Fatty Acid Biohydrogenation, Fermentation, and Digestibility of Ration Containing Napier and King Grass with Different Harvest Ages and Altitudes: In Vitro Study
Abstract
Forage is the primary and cheapest source of fatty acids (FA), which includes conjugated linoleic acid (CLA), influencing milk FA. This study aimed to analyze the fermentation, digestibility, biohydrogenation, nutrient composition, and FA content of napier grass (NG) and king grass (KG). Grasses were collected from the Pangalengan (highland) and Dramaga (lowland) districts at three harvest ages (1, 1.5, and 2 months). The feed was then analyzed for nutrients and FA. An in vitro study was performed to analyze the concentrations of NH3, VFA, protozoa populations, and biohydrogenation. No significant differences were observed in protozoa, pH, total VFA, or FA biohydrogenation. NH3 ranged from 5.31 mM to 8.86 mM. Significant differences were found at different altitudes, with an interaction between grass type and harvest age and an interaction between the three factors. The highest NH3 concentration was found in rations containing highland NG at 1.5 months. The DMD value was 58.27%–64.39%, and OMD was 61.07%–67.18%. Different digestibility values were observed at different harvest ages, with an interaction between altitude and harvest age. This aligned with the CF, NDF, and lignin contents in grasses. The highest was at 1.5 months NG. Significant differences were observed in the relative proportions of propionic acids. The highest value was in the ration containing the 1.5-month highland NG. Rations containing KG yielded significantly higher amounts of the C18:0 and C18:1 trans. In conclusion, the 1.5-month highland NG is a potential ration for supporting healthier FA production in milk.
References
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Makmur, M., M. Zain, Y. Marlida, Khasrad, & A. Jayanegara. 2019. Fatty acids composition and biohydrogenation reduction agents of tropical forages. Biodiversitas 20:1917–1922. https://doi.org/10.13057/biodiv/d200718
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Nadeau, E., D. O. de Sousa D, A. Magnusson, S. Hedlund, W. Richardt, & P. Nørgaard. 2019. Digestibility and protein utilization in wethers fed whole-crop barley or grass silages harvested at different maturity stages, with or without protein supplementation. J. Anim. Sci. 97:2188–2201. https://doi.org/10.1093/jas/skz076
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NRC. 2001. Nutrient Requirements of Dairy Cattle: Seventh Revised Edition. Seventh Re. National Academy Press, Washington DC.
Ogimoto, K. & S. Imai. 1981. Atlas of Rumen Microbiology. Japan Scientific Societies Press, Tokyo.
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Putri, E. M., M. Zain, L. Warly, & H. Hermon. 2021. Effects of rumen-degradable-to-undegradable protein ratio in ruminant diet on in vitro digestibility, rumen fermentation, and microbial protein synthesis. Vet. World 14:640–648. https://doi.org/10.14202/vetworld.2021.640-648
Raffrenato, E., R. Fievisohn, K. W. Cotanch, R. J. Grant, L. E. Chase, & M. E. Van Amburgh. 2017. Effect of lignin linkages with other plant cell wall components on in vitro and in vivo neutral detergent fiber digestibility and rate of digestion of grass forages. J. Dairy Sci. 100:8119–8131. https://doi.org/10.3168/jds.2016-12364
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Authors
AnzhanyD., ToharmatT., Despal, & Łozicki A. (2024). Fatty Acid Biohydrogenation, Fermentation, and Digestibility of Ration Containing Napier and King Grass with Different Harvest Ages and Altitudes: In Vitro Study. Tropical Animal Science Journal, 47(1), 68-78. https://doi.org/10.5398/tasj.2024.47.1.68
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