Prevalence of bacterial contamination on seafoods products collected from traditional fish market in Bali Province during 2023 Prevalensi kontaminasi bakteri pada produk hasil ikan laut yang dikumpulkan dari pasar ikan tradisional di Provinsi Bali selama tahun 2023
Abstract
Seafood provides essential nutrients beneficial for human health; however, it is highly vulnerable to harmful bacterial infections that pose significant public health risks. This research seeks to assess the prevalence of five categories of seafood obtained from various traditional fish markets in Bali Province. A total of 108 tuna samples, 78 pelagic fish samples, 37 cephalopod samples, 14 sardine samples, and 53 demersal fish samples were collected from various traditional markets in Bali Province. This research evaluated the prevalence of E. coli, coliforms, Salmonella, V. cholerae, and V. parahaemolyticus. The study revealed that the highest prevalence of E. coli, coliform, and V. parahaemolyticus contamination in tuna samples was 95 (87%), 95 (87%), and 103 (95%), respectively. The study indicated that E. coli and coliforms were present in 73 of the 78 pelagic fish samples, representing 93% contamination. Sardine samples exhibited the lowest prevalence of bacteria. All seafood samples, however, tested negative for Salmonella and V. cholera. PCR products from E. coli and V. parahaemolyticus isolates were effectively amplified for the target genes utilized in this study. Local seafood markets should adopt appropriate handling and storage practices to enhance seafood quality. This study emphasizes the significant presence of E. coli, coliforms, and V. parahaemolyticus in seafood, along with the potential health risks posed by specific strains and their antibiotic resistance.
References
Atwill, E. R., & Jeamsripong, S. (2021). Bacterial diversity and potential risk factors associated with Salmonella contamination of seafood products sold in retail markets in Bangkok, Thailand. PeerJ, 9, e12694. https://doi.org/10.7717/peerj.12694
Beal, M. A., Meier, M. J., Dykes, A., Yauk, C. L., Lambert, I. B., & Marchetti, F. (2023). The functional mutational landscape of the lacZ gene. IScience, 26(12), 108407. https://doi.org/10.1016/j.isci.2023.108407
Belleggia, L., & Osimani, A. (2023). Fermented fish and fermented fish-based products, an ever-growing source of microbial diversity: A literature review. Food Research International, 172, 113112. https://doi.org/10.1016/j.foodres.2023.113112
Brugman, S., Ikeda-Ohtsubo, W., Braber, S., Folkerts, G., Pieterse, C. M. J., & Bakker, P. A. H. M. (2018). A comparative review on microbiota manipulation: lessons from fish, plants, livestock, and human research. Frontiers in Nutrition, 5. https://doi.org/10.3389/fnut.2018.00080
Cabello, F. C., Godfrey, H. P., Buschmann, A. H., & Dölz, H. J. (2016). Aquaculture as yet another environmental gateway to the development and globalisation of antimicrobial resistance. The Lancet Infectious Diseases, 16(7), e127–e133. https://doi.org/10.1016/S1473-3099(16)00100-6
Chandimali, N., Park, E. H., Bak, S.-G., Won, Y.-S., Lim, H.-J., & Lee, S.-J. (2024). Not seafood but seafood: A review on cell-based cultured seafood in lieu of conventional seafood. Food Control, 162, 110472. https://doi.org/10.1016/j.foodcont.2024.110472
Chimalapati, S., Lafrance, A. E., Chen, L., & Orth, K. (2020). Vibrio parahaemolyticus : Basic techniques for growth, genetic manipulation, and analysis of virulence factors. Current Protocols in Microbiology, 59(1). https://doi.org/10.1002/cpmc.131
Elbashir, S., Parveen, S., Schwarz, J., Rippen, T., Jahncke, M., & DePaola, A. (2018). Seafood pathogens and information on antimicrobial resistance: A review. Food Microbiology, 70, 85–93. https://doi.org/10.1016/j.fm.2017.09.011
Fehrenbach, G. W., Murphy, E., Pogue, R., Carter, F., Clifford, E., & Major, I. (2024). Comprehensive analysis and assessment of exposure to enteric viruses and bacteria in shellfish. Marine Environmental Research, 196, 106404. https://doi.org/10.1016/j.marenvres.2024.106404
Ferri, G., Lauteri, C., & Vergara, A. (2022). Antibiotic resistance in the finfish aquaculture industry: a review. Antibiotics, 11(11), 1574. https://doi.org/10.3390/antibiotics11111574
Fukase, E., & Martin, W. (2020). Economic growth, convergence, and world food demand and supply. World Development, 132, 104954. https://doi.org/10.1016/j.worlddev.2020.104954
García-Lorenzo, I., Amigo-Dobaño, L., Garza-Gil, M. D., & Varela-Lafuente, M. (2023). Perceptions in small-scale fisheries regarding institutional, economic, technological and environmental factors. Case study in North-Western Spain. Marine Policy, 150, 105530. https://doi.org/10.1016/j.marpol.2023.105530
Ghatak, S., Srinivas, K., Milton, A. A. P., Priya, G. B., Das, S., & Lindahl, J. F. (2023). Limiting the spillover of zoonotic pathogens from traditional food markets in developing countries and a new market design for risk-proofing. Epidemiology and Health, 45, e2023097. https://doi.org/10.4178/epih.e2023097
González, N., Marquès, M., Nadal, M., & Domingo, J. L. (2020). Meat consumption: Which are the current global risks? A review of recent (2010–2020) evidences. Food Research International, 137, 109341. https://doi.org/10.1016/j.foodres.2020.109341
Grema, H. A., Kwaga, J. K. P., Bello, M., & Umaru, O. H. (2020). Understanding fish production and marketing systems in North-western Nigeria and identification of potential food safety risks using value chain framework. Preventive Veterinary Medicine, 181, 105038. https://doi.org/10.1016/j.prevetmed.2020.105038
Hamad, G. M., Abushaala, N. M., Soltan, O. I. A., Abdel-Hameed, S. M., Magdy, R. M. E., Mohamed Hassan Ahmed, E., Elshaer, S. E., Kamar, A. M., Abo Hashem, R. M., Elghazaly, E. M., Amer, A., Alswat, A. S., Aljumayi, H., & Mahmoud, S. F. (2024). Prevalence and antibacterial effect of natural extracts against Vibrio parahaemolyticus and its application on tilapia fillets. LWT, 209, 116812. https://doi.org/10.1016/j.lwt.2024.116812
Huoy, L., Vuth, S., Hoeng, S., Chheang, C., Yi, P., San, C., Chhim, P., Thorn, S., Ouch, B., Put, D., Aong, L., Phan, K., Nasirzadeh, L., Tieng, S., Bongcam-Rudloff, E., Sternberg-Lewerin, S., & Boqvist, S. (2024). Prevalence of Salmonella spp. in meat, seafood, and leafy green vegetables from local markets and vegetable farms in Phnom Penh, Cambodia. Food Microbiology, 124, 104614. https://doi.org/10.1016/j.fm.2024.104614
Ihsan, B. (2021). Identifikasi bakteri patogen (Vibrio spp. dan Salmonella spp.) yang mengontaminasi ikan layang dan bandeng di pasar tradisional. Jurnal Pengolahan Hasil Perikanan Indonesia, 24(1), 89-96. https://doi.org/10.17844/jphpi.v24i1.34198
Jang, J., Hur, H.-G., Sadowsky, M. J., Byappanahalli, M. N., Yan, T., & Ishii, S. (2017). Environmental Escherichia coli : ecology and public health implications-a review. Journal of Applied Microbiology, 123(3), 570–581. https://doi.org/10.1111/jam.13468
Jeamsripong, S., Khant, W., & Chuanchuen, R. (2020). Distribution of phenotypic and genotypic antimicrobial resistance and virulence genes in Vibrio parahaemolyticus isolated from cultivated oysters and estuarine water. FEMS Microbiology Ecology, 96(8). https://doi.org/10.1093/femsec/fiaa081
Kabiraz, M. P., Majumdar, P. R., Mahmud, M. M. C., Bhowmik, S., & Ali, A. (2023). Conventional and advanced detection techniques of foodborne pathogens: A comprehensive review. Heliyon, 9(4), e15482. https://doi.org/10.1016/j.heliyon.2023.e15482
Karanth, S., Feng, S., Patra, D., & Pradhan, A. K. (2023). Linking microbial contamination to food spoilage and food waste: the role of smart packaging, spoilage risk assessments, and date labeling. Frontiers in Microbiology, 14, 1198124. https://doi.org/10.3389/fmicb.2023.1198124
Koutsoumanis, K., Allende, A., Alvarez‐Ordóñez, A., Bolton, D., Bover‐Cid, S., Chemaly, M., De Cesare, A., Herman, L., Hilbert, F., Lindqvist, R., Nauta, M., Nonno, R., Peixe, L., Ru, G., Simmons, M., Skandamis, P., Baker‐Austin, C., Hervio‐Heath, D., Martinez‐Urtaza, J., … Suffredini, E. (2024). Public health aspects of Vibrio spp. related to the consumption of seafood in the EU. EFSA Journal, 22(7), e8883. https://doi.org/10.2903/j.efsa.2024.8896
Lenzi, A., Marvasi, M., & Baldi, A. (2021). Agronomic practices to limit pre- and post-harvest contamination and proliferation of human pathogenic Enterobacteriaceae in vegetable produce. Food Control, 119, 107486. https://doi.org/10.1016/j.foodcont.2020.107486
Letchumanan, V., Chan, K.-G., & Lee, L.-H. (2014). Vibrio parahaemolyticus: a review on the pathogenesis, prevalence, and advance molecular identification techniques. Frontiers in Microbiology, 5. https://doi.org/10.3389/fmicb.2014.00705
Letchumanan, V., Loo, K.-Y., Law, J. W.-F., Wong, S. H., Goh, B.-H., Ab Mutalib, N.-S., & Lee, L.-H. (2019). Vibrio parahaemolyticus: The protagonist of foodborne diseases. Progress In Microbes & Molecular Biology, 2(1). https://doi.org/10.36877/pmmb.a0000029
Li, M., Xu, H., Tian, Y., Zhang, Y., Jiao, X., & Gu, D. (2023). Comparative genomic analysis reveals the potential transmission of Vibrio parahaemolyticus from freshwater food to humans. Food Microbiology, 113, 104277. https://doi.org/10.1016/j.fm.2023.104277
Mahmoud, B. S. M. (2014). The efficacy of grape seed extract, citric acid and lactic acid on the inactivation of Vibrio parahaemolyticus in shucked oysters. Food Control, 41, 13–16. https://doi.org/10.1016/j.foodcont.2013.12.027
Maulu, S., Nawanzi, K., Abdel-Tawwab, M., & Khalil, H. S. (2021). Fish nutritional value as an approach to children’s nutrition. Frontiers in Nutrition, 8. https://doi.org/10.3389/fnut.2021.780844
Moffo, F., Ndebé, M. M. F., Dah, I., Nkolo, E. Nn., Ngom, R. V., Madjeue, F. N., Wade, A., Tiomo, H., Ngwa, V. N., & Mouiche, M. M. M. (2024). Antimicrobial Resistance Profiles of Escherichia coli and Staphylococcus spp. isolated from locally produced fish and imported fish sold in the Centre Region of Cameroon. Journal of Food Protection, 87(12), 100377. https://doi.org/10.1016/j.jfp.2024.100377
Molina, F., López-Acedo, E., Tabla, R., Roa, I., Gómez, A., & Rebollo, J. E. (2015). Improved detection of Escherichia coli and coliform bacteria by multiplex PCR. BMC Biotechnology, 15(1), 48. https://doi.org/10.1186/s12896-015-0168-2
Mumpuni, F.S., & Hasibuan, S. (2018). Prevalensi mikroba pada produk pindang tongkol skala UKM di Pelabuhan Ratu, Sukabumi. Jurnal Pengolahan Hasil Perikanan Indonesia, 21(3), 480-485.
Narayanan, S. V., Joseph, T. C., Peeralil, S., Mothadaka, M. P., & Lalitha, K. V. (2020). Prevalence, virulence characterization, AMR pattern and genetic relatedness of Vibrio parahaemolyticus isolates from retail seafood of Kerala, India. Frontiers in Microbiology, 11. https://doi.org/10.3389/fmicb.2020.00592
Nordhagen, A., Rizwan, A. A. M., Aakre, I., Moxness Reksten, A., Pincus, L. M., Bøkevoll, A., Mamun, A., Haraksingh Thilsted, S., Htut, T., Somasundaram, T., & Kjellevold, M. (2020). Nutrient composition of demersal, pelagic, and mesopelagic fish species sampled off the coast of Bangladesh and their potential contribution to food and nutrition security—The EAF-Nansen Programme. Foods, 9(6), 730. https://doi.org/10.3390/foods9060730
Novoslavskij, A., Terentjeva, M., Eizenberga, I., Valciņa, O., Bartkevičs, V., & Bērziņš, A. (2016). Major foodborne pathogens in fish and fish products: a review. Annals of Microbiology, 66(1), 1–15. https://doi.org/10.1007/s13213-015-1102-5
Nugroho, H. Y. S. H., Indrawati, D. R., Wahyuningrum, N., Adi, R. N., Supangat, A. B., Indrajaya, Y., Putra, P. B., Cahyono, S. A., Nugroho, A. W., Basuki, T. M., Savitri, E., Yuwati, T. W., Narendra, B. H., Sallata, M. K., Allo, M. K., Bisjoe, A. R., Muin, N., Isnan, W., Ansari, F., … Hani, A. (2022). Toward Water, Energy, and Food Security in Rural Indonesia: A Review. Water, 14(10), 1645. https://doi.org/10.3390/w14101645
Nurilmala, M., Fauzi, S., Mayasari, D., & Batubara, I. (2019). Collagen extraction from yellowfin tuna (Thunnus albacares) skin and its antioxidant activity. Jurnal Teknologi, 81(2). https://doi.org/10.11113/jt.v81.11614
Nurjanah, Baharuddin, T. I., & Nurhayati, T. (2021). Ekstraksi kolagen kulit ikan tuna sirip kuning (Thunnus albacares) menggunakan enzim pepsin dan papain. Jurnal Pengolahan Hasil Perikanan Indonesia, 24(2), 174-187.
Oktariani, A. F., Sudaryatma, P. E., Ramona, Y., Wirasuta, I. M. G., Darmayasa, I. B. G., Wiradana, P. A., & Okabayashi, T. (2023). Heavy metals content in fresh tuna and swordfish caught from Hindian and Pacific Oceans: Health risk assessment of dietary exposure. Veterinary World, 858–868. https://doi.org/10.14202/vetworld.2023.858-868
Penca, J., Said, A., Cavallé, M., Pita, C., & Libralato, S. (2021). Sustainable small-scale fisheries markets in the Mediterranean: weaknesses and opportunities. Maritime Studies, 20(2), 141–155. https://doi.org/10.1007/s40152-021-00222-5
Schmidt, C. V., Raza, H., Olsen, K., & Mouritsen, O. G. (2024). Proximate nutritional composition of roe from fish, crustaceans, mussels, echinoderms, and cephalopods. International Journal of Gastronomy and Food Science, 36, 100944. https://doi.org/10.1016/j.ijgfs.2024.100944
Sheng, L., & Wang, L. (2021). The microbial safety of fish and fish products: Recent advances in understanding its significance, contamination sources, and control strategies. Comprehensive Reviews in Food Science and Food Safety, 20(1), 738–786. https://doi.org/10.1111/1541-4337.12671
Siddiky, N. A., Khan, M. S. R., Sarker, M. S., Bhuiyan, M. K. J., Mahmud, A., Rahman, M. T., Ahmed, M. M., & Samad, M. A. (2022). Knowledge, attitude and practice of chicken vendors on food safety and foodborne pathogens at wet markets in Dhaka, Bangladesh. Food Control, 131, 108456. https://doi.org/10.1016/j.foodcont.2021.108456
Siddiqui, S. A., Singh, S., Bahmid, N. A., & Sasidharan, A. (2024). Applying innovative technological interventions in the preservation and packaging of fresh seafood products to minimize spoilage - A systematic review and meta-analysis. Heliyon, 10(8), e29066. https://doi.org/10.1016/j.heliyon.2024.e29066
Sivaraman, G. K., Gupta, S. S., Visnuvinayagam, S., Muthulakshmi, T., Elangovan, R., Perumal, V., Balasubramanium, G., Lodha, T., & Yadav, A. (2022). Prevalence of S. aureus and/or MRSA from seafood products from Indian seafood products. BMC Microbiology, 22(1), 233. https://doi.org/10.1186/s12866-022-02640-9
Tan, C. W., Rukayadi, Y., Hasan, H., Thung, T. Y., Lee, E., Rollon, W. D., Hara, H., Kayali, A. Y., Nishibuchi, M., & Radu, S. (2020). Prevalence and antibiotic resistance patterns of Vibrio parahaemolyticus isolated from different types of seafood in Selangor, Malaysia. Saudi Journal of Biological Sciences, 27(6), 1602–1608. https://doi.org/10.1016/j.sjbs.2020.01.002
Tate, H., Ayers, S., Nyirabahizi, E., Li, C., Borenstein, S., Young, S., Rice-Trujillo, C., Saint Fleurant, S., Bodeis-Jones, S., Li, X., Tobin-D’Angelo, M., Volkova, V., Hardy, R., Mingle, L., M’ikanatha, N. M., Ruesch, L., Whitehouse, C. A., Tyson, G. H., Strain, E., & McDermott, P. F. (2022). Prevalence of antimicrobial resistance in select bacteria from retail seafood—United States, 2019. Frontiers in Microbiology, 13. https://doi.org/10.3389/fmicb.2022.928509
Todd, E. C. D. (2014). Foodborne diseases: overview of biological hazards and foodborne diseases. In Encyclopedia of Food Safety (pp. 221–242). Elsevier. https://doi.org/10.1016/B978-0-12-378612-8.00071-8
Torell, E. C., Jamu, D. M., Kanyerere, G. Z., Chiwaula, L., Nagoli, J., Kambewa, P., Brooks, A., & Freeman, P. (2020). Assessing the economic impacts of post-harvest fisheries losses in Malawi. World Development Perspectives, 19, 100224. https://doi.org/10.1016/j.wdp.2020.100224
Toushik, S. H., Kim, K., Ashrafudoulla, M., Mizan, M. F. R., Roy, P. K., Nahar, S., Kim, Y., & Ha, S.-D. (2021). Korean kimchi-derived lactic acid bacteria inhibit foodborne pathogenic biofilm growth on seafood and food processing surface materials. Food Control, 129, 108276. https://doi.org/10.1016/j.foodcont.2021.108276
Wiradana, P. A., Permatasari, A. A. A. P., Sari, N. K. Y., Widhiantara, I. G., Sandhika, I. M. G. S., Waruwu, E., Fimaputra, J. M., Panjaitan, N. S. D., Putri, F. R., & Ansori, A. N. M. (2024). Isolation and molecular identification of endophytic fungi associated with brown algae for inhibiting Escherichia coli ESBL. Journal of Applied Veterinary Science And Technology, 5(1), 31–41. https://doi.org/10.20473/javest.V5.I1.2024.31-41
Wiradana, P. A., Sandhika, I. M. G. S., Widhiantara, I. G., Rizqy, A. N., Soegianto, A., & Yulianto, B. (2023). Contaminants and human health risks associated with exposure to microplastic ingestion of green mussels (Perna viridis) collected from The Kedonganan Fish Market, Bali. Jurnal Medik Veteriner, 6(2), 197–208. https://doi.org/10.20473/jmv.vol6.iss2.2023.197-208
Wiradana, P. A., Soegianto, A., Nege, A. S., & Naw, S. W. (2020). Pathogenic food-borne bacteria in Shellfish and shrimp from the largest traditional seafood market in Surabaya, Indonesia. Ecology, Environment and Conservation, 26(April Supplementary Issue), 1–6.
Wiradana, P. A., Yusup, D. S., & Soegianto, A. (2019). Biomonitoring Escherichia coli and coliform contamination in abalone (Haliotis squamata) cultivation pond in Musi Village, Gerokgak Sub-District, Buleleng-Bali. Aquacultura Indonesiana, 20(1), 32. https://doi.org/10.21534/ai.v20i1.143
Wirayudha, R. H., Herawati, D., Kusnandar, F., & Nurhayati, T. (2022). Kapasitas antioksidan dan sifat fisikokimia hidrolisat kolagen dari kulit ikan tuna sirip kuning dengan metode ultrasound assisted enzymatic reaction. Jurnal Pengolahan Hasil Perikanan Indonesia, 25(3), 393-404. http://dx.doi.org/10.17844/jphpi.v25i3.43325
Xie, T., Yu, Q., Tang, X., Zhao, J., & He, X. (2020). Prevalence, antibiotic susceptibility and characterization of Vibrio parahaemolyticus isolates in China. FEMS Microbiology Letters, 367(16). https://doi.org/10.1093/femsle/fnaa136
Yanestria, S. M., Rahmaniar, R. P., Wibisono, F. J., & Effendi, M. H. (2019). Detection of invA gene of Salmonella from milkfish (Chanos chanos) at Sidoarjo wet fish market, Indonesia, using polymerase chain reaction technique. Veterinary World, 12(1), 170–175. https://doi.org/10.14202/vetworld.2019.170-175
Yennie, Y., Dewanti-Hariyadi, R., Kusumaningrum, H. D., & Poernomo, A. (2022). Kontaminasi Staphylococcus aureus dan Bacillus cereus pada Susyi di Tingkat Ritel di Wilayah Jabodetabek. Jurnal Pengolahan Hasil Perikanan Indonesia, 25(2), 331-344. http://dx.doi.org/10.17844/jphpi.v25i2.42066
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