Intestinal Morphology, Energy Availability, and Growth Performance of Broilers Treated with the Combination of Probiotic and Inulin

H. Julendra, A. Sofyan, L. Istiqomah, M. F. Karimy, Abinawanto Abinawanto, Yasman Yasman


Probiotic and prebiotic or their combinations can potentially function as an alternative to antibiotics growth promoters (AGPs) for broiler. This study was designed to investigate the growth performance, intestinal microstructure, and nutrients digestibility of broilers administered with probiotics of Lactobacillus plantarum AKK30 and Saccharomyces cerevisiae B18 in combination with inulin. A total of 275 male chickens (initial bodyweight of 47±0.05 g) were reared for growth performance evaluation. At the 32-d-old, 25 male chickens were necropsied for intestinal microstructural analysis, while the other 25 male chickens were selected for evaluation of digestibility (body weight= 1525±0.08 g). Treatments of probiotics in combination with different levels of inulin consisted of control with probiotics without inulin (S0), probiotics with 0.5% of inulin (S1), probiotics with 1.0% of inulin (S2), probiotics with 1.5% of inulin (S3), and commercial probiotics without inulin (Sc), which were arranged in a completely randomized design with five replications. Results showed that body weight gain and performance index in broilers treated prebiotics in combination with 0.5% inulin (S1), 1% inulin (S2), and commercial probiotic without inulin (Sc) were significantly higher (p<0.05) than those in control broiler chickens that were treated with probiotics without inulin. Feed intake showed no differences among treatments, whereas feed conversion ratios in broiler chickens treated with the commercial probiotics (Sc) or probiotics in combination with inulin at 0.5% (S1) and 1.0% (S2) were lower than control chickens. Metabolizable energy, nitrogen retention, and villi height in chickens treated with probiotics in combination with 0.5% inulin (S1) and 1.0% inulin (S2) were higher than those in the control group. In conclusion, the administration of probiotic combined with inulin at the level of either 0.5 or 1.0% improves broiler performance, intestinal microstructure, and nutrients digestibility.


Anwar, M. I., F. M. Muhammad, M. Awais, & M. Akhtar. 2017. A review of β-glucan as a growth promoter and antibiotic alternative againts enteric pathogens in poultry. World Poult. Sci. J. 73: 651-661.
AOAC. 2005. Official Methods of Analysis of AOAC International 18th ed. Assoc. Off. Anal. Chem., Arlington.
Cohort. 2008.Costat-Coplot Version 6.311. Cohort Software798 Light House Ave. PMB320. Monterey. CA93940 and USA.
deSouza Oliveira, R. P., P. Perego, M. N. deOliveira, & A. Converti. 2012. Effect of inulin on the growth and metabolism of a probiotic strain of Lactobacillus rhamnosus in co-culture with Streptococcus thermophilus. LWT Food Sci. Technol. 47: 358-363.
Gadde, U., W. H. Kim, S. T. Oh, & H. S. Lillehoj. 2017. Alternatives to antibiotics for maximizing growth performance and feed efficiency in poultry: a review. Anim. Health Res. Rev. 18: 26-45.
Gao, Z., H. Wu, L. Shi, X. Zhang, R. Sheng, F. Yin, & R. Gooneratne. 2017. Study of Bacillus subtilis on growth performance, nutrition metabolism and intestinal microflora of 1 to 42 d broiler chickens. Anim. Nutr. 3: 109-113.
Ghasemi, H., M. Shivazad, K. Esmaeilnia, H. Kohram, & M.A. Karimi. 2010. The effect of synbiotic containing Enterococcus faecium and inulin on growth performance and resistance to coccidiosis in broiler chickens. J. Poult. Sci. 47: 149-155.
Hoffman, J. D., L.M. Yanckello, G. Chlipala, T.C. Hammond, S.D. McCulloch, I. Parikh, S. Sun, S.J. Green, & A.L. Lin. 2019. Dietary inulin alters the gut microbiome, enhances systemic metabolism and reduces neuroinflammation in an APOE4 mouse model. PloS One 14: e0221828.
Huang, Q., Y. Wei, Y. Lv, Y. Wang, & T. Hu. 2015. Effect of dietary inulin supplements on growth performance and intestinal immunological parameters of broiler chickens. Livest. Sci. 180: 172-176.
Iriyanti, N., B. Hartoyo, & S. Suhermiyati. 2018. Performance and intestinal profiles of Tegal duck fed ration supplemented with prebiotics. Trop. Anim. Sci. J. 41: 15-21.
Istiqomah, L., S. N. Hayati, E. Damayanti, H. Julendra, A. A. Sakti, & T. Untari. 2013. Performance and meat quality of broilers infected with Escherichia coli and administered with bio additive, probiotic, and antibiotic. Med. Pet. 36: 14-20.
JCI (Japfa Comfeed Indonesia). 2020. Brochure MB202 and MB402. PT. Multi Breeder Japfa Comfeed Indonesia. [17 April 2020].
Jiang, S., A. A. Mohammed, J. A. Jacobs, T.A. Cramer, & H. W. Cheng. 2020. Effect of synbiotic on thyroid hormones, intestinal histomorphology, and heat shock protein 70 expression in broiler chickens reared under cyclic heat stress. Poult. Sci. 99: 142-150.
Kalia, S., V. K. Bharti, D. Gogoi, A. Giri, & B. Kumar. 2017. Studies on the growth performance of different broiler strains at high altitude and evaluation of probiotic effect on their survivability. Sci. Rep. 11: 46074.
Kavoi, B. M., D.W. Gakuya, P.N. Mbugua, & S.G. Kiama. 2016. Effects of dietary Moringa oleifera leaf meal supplementation on chicken intestinal structure and growth performance. J. Morphol. Sci. 33:186-192.
Kowalczuk-Vasilev, E., E. R. Grela, W. Samolinska, R. Klebaniuk, B. O. Ż. E. N. A. Kiczorowska, R. Krusinski, A. Winiarska-Mieczan, K. Kepka, & M. Kwiecien. 2017. Blood metabolic profile of broiler chickens fed diets with different types and levels of inulin. Med. Wet. 73: 774-780. htpps://
Kridtayopas, C., C. Rakangtong, C. Bunchasak, & W. Loongyai. 2019. Effect of prebiotic and synbiotic supplementation in diet on growth performance, small intestinal morphology, stress, and bacterial population under high stocking density condition of broiler chickens. Poult. Sci. 98: 4595-4605. htpps://
Leeson, S. & J. D. Summers. 2005. Commercial Poultry Nutrition. Nottingham University Books, Guelph, Ontario, Canada.
McGlone, J. 2010. Guide for The Care and Use of Agricultural Animals in Research and Teaching. Fed. Anim. Sci. Soc. 3rd ed. [5 May 2019].
Mehdi, Y., M. P. Létourneau-Montminy, M. L. Gaucher, Y. Chorfi, G. Suresh, T. Rouissi, S. K. Brar, C. Côté, A. A. Ramirez, & S. Godbout. 2018. Use of antibiotics in broiler production: global impacts and alternatives. Anim. Nut. 4: 170-178.
Mishra, B. & R. Jha. 2019. Oxidative stress in the poultry gut: potential challenges and interventions. Front. Vet. Sci. 6: 60.
Mohammed, A. A., S. Jiang, J. A. Jacobs, & H. W. Cheng. 2019. Effect of a synbiotic supplement on cecal microbial ecology, antioxidant status, and immune response of broiler chickens reared under heat stress. Poult. Sci. 98: 4408-4415.
Mookiah, S., C. C. Sieo, K. Ramasamy, N. Abdullah, & Y. W. Ho. 2014. Effects of dietary prebiotics, probiotic and synbiotic on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens. J. Sci. Food Agric. 94: 341-348.
NRC (National Research Council). 1994. Nutrient Requirements of Poultry. 9th Rev. ed. National Academy Press, Washington DC
Pranckute, R., K. Arnoldas, K. Nomeda, & J. C. Donaldas. 2016. Combining prebiotics with probiotic bacteria can enhance bacterial growth and secretion of bacteriocins. Int. J. Bio. Macromol. 89: 669-676.
Ricke, S. C., S. I. Lee, S. A. Kim, S. H. Park, & Z. Shi. 2020. Prebiotics and the poultry gastrointestinal tract microbiome. Poult. Sci. 99: 670-677.
Ripon, M. M. R., M. H. Rashid, M. M. Rahman, M. F. Ferdous, M. S. Arefin, A. A. Sani, M. T. Hossain, M. U. Ahammad, & K. Rafiq. 2019. Dose-dependent response to phytobiotic supplementation in feed on growth, hematology, intestinal pH, and gut bacterial load in broiler chicken. J. Adv. Vet. Anim. Res. 6: 253-259. htpps://
Salah, A. S., M. S. El-Tarabany, & M. A. Ali. 2019. Impact of dietary supplementation with a synbiotic, organic acids or their combination on growth performance, carcass traits, economic efficiency, jejunum histomorphometry and some blood indices of broiler chickens. Anim. Prod. Sci. 59: 1318-1326.
Sarangi, N. R., L. K. Babu, A. Kumar, C. R. Pradhan, P. K. Pati, & J. P. Mishra. 2016. Effect of dietary supplementation of prebiotic, probiotic, and synbiotic on growth performance and carcass characteristics of broiler chickens. Vet. World 9: 313-319.
Sibbald, I. R. & M. S. Wolynetz. 1985. Relationships between estimates of bioavailable energy made with adult cockerels and chicks: effects of feed intake and nitrogen retention. Poult. Sci. 64: 127-138.
Śliżewska, K., P. Markowiak-Kopeć, A. Żbikowski, & P. Szeleszczuk. 2020. The effect of synbiotic preparations on the intestinal microbiota and her metabolism in broiler chickens. Sci. Rep. 10: 1-13.
Sofyan, A., M. Angwar, H. Herdian, E. Damayanti, L. Istiqomah, A. Febrisiantosa, H. Julendra, M. H. Wibowo, & T. Untari. 2012. Performance enhancement and immunity profile of broiler treated feed additive containing lactic acid bacteria and Ganoderma lucidum. Med. Pet. 35: 201-206.
Sofyan, A., R. S. H. Martin, E. B. Laconi, A. Jayanegara, H. Julendra, E. Damayanti, & A. E. Suryani. 2019. The assays of bacteria-yeast consortia as probiotics candidates and their influences on nutrients utilization of quails diet. Trop. Anim. Sci. J. 42: 196-202.
Sohail, M. U., M.E. Hume, J. A. Byrd, D.J. Nisbet, A. Ijaz, A. Sohail, M. Z. Shabbir, & H. Rehman. 2012. Effect of supplementation of prebiotic mannan-oligosaccharides and probiotic mixture on growth performance of broilers subjected to chronic heat stress. Poult. Sci. 91: 2235-2240.
Sun, H. Y, & I. H. Kim. 2019. Dietary supplementation of mixed yeast culture derived from Saccharomyces cerevisiae and Kluyveromyces maxianus: Effects on growth performance, nutrient digestibility, meat quality, blood parameters, and gut health in broilers. J. Poult. Sci. 56: 140-147.
Tayeri, V., A. Seidavi, L. Asadpour, & C. J. C. Phillips. 2018. A comparison of the effects of antibiotics, probiotics, synbiotic and prebiotics on the performance and carcass characteristics of broilers. Vet. Res. Commun. 42: 195-207.
Titze, B. & G. Christel. 2016. Volume scanning electron microscopy for imaging biological ultrastructure. Biol. Cell. 108: 307-337. boc.201600024
Villagrán-de la Mora, Z., K. Nuño, O. Vázquez-Paulino, H. Avalos, J. Castro-Rosas, C. Gómez-Aldapa, A. C. Angulo, A. Felipe, & A. Villarruel-López. 2019. Effect of a synbiotic mix on intestinal structural changes, and Salmonella typhimurium and Clostridium perfringens colonization in broiler chickens. Animals 9: 777.
Wang, J., S. Wang, H. Liu, D. Zhang, Y. Wang, & H. Ji. 2019. Effects of oligosaccharides on the growth and stress tolerance of Lactobacillus plantarum ZLP001 in vitro, and the potential synbiotic effects of L. plantarum ZLP001 and fructo-oligosaccharide in post-weaning piglets. J. Anim. Sci. 97: 4588-4597.
Warnes, G.R., B. Bolker, L. Bonebakker, R. Gentleman, W. Huber, A. Liaw, T. Lumley, M. Maechler, A. Magnusson, S. Moeller, M. Schawartz, B. Venables, & T. Galili. 2019. gplots: various R programming tools for plotting data, version [7 April 2019].
Yadav, S, & J. Rajesh. 2019. Strategies to modulate the intestinal microbiota and their effects on nutrient utilization, performance, and health of poultry. J. Anim. Sci. Biotech. 10: 2.
Yang, Y., J. D. Latorre, B. Katri, Y. M. Kown, B. W. Kong, K. D. Teague, L. E. Graham, D. Wolfenden, B. D. Mahaffey, M. Baxter, X. Hernandez-Velasco, R. Moreno-Guzman, B. M. Hargiz, & G. Tellez. 2018. Characterization and evaluation of lactic acid bacteria candidates for intestinal epithelial permeability and Salmonella typhimurium colonization in neonatal turkey poults. Poult. Sci. 97: 515-521.
Yitbarek, A., H. Echeverry, P. Munyaka, & J. C. Rodriguez-Lecompte. 2015. Innate immune response of pullets fed diets supplemented with prebiotics and synbiotic. Poult. Sci. 94: 1802-1811.


H. Julendra (Primary Contact)
A. Sofyan
L. Istiqomah
M. F. Karimy
Abinawanto Abinawanto
Yasman Yasman
Author Biography

H. Julendra, Research Group of Bio-Feed Additive- Research Division for Natural Product Technology (BPTBA)- Indonesian Institute of Sciences (LIPI)

Group Research Bio-Feed Additive

JulendraH., SofyanA., IstiqomahL., KarimyM. F., AbinawantoA., & YasmanY. (2021). Intestinal Morphology, Energy Availability, and Growth Performance of Broilers Treated with the Combination of Probiotic and Inulin. Tropical Animal Science Journal, 44(1), 39-47.

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