Peningkatan tekstur produk berbasis protein ikan dengan polifenol dari daun zaitun (Olea europaea L.) Improved textural properties of fish protein product with olive (Olea europaea L.) leaf polyphenol

Muh Ali Arsyad, Masahiro Ogawa

Abstract

Polyphenols from natural materials such as plants have been widely used to improve the texture of ingredients and food products. Olive leaves have a very high polyphenol content. This study aims to determine the effect of feeding with the addition of olive leaves on the protein structure of meat and the physical properties of surimi gel. Fish were kept separately based on feed (control, olive leaf addition, and olive leaf extract addition). Physical characteristics of surimi gel added with olive leaf meal (TDZ) and olive leaf extract meal (TEDZ) were determined by measuring hardness and chewiness. Fish fed olive leaf meal for 14 days had 1.1 times higher acid-soluble collagen content than fish meat fed without olive leaf meal. The hardness of surimi gel with the addition of TDZ increased by 38%, and the addition of TEDZ flour increased by 23%. The suppleness of the surimi gel added with TDZ was 1.52 times higher than the surimi gel without the addition of olive leaves, while the gel added with TEDZ had a suppleness 1.48 times higher than the surimi gel without the addition of olive leaves. Feeding with additional olive leaves can improve meat texture through increasing the quantity of collagen protein. Research has shown that adding olive leaves to surimi enhances the physical properties of the resulting gel. 

References

Albertos, I., Martín-Diana, A. B., Jaime, I., Avena-Bustillos, R. J., McHugh, T. H., Takeoka, G. R., Dao, L., & Rico, D. (2018). Antioxidant effect of olive leaf powder on fresh Atlantic horse mackerel (Trachurus trachurus) minced muscle. Journal of Food Processing and Preservation, 42(1), 1–8. https://doi.org/10.1111/jfpp.13397

Alcaide, E. M., & Nefzaoui, A. (1996). Recycling of olive oil by-products: Possibilities of utilization in animal nutrition. International Biodeterioration & Biodegradation, 38(3–4), 227–235. https://doi.org/10.1016/S0964-8305(96)00055-8

Armenta López, G.E.,Sumaya Martínez, M.T., Spanopoulos Hernández, M., Balois Morales, R., Sánchez Herrera, M., Jiménez Ruíz, E. (2015). Inclusion of natural antioxidant compounds in fish feeds to counteract oxidative stress. Revista Bio Ciencias, 3(2), 68-78. https://doi.org/10.15741/REVBIO.03.02.01

Arsyad, M. A., Akazawa, T., Nozaki, C., Yoshida, M., Oyana, K., Mukai, T., & Ogawa, M. (2018). Effects of olive leaf powder supplemented to fish feed on muscle protein of red sea bream. Fish Physiology and Biochmestry, 44(5), 1299–1308. https://doi.org/10.1007/s10695-018-0521-1

Arsyad, M. A., Akazawa, T., & Ogawa, M. (2018). Effects of olive leaf powder on mechanical properties of heat-induced surimi gel. Journal of Aquatic Food Product Technology, 28, 2-13. https://doi.org/10.1080/10498850.2018.1559904

Arsyad MA, Rusli A. 2020. Profil protein daging ikan kurisi merah (Pagrus major) yang diberi pakan dengan tambahan tepung daun zaitun (Olea europaea L.). Jurnal Pengolahan Hasil Perikanan Indonesia, 23(1), 93-102. https://doi.org/10.17844/jphpi.v23i1.30890

Arsyad, M. A., Rusli, A., & Ogawa, M. (2019). Sifat mekanik gel surimi yang ditambahkan olive leaf extract powder. Jurnal Pengolahan Hasil Perikanan Indonesia, 22(2), 318–326. https://doi.org/10.17844/jphpi.v22i2.27772

Balange, A., & Benjakul, S. (2009). Enhancement of gel strength of bigeye snapper (Priacanthus tayenus) surimi using oxidised phenolic compounds. Food Chemistry, 113(1), 61–70. https://doi.org/10.1016/j.foodchem.2008.07.039

Balange, A. K., & Benjakul, S. (2009). Effect of oxidised phenolic compounds on the gel property of mackerel (Rastrelliger kanagurta) surimi. LWT - Food Science and Technology, 42(6), 1059–1064. https://doi.org/10.1016/j.lwt.2009.01.013

Blanco, M., Vázquez, J. A., Pérez-martín, R. I., & Sotelo, C. G. (2019). Collagen extraction optimization from the skin of the small-spotted catshark (S . canicula) by response surface methodology. Marine Drug, 1–14. https://doi.org/10.3390/md17010040

Barik, S.K., Banerjee, S., Bhattacharjee, S., Gupta, S.K.D., Mohanty, S., & Mohanty, B.P. (2013). Proteomic analysis of sarcoplasmic peptides of two related fish species for food authentication. Applied Biochemistry and Biotechnology, 171, 1011–1021. https://doi.org/10.1007/S12010-013-0384-Y

Botsoglou, E., Govaris, A., Ambrosiadis, I., Fletouris, D., & Papageorgiou, G. (2014). Effect of olive leaf (Olea europea L.) extracts on protein and lipid oxidation in cooked pork meat patties enriched with n-3 fatty acids. Journal of the Science of Food and Agriculture, 94(2), 227–234. https://doi.org/10.1002/jsfa.6236

Botsoglou, E., Govaris, A., Christaki, E., & Botsoglou, N. (2010). Effect of dietary olive leaves and/or α-tocopheryl acetate supplementation on microbial growth and lipid oxidation of turkey breast fillets during refrigerated storage. Food Chemistry, 121(1), 17–22. https://doi.org/10.1016/j.foodchem.2009.11.083

Cavalheiro, C. V., Rosso, V. D., Paulus, E., Cichoski, A. J., Wagner, R., Menezes, C. R. de, & Barin, J. S. (2014). Composição química de folhas de oliveira (Olea europaea L.) da região de Caçapava do Sul, RS. Ciência Rural, 44(10), 1874–1879. https://doi.org/10.1590/0103-8478cr20131139

Dorey, R. C., & Draves, J. A. (1998). Spectrophotometric determination of total protein-biuret Method. Quantitative Analysis Laboratory: A New Approach Funded by the National Science Foundation, 48(3), 1–3.

Galal, S. A., Mousa, M. M., Elzanfaly, E. S., Hussien, E. M., Amer, E. A., & Zaazaa, H. E. (2024). Quantitative analysis of residual butylated hydroxytoluene and butylated hydroxyanisole in Salmo salar, milk, and butter by liquid chromatography–tandem mass spectrometry. Food Chemistry, 453, 139653. https://doi.org/10.1016/j.foodchem.2024.139653

Ge, L., Xu, Y., Xia, W., Zhao, N., & Jiang, Q. (2018). Contribution of myofibril filament disassembly to textural deterioration of ice-stored grass carp fillet: Significance of endogenous proteolytic activity, loss of heat shock protein and dephosphorylation of myosin light chain. Food Chemistry, 269, 511–518. https://doi.org/10.1016/j.foodchem.2018.07.047

H. Nakagawa, M. Sato, D.M. Gatlin III. (2007). Dietary Supplements for the Health and Quality of Cultured Fish. Page 244. CABI; Illustrated edition

Janairo, G., Sy, M. L., Yap, L., & Llanos-lazaro, N. (2020). Determination of the sensitivity range of biuret test for undergraduate biochemistry experiments determination of the sensitivity range of biuret test for undergraduate biochemistry experiments. eJournal Sci Technol, 6(5), 77-83.

Khan, N., & Mukhtar, H. (2007). Tea polyphenols for health promotion. Life Sciences, 81(7), 519–533. https://doi.org/10.1016/j.lfs.2007.06.011

Kliment, C. R., Englert, J. M., Crum, L. P., & Oury, T. D. (2011). Original article a novel method for accurate collagen and biochemical assessment of pulmonary tissue utilizing one animal. Int J Clin Exp Pathol, 4(4), 349–355.

Konno, K., Imamura, K., & Yuan, C.H. (2011). Myosin denaturation and cross-linking in alaska pollack salted surimi during its preheating process as affected by temperature. Food Science and Technology Research, 17(5), 423-428. https://doi.org/10.3136/fstr.17.423

Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685.

Lee, B., Kim, J. Y., Ryu, Y.-C., Lee, K., & Choi, Y. M. (2023). Research Note: Expression levels of collagen-related genes in PSE conditions and white striping features of broiler pectoralis major muscle. Poultry Science, 102(4), 102471. https://doi.org/10.1016/j.psj.2022.102471

Lertwittayanon, K., Benjakul, S., Maqsood, S., & Encarnacion, A.B. (2013). Effect of different salts on dewatering and properties of yellowtail barracuda surimi. International Aquatic Research, 5(10), 1-12. https://doi.org/10.1186/2008-6970-5-10

Marzoni, M., Chiarini, R., Castillo, A., Romboli, I., De Marco, M., & Schiavone, A. (2014). Effects of dietary natural antioxidant supplementation on broiler chicken and Muscovy duck meat quality. Animal Science Papers and Reports, 32(4), 359–368.

Magdalena, Mika., Anna, Antończyk., Agnieszka, Wikiera. (2023). Influence of synthetic antioxidants used in food technology on the bioavailability and metabolism of lipids - in vitro studies. Polish Journal of Food and Nutrition Sciences, 73(1), 95-107. https://doi.org/10.31883/pjfns/161366

Mkaouar, S., Krichen, F., Bahloul, N., Allaf, K., & Kechaou, N. (2018). Enhancement of bioactive compounds and antioxidant activities of olive (Olea europaea L.) leaf extract by instant controlled pressure drop. Food and Bioprocess Technology, 11, 1222–1229. https://doi.org/10.1007/s11947-018-2098-1

Moreno, H. M, Montero ,M. P., Gómez-Guillén, M. C., Fernández-Martín, F., Mørkøre, T., & Borderías J. (2012). Collagen characteristics of farmed Atlantic salmon with firm and soft fillet texture. Food Chemistry, 134(2), 678-85. https://doi.org/10.1016/j.foodchem.2012.02.160

Muzolf-Panek, M., Zaworska-Zakrzewska, A., Czech, A., Lisiak, D., & Kasprowicz-Potocka, M. (2024). Antioxidative status and meat quality traits as affected by dietary supplementation of finishing pigs with natural phenolics. Antioxidants. 13(11), 1362. https://doi.org/10.3390/antiox13111362

Nowsad, A. A., Kanoh, S., & Niwa, E. (1993). Aggregation of myosin heavy chain while grinding surimi and setting its paste. Nippon Suisan Gakkaishi, 59(11), 1-2.

Nozaki, C., Hibi, K., Akazawa, T., Nishiyama, T., Oyama, K., Yoshida, M., Mukai, T., Hayakawa, S., & Ogawa, M. (2020). Efects of feeding olive leaf powder on the chewiness of yellowtail meat. Nippon Suisan Gakkaishi, 86, 483–493.

Ogawa, M., Inoue, M., Hayakawa, S., O’Charoen, S., and Ogawa, M. (2017). Effects of rare sugar d-allulose on heat-induced gelation of surimi prepared from marine fish. Journal Science and Food Agriculture, 97(14), 5014-5020. https://doi.org/10.1002/jsfa.8381

Oyama, K., Tochino, M., Ueta, Y., Takemori, H., & Tada, T. (2010). Effects of a diet supplemented with powdered olive olea europaea leaf on prevention of dark muscle discoloration in cultured yellowtail Seriola quinqueradiata. Aquaculture Science, 58(2), 279–287.

Rahmawati, H., Agustini, T. W., Dewi, E. N., & Trianto, A. (2024). Karakteristik hidrolisat kolagen kulit ikan tenggiri kering dengan enzim papain. Jurnal Pengolahan Hasil Perikanan Indonesia, 27(12), 1156-1171. http://dx.doi.org/10.17844/jphpi.v27i12.55831

Ronald, B., Young., Debra, M., Moriarity., Christopher, E., McGee. (1986). Structural analysis of myosin genes using recombinant DNA techniques. Journal of Animal Science, http://dx.doi.org/10.2527/JAS1986.631259X

Rimoin, D.L., Cohn, D.H. & Eyre, D. (1994). Clinical-molecular correlations in the skeletal dysplasias. Pediatric Radiology, 24, 425-426. https://doi.org/10.1007/BF02011909

Shubina, V. S., Kobyakova, M. I., Penkov, N. V., Mitenko, G. V., Udaltsov, S. N., & Shatalin, Yu. V. (2024). Two novel membranes based on collagen and polyphenols for enhanced wound healing. International Journal of Molecular Sciences, 25(22), 12353. https://doi.org/10.3390/ijms252212353

Tang, Z., He, Y., Zhang, J., Zhao, Z., Nie, Y.-Q., & Zhao, X. (2024). Study on the structural changes of boneless chicken claw collagen and its effect on water retention performance. Foods, 13(22), 3682. https://doi.org/10.3390/foods13223682

Taşbozan, O., & Erbaş, C. (2023). Antioxidant additives in fish feeds. Black Sea Journal of Agriculture, 6(3), 321-325. https://doi.org/10.47115/bsagriculture.1246497

Yanqin, Lu., Shie, Zhang., Shie, Zhang., Yanzhou, Wang., Xiuzhi, Ren., Jinxiang, Han., Jinxiang, Han. (2019). Molecular mechanisms and clinical manifestations of rare genetic disorders associated with type I collagen. Intractable & Rare Diseases Research, 8(2), 98-107. https://doi.org/10.5582/IRDR.2019.01064

Authors

Muh Ali Arsyad
muh.aliarsyadabdullah@gmail.com (Primary Contact)
Masahiro Ogawa
Arsyad M. A., & Ogawa M. (2025). Peningkatan tekstur produk berbasis protein ikan dengan polifenol dari daun zaitun (Olea europaea L.): Improved textural properties of fish protein product with olive (Olea europaea L.) leaf polyphenol. Jurnal Pengolahan Hasil Perikanan Indonesia, 28(2), 130-141. https://doi.org/10.17844/jphpi.v28i2.61826

Article Details