DNA Barcoding untuk Autentikasi Produk Hiu Segar dari Perairan Nusa Tenggara Barat DNA Barcoding for Fresh Shark Products Authentication from West Nusa Tenggara Waters
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Accepted
12 January 2022
Published
25 January 2022
Keywords:
-
bioinformatics, CITES, DNA barcoding
Abstract
Shark and ray fisheries in Indonesia, including Tanjung Luar, West Nusa Tenggara, face considerable challenges where shark and ray populations continue to decrease. Sharks are particularly vulnerable to overfishing and extinction due to slow sexual maturity and low fecundity. Shark meat and fin are generally in demand by consumers; however, other shark products are not separately recorded in trade statistics, making them difficult to identify based on morphological indicators. As an alternative, the DNA barcode method can be applied. This study aims to identify rare shark species recorded on CITES that have landed at the port of NTB using the DNA barcoding method. The research includes the stages of DNA isolation, DNA amplification, sequencing and bioinformatics analysis. The results of sequencing and bioinformatics analysis showed Sphyrna lewini and Carcharhinus falciformis DNA species in samples with 99-100% similarity. These results show that the use of sharks that are included in the CITES list is still often carried out, so it is necessary to implement stricter regulations and transfer public knowledge so that endangered shark species can be preserved.
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Steinke D, Bernard AM, Horn RL, Hilton P, Hanner R, Shivji MS. 2017. DNA analysis of traded shark fins and mobulid gill plates reveals a high proportion of species of conservation concern. Scientific Reports. 7(1):9505.
Velez-Zuazo X, Alfaro-Shigueto J, Mangel J, Papa R, Agnarsson I. 2015. What barcode sequencing reveals about the shark fishery in Peru. Fisheries Research. 161:34–41.
Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PDN. 2005. DNA barcoding Australia’s fish species. Philosophical Transactions of the Royal Society B. 360(1462):1847–1857.
Wong EH-K, Shivji MS, Hanner RH. 2009. Identifying sharks with DNA barcodes: assessing the utility of a nucleotide diagnostic approach. Molecular Ecology Resources. 9(s1):243–256.
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. Journal of Molecular Biology. 215(3):403–410.
Appleyard SA, White WT, Vieira S, Sabub B. 2021. Publisher Correction: Artisanal shark fishing in Milne Bay Province, Papua New Guinea: biomass estimation from genetically identified shark and ray fins. Scientific Reports. 11(1):17962.
Barbuto M, Galimberti A, Ferri E, Labra M, Malandra R, Galli P, Casiraghi M. 2010. DNA barcoding reveals fraudulent substitutions in shark seafood products: The Italian case of “palombo” (Mustelus spp.). Food Research International 43(1):376–381.
Bräutigam A, Callow M, Campbell IR, Camhi MD, Cornish AS, Dulvy NK, Fordham SV, Fowler SL, Hood AR, McClennen C, Reuter EL, Sant G, Simpfendorfer CA, Welch DJ. 2016. Global Priorities for Conserving Sharks and Rays A 2015–2025 Strategy.
Cardeñosa D, Fields AT, Shea SKH, Feldheim KA, Chapman DD. 2020. Relative contribution to the shark fin trade of Indo-Pacific and Eastern Pacific pelagic thresher sharks. Animal Conservation, siap terbit.
Chuang PS, Hung TC, Chang HA, Huang CK, Shiao JC. 2016. The species and origin of shark fins in Taiwan’s fishing ports, markets, and customs detention: A DNA barcoding analysis. PLoS One. 11(1):1–13.
Dent F, Clarke S. 2015. State of the global market for shark products. FAO Fish and Aquacultures Technical Papers 590, siap terbit.
Drancourt M, Bollet C, Carlioz A, Martelin R, Gayral JP, Raoult D. 2000. 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. Journal of Clinical Microbiology. 38(10):3623–3630.
Dudgeon CL, Blower DC, Broderick D, Giles JL, Holmes BJ, Kashiwagi T, Krück NC, Morgan JAT, Tillett BJ, Ovenden JR. 2012. A review of the application of molecular genetics for fisheries management and conservation of sharks and rays. Journal of Fish Biology. 80(5):1789–1843.
Ferretti F, Worm B, Britten GL, Heithaus MR, Lotze HK. 2010. Patterns and ecosystem consequences of shark declines in the ocean. Ecology Letters. 13(8):1055–1071.
Fields AT, Abercrombie DL, Eng R, Feldheim K, Chapman DD. 2015. A novel mini-DNA barcoding assay to identify processed fins from internationally protected shark species. PLoS One. 10(2):1–10.
Hanner RH, Naaum AM, Shivji MS. 2016. Conclusion: DNA-Based Authentication of Shark Products and Implications for Conservation and Management. Elsevier Inc.
Hellberg RS, Isaacs RB, Hernandez EL. 2019. Identification of shark species in commercial products using DNA barcoding. Fisheries Research. 210 April 2018:81–88.
Isda MN, Chaidamsari T. 2013. Analisis sekuen gen proteinase inhibitor (TcPIN) terkait dengan ketahanan terhadap penggerek buah kakao. Prosiding Semirata FMIPA Universitas Lampung.
Kumar S, Tamura K, Jakobsen IB, Nei M. 2001. MEGA2: molecular evolutionary genetics analysis software. Bioinformatics. 17(12):1244–1245.
Madduppa H, Putri ASP, Wicaksono RZ, Subhan B, Akbar N, Ismail F, Arafat D, Prabuning D, Sani LMI, Srimariana ES, et al. 2020. Morphometric and DNA barcoding of endemic halmaheran walking shark (Hemiscyllium halmahera, Allen, 2013) in North Maluku, Indonesia. Biodiversitas. 21(7):3331–3343.
Marshall LJ, Barone M. 2016. SharkFin Guide: identifying sharks from their fins.
Muttaqin E, Simeon BM, Ichsan M, Dharmadi, Prasetyo AP, Booth H, Yulianto I, Friedman K. 2018. The scale, value and importance of non-fin shark and ray commodities in Indonesia. Food and Agriculture Organization of the United Nations, Ministry of Marine Affairs and Fisheries, Wildlife Conservation Society.
O’Bryhim JR, Parsons ECM, Lance SL. 2017. Forensic species identification of elasmobranch products sold in Costa Rican markets. Fisheries Research. 186:144–150.
Rodrigues-Filho LF, Pinhal D, Sodré D, Vallinoto M. 2012. Shark DNA Forensics: Applications and Impacts on Genetic Diversity. Analysis of Genetic Variation in Animals. Mahmut Caliskan (ed.).
Sanger F, Nicklen S, Coulson AR. 1977. DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America. 74(12):5463–5467.
Sembiring A, Pertiwi NPD, Mahardini A, Wulandari R, Kurniasih EM, Kuncoro AW, Cahyani NKD, Anggoro AW, Ulfa M, Madduppa H, Carpenter KE, Barber PH, Mahardika GN. 2015. DNA barcoding reveals targeted fisheries for endangered sharks in Indonesia. Fisheries Research. 164:130–134.
Simeon BM, Agustina S, Muttaqin E, Yulianto I, Ichsan M, Muhsin. 2017. Laporan teknis: Pemantauan hasil tangkapan hiu dan pari di Tanjung Luar, Provinsi Nusa Tenggara Barat. Mei 2021:14.
Steinke D, Bernard AM, Horn RL, Hilton P, Hanner R, Shivji MS. 2017. DNA analysis of traded shark fins and mobulid gill plates reveals a high proportion of species of conservation concern. Scientific Reports. 7(1):9505.
Velez-Zuazo X, Alfaro-Shigueto J, Mangel J, Papa R, Agnarsson I. 2015. What barcode sequencing reveals about the shark fishery in Peru. Fisheries Research. 161:34–41.
Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PDN. 2005. DNA barcoding Australia’s fish species. Philosophical Transactions of the Royal Society B. 360(1462):1847–1857.
Wong EH-K, Shivji MS, Hanner RH. 2009. Identifying sharks with DNA barcodes: assessing the utility of a nucleotide diagnostic approach. Molecular Ecology Resources. 9(s1):243–256.
Authors
Alsere Bardian SahabaM., AbdullahA., & NugrahaR. (2022). DNA Barcoding untuk Autentikasi Produk Hiu Segar dari Perairan Nusa Tenggara Barat: DNA Barcoding for Fresh Shark Products Authentication from West Nusa Tenggara Waters . Jurnal Pengolahan Hasil Perikanan Indonesia, 24(3), 425-432. https://doi.org/10.17844/jphpi.v24i3.38318
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