The Quality of Cryopreserved Semen of Local Chickens Treated with Ringer’s Lactate-Egg Yolk Extender Supplemented with Glycine and Glucose
Abstract
The aim of this study is to investigate the potential of glucose, glycine, and a combination of glucose and glycine supplemented into Ringer’s lactate extender on the quality of pre- and post-cryopreservation of semen of local chickens. Semen samples were collected from KUB roosters. The research procedures involved formulation of the extender, semen collection, assessment of fresh semen, dilution, packaging, cryopreservation, storage, and subsequent assessment of cryopreserved semen. Parameters assessed in fresh, pre-cryopreservation, and post-thawed semen using computer-assisted semen analysis (CASA), included motility (total motility, progressive, static, and slow motilities), kinematics, spermatozoa morphology, malondialdehyde (MDA) concentration, and scanning electron microscopy (SEM). There were four treatments: T1= control, T2= 50 mM glucose, T3= 60 mM glycine, and T4= a combination of 50 mM glucose and 60 mM glycine. Each treatment was repeated 10 times. The results showed that the addition of 60 mM glycine and the combination of 50 mM glucose and 60 mM glycine effectively preserved total motility, maintained progressive motility, and reduced damage to the bent tail during the equilibration process (prior to cryopreservation). In addition, total motility, progressive motility, DAP, DSL, DCL, VAP, VSL, VCL, STR, and LIN were significantly increased after thawing. This approach also minimized the formation of MDA. Furthermore, observations using SEM showed comparatively more intact acrosomes compared to the other treatments. In conclusion, the addition of 60 mM glycine and a combination of 60 mM glycine and 50 mM glucose to Ringer’s lactate-egg yolk extender proved to be effective in preserving the quality of domestic chicken spermatozoa during semen cryopreservation.
References
Blank, M. H., L. P. Ruivo, G. A. Novaes, E. C. Lemos, J. D. Losano, A. F. Siqueira, & R. J. Pereira. 2021. Assessing different liquid-storage temperatures for rooster spermatozoa. Anim. Reprod. Sci. 233:106845. https://doi.org/10.1016/j.anireprosci.2021.106845
Cheng, C. H., F. F. Yang, S. A. Liao, Y. T. Miao, C. X. Ye, A. L. Wang, & X. Y. Chen. 2015. High temperature induces apoptosis and oxidative stress in pufferfish (Takifugu obscurus) blood cells. J. Therm. Biol. 53:172-179. https://doi.org/10.1016/j.jtherbio.2015.08.002
Farooq, U., I. A. Malecki, M. Mahmood, & G. B. Martin. 2017. Appraisal and standardization of curvilinear velocity (VCL) cut-off values for CASA analysis of Japanese quail (Coturnix japonica) sperm. Reprod. Domest. Anim. 52:389–396. https://doi.org/10.1111/rda.12920
Fattah, A., M. Sharafi, R. Masoudi, A. Shahverdi, & V. Esmaeili. 2017. L-carnitine is a survival factor for chilled storage of rooster semen for a long time. Cryobiology 74:13-18. https://doi.org/10.1016/j.cryobiol.2016.12.011
Fujita, Y., M. Nishimura, N. W. Komori, T. Wada, C. Shirakawa, T. Takenawa, T, & M. Doi. 2020. A pair of cell preservation solutions for therapy with human adipose tissue-derived mesenchymal stromal cells. Regen. Ther. 14:95-102. https://doi.org/10.1016/j.reth.2019.10.004
Henuk, Y. L. & D. Bakti. 2018. Benefits of promoting native chickens for sustainable rural poultry development in Indonesia. Talenta Conference Series Agricultural Natural Resources 1:69-76. https://doi.org/10.32734/anr.v1i1.98
Heydari, M., B. Qasemi-Panahi, M. Gh, H. Daghigh-Kia, & R. Masoudi. 2021. Conservation of buck’s spermatozoa during cooling storage period through cooling medium supplementation with L-Carnitine. Arch. Razi Inst. 76:1797.
Iswati, N. Isnaini, & T. Susilawati. 2018. Effect of addition of glutathione in diluent ringer’s on spermatozoa quality of domestic chicken during cold storage. Asian J. Microbiol. Biotechnol. Environ. Sci. 20:12-20.
Junaedi, R. I. Arifiantini, C. Sumantri, & A. Gunawan. 2016. Use of glycerol as cryoprotectants in freezing Sentul chicken semen. Chalaza Journal Animal Husbandry 1:6-13. https://doi.org/10.31327/chalaza.v1i2.165
Junaedi, R. I. Arifiantini, & C. Sumantri. 2017. The quality of Kampung broiler (KB) chicken frozen semen with DMA concentrations on yolk lactate ringer diluent. Chalaza Journal Animal Husbandry 2:19-24. https://doi.org/10.31327/chalaza.v2i2.292
Khaeruddin, K., S. Wahjuningsih, G. Ciptadi, M. Yusuf, H. Hermawansyah, & S. Sahiruddin. 2022. Cryopreservation of Gaga’chicken semen from South Sulawesi, Indonesia with the addition of L-carnitine, hyaluronic acid, sucrose and their combination in diluent. Biodiversitas 23:3297-3302. https://doi.org/10.13057/biodiv/d230659
Khaeruddin, Junaedi, & Hastuti. 2020. Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants. Biodiversitas 21:5718-5722. https://doi.org/10.13057/biodiv/d211231
Kheawkanha, T., V. Chankitisakul, P. Thananurak, M. Pimprasert, W. Boonkum, & T. Vongpralub. 2023. Solid storage supplemented with serine of rooster semen enhances higher sperm quality and fertility potential during storage at 5 °C for up to 120 h. Poult. Sci. 102:102648. https://doi.org/10.1016/j.psj.2023.102648
Kheawkanha, T., W. Boonkum, T. Vongpralub, & V. Chankitisakul. 2021. Characterization of oviduct lining, with emphasis on the sperm storage tubule region (uterovaginal junction), correlated with fertility in mature and old Thai native hens. Animals 11:3446. https://doi.org/10.3390/ani11123446
Kumar, K. P., B. Swathi, & M. Shanmugam. 2019. Effect of L-glycine and L-carnitine on post-thaw semen parameters and fertility in chicken. Slovak Journal Animal Science 52:1–8.
Kutluyer, F. & M. Kocabas. 2016. Use of amino acids in fish sperm cryopreservation: A review. Austin Biology 1:1014.
Kuzlu, M. & A. Taskin. 2017. The effect of different extenders on the sperm motility and viability of frozen Turkey semen. Indian J. Anim. Res. 51:235-241. https://doi.org/10.18805/ijar.v0iOF.6825
Lotfi, S., M. Mehri, M. Sharafi, & R. Masoudi. 2017. Hyaluronic acid improves frozen-thawed sperm quality and fertility potential in rooster. Anim. Reprod. Sci. 184:204-210. https://doi.org/10.1016/j.anireprosci.2017.07.018
Madeddu, M., F. Mosca, A. A. Sayed, L. Zaniboni, M. G. Mangiagalli, E. Colombo, & S. Cerolini. 2016. Effect of cooling rate on the survival of cryopreserved rooster sperm: Comparison of different distances in the vapor above the surface of the liquid nitrogen. Anim. Reprod. Sci. 171:58–64. https://doi.org/10.1016/j.anireprosci.2016.05.014
Manjunath, P. 2018. New insights into the understanding of the mechanism of sperm protection by extender components. Anim. Reprod. 9:809-815.
Masoudi, R., M. Sharafi, A. Z. Shahneh, & M. Khodaei-Motlagh. 2019. Effects of reduced glutathione on the quality of rooster sperm during cryopreservation. Theriogenology 128:149-155. https://doi.org/10.1016/j.theriogenology.2019.01.016
Moreno, J. S. & D. A. G. Lucero. 2019. Criopreservación de espermatozoides en especies domésticas y silvestres: estado actual de los avances tecnológicos. Revista Ecuatoriana Ciencia Animal. 3:18-38.
Mussa, N. J., R. Ratchamak, T. Ratsiri, T. Vongpralub, W. Boonkum, Y. Semaming, & V. Chankitisakul. 2021. Lipid profile of sperm cells in Thai native and commercial roosters and its impact on cryopreserved semen quality. Trop. Anim. Health Prod. 53:321. https://doi.org/10.1007/s11250-021-02664-9
Nabi, M. M., H. Kohram, M. Zhandi, H. Mehrabani-Yeganeh, H. Sharideh, A. Zare-Shahaneh, & V. Esmaili. 2016. Comparative evaluation of Nabi and Beltsville extenders for cryopreservation of rooster semen. Cryobiology 72:47-52. https://doi.org/10.1016/j.cryobiol.2015.11.005
Nguyen, T. M. D., F. Seigneurin, P. Froment, Y. Combarnous, & F. Blesbois. 2015. The 5’-AMP-activated protein kinase (AMPK) is involved in the augmentation of antioxidant defenses in cryopreserved chicken sperm. PloS ONE 10:0134420. https://doi.org/10.1371/journal.pone.0134420
Partyka, A., O. Rodak, J. Bajzert, J. Kochan, & W. Niżański. 2017. The effect of L-Carnitine, hypotaurine, and taurine supplementation on the quality of cryopreserved chicken semen. Biomed Res. Int. 2017:7279341. https://doi.org/10.1155/2017/7279341
Sangani, A. K., A. A. Masoudi, & R. V. Torshizi. 2017. Association of mitochondrial function and sperm progressivity in slow-and fast-growing roosters. Poult. Sci. 96:211-219. https://doi.org/10.3382/ps/pew273
Sayed, M. A. M., F. M. K. Abouelezz, & A. A. M. Abdel-Wahab. 2017. Analysis of sperm motility, velocity and morphometry of three Egyptian indigenous chicken strains. Egypt. Poult. Sci. J. 37:1173–1185. https://doi.org/10.21608/epsj.2017.5605
Setiawan, R., C. Priyadarshana, A. Tajima, A. J. Travis, & A. Asano. 2020. Localisation and function of glucose transporter GLUT1 in chicken (Gallus gallus domesticus) spermatozoa: Relationship between ATP production pathways and flagellar motility. Reprod. Fertil. Dev. 32:697-705. https://doi.org/10.1071/RD19240
Stanishevskaya, O., Y. Silyukova, N. Pleshanov, A. Kurochkin, E. Fedorova,Z. Fedorova, & I. Meftakh. 2021. Effects of saccharides supplementation in the extender of cryopreserved rooster (Gallus domesticus) semen on the fertility of frozen/thawed spermatozoa. Animals 11:189. https://doi.org/10.3390/ani11010189
Thélie, A., A. Bailliard, F. Seigneurin, T. Zerjal, M. Tixier-Boichard, & E. Blesbois. 2019. Chicken semen cryopreservation and use for the restoration of rare genetic resources. Poult. Sci. 98:447-455. https://doi.org/10.3382/ps/pey360
Telnoni, S. P., R. I. Arifiantini, & S. Darwati. 2021. Sperm fertility of SK Kedu chicken in lactated ringer’s-egg yolk extender with 10% of DMSO. International Journal Applied Biology 5:57-63.
Víquez, L., V. Barquero, C. Soler, E. R. Roldan, & A. Valverde. 2020. Kinematic sub-populations in bull spermatozoa: A comparison of classical and bayesian approaches. Biology 9:138. https://doi.org/10.3390/biology9060138
Woelders, H. 2021. Cryopreservation of Avian Semen. In: Wolkers, W. F. & H. Oldenhof (eds). Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology, vol 2180. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0783-1_16
Yamashiro, H., M. Toyomizu, M. Kikusato, N. Toyama, S. Sugimura, Y. Hoshino, & E. Sato. 2010. Lactate and adenosine triphosphate in the extender enhance the cryosurvival of rat epididymal sperm. J. Am. Assoc. Lab. Anim. Sci. 49:160-166.
Zhou, X., Y. Zhang, X. Wu, D. Wan, & Y. Yin. 2018. Effects of dietary serine supplementation on intestinal integrity, inflammation and oxidative status in early-weaned piglets. Cell. Physiol. Biochem. 48:993-1002. https://doi.org/10.1159/000491967
Authors
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors submitting manuscripts should understand and agree that copyright of manuscripts of the article shall be assigned/transferred to Tropical Animal Science Journal. The statement to release the copyright to Tropical Animal Science Journal is stated in Form A. This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA) where Authors and Readers can copy and redistribute the material in any medium or format, as well as remix, transform, and build upon the material for any purpose, but they must give appropriate credit (cite to the article or content), provide a link to the license, and indicate if changes were made. If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
Article Details
