PENGGUNAAN SUBSTRAT WHEY TAHU UNTUK PRODUKSI BIOMASSA OLEH PEDIOCOCCUS PENTOSACEUS E.1222

Abstract

Lactic acid bacteria (LAB) has been widely used as a starter culture. The most important factor for
developing industrial starter culture is the selection of a good and cheap medium. Tofu whey (TW) is the liquid
waste of the tofu production which potentially be able to use as a growth medium for LAB. On this research, TW
was fermented by Pediococcus pentosaceus E.1222 for 24 hour, at 37°C, pH 6, and without agitation. This work
was conducted in several stages, first stage was selection of carbon source and the result showed that TW
supplemented glucose gives the highest LAB number was 4.5×108 CFU/mL and spesific growth rate (μmax) 0.65
h-1. The next stage was determining glucose concentration and the highest growth was TW supplemented 5%
glucose, where the number of LAB reached 1.4×1010 CFU/mL and μmax 0.91 h-1. The next stage was scaling up
the starter production, fermentations were initially performed at scale 150 mL medium, then medium was scaled
up to 500 mL and 1 L. The result showed a slight decreasing in number of LAB on each volume, from 1.1×1010
CFU/mL (150 mL), 1.09×1010 CFU/mL (500 mL), and 1.03×1010 CFU/mL (1L); and declining of μmax is found to
be 0.91–0.90 h-1
.
Keyword: Lactic acid bacteria, starter culture, tofu whey, Pediococcus pentosaceus