Genetic Association and Expression of JHDM1A Gene Related to Meat pH in Commercial Pigs
Abstract
An experiment was conducted to study the association and expression of JHDM1A gene as a candidate gene for meat quality. The polymorphism was genotyped by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using restriction enzyme on a total of 300 muscle samples of [Duroc × (Large White × Landrace)] pigs. Results showed that JHDM1A gene was significantly associated with meat pH 45 min post-mortem (p.m.) (p<0.05). Allele frequencies for G and C were 0.53 and 0.47. The genotype frequencies for GG, GC, and CC were 0.24, 0.58, and 0.18, respectively. The Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) study were analyzed between low and high pH 45 min p.m. groups (n=10 per group) according to the association result. JHDM1A expression was higher in animals with a low post-mortem meat pH 45 min (p<0.05). Therefore, polymorphism and expression in the porcine JHDM1A gene might be the important candidate genes to improve meat quality traits in terms of meat pH.
References
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Kayan, A., M. J. Uddin, H. Kocamis, D. Tesfaye, C. Looft, E. Tholen, K. Schellander, & M. U. Cinar. 2013. Association and expression analysis of porcine HNF1A gene related to meat and carcass quality traits. Meat Sci. 94:474-479. https://doi.org/10.1016/j.meatsci.2013.04.015
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Le, T. T., H. T. Nguyen, & M. A. Pham. 2020. Rigor mortis development and effects of filleting conditions on the quality of Tra catfish (Pangasius hypophthalmus) fillets. J. Food Sci. Technol. 57:1320-1330. https://doi.org/10.1007/s13197-019-04166-x
Liu, G., D. Jennen, & E. Tholen. 2007. A genome scan reveals QTL for growth, fatness, leanness and meat quality in a Duroc-Pietrain resource population. Anim. Genet. 38:241-252. https://doi.org/10.1111/j.1365-2052.2007.01592.x
Liu, J., M. Pan, D. Huang, J. Wu, Y. Liu, Y. Guo, W. Zhang, & K. Mai. 2021. High glucose induces apoptosis, glycogen accumulation and suppresses protein synthesis in muscle cells of olive flounder Paralichthys olivaceus. Br. J. Nutr. 1-12. https://doi.org/10.1017/S0007114521002634
Mateescu, R. G., D. J. Garrick, & J. M. Reecy. 2017. Network analysis reveals putative genes affecting meat quality in Angus cattle. Front. Genet. 8:171. https://doi.org/10.3389/fgene.2017.00171
Moreno, I., P. Lipová, L. Ladero, J. L. Fernández-García, & R. Cava. 2020. Glycogen and lactate contents, pH and meat quality and gene expression in muscle Longissimus dorsi from iberian pigs under different rearing conditions. Livest. Sci. 240:104167. https://doi.org/10.1016/j.livsci.2020.104167
Nilsson, C., U. Johansson, A. C. Johansson, K. Kagedal, & K. Ollinger. 2006. Cytosolic acidification and lysosomal alkalinization during TNF-alpha induced apoptosis in U937 cells. Apoptosis. 11:1149–1159. https://doi.org/10.1007/s10495-006-7108-5
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Pahuja, S., S. Jain, M. Nain, R. Goel, S. Sehgal, & M. Jain. 2020. Assessment of rhesus and kell blood group antigens, phenotypes, and their allelic frequencies in North Indian blood donors. Asian J. Transfus. Sci. 14:137. https://doi.org/10.4103/ajts.AJTS_9_19
Pegolo, S., A. Cecchinato, S. Savoia, L. Di Stasio, A. Pauciullo, A. Brugiapaglia, G. Bittante, & A. Albera. 2020. Genome-wide association and pathway analysis of carcass and meat quality traits in Piemontese young bulls. Animal. 14:243-252. https://doi.org/10.1017/S1751731119001812
Peng, Y. B., B. Fan, & X. l. Han. 2011. Molecular characterization of the porcine JHDM1A gene associated with average daily gain: evaluation its role in skeletal muscle development and growth. Mol. Biol. Rep. 38:4697-4704. https://doi.org/10.1007/s11033-010-0604-2
Piórkowska, K., K. Żukowski, K. Ropka-Molik, M. Tyra, & A. Gurgul. 2018. A comprehensive transcriptome analysis of skeletal muscles in two Polish pig breeds differing in fat and meat quality traits. Genetics and Molecular Biology 41:125-136. https://doi.org/10.1590/1678-4685-gmb-2016-0101
Plastow, G. & H. Bruce. 2014. Modern Genetics and Genomic Technologies and their Application in the Meat Industry-Red Meat Animal, Poultry. In: M. Dikeman, & C. Devine (Editors). Encyclopedia of Meat Sciences 2nd ed. Academic Press, UK, p. 37–42. https://doi.org/10.1016/B978-0-12-384731-7.00189-6
Savenije, B., E. Lambooij, M. Gerritzen, K. Venema, & J. Korf. 2002. Effects of feed deprivation and transport on preslaughter blood metabolites, early postmortem muscle metabolites, and meat quality. Poult. Sci. 81:699-708. https://doi.org/10.1093/ps/81.5.699
Scheffler, T. & D. Gerrard. 2007. Mechanisms controlling pork quality development: The biochemistry controlling postmortem energy metabolism. Meat Sci. 77:7-16. https://doi.org/10.1016/j.meatsci.2007.04.024
Zhang, X., S. Yang, J. Chen, & Z. Su. 2019. Unraveling the regulation of hepatic gluconeogenesis. Front. Endocrinol. 9:802. https://doi.org/10.3389/fendo.2018.00802
Campos, G. S., B. P. Sollero, F. A. Reimann, V. S. Junqueira, L. L. Cardoso, M. J. I. Yokoo, A. A. Boligon, J. Braccini, & F. F. Cardoso. 2020. Tag‐SNP selection using Bayesian genomewide association study for growth traits in Hereford and Braford cattle. J. Anim. Breed. Genet. 137:449-467. https://doi.org/10.1111/jbg.12458
Choe, J., Y. Choi, & S. Lee. 2009. The relation of blood glucose level to muscle fiber characteristics and pork quality traits. Meat Sci. 83:62-67. https://doi.org/10.1016/j.meatsci.2009.03.011
Daskalova, A. 2019. Farmed fish welfare: stress, post-mortem muscle metabolism, and stress-related meat quality changes. Int. Aquat. Res. 11:113-124. https://doi.org/10.1007/s40071-019-0230-0
Davoli, R. & S. Braglia. 2007. Molecular approaches in pig breeding to improve meat quality. Brief. Funct. Genomics. 6:313-321. https://doi.org/10.1093/bfgp/elm036
Davoli, R., C. Schivazappa, P. Zambonelli, S. Braglia, A. Rossi, & R. Virgili. 2017. Association study between single nucleotide polymorphisms in porcine genes and pork quality traits for fresh consumption and processing into Italian dry cured ham. Meat Sci. 126:73-81. https://doi.org/10.1016/j.meatsci.2016.11.018
Fukuda, T., A. Tokunaga, R. Sakamoto, & N. Yoshida. 2011. FbxI10/Kdm2b deficiency accelerates neural progenitor cell death and leads to exencephaly. Mol. Cell. Neurosci. 46:614–624. https://doi.org/10.1016/j.mcn.2011.01.001
Gao, Y., R. Zhang, X. Hu, & N. Li. 2007. Application of genomic technologies to the improvement of meat quality of farm animals. Meat Sci. 77:36-45. https://doi.org/10.1016/j.meatsci.2007.03.026
Gao, Y., J. Zhang, L. He, X. Shi, L. Han, Q. Yu, Y. Yang, R. Song, M. Han, & S. Zhao. 2020. Associations among adenosine monophosphate-activated protein kinase, glycolysis, muscle characteristics, and apoptosis in postmortem bovines longissimus muscle. Eur. Food Res. Technol. 246:971-985. https://doi.org/10.1007/s00217-020-03458-3
Gebreselassie, G., H. Berihulay, L. Jiang, & Y. Ma. 2020. Review on genomic regions and candidate genes associated with economically important production and reproduction traits in sheep (Ovies aries). Animals. 10: 33. https://doi.org/10.3390/ani10010033
Große-Brinkhaus, C., E. Jonas, & H. Buschbell. 2010. Epistatic QTL pairs associated with meat quality and carcass composition traits in a porcine Duroc × Pietrain population. Genet. Sel. Evol. 42:39. https://doi.org/10.1186/1297-9686-42-39
Han, X., H. Yang, & T. Jiang. 2014. Investigation of four candidate genes (IGF2, JHDM1A, COPB1 and TEF1) for growth rate and backfat thickness traits on SSC2q in large white pigs. Mol. Biol. Rep. 41:309-315. https://doi.org/10.1007/s11033-013-2863-1
He, J., E. Kallin, Y. I. Tsukada, & Y. Zhang. 2008. The H3K36 demethylase Jhdm1b/Kdm2b regulates cell proliferation and senescence through p15Ink4b. Nat. Struct. Mol. Biol. 15:1169-1175. https://doi.org/10.1038/nsmb.1499
Honikel, K., C. Kim, & R. Hamm. 1986. Sarcomere shortening of prerigor muscles and its influence on drip loss. Meat Sci. 16:267-282. https://doi.org/10.1016/0309-1740(86)90038-0
Jennen, D. G. J., A. D. Brings, G. Liu, H. Jungst, E. Tholen, E. Jonas, D. Tesfaye, K. Schellander, & C. Phatsara. 2007. Genetic aspects concerning drip loss and water-holding capacity of porcine meat: Review Article. J. Anim. Breed Genet. 124:2–11. https://doi.org/10.1111/j.1439-0388.2007.00681.x
Kawakami, E., A. Tokunaga, M. Ozawa, R. Sakamoto, & N. Yoshida. 2015. The histone demethylase Fbxl11/Kdm2a plays an essential role in embryonic development by repressing cell-cycle regulators. Mech. Dev. 135:31–42. https://doi.org/10.1016/j.mod.2014.10.001
Kayan, A., M. J. Uddin, H. Kocamis, D. Tesfaye, C. Looft, E. Tholen, K. Schellander, & M. U. Cinar. 2013. Association and expression analysis of porcine HNF1A gene related to meat and carcass quality traits. Meat Sci. 94:474-479. https://doi.org/10.1016/j.meatsci.2013.04.015
Koomkrong, N., C. Boonkaewwan, W. Laenoi, & A. Kayan. 2017. Blood haematology, muscle pH and serum cortisol changes in pigs with different levels of drip loss. Asian-Australas. J. Anim. Sci. 30:1751. https://doi.org/10.5713/ajas.17.0037
Le, T. T., H. T. Nguyen, & M. A. Pham. 2020. Rigor mortis development and effects of filleting conditions on the quality of Tra catfish (Pangasius hypophthalmus) fillets. J. Food Sci. Technol. 57:1320-1330. https://doi.org/10.1007/s13197-019-04166-x
Liu, G., D. Jennen, & E. Tholen. 2007. A genome scan reveals QTL for growth, fatness, leanness and meat quality in a Duroc-Pietrain resource population. Anim. Genet. 38:241-252. https://doi.org/10.1111/j.1365-2052.2007.01592.x
Liu, J., M. Pan, D. Huang, J. Wu, Y. Liu, Y. Guo, W. Zhang, & K. Mai. 2021. High glucose induces apoptosis, glycogen accumulation and suppresses protein synthesis in muscle cells of olive flounder Paralichthys olivaceus. Br. J. Nutr. 1-12. https://doi.org/10.1017/S0007114521002634
Mateescu, R. G., D. J. Garrick, & J. M. Reecy. 2017. Network analysis reveals putative genes affecting meat quality in Angus cattle. Front. Genet. 8:171. https://doi.org/10.3389/fgene.2017.00171
Moreno, I., P. Lipová, L. Ladero, J. L. Fernández-García, & R. Cava. 2020. Glycogen and lactate contents, pH and meat quality and gene expression in muscle Longissimus dorsi from iberian pigs under different rearing conditions. Livest. Sci. 240:104167. https://doi.org/10.1016/j.livsci.2020.104167
Nilsson, C., U. Johansson, A. C. Johansson, K. Kagedal, & K. Ollinger. 2006. Cytosolic acidification and lysosomal alkalinization during TNF-alpha induced apoptosis in U937 cells. Apoptosis. 11:1149–1159. https://doi.org/10.1007/s10495-006-7108-5
Oskoueian, E., W. Mullen, & A. Albalat. 2016. Proteomic Application for Farm Animal Management. In: G.H. Salekden (editor). Agricutural Proteomic. Springer International Publishing, Switzerland. p. 147–163. https://doi.org/10.1007/978-3-319-43275-5_9
Pahuja, S., S. Jain, M. Nain, R. Goel, S. Sehgal, & M. Jain. 2020. Assessment of rhesus and kell blood group antigens, phenotypes, and their allelic frequencies in North Indian blood donors. Asian J. Transfus. Sci. 14:137. https://doi.org/10.4103/ajts.AJTS_9_19
Pegolo, S., A. Cecchinato, S. Savoia, L. Di Stasio, A. Pauciullo, A. Brugiapaglia, G. Bittante, & A. Albera. 2020. Genome-wide association and pathway analysis of carcass and meat quality traits in Piemontese young bulls. Animal. 14:243-252. https://doi.org/10.1017/S1751731119001812
Peng, Y. B., B. Fan, & X. l. Han. 2011. Molecular characterization of the porcine JHDM1A gene associated with average daily gain: evaluation its role in skeletal muscle development and growth. Mol. Biol. Rep. 38:4697-4704. https://doi.org/10.1007/s11033-010-0604-2
Piórkowska, K., K. Żukowski, K. Ropka-Molik, M. Tyra, & A. Gurgul. 2018. A comprehensive transcriptome analysis of skeletal muscles in two Polish pig breeds differing in fat and meat quality traits. Genetics and Molecular Biology 41:125-136. https://doi.org/10.1590/1678-4685-gmb-2016-0101
Plastow, G. & H. Bruce. 2014. Modern Genetics and Genomic Technologies and their Application in the Meat Industry-Red Meat Animal, Poultry. In: M. Dikeman, & C. Devine (Editors). Encyclopedia of Meat Sciences 2nd ed. Academic Press, UK, p. 37–42. https://doi.org/10.1016/B978-0-12-384731-7.00189-6
Savenije, B., E. Lambooij, M. Gerritzen, K. Venema, & J. Korf. 2002. Effects of feed deprivation and transport on preslaughter blood metabolites, early postmortem muscle metabolites, and meat quality. Poult. Sci. 81:699-708. https://doi.org/10.1093/ps/81.5.699
Scheffler, T. & D. Gerrard. 2007. Mechanisms controlling pork quality development: The biochemistry controlling postmortem energy metabolism. Meat Sci. 77:7-16. https://doi.org/10.1016/j.meatsci.2007.04.024
Zhang, X., S. Yang, J. Chen, & Z. Su. 2019. Unraveling the regulation of hepatic gluconeogenesis. Front. Endocrinol. 9:802. https://doi.org/10.3389/fendo.2018.00802
Authors
KayanA., TheerawatanasirikulS., LekcharoensukP., BoonkaewwanC., KaewkotA., ChanaksornM., TantikositrujC., & GunawanA. (2022). Genetic Association and Expression of JHDM1A Gene Related to Meat pH in Commercial Pigs. Tropical Animal Science Journal, 45(2), 128-133. https://doi.org/10.5398/tasj.2022.45.2.128
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