.145-152
Santoso, S., Herdis, H., Arifiantini, R. I., Gunawan, A., & Sumantri, C. (2021). Characteristics and potential production of frozen sperm of Pasundan bull. Tropical Animal Science Journal, 44(1), 24-31. https://doi.org/10.5398/tasj.2021.44.1.24
Simões, R., Feitosa, W. B., Mendes, C. M., Marques, M. G., Nicacio, A. C., de Barros, F. R. O., Visintin, J. A., & Assumpção, M. E. O. A. (2009). Use of chromomycin A3 staining in bovine sperm cells for detection of protamine deficiency. Biotechnic & Histochemistry, 84(3), 79-83. https://doi.org/10.1080/10520290902843595
Sukmawati, E., Arifiantini, R. I., & Purwantara, B. (2014). Freezing capacity of sperm on various type of superior bulls. Jurnal Ilmu Ternak dan Veteriner, 19(3), 168-175. https://doi.org/10.14334/jitv.v19i3.1079
Wahyudi, I., Qalfin, M., Susanti, R., & Widiatningrum, T. (2022). Relationship between scrotal circumference and quality of semen production in bulls: a meta-analysis review. Jurnal Sain Peternakan Indonesia, 17(3), 159-169. https://doi.org/10.31186/jspi.id.17.3.159-169
Zuidema, D., Kerns, K., & Sutovsky, P. (2021). An exploration of current and perspective semen analysis and sperm selection for livestock artificial insemination. Animals, 11(12), 3563. https://doi.org/10.3390/ani11123563
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