Supplementation of Nigella sativa Oil and Honey Prolong the Survival Rate of Fresh and Post-Thawed Goat Sperms
Abstract
Some chemical compositions of polyphenol antioxidants found in Nigella sativa and honey have potential to protect sperm membrane from lipid peroxidation. Therefore, we study the effect of in vitro supplementation of Nigella sativa oil and honey on sperm parameters; motility, progressive score, and abnormalities in fresh and post-thawed goat semen. Pooled semen from five sexually matured males Jermasia goats between aged 2 to 3 years old, with average live weight of 35.9±5.7 kg and average body condition score (BCS) of 1.7±0.45 were used in this study. The semen were added with tris-egg yolk extender and combination of supplements (Nigella sativa oil or/and honey), and divided into groups; Control (no supplementation), Treatment 1 (0.5% v/v Nigella sativa), Treatment 2 (2% v/v honey), and Treatment 3 (0.5% v/v Nigella sativa and 2% v/v honey). All parameters of fresh semen were immediately assessed after semen collection and parameter of post-thawed semen were assessed after 48 hours (2 days) of semen cryopreservation. The fresh sperm parameters were observed at 0, 0.5, 1, 1.5, and 2 hours after semen collection. Results showed that the motility of fresh semen at 1.5 and 2.0 hours after collection treated with Nigella sativa oil (Group 1; 73.8% and 72.0% respectively) and honey (Group 2; 73.3% and 72.0%; respectively) were 20% (1.5 h) and 8% (2.0 h) lower than Controls (93.0% and 79.8% of motility) (P<0.05). The progressive score and sperm abnormality were not significant between groups. While, the motility of post-thawed semen was higher (P<0.05) in treatment 3 (60.33±10.08 of motility; supplemented with Nigella sativa and honey) compare to Controls (24.33±8.17 of motility) at 0 and 0.5 hour after thawing. Thus, we suggested that Nigella sativa oil and honey show a potential as a supplement to goat’s extender with the ability to protect sperm membrane from damage due to oxidative stress and could prevent ice crystal formation during cryopreservation.
References
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Andrabi, S. M. H. & W. M. C. Maxwell. 2007. A review on reproductive biotechnologies for conservation of endangered mammalian species. Anim. Reprod. Sci. 99: 223-243. https://doi.org/10.1016/j.anireprosci.2006.07.002
Ball, B. A. 2008. Oxidative stress, osmotic stress and apoptosis: impacts on sperm function and preservation in the horse. Anim. Reprod. Sci. 107: 257-267. https://doi.org/10.1016/j.anireprosci.2008.04.014
Barbas, J. P., & R. D. Mascarenhas. 2009. Cryopreservation of domestic animal sperm cells. Cell Tissue Bank. 10: 49-62. https://doi.org/10.1007/s10561-008-9081-4
Barbato, V., R. Talevi, S. Braun, A. Merolla, S. Sudhakaran, S. Longobardi, & R. Gualtieri. 2017. Supplementation of sperm media with zinc, D-aspartate and co-enzyme Q10 protects bull sperm against exogenous oxidative stress and improves their ability to support embryo development. Zygote 25: 168-175. https://doi.org/10.1017/S0967199416000459
Bogdanov, S., T. Jurendic, R. Sieber, & P. Gallmann. 2008. Honey for nutrition and health: A Review. J. Am. Coll. Nutr. 27: 677-689. https://doi.org/10.1080/07315724.2008.10719745
Chitra, K. C., C. Latchoumycandane, & P. P. Mathur. 2003. Induction of oxidative stress by bisphenol A in the epididymal sperm of rats. Toxicology. 185: 119-127. https://doi.org/10.1016/S0300-483X(02)00597-8
El-Sheshtawy, R. I., D. A. El-Badry, A. Gamal, W. S. El-Nattat, & A. M. A. Almaaty. 2016. Natural honey as a cryoprotectant to improve Arab stallion post-thawing sperm parameters. Asian Pac. J. Reprod. 5: 31-334. https://doi.org/10.1016/j.apjr.2016.06.004
El-Sheshtawy, R. I., El-Nattat, W. S., Sabra, H. A. & Ali, A. H. 2014. Effect of honey solution on semen preservability of local breeds of cattle bulls. World Appl. Sci. J. 32: 2076-2078.
Eriani, K., A. Azhar, M. Ihdina, B. Rosadi, M. Rizal, & A. Boediono. 2018. Quality enhancement of Aceh swamp buffalo (Bubalus bubalis) frozen semen by supplementing β-carotene. Trop. Anim. Sci. J. 41: 1-7. https://doi.org/10.5398/tasj.2018.41.1.1
Fakhrildin, M. B. M., & R. A. Alsaadi. 2014. Honey supplementation to semen-freezing medium improves human sperm parameters post-thawing. J. Family Reprod. Health. 8: 27-31.
Fuller, B. J. 2004. Cryoprotectants: the essential antifreezes to protect life in the frozen state. Cryo Letters. 25: 375- 388.
Gholamnezhad, Z., S. Havakhah, & M. H. Boskabady. 2016. Preclinical and clinical effects of Nigella sativa and its constituent, thymoquinone: A review. J. Ethnopharmacol. 190: 372-386. https://doi.org/10.1016/j.jep.2016.06.061
Houghton, P. J., Zarka, R., de las Heras, B. & Hoult, J. R. 1995. Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med. 61: 33-36. https://doi.org/10.1055/s-2006-957994
Hu, J. H., W. Q. Tian, X. L. Zhao, L. S. Zan, H. Wang, Q. W. Li, & Y. P. Xin. 2010. The cryoprotective effects of ascorbic acid supplementation on bovine semen quality. Ani. Rep. Sci. 121: 72-77. https://doi.org/10.1016/j.anireprosci.2010.04.180
Jerez, R., Gil, L., Gonzalez, N., Luno, V., Olaciregui, M. & Martinez, F. 2013. Rosemary honey as natural energetic source on refrigeration ram semen. Reprod. Dom. Anim. 48:1-6.
Khader, M. & P. M. Eckl. 2014. Thymoquinone: an emerging natural drug with a wide range of medical applications. Iran J. Basic Med. Sci. 17: 950-957.
Kolahdooz, M., S. Nasri, S. Z. Modarres, S. Kianbakht, & H. F. Huseini. 2014. Effects of Nigella sativa L. seed oil on abnormal semen quality in infertile men: A randomized, double-blind, placebo-controlled clinical trial. Phytomedicine. 21: 901-905. https://doi.org/10.1016/j.phymed.2014.02.006
Kruk, I., T. Michalska, K. Lichszteld, A. Kladna, & H. Y. Aboul-Enein. 2000. The effect of thymol and its derivatives on reactions generating reactive oxygen species. Chemosphere. 41: 1059-1064. https://doi.org/10.1016/S0045-6535(99)00454-3
Mansour, S. W., S. Sibghatullah, H. Sree, A. K. Mueen, & A. R. N. Ibrahim. 2013. Sensibility of male rats fertility against olive oil, Nigella sativa oil and pomegranate extract. Asian. Pac. J. Trop. Biomed. 3: 563-568. https://doi.org/10.1016/S2221-1691(13)60114-8
Olayemi, F. O., D. A. Adenigi, & M. O. Oyeyemi. 2011. Evaluation of sperm motility and viability in honey included egg yolk based extenders. Global Vet. 7: 19-21.
Ozugurlu, F., S. Sahin, N. Idiz, O. Akyol, A. Ilhan, R. Yigitoglu, & B. Isik. 2005. The effect of Nigella sativa oil against experimental allergic encephalomyelitis via nitric oxide and other oxidative stress parameters. Cell Mol Biol (Noisy-le-grand). 51: 337-342.
Pamungkas, F., A. Batubara, & S. Sutoro. 2014. The quality of spermatozoa of Gembrong goats during cryopreservation process. Med. Pet. 37: 95-100. https://doi.org/10.5398/medpet.2014.37.2.95
Parandin, R., N. Yousofvand, & R. Ghorbani. 2012. The enhancing effects of alcoholic extract of Nigella sativa seed on fertility potential, plasma gonadotropins and testosterone in male rats. Int. J. Reprod. Biomed. 10: 355-362.
Perez, E., A. J. Rodriguez-Malaver. 2006. Vit P: Antioxidant capacity of Venezuelan honey in Wistar rat homogenates. J. Med. Food. 9: 510-516. https://doi.org/10.1089/jmf.2006.9.510
Riad, R. M., A. M. Ghoniem, & T. S. T. Seleem. 2004. Effect of Nigella sativa (Aquas extract) on the viability of rabbit spermatozoa, fertility traits and bacterial contamination. Assiut. Vet. Med. J. 130: 899-911.
Salamon, S., & W. M. C. Maxwell. 2000. Storage of ram semen. Anim. Reprod. Sci. 62: 77-111. https://doi.org/10.1016/S0378-4320(00)00155-X
Saleh, R. A., A. Agarwal, E. A. Nada, M. H. El-Tonsy, R. K. Sharma, A. Meyer, & A. J. Thomas. 2003. Negative effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility. Fertil Steril. 79: 1597-1605. https://doi.org/10.1016/S0015-0282(03)00337-6
Salem, M. L. 2005. Immunomodulatory & therapeutic properties of the Nigella sativa L. seed. Int. Immunopharmacol. 5: 1744-1770. https://doi.org/10.1016/j.intimp.2005.06.008
Salmani, H., M. M. Nabi, H. Vaseghi-Dodaran, M. B. Rahman, A. Mohammadi-Sangcheshmeh, M. Shakeri, & M. Zhandi. 2013. Effect of glutathione in soybean lecithin-based semen extender on goat semen quality after freeze-thawing. Small. Rumin. Res. 112: 123-127. https://doi.org/10.1016/j.smallrumres.2012.12.015
Shikh Maidin, M., N. F. Adanan, M. T. Aminudin, & A. Tawang. 2014. In vitro supplements improves motility and progressive score of spermatozoa in Jermasia goats. APCBEE Procedia 8: 329-333. https://doi.org/10.1016/j.apcbee.2014.03.049
Syarifuddin, N. A., A. L. Toleng, D. P. Rahardja, I. Ismartoyo, & M. Yusuf. 2017. Improving libido and sperm quality of Bali bulls by supplementation of Moringa oleifera leaves. Media Peternakan. 40: 88-93. https://doi.org/10.5398/medpet.2017.40.2.88
Al-Sa’aidi, J. A. A., A. L. D. Al-Khuzai, & N. F. H. Al-Zobaydi. 2009. Effect of alcoholic extract of Nigella sativa on fertility in male rats. Iraqi J. Vet. Sci. 23.
Andrabi, S. M. H. & W. M. C. Maxwell. 2007. A review on reproductive biotechnologies for conservation of endangered mammalian species. Anim. Reprod. Sci. 99: 223-243. https://doi.org/10.1016/j.anireprosci.2006.07.002
Ball, B. A. 2008. Oxidative stress, osmotic stress and apoptosis: impacts on sperm function and preservation in the horse. Anim. Reprod. Sci. 107: 257-267. https://doi.org/10.1016/j.anireprosci.2008.04.014
Barbas, J. P., & R. D. Mascarenhas. 2009. Cryopreservation of domestic animal sperm cells. Cell Tissue Bank. 10: 49-62. https://doi.org/10.1007/s10561-008-9081-4
Barbato, V., R. Talevi, S. Braun, A. Merolla, S. Sudhakaran, S. Longobardi, & R. Gualtieri. 2017. Supplementation of sperm media with zinc, D-aspartate and co-enzyme Q10 protects bull sperm against exogenous oxidative stress and improves their ability to support embryo development. Zygote 25: 168-175. https://doi.org/10.1017/S0967199416000459
Bogdanov, S., T. Jurendic, R. Sieber, & P. Gallmann. 2008. Honey for nutrition and health: A Review. J. Am. Coll. Nutr. 27: 677-689. https://doi.org/10.1080/07315724.2008.10719745
Chitra, K. C., C. Latchoumycandane, & P. P. Mathur. 2003. Induction of oxidative stress by bisphenol A in the epididymal sperm of rats. Toxicology. 185: 119-127. https://doi.org/10.1016/S0300-483X(02)00597-8
El-Sheshtawy, R. I., D. A. El-Badry, A. Gamal, W. S. El-Nattat, & A. M. A. Almaaty. 2016. Natural honey as a cryoprotectant to improve Arab stallion post-thawing sperm parameters. Asian Pac. J. Reprod. 5: 31-334. https://doi.org/10.1016/j.apjr.2016.06.004
El-Sheshtawy, R. I., El-Nattat, W. S., Sabra, H. A. & Ali, A. H. 2014. Effect of honey solution on semen preservability of local breeds of cattle bulls. World Appl. Sci. J. 32: 2076-2078.
Eriani, K., A. Azhar, M. Ihdina, B. Rosadi, M. Rizal, & A. Boediono. 2018. Quality enhancement of Aceh swamp buffalo (Bubalus bubalis) frozen semen by supplementing β-carotene. Trop. Anim. Sci. J. 41: 1-7. https://doi.org/10.5398/tasj.2018.41.1.1
Fakhrildin, M. B. M., & R. A. Alsaadi. 2014. Honey supplementation to semen-freezing medium improves human sperm parameters post-thawing. J. Family Reprod. Health. 8: 27-31.
Fuller, B. J. 2004. Cryoprotectants: the essential antifreezes to protect life in the frozen state. Cryo Letters. 25: 375- 388.
Gholamnezhad, Z., S. Havakhah, & M. H. Boskabady. 2016. Preclinical and clinical effects of Nigella sativa and its constituent, thymoquinone: A review. J. Ethnopharmacol. 190: 372-386. https://doi.org/10.1016/j.jep.2016.06.061
Houghton, P. J., Zarka, R., de las Heras, B. & Hoult, J. R. 1995. Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med. 61: 33-36. https://doi.org/10.1055/s-2006-957994
Hu, J. H., W. Q. Tian, X. L. Zhao, L. S. Zan, H. Wang, Q. W. Li, & Y. P. Xin. 2010. The cryoprotective effects of ascorbic acid supplementation on bovine semen quality. Ani. Rep. Sci. 121: 72-77. https://doi.org/10.1016/j.anireprosci.2010.04.180
Jerez, R., Gil, L., Gonzalez, N., Luno, V., Olaciregui, M. & Martinez, F. 2013. Rosemary honey as natural energetic source on refrigeration ram semen. Reprod. Dom. Anim. 48:1-6.
Khader, M. & P. M. Eckl. 2014. Thymoquinone: an emerging natural drug with a wide range of medical applications. Iran J. Basic Med. Sci. 17: 950-957.
Kolahdooz, M., S. Nasri, S. Z. Modarres, S. Kianbakht, & H. F. Huseini. 2014. Effects of Nigella sativa L. seed oil on abnormal semen quality in infertile men: A randomized, double-blind, placebo-controlled clinical trial. Phytomedicine. 21: 901-905. https://doi.org/10.1016/j.phymed.2014.02.006
Kruk, I., T. Michalska, K. Lichszteld, A. Kladna, & H. Y. Aboul-Enein. 2000. The effect of thymol and its derivatives on reactions generating reactive oxygen species. Chemosphere. 41: 1059-1064. https://doi.org/10.1016/S0045-6535(99)00454-3
Mansour, S. W., S. Sibghatullah, H. Sree, A. K. Mueen, & A. R. N. Ibrahim. 2013. Sensibility of male rats fertility against olive oil, Nigella sativa oil and pomegranate extract. Asian. Pac. J. Trop. Biomed. 3: 563-568. https://doi.org/10.1016/S2221-1691(13)60114-8
Olayemi, F. O., D. A. Adenigi, & M. O. Oyeyemi. 2011. Evaluation of sperm motility and viability in honey included egg yolk based extenders. Global Vet. 7: 19-21.
Ozugurlu, F., S. Sahin, N. Idiz, O. Akyol, A. Ilhan, R. Yigitoglu, & B. Isik. 2005. The effect of Nigella sativa oil against experimental allergic encephalomyelitis via nitric oxide and other oxidative stress parameters. Cell Mol Biol (Noisy-le-grand). 51: 337-342.
Pamungkas, F., A. Batubara, & S. Sutoro. 2014. The quality of spermatozoa of Gembrong goats during cryopreservation process. Med. Pet. 37: 95-100. https://doi.org/10.5398/medpet.2014.37.2.95
Parandin, R., N. Yousofvand, & R. Ghorbani. 2012. The enhancing effects of alcoholic extract of Nigella sativa seed on fertility potential, plasma gonadotropins and testosterone in male rats. Int. J. Reprod. Biomed. 10: 355-362.
Perez, E., A. J. Rodriguez-Malaver. 2006. Vit P: Antioxidant capacity of Venezuelan honey in Wistar rat homogenates. J. Med. Food. 9: 510-516. https://doi.org/10.1089/jmf.2006.9.510
Riad, R. M., A. M. Ghoniem, & T. S. T. Seleem. 2004. Effect of Nigella sativa (Aquas extract) on the viability of rabbit spermatozoa, fertility traits and bacterial contamination. Assiut. Vet. Med. J. 130: 899-911.
Salamon, S., & W. M. C. Maxwell. 2000. Storage of ram semen. Anim. Reprod. Sci. 62: 77-111. https://doi.org/10.1016/S0378-4320(00)00155-X
Saleh, R. A., A. Agarwal, E. A. Nada, M. H. El-Tonsy, R. K. Sharma, A. Meyer, & A. J. Thomas. 2003. Negative effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility. Fertil Steril. 79: 1597-1605. https://doi.org/10.1016/S0015-0282(03)00337-6
Salem, M. L. 2005. Immunomodulatory & therapeutic properties of the Nigella sativa L. seed. Int. Immunopharmacol. 5: 1744-1770. https://doi.org/10.1016/j.intimp.2005.06.008
Salmani, H., M. M. Nabi, H. Vaseghi-Dodaran, M. B. Rahman, A. Mohammadi-Sangcheshmeh, M. Shakeri, & M. Zhandi. 2013. Effect of glutathione in soybean lecithin-based semen extender on goat semen quality after freeze-thawing. Small. Rumin. Res. 112: 123-127. https://doi.org/10.1016/j.smallrumres.2012.12.015
Shikh Maidin, M., N. F. Adanan, M. T. Aminudin, & A. Tawang. 2014. In vitro supplements improves motility and progressive score of spermatozoa in Jermasia goats. APCBEE Procedia 8: 329-333. https://doi.org/10.1016/j.apcbee.2014.03.049
Syarifuddin, N. A., A. L. Toleng, D. P. Rahardja, I. Ismartoyo, & M. Yusuf. 2017. Improving libido and sperm quality of Bali bulls by supplementation of Moringa oleifera leaves. Media Peternakan. 40: 88-93. https://doi.org/10.5398/medpet.2017.40.2.88
Authors
Shikh MaidinM., PadlanM. H., AzuanS. A. N., JonitR., MohammedN. H., & AbdullahR. (2018). Supplementation of Nigella sativa Oil and Honey Prolong the Survival Rate of Fresh and Post-Thawed Goat Sperms. Tropical Animal Science Journal, 41(2), 94-99. https://doi.org/10.5398/tasj.2018.41.2.94
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