Maintaining the Quality of Kacang Buck Semen in Chilled Storage with the Addition of Green Tea Extract in Extender

  • S. Susilowati Faculty of Veterinary Medicine, Universitas Airlangga
  • I. Mustofa Faculty of Veterinary Medicine, Universitas Airlangga
  • W. Wurlina Faculty of Veterinary Medicine, Universitas Airlangga
  • T. Hernawati Faculty of Veterinary Medicine, Universitas Airlangga
  • Y. Oktanella Faculty of Veterinary Medicine, Brawijaya University
Keywords: DNA fragmentation, intact plasma membrane, malondialdehyde, sperm motility, poverty reduction

Abstract

Kacang goats can be bred through artificial insemination techniques using liquid semen from a superior buck. The study was aimed to determine the quality of Kacang buck semen in chilled storage when green tea extract (GTE) was added to the semen extender. Specifically, 12 ejaculates from three Kacang bucks were diluted in skim milk–egg yolk extender containing 0 mg, 0.05, 0.10, and 0.15 mg of GTE/100 mL. The extended semen was stored at 5°C, and its quality was evaluated daily for 5 d. Comparatively, semen with 0.10 mg of GTE/100 mL had the best quality (p<0.05): it had the highest sperm viability, sperm progressive motility, and sperm intact plasma membrane values, and the lowest malondialdehyde levels and DNA fragmentation percentage. However, as the storage period increased, there was a decrease in sperm viability, sperm motility, and sperm intact plasma membrane values with an increase in malondialdehyde levels and DNA fragmentation (p<0.05). The sperm motility of semen with 0.10 mg of GTE/100 mL was maintained during 5 d of chilled storage to meet the qualification for artificial insemination. In contrast, the sperm motility of semen with 0.05 mg and 0.15 mg of GTE/100 mL was maintained for 4 d, whereas that of the control semen was maintained only for 3 d. Thus, the addition 0.10 mg of GTE/100 mL of skim milk-egg yolk extender seems to help maintain the quality of Kacang goat sperm in chilled storage.

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References

Allai, L., A. Benmoula, M. M. Marciane da Silva, B. Nasser, & B. El Amiri. 2018. Supplementation of ram semen extender to improve seminal quality and fertility rate. Anim. Reprod. Sci. 192:6-17. https://doi.org/10.1016/j.anireprosci.2018.03.019

Anzar, M., K. Rajapaksha, & L. Boswall. 2019. Egg yolk-free cryopreservation of bull semen. PLOS ONE 14:e0223977. https://doi.org/10.1371/journal.pone.0223977

Di Iorio, M., A. Manchisi, M. Rocco, P. Chrenek, & N. Iaffaldano. 2014. Comparison of different extenders on the preservability of rabbit semen stored at 5°C for 72 hours. Ital. J. Anim. Sci. 13:3444. https://doi.org/10.4081/ijas.2014.3444

Dwinofanto, H., R. Rimayanti, I. Mustofa, S. Susilowati, & T. Hernawati. 2018. The effect of duration of preservation on the quality, MDA level, and DNA damage of post-thawed Bali cattle bull sperm. Iraqi J. Vet. Sci. 32:249-252. https://doi.org/10.33899/ijvs.2019.153857

El-Battawy, K. A. 2019. Preservation of goat semen at 5°C with emphasis on its freezability and the impact of melatonin. Int. J. Vet. Sci. Res. 5:035-038. https://doi.org/10.17352/ijvsr.000039

Ezz, M. A., A. E. Montasser, M. Hussein, A. Eldesouky, M. Badr, A. E. Hegab, A. Balboula, & S. M. Zaabel. 2017. The effect of cholesterol loaded cyclodextrins on post-thawing quality of buffalo semen in relation to sperm DNA damage and ultrastructure. Reprod. Biol. 17:42-50. https://doi.org/10.1016/j.repbio.2016.12.001

Fadl, A. M., A. M. Ghallab, & M. M. Abou-Ahmed. 2020. Comparison between Tris-buffer and INRA-82 extenders on the quality of chilled rabbit spermatozoa. World Rabbit Sci. 28:13-18. https://doi.org/10.4995/wrs.2020.12287

Ferdinand, N., T. T. Thomas, K. Augustave, D. F. Henry, T. Fernand, & P. T. Etienne. 2012. Effects of goat age, storage duration, storage temperature and diluent on fresh West African Dwarf goat semen. J. Reprod. Infertil. 3:58-66. https://doi.org/10.5829/idosi.jri.2012.3.3.66216

Hahn, K., K. Failing, & A. Wehrend. 2019. Effect of temperature and time after collection on goat semen quality. BMC Vet. Res. 15:355. https://doi.org/10.1186/s12917-019-2135-y

Kumar, A., J. K. Prasad, N. Srivastava, & S. K. Ghosh. 2019. Strategies to minimize various stress-related freeze-thaw damages during conventional cryopreservation of mammalian spermatozoa. Biopreserv. Biobank. 17:603-612. https://doi.org/10.1089/bio.2019.0037

Kusumawati, E. D., K. N. Utomo, A. T. N. Krisnaningsih, & S. Rahadi. 2017. Kualitas semen kambing Kacang dengan lama simpan yang berbeda pada suhu ruang menggunakan pengencer tris aminomethan kuning telur. JITRO 4:42-51. https://doi.org/10.33772/jitro.v4i3

Leite, T. G., V. R. de Vale Filhoa, R. P. de Arrudab, A. F. C. de Andradeb, L.L. Emericka, F. G. Zaffalonb, J. A. M. Martinsa, & V. J. Andrade. 2010. Effects of extender and equilibration periodon post-thaw motility and membrane integrity of cryopreserved Gyr bull semen evaluated by CASA and flow cytometry. Anim. Reprod. Sci. 120:31-38. https://doi.org/10.1016/j.anireprosci.2010.04.005

Macías, A., L. M. Ferrer, J. J. Ramos, I. Lidón, R. Rebollar, D. Lacasta, & M. T. Tejedor. 2017. Technical Note: A new device for cervical insemination of sheep - design and field test. J. Anim. Sci. 95:5263-5269. https://doi.org/10.2527/jas2017.1951

Majzoub, A. & A. Agarwal. 2018. Systematic review of antioxidant types and doses in male infertility: benefits on semen parameters, advanced sperm function, assisted reproduction and live-birth rate. Arab J. Urol. 16:113-124. https://doi.org/10.1016/j.aju.2017.11.013

Majzoub, A., A. Agarwal, & S. C. Esteves. 2017. Antioxidants for elevated sperm DNA fragmentation: a mini review. Transl. Androl. Urol. 6 (Suppl 4):S649-S653. https://doi.org/10.21037/tau.2017.07.09

Manente, L., S. Pecoraro, E. Picillo, U. Gargiulo, P. Gargiulo, A. De Luca, & L. Politano. 2015. Molecular evidence of apoptotic pathway activation in semen samples with high DNA fragmentation. In vivo 29: 289-294.

Martin-Hidalgo, D., M. J. Bragado, A. R. Batista, P. F. Oliveira, & M. G. Alves. 2019. Antioxidants and male fertility: From molecular studies to clinical evidence. Antioxidants 8: 89: 1-21. https://doi.org/10.3390/antiox8040089

Mehdipour, M., H. D. Kia, A. Najafi, H.V. Dodaran, & O. García-Álvarez. 2016. Effect of green tea (Camellia sinensis) extract and pre-freezing equilibration periodon the post-thawing quality of ram semen cryopreserved in a soybean lecithin based extender. Cryobiology 73:297-303. https://doi.org/10.1016/j.cryobiol.2016.10.008

Rahman, M. S., M. R. Gofur, M. M. Rahman, F. Y. Bari, & N. S. Juyena. 2018. Effect of skim milk and tris-citrate extenders to preserve the semen of indigenous ram of Bangladesh. AJOB 5:1-11. https://doi.org/10.9734/AJOB/2018/39448

Reynolds, S., N. F. B. Ismail, S. J. Calvert, A. A. Pacey, & M. N. J. Paley. 2017. Evidence for rapid oxidative phosphorylation and lactate fermentation in motile human sperm by hyperpolarized 13C magnetic resonance spectroscopy. Sci. Rep. 7:4322. https://doi.org/10.1038/s41598-017-04146-1

Sanocka, D. & M. Kurpisz. 2004. Reactive oxygen species and sperm cells. Reprod. Biol. Endocrinol. 2: 1-7. https://doi.org/10.1186/1477-7827-2-12

Setiawan, F. & E. D. Kusumawati. 2017. The quality of fresh semen Goat at 5°C storage with and without an extender. J. Anim. Sci. 95:77-85. https://doi.org/10.21067/jsp.v5i2.3157

Standar Nasional Indonesia (SNI) 4869.3. 2014. Semen beku – Bagian 3 : Kambing dan domba. Badan Standardisasi Nasional Indonesia. Retrieved from: http://bibit.ditjenpkh.pertanian.go.id/sites/default/files/SNI%204869.3-2014%20Semen%20beku%2C%20Bagian%203%20Kambing%20dan%20domba%20%20%2001.pdf [June 23, 2020].

Susilowati, S., I. N. Triana, T. Sardjito, T. W. Suprayogi, W. Wurlina, & I. Mustofa. 2020. Effect of Simmental bull seminal plasma protein in egg yolk-citrate extender on Kacang buck semen fertility. Cryobiology 97:20-27. https://doi.org/10.1016/j.cryobiol.2020.10.013

Susilowati, S., I. N. Triana, W. Wurlina, A. Arimbi, P. Srianto, & I. Mustofa. 2019. Supplementation of L-arginine in skim milk extender maintains goat semen quality in chilled temperature for five days. Vet. World. 12:1784-1789. https://doi.org/10.14202/vetworld.2019.1784-1789

Susilowati, S., T. Sardjito, I. Mustofa, O. S. Widodo, & R. Kurnijasanti. 2021. Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients. AJAS. Anim. Biosci. 34:198-204. https://doi.org/10.5713/ajas.20.0025

Takeda, K., K. Uchiyama, M. Kinukawa, T. Tagami, M. Kaneda, & S. Watanabe. 2015. Evaluation of sperm DNA damage in bulls by TUNEL assay as a parameter of semen quality. J. Reprod. Dev. 61:185-190. https://doi.org/10.1262/jrd.2014-140

Takeshima, T., S. Kuroda, & Y. Yumura. 2018. Chapter 6: Reactive Oxygen Species and Sperm Cells. https://doi.org/10.5772/intechopen.73037

Thompson, A., A. Agarwal, & S. S. Du Plessis. 2014. The Physiological Role of Reactive Oxygen Species in Sperm Function: A review. In: Parekattil, S. J. & A. Agarwal (Eds). Antioxidants in Male Infertility: A Guide for Clinicians and Researchers. Springer Science+Business Media, New York. https://doi.org/10.1007/978-1-4614-9158-3_4

Ugur, M. R., T. Dinh, M. Hitit, M., A. Kaya, E. Topper, B. Didion, & E. Memili. 2020. Amino acids of seminal plasma associated with freezability of bull sperm. Front. Cell Dev. Biol. 7: 347. https://doi.org/10.3389/fcell.2019.00347

Wagner, H., J. W. Cheng, & E. Y. Ko. 2018. Role of reactive oxygen species in male infertility: An updated review of the literature. Arab J. Urol. 16:35-43. https://doi.org/10.1016/j.aju.2017.11.001

Wen, F., Y. Li, T. Feng, Y. Du, F. Ren, L. Zhang, N. Han, S. Ma, F. Li, P. Wang, & J. Hu. 2019. Grape seed procyanidin extract (GSPE) improves goat semen quality when preserved at 4°C. Animals 9:1-11. https://doi.org/10.3390/ani9100810

Wusiman, A. Y. P. W., K. Ren, G. B. Zhou, X. W. Fu, L. Suo, Z. Q. Fan, L. Wang, & S. E. Zhu. 2012. Semen storage at 23, 4 or −196°C and its application to artificial insemination in Small-tail Han Sheep. AJAVA 7:299-308. https://doi.org/10.3923/ajava.2012.299.308

Zhang, Y., H. Lin, C. Liu, J. Huang, & Z. Liu. 2020. A review for physiological activities of EGCG and the role in improving fertility in humans/mammals. Biomed. Pharmacother. 127:1-10 (110186). https://doi.org/10.1016/j.biopha.2020.110186

Published
2021-11-12
How to Cite
SusilowatiS., MustofaI., WurlinaW., HernawatiT., & OktanellaY. (2021). Maintaining the Quality of Kacang Buck Semen in Chilled Storage with the Addition of Green Tea Extract in Extender. Tropical Animal Science Journal, 44(4), 408-414. https://doi.org/10.5398/tasj.2021.44.4.408