Anti-diabetic Potency and Characteristics of Probiotic Goat-Milk Yogurt Supplemented with Roselle Extract during Cold Storage
This study aimed to evaluate the anti-diabetic potency by in vitro method and the characteristics of probiotic goat-milk yogurt supplemented with roselle extract during cold storage. The experiment used three treatments namely yogurt, probiotic yogurt, and probiotic yogurt supplemented with roselle extract. Yogurt was stored in cold storage (4 oC) and was evaluated for the characteristics and anti-diabetic potency on days 0, 5, 10, and 15. The anti-diabetic potency was analyzed by a-glucosidase inhibitory activity. The results showed that goat milk in this study had a good quality including fat, SNF, protein, lactose content, density, and freezing point. Roselle extract had anti-diabetic potency with 87.72% inhibition at 25% concentration and also had 13.64% IC50 value. The anti-diabetic potency was affected by the type of yogurt, storage duration, and their interaction (P<0.05). Viscosity and water activity were affected by storage duration (P<0.05), meanwhile titratable acidity and pH value were affected by the type of yogurt. Total lactic acid bacteria were affected by storage duration, and there was an interaction between the type of yogurt and storage duration. In conclusion, probiotic yogurt supplemented with roselle extract had the highest anti-diabetic potency among all treatments with 36.70% inhibition. The inhibitory activity of probiotic yogurt supplemented with roselle extract decreased during 15 days of cold storage. The inhibition was comparable with acarbose at 0.1-0.5 ppm concentration. Physical, chemical, and microbiological characteristics of yogurt in this study were good and could be consumed up to 15 days of cold storage.
Abdel-Moemin, A.R. 2016. Effect of roselle calyces extact on the chemical and sensory properties of functional cupcakes. Food Sci. Hum. Wellness. 5:230-237. https://doi.org/10.1016/j.fshw.2016.07.003
Abou-Arab, A.A., F.M. Abu-Salem, & E.A. Abou-Arab. 2011. Physico-chemical properties of natural pigments (anthocyanin) extracted from Roselle calyces (Hibiscus sabdariffa). J. Am. Sci. 7:445-456.
Ademiluyi, A.O. & G. Oboh. 2013. Aqueous extracts of roselle (Hibiscus sabdariffa Linn.) varieties inhibit a-amylase and a-glucosidase activities in vitro. J. Med. Food. 16: 88–93. https://doi.org/10.1089/jmf.2012.0004
Adisakwattana, S., T. Ruengsamran, P. Kampa, & W. Sompong. 2012. In vitro inhibitory effect of plant-based foods and their combinations on intestinal α-glucosidase and pancreatic α-amylase. BMC Complement. Altern. Med. 12:110-118. https://doi.org/10.1186/1472-6882-12-110
Al-mamun, M., H. Khatun, M.L. Nesa, M.R. Islam, & M.S. Munira. 2015. In vitro evaluation of the antibacterial, cytotoxic and insecticidal acivities of Hibiscus sabdariffa fruits. LARCJI. 2:144-149.
Al-Mandawi, H. 2015. Organic acid composition of different parts of the medical plant – roselle (Hibiscus Sabdariffa). International Journal of Biological & Pharmaceutical Research. 6: 808-813.
AOAC. 2005. Official Methods of Analysis. Association of Official Analytical Chemist. USA.
Apostolidis, E., Y. I. Kwon, & K. Shetty. 2006. Potential of select yogurts for diabetes and hypertension management. J. Food Biochem. 30: 699-717. https://doi.org/10.1111/j.1745-4514.2006.00091.x
Arief, I.I., B.S.L. Jenie, M. Astawan, & A.B. Witarto. 2010. The effectivities of probiotic Lactobacillus plantarum 2C12 and Lactobacillus acidophilus 2B4 as antidiarrheal on rats. Med. Pet. 33:137-143. http://dx.doi.org/10.5398/medpet.2010.33.3.137
Arief, I.I., B.S.L Jenie, M. Astawan, K. Fujiyama, & A.B. Witarto. 2015. Identification and probiotic characteristics of lactic acid bacteria isolated from Indonesian local beef. Asian J. Anim. Sci. 9: 25-36. https://doi.org/10.3923/ajas.2015.25.36
Arief, I.I., C. Budiman, R. Hanifah, M.S. Soenarno. 2016. Antihypertensive potency of goat milk yoghurt supplemented by probiotic and roselle extract. Int. J. Sci.: Basic Appl. Res. 30: 207-214.
Arora, R., N. Bhojak, & R. Joshi. 2013. Comparative aspects of goat and cow milk. Int. J. Eng. Sci. Invention Res. Dev. 2:7-10.
Arungarinathan, G., G.A. McKay, & M. Fisher. 2011. Drugs for diabetes: part 4 acarbose. Br. J. Cardiol. 18:78–81.
Arueya, G.L., & B.O. Akomolafe. 2014. Stability studies of microencapsulated anthocyanins of roselle (Hibiscus sabdariffa L.) in native starch and its potential application in jam production. IOSR J. Environ. Sci. Toxicol. Food Technol. 8:112-122. https://doi.org/10.9790/2402-0871112122
Asgar, M.A. 2013. Anti-Diabetic potential of phenolic compounds: a review. Int. J. Food Prop. 16:91-103. https://doi.org/10.1080/10942912.2011.595864
Astawan, M., T. Wresdiyati, Suliantari, I.I. Arief, & R. Septiawan. 2012. Production of synbiotic yogurt-like using indigenous lactic acid bacteria as functional food. Med. Pet. 35: 9-14. https://doi.org/10.5398/medpet.2012.35.1.9
[BAM] Bacteriological Analytical Manual online. 2001. U.S. Food & Drug Administration. Center for Food Safety & Applied Nutrition.
[BSNI] Badan Standardisasi Nasional Indonesia. 2009. SNI. 01-2981-2009: Yogurt. Jakarta (ID). Badan Standardisasi Nasional.
[BSNI] Badan Standardisasi Nasional Indonesia. 2011. SNI. 01-3141-2011: Susu Segar. Jakarta (ID). Badan Standardisasi Nasional.
Chumsri, P., A. Sirichote, & A. Itharat. 2008. Studies on the optimum conditions for the extraction and concentration of roselle (Hibiscus sabdariffa Linn.) extract. Songklanakarin J. Sci. Technol. 30:133-139.
Cid-Ortega, S. & J.A. Guerrero-Beltran. 2014. Roselle calyces particle size effect on the physicochemical and phytochemicals characteristics. J. Food Res. 3: 83-94. https://doi.org/10.5539/jfr.v3n5p83
CODEX Alimentarius Commission. 2003. CODEX standard for fermented milks. Alimentarius Commission. No. CODEX Stan 243-2003.
Cyrilla, L., B.P. Purwanto, A. Atabany, D.A. Astuti, & A. Sukmawati. 2015. Improving milk quality for dairy goat farm development. Med. Pet. 38:204-211. https://doi.org/10.5398/medpet.2015.38.3.204
Donkor, O.N., A. Henriksson, T. Vasiljevic, & N.P. Shah. 2006. Effect of acidification on the activity of probiotics in yoghurt during cold storage. Int. Dairy J. 16: 1181-1189. https://doi.org/10.1016/j.idairyj.2005.10.008
El-Baily, A.R. 2016. Chemical, microbiological and sensory evaluation of probiotics beverages prepared with permeate and roselle. Int. J. Curr. Microbiol. App. Sci. 5:802-811. https://doi.org/10.20546/ijcmas.2016.501.082
Getaneh, G., A. Mebrat, A. Wubie, & H. Kendie. 2016. Review on goat milk composition and its nutritive value. J. Nutrs. Health Sci. 3:401-409.
Gondokesumo, M.E., H.S.W. Kusuma, & W. Widowati. 2017. α-/β-Glucosidase and α-amylase inhibitory activities of roselle (Hibiscus sabdariffa L.) ethanol extract. Mol. Cell Biomed. Sci. 1:34-40. https://doi.org/10.21705/mcbs.v1i1.3
Guetouache, M., G. Bettache, & M. Samir. 2014. Composition and nutritional value of raw milk. Issues Biol. Sci. Pharm. Res. 2: 115-122.
Hanifah, R., I.I. Arief, C. Budiman. 2016. Antimicrobial activity of goat milk yoghurt with addition of a probiotic Lactobacillus acidophillus IIA-2B4 and roselle (Hibiscus sabdariffa L) extract. Int. Food Res. J. 23: 2638-2645.
Hariyadi, P. 2013. Freeze Drying: for Better Quality & Flavor of Dried Products. Foodreview Indonesia. 3:52-57.
Jaafar, S.H.S., R. Hashim, Z. Hassan, & N. Arifin. 2018. Comparative study on physicochemical characteristics of raw goat milk collected from different farms in Malaysia. Trop. Life Sci. Res. 29:195-212. https://doi.org/10.21315/tlsr2018.29.1.13
Jitendra, K. & P. Amit. 2015. An overview of prospective study on functional food. Int. J. Recent Sci. Res. 6:5497-5500.
Kilima, M., S.F. Remberg, B.E. Chove, & T. Wicklund. 2014. Physio-chemical, mineral composition and antioxidant properties of roselle (Hibiscus sabdariffa L.) extract blended with tropical fruit juices. Afr. J. Food Agric. Nutr. Dev. 14:8963-8978.
Lacroix, I.M. & E.C.Y. Li-Chan. 2013. Inhibition of dipeptidyl peptidase (DPP)-IV and α-glucosidase activities by pepsintreated whey proteins. J. Agric. Food Chem. 61:7500–7506. https://doi.org/10.1021/jf401000s
Lawin, P. & T. Kongbangkerd. 2010. Development of probiotic yoghurt mixed with roselle syrup. KKU Res. J. 15:803-808.
Malelak, G. E. M., H. J. D. Lalel, P. R. Kale, & I. G. N. Jelantik. 2017. The sensory properties, color, microbial, lipid oxidation, and residual nitrite of se’i marinated with lime and roselle calyces extracts. Med. Pet. 40:194-201. https://doi.org/10.5398/medpet.2017.40.3.194
Marwati, T., N. Cahyaningrum, S. Widodo, T. Januarsyah, & Purwoko. 2018. Inhibitory activity of bacteriocin produced from Lactobacillus SGC 1223 toward L.monocytogenes, S. thypimurium and E. coli. IOP Conf Ser: Earth Environ Sci.102.
Ministry of Agriculture and Cooperatives. 2008. Thai Agricultural Standard (TAS) 606-2008 Raw Goat Milk. The Royal Thai Government Gazette, 139(139D). Thailand.
Nakhaee, A. & M. Sanjari. 2013. Evaluation of effect of acarbose consumption on weight losing in non-diabetic overweight or obese patients in kerman. J Res. Med. Sci. 18: 391-394.
Nudyanto, A. & E. Zubaidah. 2014. Isolasi bakteri asam laktat penghasil eksopolisakarida dari kimchi. Jurnal Pangan dan Agroindustri. 3:743-748.
Papadimitriou, K., A. Alegria, P.A. Bron, M. de Angelis, M. Gobbetti, M. Kleerebezem, J.A. Lemos, D.M. Linares, P. Ross, C. Stanton, F. Turroni, D.V. Sinderen, P. Varmanen, M. Ventura, M. Zuniga, E. Tsakalidou, & J. Kok. 2016. Stress physiology of lactic acid bacteria. Microbiol. Mol. Biol. Rev. 80:837-890. https://doi.org/10.1128/MMBR.00076-15
Patil, P., S. Mandal, S.K. Tomar, & S. Anand. 2015. Food protein-derived bioactive peptides in management of type 2 diabetes. Eur. J. Nutr. 54:863-881. https://doi.org/10.1007/s00394-015-0974-2
Purbowati, I.S.M., K. Syamsu, E. Warsiki, & H.S. Rukmini. 2015. Evaluation on toxicity, antibacterial and antioxidant capacity of roselle bioactive compounds with varying solvents. Jurnal Teknologi Industri Pertanian. 25:182-189.
Rosak, C. & G. Mertes. 2012. Critical evaluation of the role of acarbose in the treatment of diabetes: patient considerations. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy. 5:357-367. https://doi.org/10.2147/DMSO.S28340
Sancheti, S., S. Sandesh, & S. Sung-Yum. 2009. Chaenomeles Sinensis: a potent and a- and β-Glucosidase inhibitor. Am. J. Pharmacol. Toxicol. 4: 8-11. https://doi.org/10.3844/ajptsp.2009.8.11
Shori, A.B. & A.S. Baba. 2014. Comparative antioxidant activity, proteolysis and in vitro α-amylase and α-glucosidase inhibition of Allium sativum-yogurts made from cow and camel milk. J. Saudi Chem. Soc. 18: 456-463. https://doi.org/10.1016/j.jscs.2011.09.014
Steel, R.G.D. & J.H. Torrie. 1980. Principles and Procedures of Statistics. McGraw-Hill Book Co. Inc. New York.
Suharto, E.L.S., I.I. Arief, & E. Taufik. 2016. Quality and antioxidant activity of yogurt supplemented with roselle during cold storage. Med. Pet. 39: 82-89. https://doi.org/10.5398/medpet.2016.39.2.82
Sulaiman, N. B., I. I. Arief, & C. Budiman. 2016. Characteristics of lamb sausages fermented by Indonesian meat-derived probiotic, Lactobacillus plantarum IIA-2C12 and Lactobacillus acidophilus IIA-2B4. Med. Pet. 39:104-111. https://doi.org/10.5398/medpet.2016.39.2.104
Sulistijowati, S. 2012. Potensi Lactobacillus acidophilus ATCC 4796 sebagai biopreservatif pada rebusan daging ikan tongkol. Indonesian Journal of Applied Sciences. 2:604-613.
Sulistiyani, M. Safithri, & Y.P. Sari. 2016. Inhibition of α-glucosidase activity by ethanolic extract of Melia azedarach L. leaves. IOP Conf Ser: Earth Environ Sci. 31.
Tidona, F., A. Criscione, A.M. Guastella, A. Zuccaro, S. Bordonaro, & D. Marletta. 2009. Milk-derived bioactive peptides in dairy products. Ital. J. Anim. Sci. 8:315-340. https://doi.org/10.4081/ijas.2009.315
Tomovska, J., N. Gjorgievski, & B. Makarijoski. 2016. Examination of pH, titrable acidity and antioxidant activity in fermented milk. J. Mat. Sci. Eng. 6:326-333.
Tsai, P.J., J. Melntosh, P. Pearce, B. Camden, & B.R. Jordan. 2002. Anthocyanin and antioxidant capacity in roselle (Hibiscus sabdariffa L) extract. Food Res. Int. 35:351- 356. https://doi.org/10.1016/S0963-9969(01)00129-6
[WHO] World Health Organization. 2016. Global Report on Diabetes. WHO Library Cataloguing-in-Publication Data.
Yu, Z., Y. Yin, W. Zhao, Y. Yu, B. Liu, J. Liu, & F. Chen. 2011. Novel peptides derived from egg white protein inhibiting alpha-glucosidase. Food Chem. 129: 1376−1382. https://doi.org/10.1016/j.foodchem.2011.05.067
Zagorska, J. & I. Ciprovica. 2013. Evaluation of factors affecting freezing point of milk. International Scholarly and Scientific Research & Innovation. 7:106-111.
Zeng, Z., J. Luo, F. Zuo, Y. Zhang, H. Ma, & S. Chen. 2016. Screening for potential novel probiotic Lactobacillus strains based on high dipeptidyl peptidase iv and a-glucosidase inhibitory activity. J. Funct. Food. 20: 486-495. https://doi.org/10.1016/j.jff.2015.11.030
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