Kandungan senyawa fitokimia dan aktivitas antifungal ekstrak Padina sp. menggunakan ultrasound assisted extraction terhadap Aspergillus flavus Screening of phytochemical compounds and antifungal activity of Padina sp. extracted by ultrasound assisted extraction against Aspergillus flavus

Nur Hidayah, I Ketut Sumandiarsa, Walian Maimun Alqadiri

Abstract

Brown algae, Padina sp., possess phytochemical elements that exhibit antifungal properties. This study aimed to determine the best solvent based on the active compound components and antifungal activity of the Padina sp. extract against Aspergillus flavus. The extraction procedure was conducted using an ultrasonic wave-assisted method that involved the use of three solvents: ethanol, methanol, and ethyl acetate. The parameters assessed were the yield percentage, phytochemicals, and antifungal activity. Phytochemical screening was conducted qualitatively, and the antifungal activity against A. flavus was evaluated using the agar well diffusion method. The waters surrounding Panggang Island served as the sampling location for Padina sp., which exhibited a pH of 9, temperature ranging from 28.1°C to 28.9°C, salinity of 31-32 ppm, and dissolved oxygen (DO) level of 6.5 ppm. The ethanol, methanol, and ethyl acetate extracts yielded 20.98±1.54%, while the methanol extract yielded 13.71 ± 5.94 % and 6.67 ± 3.30% of the total yield, respectively.The Padina sp. extract contained various phytochemical compounds, including alkaloids, saponins, steroids, and phenols, when extracted with three different solvents; the methanol extract exhibited the most potent antifungal activity, with an inhibitory zone diameter of 7.21±0.41 mm at a concentration of 30%. Conversely, the ethyl acetate extract at 10% concentration showed the smallest inhibitory zone diameter of 1.17±0.09 mm. Padina sp. extract, obtained from the waters surrounding Panggang Island, demonstrates antifungal activity against A. flavus in the medium category, as evidenced by the presence of four distinct phytochemical compounds.

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Authors

Nur Hidayah
hidaits.hidayah10@gmail.com (Primary Contact)
I Ketut Sumandiarsa
Walian Maimun Alqadiri
HidayahN., SumandiarsaI. K., & AlqadiriW. M. (2024). Kandungan senyawa fitokimia dan aktivitas antifungal ekstrak Padina sp. menggunakan ultrasound assisted extraction terhadap Aspergillus flavus: Screening of phytochemical compounds and antifungal activity of Padina sp. extracted by ultrasound assisted extraction against Aspergillus flavus. Jurnal Pengolahan Hasil Perikanan Indonesia, 27(4), 297-308. https://doi.org/10.17844/jphpi.v27i4.44634

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