Potensi aktivitas antioksidan ekstrak spons laut Stylissa carteri Potential antioxidant activity of sea sponge Stylissa carteri extract

Murad Alvian K Adewal, Asadatun Abdullah, Neviaty Putri Zamani, Dondy Arafat, Inna Puspa Ayu, Beginer Subhan

Abstract

The sea sponge Stylissa carteri plays an important role because it is one of the constituent biota of coastal and marine ecosystems, especially in coral reef ecosystems. The increasing utilization of bioactive compounds in sea sponges is feared to significantly reduce the population of sea sponges. The prevention effort involves cultivating sea sponges for sustainable utilization. This study aims to determine the antioxidant activity of transplanted Stylissa carteri sponges and those that live naturally in nature. This study was conducted in Pramuka Island using the sponge explant combination method. The three types of transplants were number one (SO), number two (S. carteri and Aaptos suberitoides), and number three (S. carteri, A. suberitoides, and Acropora hard coral). Antioxidant activity was tested with three repetitions on three transplanted and naturally occurring S. carteri sponges (SA) using the DPPH (1,1-Diphenyl-2-Picrylhydrazyl) method. The results indicated that the four sample sources contained strong antioxidant activity, namely SO had an IC50 value of 86.22±23.6 μg/mL, SAP 86.58±30.7 μg/mL, SAC 86.10±23.4 μg/mL, and natural living SA 54.27±16.9 μg/mL. The transplanted S. carteri sea sponge has strong antioxidant activity and can be an alternative solution in terms of raw material availability for the development of potential pharmaceutical products.

References

Amato, A., Esposito, R., Federico, S., Pozzolini, M., Giovine, M., Bertolino, M., Guida, M., Manfra, L., Libralato, G., Zupo, V., & Costantini, M. (2023). Marine sponges as promising candidates for integrated aquaculture combining biomass increase and bioremediation: an updated review. In Frontiers in Marine Science, 10 (1234225), 1-16. https://doi.org/10.3389/fmars.2023.1234225

Bergman, O., Haber, M., Mayzel, B., Anderson, M. A., Shpigel, M., Hill, R. T., & Ilan, M. (2011). Marine-based cultivation of diacarnus sponges and the bacterial community composition of wild and maricultured sponges and their larvae. Marine Biotechnology, 13(6), 1169–1182. https://doi.org/10.1007/s10126-011-9391-6

Chamidah, A., Afrilia, H. C., Ahmad, M. G., & Arisandi, D. (2024). Isolasi klorofil a dan analisis aktivitas antioksidan dari mikroalga C. vulgaris. Jurnal Pengolahan Hasil Perikanan Indonesia, 27(11), 1006-1020. http://dx.doi.org/10.17844/jphpi.v27i11.57470

Carter, E. (2018). State of the sea: Indonesia, volume one: an overview of Marine resource management for small-scale fisheries and critical Marine habitats in Indonesia. Jakarta: Ministry of Marine Affairs and Fisheries (MMAF), Republic of Indonesia and USAID Sustainable Project, Ecosystems Advanced (SEA).

Denny, R., Yudistira, R., & Mpila, D. A. (2022). Uji aktivitas antioksidan ekstrak etanol spons Stylissa Carteri dari Pulau Mentehage Minahasa Utara. Jurnal PHARMACON, 11(1), 1309–1314. https://doi.org/10.35799/pha.11.2022.39142

Duckworth, A. R., Battershill, C. N., & Bergquist, P. R. (1997). Influence of explant procedures and environmental factors on culture success of three sponges. Aquaculture, 156 (3–4), 251-267. https://doi.org/10.1016/S0044-8486(97)00131-2

Duckworth, A. (2009). Farming sponges to supply bioactive metabolites and bath sponges: a review. Mar. Biotechnol, 11(6), 669-79. http://dx.doi.org/10.1007/s10126-009-9213-2

Haedar, Sadarun, B., & Palupi, R. D. (2016). Potensi keanekaragaman jenis dan sebaran spons di Perairan Pulau Saponda Laut Kabupaten Konawe. Jurnal Sapa Laut, 1(1), 1-9. http://dx.doi.org/10.33772/jsl.v1i1.923

Herdhiansyah, R., Zetra Y., & Nugraheni, Z, V. (2015) Senyawa lipid spons Haliclona cymaeformis sebagai biomarka dan aktivitasnya terhadap mikroba. Jurnal Sains dan Seni, 4(2), C133-138. https://doi.org/10.12962/J23373520.V4I2.14011

Sankar, R. K., Chadha, N. K., Roy, D. S., Banerjee, P., Saharan, N., & Krishnan, P. (2016). Growth and survival of marine sponges, Stylissa massa (carter, 1887) and Liosina paradoxa (thiele, 1899) in sea and land based culture systems. Indian Journal of Fisheries, 63(4), 55–60. http://dx.doi.org/10.21077/ijf.2016.63.4.43906-09

Krisnawati, S., Adithya, Y, Irma, A. (2021). Uji aktivitas antioksidan ekstrak etanol spons (Stylissa sp.) yang dikoleksi dari Teluk Manado. Jurnal PHARMACON, 10(1), 756–761. https://doi.org/10.35799/pha.10.2021.32773

Liang, J., She, J., Fu, J., Wang, J., Ye, Y., Yang, B., & Tao, H. (2023). Advances in natural products from the marine sponge associated microorganisms with antimicrobial activity in the last decade. Marine Drugs, 21(4), 236. https://doi.org/10.3390/md21040236

Lumempow, F., Yudistira, A., & Juliana Suoth, E. (2023). Uji aktivitas antioksidan ekstrak dan fraksi spons stylissa carteri yang diperoleh dari Pulau Manado Tua. Pharmacy Medical Journal, 6(1), 1-7. https://doi.org/10.35799/pmj.v6i1.43086

Maisaroh, D. S., Hanif, A. Y. A., Munir, M., & Sa’adah, N. (2023). Uji ekstrak spons laut jenis Ptilocaulis marquezii dari Perairan Kendit sebagai potensi antibakteri Escherichia coli. Journal of Marine Research, 12(1), 161–166. https://doi.org/10.14710/jmr.v12i1.36278

Marzuki, I. (2018). Eksplorasi Spons Indonesia: Seputar Kepulauan Spermonde. PT Penerbit Nas Media Pustaka

Maslin, M., Gaertner-Mazouni, N., Debitus, C., de Voogd, N. J., & Ho, R. (2021). Marine sponge aquaculture towards drug development: An ongoing history of technical, ecological, chemical considerations and challenges. In Aquaculture Reports, 21, 100813. https://doi.org/10.1016/j.aqrep.2021.100813

Maxs, M., Bengen, D. G., Abdullah, A., Elfahmi, Syafrizayanti, Andirani, Y., Arafat, D., Bashari, M. H., Akbar, N., & Subhan, B. (2024). Survival, growth, and antioxidant activity of the marine sponge Aaptos suberitoides from three variations of transplantation treatment. Egyptian Journal of Aquatic Biology and Fisheries, 28(6), 1313–1330. https://doi.org/10.21608/ejabf.2024.396340

Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology. 26(2), 211-219.

Page, M. J., Northcote, P. T., Webb, V. L., Mackey, S., Handley, S. J. (2005). Aquaculture trials for th production of biologically active metabolites in the New Zealand sponge Mycale hentscheli (Demospongiae: Poecilosclerida). Aquaculture, 250(1–2), 256–269. https://doi.org/10.1016/j.aquaculture.2005.04.069

Pallela, R., & Ehrlich, H. (2016). Marine sponges: chemicobiological and biomedical applications. in marine sponges: Chemicobiological and Biomedical Applications, pp. 1–381. http://dx.doi.org/10.1007/978-81-322-2794-6

Peck, M., Tapilatu, R. F., Kurniati, E., & Rosado, C. (2021). Rapid coral reef assessment using 3D modelling and acoustics: acoustic indices correlate to fish abundance, diversity and environmental indicators in West Papua, Indonesia. PeerJ, 9, e10761. http://dx.doi.org/10.7717/peerj.10761

Razak, T. B., Boström-Einarsson, L., Alisa, C. A. G., Vida, R. T., & Lamont, T. A. (2022). Coral reef restoration in Indonesia: A review of policies and projects. Marine Policy, 137, 104940. http://dx.doi.org/10.1016/j.marpol.2021.104940

Rikantara, F. S., Utami, M. R., & Kasasiah, A. (2022). Aktivitas antioksidan kombinasi ekstrak daun sirsak (Annona muricata L.) dan ekstrak daun pepaya (Carica papaya L.) dengan Metode DPPH. Lumbung Farmasi, Jurnal Ilmu Kefarmasian, 3(2), 124-133. https: //doi.org/10.31764/lf.v3i2.8819

Rozanah, A., Febrianingsih, R. I., Larasati, A. O., & Hadisaputri, Y. E. (2023). Cultivation method optimation of demospongiae sponge and its symbiont bacteria. Indonesian Journal of Biological Pharmacy Literarute Review, 3(1) 33-41. doi:10.24198/ijbp.v3i1.39865

Rumagit, H. M., Runtuwene, M. R., & Sudewi, S. (2015). Uji fitokimia dan uji aktivitas antioksidan dari ekstrak etanol spons Lamellodysidea herbacea. PHARMACON Jurnal Ilmiah Farmasi, 4(3), 183–192. https://doi.org/10.35799/pha.4.2015.8858

Salikode, J. S., Yudistira, A., & Hariyanto, Y. A. (2024). Uji aktivitas antioksidan ekstrak etanol spons Aaptos aaptos yang diperoleh dari Pantai Selatan Kabupaten Minahasa. PHARMACON, 13(1), 431–437. https://doi.org/10.35799/pha.13.2024.49266

Sibarani, S. I., Yudistira, A., & Mpila, D. A. (2020). Uji aktivitas antioksidan spons stylissa sp. dengan menggunakan metode DPPH (1,1-difenil-2-pikrilhidrazil). PHARMACON, 9(3), 419–424. https://doi.org/10.35799/pha.9.2020.30027

Trianto, A., Ridhlo, A., Triningsih, D. W., & Tanaka, J. (2017). Study on anticancer activity of extracts of sponges collected from Biak Water, Indonesia. IOP Conference Series: Earth and Environmental Science, 55(1). https://doi.org/10.1088/1755-1315/55/1/012069

Widiawati, & Asih, E. N. A. (2024). Potensi skrining fitokimia dan aktivitas antioksidan ekstrak daun Avicennia marina dan Avicennia alba dari Selat Madura. Jurnal Pengolahan Hasil Perikanan Indonesia, 27(5), 393-406. http://dx.doi.org/10.17844/jphpi.v27i5.52421

Authors

Murad Alvian K Adewal
Asadatun Abdullah
Neviaty Putri Zamani
Dondy Arafat
Inna Puspa Ayu
Beginer Subhan
beginersubhan@apps.ipb.ac.id (Primary Contact)
Adewal M. A. K., Abdullah A., Zamani N. P., Arafat D., AyuI. P., & Subhan B. (2025). Potensi aktivitas antioksidan ekstrak spons laut Stylissa carteri: Potential antioxidant activity of sea sponge Stylissa carteri extract . Jurnal Pengolahan Hasil Perikanan Indonesia, 28(3), 245-253. https://doi.org/10.17844/jphpi.v28i3.60270

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