Purifikasi fikosianin dari Spirulina platensis hasil intervensi kemangi (Ocimum basilicum) pada konsentrasi amonium sulfat berbeda Purification of phycocyanin from Spirulina platensis with basil (Ocimum basilicum) intervention on different ammonium sulphate concentration

Yuliani Yuliani, Tri Winarni Agustini, Eko Nurcahya Dewi, Diana Nur Afifah


Spirulina platensis is a filamentous cyanobacterium that contains phycobiliprotein compounds. Phycocyanin is the most prevalent phycobiliprotein pigment-protein complex and possesses the greatest commercial value in the food, cosmetic, and pharmaceutical sectors.  The specific activity of phycocyanin can be enhanced through purification, which aims to increase its level of purity. Basil, scientifically known as Ocimum basilicum, is a plant that possesses azulene, a blue aromatic hydrocarbon. The purpose of this study was to investigate the impact of basil intervention and saturation level of ammonium sulfate deposition on the purity index, phycocyanin content, recovery, and total protein in the S. platensis phycocyanin purification process.  The purification method utilized for phycocyanin involves a multistep process, which includes the extraction of S. platensis without any intervention or basil intervention, followed by precipitation with ammonium sulfate at different saturation levels ranging from 0-20%, 20-50%, 50-70%, and 70-90%, and ultrafiltration using DF/UF.  The findings indicated that the optimal saturation level for samples without intervention at the purification stage involving ammonium sulfate precipitation was 50%, whereas for samples with intervention, the optimal level was 70%.  The findings revealed that the phycocyanin obtained through basil intervention exhibited superior purity and phycocyanin content compared to the samples that did not undergo intervention at every stage of purification.  The purity value at the final stage of purification was 2.54 AU for the samples that did not receive any intervention, and 2.57 AU for the basil intervention samples. Basil intervention treatment has been shown to significantly decreased the recovery value by 47.39% and the total phycocyanin protein by 12.5% in samples without intervention. Additionally, the intervention or non-intervention treatment of basil leaves on S. platensis reduced the recovery value and total protein content at each purification stage.


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Yuliani Yuliani
yuliyani234@gmail.com (Primary Contact)
Tri Winarni Agustini
Eko Nurcahya Dewi
Diana Nur Afifah
YulianiY., AgustiniT. W., DewiE. N., & AfifahD. N. (2023). Purifikasi fikosianin dari Spirulina platensis hasil intervensi kemangi (Ocimum basilicum) pada konsentrasi amonium sulfat berbeda: Purification of phycocyanin from Spirulina platensis with basil (Ocimum basilicum) intervention on different ammonium sulphate concentration. Jurnal Pengolahan Hasil Perikanan Indonesia, 26(3), 448-459. https://doi.org/10.17844/jphpi.v26i3.46208

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