Aktivitas Antioksidan dan Total Fenolik Rumput Laut Gracilaria sp. Kabupaten Brebes Antioxidant Activity and Total Phenolic Of Seaweed Gracilaria sp. from Brebes
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Accepted
26 August 2021
Published
31 August 2021
Keywords:
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Gracilaria sp, Antioxidant Activity, Total Phenolic
Abstract
Brebes regency is one of the seaweed producing areas in Central Java province with an area of seaweed ponds as much as 4,350 ha of the total pond area of 12,748 ha that produces 200 tons of dried seaweed per month. Seaweed can be used as a source of antioxidants because it contains bioactive compounds especially for phenolic compounds, flavonoids and alkaloids. The purpose of this study is to examine the antioxidant activity and total phenolic powder of seaweed Gracilaria sp. from Brebes. The observed parameters are free radical capture capacity using DPPH radical scavenging activity (RSA) method and total phenolic using Folin-Ciocalteu method. This study used a Complete RandomIzed Design (RAL) consisting of 2 treatments (seaweed powder from fresh and dry raw materials) and conducted 3 repetitions. Gracilaria sp. Seaweed powder from fresh raw materials has a total phenol content and a higher free radical catching capacity compared to dry raw materials. The total content of seaweed powder phenol Gracilaria sp. from Brebes district is about 4.35–4.49 (mg GAE/100 g) and its antioxidant activity is about 15.90–42.50 (%RSA). There are differences in the capture capacity of free radicals using wet and dry seaweed raw materials.
References
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Chew YL, Lim YY, Omar M, Khoo KS. 2008. Antioxidant activity of three edible seaweeds from two areas in South East Asia. LWT-Food Science and Technology. 41(6):1067-1072.
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Munandar A, Surilayani D, Haryati S, Sumantri MH, Aditia RP, Pratama G. 2019. Characterization flour of two seaweeds (Gracilaria spp. and Kappaphycus alvarezii) for reducing consumption of wheat flour in Indonesia. IOP Conference Series: Earth and Environmental Science. 383 (2019) 012009.
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Sheikh TZB, Yong CL, Lian MS. 2009. In vitro antioxidant activity of the hexane and methanolic extracts of Sargassum baccularia and Cladophora patentiramea. Journal of Applied Sciences. 13(9): 2490-2493.
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Uju, Santoso J, Ramadhan W, Abrory MF. 2018. Extraction of native agar from Gracillaria sp with ultrasonic acceleration at low temperature. Jurnal Pengolahan Hasil Perikanan Indonesia. 21(3):414-422.
White PAS, Oliveira RCM, Oliveira AP, Serafini MR, Araújo AAS, Gelain Moreira DPJCF, Almeida JRGS, Quintans JSS, Quintan - Junior LJ, Santos MRV. 2014. Antioxidant activity and mechanisms of action of natural compounds isolated from lichens: A Systematic review. Molecules. 19:14496-14527.
Winarti C, Nurdjanah N. 2005. Peluang tanaman rempah dan obat sebagai sumber pangan fungsional. Jurnal Litbang Pertanian. 24(2): 47-55.
Yakhin LA, Wijaya KM, Santoso J. 2013. Peningkatan kualitas gel sosis ikan lele dengan penambahan tepung Gracilaria gigas. Jurnal Pengolahan Hasil Perikanan Indonesia. 16(2):177-182.
Yudiati E, Ridho A, Nugroho AA, Sedjati S, Maslukah L. 2020. Analisis kandungan agar, pigmen dan proksimat rumput laut Gracilaria sp. pada reservoir dan biofilter tambak udang Litopenaeus vannamei. Buletin Oseanografi Marina. 9(2): 133-140.
Zubia M, Robledo D, Freile-Pelegin Y. 2007. Antioxidant activities in marine macroalgae from the coasts of Quintana Roo and Yucatan, Mexico. Journal of Applied Phycology. 19(5):449-458
Adri D, Hersoelistyorini W. 2013. Aktivitas antioksidan dan sifat organoleptik teh daun sirsak (Annona muricata Linn.) berdasarkan variasi lama pengeringan.Jurnal Pangan dan Gizi. 4(1): 1-12.
Chaidir A. 2006. Kajian rumput laut sebagai sumber serat alternatif untuk minuman berserat. [Tesis]. Bogor: Institut Pertanian Bogor.
Chew YL, Lim YY, Omar M, Khoo KS. 2008. Antioxidant activity of three edible seaweeds from two areas in South East Asia. LWT-Food Science and Technology. 41(6):1067-1072.
Dinas Kelautan dan Perikanan Kabupaten Brebes. 2016. Laporan Statistika Produksi Perikanan Budidaya Pembenihan dan Nilainya. Brebes: Dinas Kelautan dan Perikanan Kabupaten Brebes.
Dwiyitno D. 2010. Seaweed as a potential source of dietary fiber. Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology. 6(1): 9-17.
Ganesan P, Kumar CS, Bhaskar N. 2008. Antioxidant properties of methanol extract and its solvent fractions obtained from selected Indian red seaweeds. Bioresource Technology. 99(8): 2717-2723.
Hikmah AF, Budhiyanti SA, Ekantari N. 2009. Pengaruh pengeringan terhadap aktivitas antioksidan Spirulina platensis. Prosiding Seminar Nasional Tahunan VI Hasil Penelitian Perikanan dan Kelautan. (4):1-11).
Husni A, Putra DR, Lelana IYB. 2014. Aktivitas antioksidan Padina sp. pada berbagai suhu dan lama pengeringan. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 9(2): 165-173.
Jahangiri Y, Ghahremani H, Torghabeh JA, Salehi EA. 2011. Effect of temperature and solvent on the total phenolic compounds extraction from leaves of Ficus carica. Journal of Chemical and Pharmaceutical Research. 3(5):253-259.
Julyasih KSM, Wirawan IGP, Harijani WS, Widajati W. 2009. Aktivitas antioksidan beberapa jenis rumput laut (seaweeds) komersial di Bali. Prosiding Seminar Akselerasi Pengembangan Teknologi Pertanian dalam Mendukung Revitalisasi Pertanian. Fakultas Pertanian dan LPPM UPN Veteran.
Kaimudin M, Moniharapon T, Mailoa MN, Pattipeilohy F. 2020. Efektivitas ekstrak Gracilaria sp. dalam menghambat pertumbuhan mikroba ikan layang dan tuna pada penyimpanan suhu ruang. Jurnal Pengolahan Hasil Perikanan Indonesia. 23(3): 523-531.
Kedare SB, Singh RP. 2011. Genesis and development of DPPH method of antioxidant assay. Journal of Food Science and Technology. 48(4): 412–422.
Kumar SR, Hosokawa M, Miyashita K. 2013. Fucoxanthin: A marine carotenoid exerting anti-cancer effects by affecting multiple mechanisms. Marine drugs. 11(12): 5130-5147.
Molyneux, P., 2004. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology. 26(2): 211-219.
Munandar A, Surilayani D, Haryati S, Sumantri MH, Aditia RP, Pratama G. 2019. Characterization flour of two seaweeds (Gracilaria spp. and Kappaphycus alvarezii) for reducing consumption of wheat flour in Indonesia. IOP Conference Series: Earth and Environmental Science. 383 (2019) 012009.
Nawaly H, Susanto AB, Uktolseja JL. 2013. Senyawa bioaktif dari rumput laut sebagai antioksidan. Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning . 10(1):
Purwaningsih S, Deskawati E. 2020. Karakteristik dan aktivitas antioksidan rumput laut Gracilaria sp. asal Banten. Jurnal Pengolahan Hasil Perikanan Indonesia. 23(3): 503-512.
Salamah E, Erungan AC, Retnowati Y. 2006. Pemanfaatan Gracilaria sp. dalam pembuatan permen jelly. Jurnal Pengolahan Hasil Perikanan Indonesia. 9(1):
Sanger G, Kaseger BE, Rarung LK, Damongilala L. 2018. Potensi beberapa jenis rumput laut sebagai bahan pangan fungsional, sumber pigmen dan antioksidan alami. Jurnal Pengolahan Hasil Perikanan Indonesia. 21(2): 208-217.
Sheikh TZB, Yong CL, Lian MS. 2009. In vitro antioxidant activity of the hexane and methanolic extracts of Sargassum baccularia and Cladophora patentiramea. Journal of Applied Sciences. 13(9): 2490-2493.
Sudarmadji S, Bambang H, Suhardi. 2007. Analisis Bahan Makanan dan Pertanian. Liberty. Yogyakarta. ID
Tamat SR, Wikanta T, Maulina LS. 2007. Aktivitas antioksidan dan toksisitas senyawa bioaktif dari ekstrak rumput laut hijau Ulva reticulata Forsskal. Jurnal Ilmu Kefarmasian Indonesia. 5(1):31-36.
Uju, Santoso J, Ramadhan W, Abrory MF. 2018. Extraction of native agar from Gracillaria sp with ultrasonic acceleration at low temperature. Jurnal Pengolahan Hasil Perikanan Indonesia. 21(3):414-422.
White PAS, Oliveira RCM, Oliveira AP, Serafini MR, Araújo AAS, Gelain Moreira DPJCF, Almeida JRGS, Quintans JSS, Quintan - Junior LJ, Santos MRV. 2014. Antioxidant activity and mechanisms of action of natural compounds isolated from lichens: A Systematic review. Molecules. 19:14496-14527.
Winarti C, Nurdjanah N. 2005. Peluang tanaman rempah dan obat sebagai sumber pangan fungsional. Jurnal Litbang Pertanian. 24(2): 47-55.
Yakhin LA, Wijaya KM, Santoso J. 2013. Peningkatan kualitas gel sosis ikan lele dengan penambahan tepung Gracilaria gigas. Jurnal Pengolahan Hasil Perikanan Indonesia. 16(2):177-182.
Yudiati E, Ridho A, Nugroho AA, Sedjati S, Maslukah L. 2020. Analisis kandungan agar, pigmen dan proksimat rumput laut Gracilaria sp. pada reservoir dan biofilter tambak udang Litopenaeus vannamei. Buletin Oseanografi Marina. 9(2): 133-140.
Zubia M, Robledo D, Freile-Pelegin Y. 2007. Antioxidant activities in marine macroalgae from the coasts of Quintana Roo and Yucatan, Mexico. Journal of Applied Phycology. 19(5):449-458
Authors
MasrikhiyahR. (2021). Aktivitas Antioksidan dan Total Fenolik Rumput Laut Gracilaria sp. Kabupaten Brebes: Antioxidant Activity and Total Phenolic Of Seaweed Gracilaria sp. from Brebes . Jurnal Pengolahan Hasil Perikanan Indonesia, 24(2), 236-242. https://doi.org/10.17844/jphpi.v24i2.34020
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