MEDIA BERINDIKATOR WARNA SEBAGAI PENDETEKSI Salmonella typhimurium

  • Endang Warsiki, Mulyorini Rahayuningsih, dan Roseiga Retno Anggarani

Abstract

Smart label is a label which could inform the quality and provide safety assurance of the food product.
In this research, the label was produced from colored media and purposed to detect the growth of Salmonella.
typhimurium. This bacterium is a pathogenic bacterium that causes salmonellasis disease with symptoms of
poisoning type of infection. This bacterium is commonly appear in the fresh meat and meat product. The rapid
detection of this bacterium would assure the freshness and safety of the meat. This research was aimed to
produce the media based in color changing for rapid detection of the presence of S. typhimurium. The media
indicator was produced from 2% (w/v) agar powder, 0.5% (w/v) tapioca starch, 1% (w/v) glycerol and 1% (w/v)
selective media and then dissolved into distilled water until 100 mL solution. Four kind of selective medias were
used, i.e. Xylose Lysine Deoxychoalate agar (XLD), Hektoen Enteric Agar (HEA), Salmonella Shigela Agar
(SSA) and Bismuth Salt Agar (BSA). XLD media was found to be a very sensitive media to the S. typhimurium
growth and presented a transparent color change to pink color that can be seen visually. The concentration
range of 1-1.5% (w/v) of XLD was the best to develop the media. On the other hand, BSA and SSA media was
notsensitiveon the S. typhimurium. Furthermore, another enrichment media of Brain Heart Infussion (BHI) with
phenol red indicator has resulted in the rapid detection of 24 h after incubation compare to media without BHI
in the same time. This media indicator gave in changing color from red to yellow.
Keywords: media indicator, selective media, color changing, S. typhimurium

Published
2017-04-18