The Protective Impacts of α-tocopherol Supplementation on the Semen Quality of Sapera Goat Preserved at 4⁰C
Abstract
Supplementation of vitamin E (α-tocopherol) as an antioxidant into an extender is useful to maintain sperm quality during the process of cryopreservation by protecting the sperms from reactive oxygen species (ROS). This study aims to evaluate the protective impacts of vitamin E supplementation on the motility, viability, and reducing the abnormality of sperms in Sapera goats. The sample of semen used in the present study was obtained from fresh semen and good-quality ejaculates of the Sapera goats collected by an artificial vagina. The groups involve one controlled treatment group, which contains semen and tris egg yolk (TEY) extender without vitamin E (P0), and three treatment groups that contain semen, TEY extender, and different doses of vitamin E, i.e., 1 mg/mL (P1), 2 mg/mL (P2), and 3 mg/mL (P3), respectively. Data obtained from the experiments were analyzed using Repeated Measures Multivariate Analysis (MANOVA). The results showed that TEY extenders added with vitamin E at a dose of 1 mg/mL of vitamin E (P1) produced the highest motility and viability with the lowest abnormalities of sperm after 24th hour of cryopreservation. The lowest sperm motility and viability among the treatment groups was observed in the TEY extender added with vitamin E at a dose of 3 mg/mL (P3) at 96th hour after cryopreservation, which was almost the same as the control group without vitamin E (P0) that had the lowest motility and viability. In conclusion, the current study found that supplementation of vitamin E as an antioxidant at a dose of 1 mg/mL in TEY extender can preserve semen of the Sapera goats in refrigerator up to 96 h at 4°C and provides higher sperm motility and viability percentage, with a lower percentage of sperm abnormality.
References
Aitken, R. J. 2017. Reactive oxygen species as mediators of sperm capacitation and pathological damage. Mol. Reprod. Dev. 84:1039–1052. https://doi.org/10.1002/mrd.22871
Am-In, N., R. Kirkwood, M. Techakumphu, & W. Tantasuparuk. 2011. Lipid profiles of sperm and seminal plasma from boars having normal or low sperm motility. Theriogenology 75:897-903. https://doi.org/10.1016/j.theriogenology.2010.10.032
Am-In, N., R. Kirkwood, M. Techakumphu, & W. Tantasuparuk. 2010. Effect of storage for 24 h at 18°C on sperm quality and a comparison of two assays for sperm membrane lipid peroxidation. Can. J. Anim. Sci. 90:389-392. https://doi.org/10.4141/CJAS09122
Azzawi, O. I. and E. K. Hussein. 2013. Effect of vitamins C or E supplementation to tris diluent on the semen quality of Awassi rams preserved at 5°C. Vet. Res. Forum. 4:157-160.
Azzi, A. 2007. Molecular mechanism of alpha-tocopherol action. Free. Radic. Biol. Med. 43:16-21. https://doi.org/10.1016/j.freeradbiomed.2007.03.013
Breininger, E., N. B. Beorlegui, C. M. O’Flaherty, & M. T. Beconi. 2005. Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen. Theriogenology 63:2126-2135. https://doi.org/10.1016/j.theriogenology.2004.08.016
Cseh, S., V. Faigl, & G. S. Amiridis. 2012. Amiridis, semen processing and artificial insemination in health management of small ruminants. Anim. Reprod. Sci. 130:187-192. https://doi.org/10.1016/j.anireprosci.2012.01.014
Faigl, V., N. Vass, A. Javor, M. Kulcsar, L. Solti, G. Amiridis, & S. Cseh. 2012. Artificial insemination of small ruminants: A review. Acta Vet. Hung. 60: 115-129. https://doi.org/10.1556/AVet.2012.010
Fang, Q., J. Wang, Y. Y. Hao, H. Li, J. X. Hu, & G. S. Yang. 2017. Effects of iodine methionine on boar sperm quality during liquid storage at 17oC. Reprod. Domest. Anim. 52: 1061–1066. https://doi.org/10.1111/rda.13024
Felipe-Perez, Y. E., M. L. Juarez-Mosqueda, E. O. Hernandez-Gonzalez, & J. J. Valencia. 2008. Viability of fresh and frozen bull sperm compared by two staining techniques. Acta Vet. Bras. 2:123-130.
Forouzanfar, M., A. Abid, S. M. Hosseini, M. Hajian, & M. H. N. Esfahani. 2013a. Supplementation of sperm cryopreservation media with cell permeable superoxide dismutase mimetic agent (MnTE) improves goat blastocyst formation. Cryobiology. 67:394-397. https://doi.org/10.1016/j.cryobiol.2013.08.004
Forouzanfar, M., S. F. Ershad, S. M. Hosseini, M. Hajian, S. Ostad-Hosseini, A. Abid, M. Tavalaee, A. Shahverdi, A. V. Dizaji, & M. H. N. Esfahani. 2013b. Can permeable super oxide dismutase mimetic agents improve the quality of frozen-thawed ram semen?. Cryobiology 66:126-130. https://doi.org/10.1016/j.cryobiol.2012.12.006
Gibb Z. & R. J. Aitken. 2016. The impact of sperm metabolism during in vitro storage: the stallion as a model. Biomed Res. Int. 2016:1-8. https://doi.org/10.1155/2016/9380609
Hadi, I. H. 2016. Effect of vitamin E on sperm motility and survival in chilled-stored semen. Iraqi Journal of Embryo and Infertility Researches. 6:1-7.
Hajibabaei, K. 2016. Antioxidant properties of vitamin E. Ann. Res. Antioxid. 1:e22.
Halliwell, B. & J. M. C. Gutteridge. 2015. Free radicals in biology and medicine, 5th ed. Oxford, Oxford University Press.
Hardijanto, S. Susilowati, T. Hernawati, T. Sardjito, T. W. Suprayogi. 2010. Buku Ajar Inseminasi Buatan. 1st ed. Pusat Penerbitan dan Percetakan Unair, Surabaya.
Herdis, H., I. Kusuma, & D. I. W. Angga. 2013. Pengaruh penambahan a-tokoferol pada media pengencer tris kuning telur terhadap kualitas semen cair domba garut (Indonesian). Jurnal Sains dan Teknologi Indonesia. 11:175-180. https://doi.org/10.29122/jsti.v11i3.836
Johnston, S. D., N. Satake, Y. Zee, C. Lopez-Fernandez, W.V. Holt, & J. Gosalvez. 2012. Osmotic stress and cryoinjury of koala sperm: An integrative study of the plasma membrane, chromatin stability and mitochondrial function. Reproduction. 143:787-797. https://doi.org/10.1530/REP-11-0436
Khorramabadi, K. M., A. A. Sarcheshmeh, A. R. Talebi, & A. Mirjalili. 2019. Protective effect of vitamin E on oxidative stress and sperm apoptosis in diabetic mice. Int. J. Reprod. Biomed. 17:127-134. https://doi.org/10.18502/ijrm.v17i2.3990
Kim, S., C. Agca, & Y. Agca. 2012. Changes in rat sperm function after cooling, cryopreservation and centrifugation processes. Cryobiology. 65:215-223. https://doi.org/10.1016/j.cryobiol.2012.06.006
Liu T., Y. Han, T. Zhou, R. Zhang, H. Chen, S. Chen, & H. Zhao. 2019. Mechanisms of ROS-induced mitochondria dependent apoptosis underlying liquid storage of goat spermatozoa. Aging. 18:7880-7898. https://doi.org/10.18632/aging.102295
Maya, W., K. Mayur, & S. Ashar. 2012. Pharmaceutical profile of alpha-tocopherol: A brief review. Int. J. Pharm. Chem. Sci. 1:1023-1039
Najafi, A., M. Zhandi, A. Towhidi, M. Sharafi, A. A. Sharif, M. K. Motlagh, & F. Martinez-Pastor. 2013. Trehalose and glycerol have a dose-dependent synergistic effect on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology 66:275-282. https://doi.org/10.1016/j.cryobiol.2013.03.002
Noori, S. 2012. An overview of oxidative stress and antioxidant defensive system. J. Clin. Cell. Immunol. 1:1-9. https://doi.org/10.4172/scientificreports.413
O’Connell, M., N. McClure, & S. E. Lewis. 2002. The effects of cryopreservation on sperm morphology, motility and mitochondrial function. Hum. Reprod. 17:704-709. https://doi.org/10.1093/humrep/17.3.704
Paul, R. K., K. Balaganur, S. V. Bahire, D. Kumar, & R. Singh. 2018. Supplementation of cauda epididymal plasma improves sperm characteristics following liquid preservation of ram semen at 3–5°C. Reprod. Fertil. Dev. 30:1389-1401. https://doi.org/10.1071/RD18063
Praharani, L., S. Supriyati, & R. Krisnan. 2016. A preliminary study on some reproductive traits and heterosis effects of Anglo Nubian and Etawah grade crossbred does. Indonesian Center for Animal Research and Development (ICARD), Indonesia. pp. 252-260. https://doi.org/10.14334/Proc.Intsem.LPVT-2016-p.252-260
Prastiya, R. A., A. Samik, M. T. E. Purnama, & A. L. Saputro. 2019. Protective effect of mycotoxin binders on ovarian gestation mice exposed by zearalenone. Indian Vet. J. 96:44-46.
Ratnani, H., M. N. Ihsan, G. Ciptadi, & S. Suyadi. 2017. Effect of alpha- tocopherol supplementation in the extender on the sperm quality of Maduran bull before and after quick freezing. Int. J. Adv. Res. 5:1378-1389. https://doi.org/10.21474/IJAR01/4849
Sharafi, M., M. Zhandi, A. Shahverdi, & M. Shakeri. 2015. Beneficial effects of nitric oxide induced mild oxidative stress on post-thawed bull semen quality. Int. J. Fertil. Steril. 29:230-237. https://doi.org/10.22074/ijfs.2015.4244
Sikka, S. 2004. Role of oxidative stress and antioxidants in andrology and assisted reproductive technology. J. Androl. 25:5-18. https://doi.org/10.1002/j.1939-4640.2004.tb02751.x
Soares, A. T., S. V. Silva, A. M. Batista, F. C. Almeida, J. F. Nunes, C. A. Peixoto, & M. M. P. Guerra. 2015. Ultrastructure evaluation of goat sperm after freezing in a skim milkbased extender with Trolox supplementation. Andrologia 47:470-476. https://doi.org/10.1111/and.12279
Spirlandeli, A. L., R. Deminice, & A. A. Jordao. 2014. Plasma malondialdehyde as biomarker of lipid peroxidation: effects of acute exercise. Int. J. Sports Med. 35:14–18. https://doi.org/10.1055/s-0033-1345132
Storey, B. T. 2008. Mammalian sperm metabolism: Oxygen and sugar, friend and foe. Int. J. Dev. Biol. 52:427-437. https://doi.org/10.1387/ijdb.072522bs
Tafif, K. 2019. The effect of alpha-tocopherol supplementation in skim milk extender of sapudi ramon on sperm motility, viability, plasma membrane integrity, and cromatin damage. Dissertation, Airlangga University.
Ullah, Z., H. Khan, S. M. Hussain, M. T. Tunio, S. M. R. Dilshad, A. Gohar, H. Zahid, & A. Ali. 2019. Enhancement of extender excellence of frozen bull semen using α-Tocopherol as an antioxidant. Acta Sci. Vet. 47:1641. https://doi.org/10.22456/1679-9216.90447
Wang, W., J. Luo, S.Sun, L. Xi, Q. Gao, A.B. Haile, H. Shi, W. Zhang, & H. Shi. 2015. The effect of season on sperm motility, plasma membrane and acrosome integrity in fresh and frozen-thawed Semen from Xinong Saanen Bucks. Reprod. Domest. Anim. 250:23-28. https://doi.org/10.1111/rda.12444
Widjaya, N. 2017. Pengaruh pemberian susu skim dengan pengencer tris kuning telur terhadap daya tahan hidup sperm sapi pada suhu penyimpanan 5°C (Indonesian). Sains Peternakan: Jurnal Penelitian Ilmu Peternakan. 9:72-76. https://doi.org/10.20961/sainspet.9.2.72-76
Authors
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors submitting manuscripts should understand and agree that copyright of manuscripts of the article shall be assigned/transferred to Tropical Animal Science Journal. The statement to release the copyright to Tropical Animal Science Journal is stated in Form A. This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA) where Authors and Readers can copy and redistribute the material in any medium or format, as well as remix, transform, and build upon the material for any purpose, but they must give appropriate credit (cite to the article or content), provide a link to the license, and indicate if changes were made. If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.