Aktivitas Antioksidan Caulerpa sp. Segar dan Rebus Antioxidant Activity of Fresh and Boiled Caulerpa sp.
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Accepted
19 January 2021
Published
4 February 2021
Keywords:
-
DPPH, extraction, free radical, latoh
Abstract
Free radical is a molecule that is highly reactive and unstable, can cause damage to proteins, Deoxyribose Nucleic Acid (DNA), and cell membranes, and can trigger a degenerative diseases. The damages can be prevented by natural or synthetic antioxidant compounds. Caulerpa sp. a green seaweed as a potential source of natural antioxidants. Caulerpa sp. commonly processed into “urap” seaweed in fresh and boiled condition. The boiling process may change the antioxidant activity, so it is necessary to research on the antioxidant activity in Caulerpa sp. fresh and boiled. Extraction method used was a single extraction using methanol p.a for 1×24 hours. Testing of antioxidant activity using DPPH method. Caulerpa sp. fresh and boiled extract has a yield of 8.88% and 6.44%. IC50 values Caulerpa sp. fresh amounted to 452.37±8.29 mg/L and boiled amounted to 484.59±5.69 mg/L. IC50 value category is classified as very weak by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method, so it needs to do another antioxidant activity assay methods and purification of crude extract of Caulerpa sp..
References
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Deepa N, Kaur C, George B, Singh B, Kapoor HC. 2007. Antioxidant constituents in some sweet pepper (Capsicum annuum L.) genotypes during maturity. LWT - Food Science and Technology 40(1): 121-129.
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Jebakumar AZ, Hassan SN, Siju KG, Manoj G. 2012. Natural anti-oxidants and in-vitro metohds for anti-oxidant activity. International Journal of Pharmacology Research 2(1): 46-55.
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Nurjanah, Azka A, Abdullah A. 2012. Aktivitas antioksidan dan komponen bioaktif semanggi air (Marsilea crenata). Jurnal Inovasi dan Kewirausahaan 1(3): 152-158.
Nurjanah, Jacoeb AM, Hidayat T, Shylina A. 2015. Bioactive compounds and antioxidant activity of lindur stem bark (Bruguiera gymnorrhiza). International Journal of Plant Science and Ecology 1(5):182-189.
Nurjanah, Jacoeb AM, Nugraha R, Permatasari M, Sejati TKA. 2014. Perubahan komposisi kimia, aktivitas antioksidan, vitamin c dan mineral tanaman genjer (Limnocharis flava) akibat pengukusan. Jurnal Inovasi dan Kewirausahaan 3(3): 185-195.
Pramesti R. 2013. Aktivias antioksidan ekstrak rumput laut Caulerpa serrulata dengan metode DPPH (1,1 definil 2 pikrilhidrazil). Buletin Oseanografi Marina 2(2): 7-15.
Purwaningsih S. 2012. Aktivitas antioksidan dan komposisi kimia keong matah merah (Cerithidea obtusa). Jurnal Ilmu Kelautan 17(1):39-48.
Ratana P, Chirapart A. 2006. Nutritional evaluation of tropical green seaweeds Caulerpa lentillifera and Ulva reticulata. Kasetsart Journal (National Science) 40(1): 75-83.
Salazar-Aranda R, Perez-Lopes LA, Lopez-Arroyo J, Alanis-Garza BA, Waksman de Torres N. 2011. Antimicrobial and antioxidant activities of plants from Northeast of Mexico. Evidence-Based Complementary and Altenative Medicine 1(1): 1-6.
Santoso J, Anwariyah S, Rumiantin RO, Putri AP, Ukhty N, Yoshie Y. Phenol content, antioxidant activity and fibers profile of four tropical seagrasses from Indonesia. Journal of Coastal Development 15(2): 189-196.
Setyaningsih D, Apriyantono A, Sari MP. 2010. Analisis Sensori untuk Industri Pangan dan Agro. Bogor (ID): IPB Press.
Sipayung MY, Suparmi, Dahlia. 2015. Pengaruh suhu pengukusan terhadap sifat fisika kimia tepung ikan rucah. Jurnal Pengolahan Unri 2(1): 1-13.
Sudirman S, Nurjanah, Jacoeb AM. 2014. Proximate compositions, bioactive compounds and antioxidant activity from large-leafed mangrove (Bruguiera gymnorrhiza) fruit. International Food Research Journal 21(6): 2387-2391.
Sultana V, Baloch GN, Ambreen AJ, Tariq MR, Ehteshamul-Haque S. 2011. Comparative efficacy of a red alga Solieria robusta, chemical fertilizers and ZZ pesticides in managing the root diseases and growth of soybean. Pakistan Journal of Botany 43(1): 1-6.
Susilawati I. 2007. Kajian metode pengasapan dalam pengolahan dendeng batokok produk khas sumatera barat. [tesis]. Bogor (ID): Sekolah Pascasarjana Teknologi Pasca Panen, Institut Pertanian Bogor.
Suter IK, Wijaya IMAS, Yusa NM. 2011. Formulasi ledok instan yang ditambahkan ikan tongkol dan rumput laut. Jurnal Teknologi dan Industri Pangan 22(2): 190-196.
Tamat SR, Wikanta T, Maulina LS. 2007. Aktivitas antioksidan dan toksisitas senyawa bioaktif dari ekstrak rumput laut hijau Ulva reticulata Forsskal. Jurnal Ilmu Kefarmasian Indonesia 5(1): 31-36.
Udayaprakash NK, Ranjithkumar M, Deepa S, Sripriya N, Al-Arfaj AA, Bhuvaneswari S. 2015. Antioxidant, free radical scavenging and GC-MS composition of Cinnamomum iners. Industrial Crops and Products 69(3): 175-178.
Usmiati S, Nurdjannah N. 2007. Pengaruh lama perendaman dan cara pengeringan terhadap mutu lada putih. Jurnal Pertanian Indonesia 16(3): 91-98.
Wicaksono GS, Zubaidah E. 2015. Pengaruh karagenan dan lama perebusan daun sirsak terhadap mutu dan karakteristik jelly drink daun sirsak. Jurnal Pangan dan Agroindustri 3(1): 281-291.
Winarno FG. 2008. Kimia Pangan dan Gizi. Bogor (ID): M-Brio Press.
Yunizal, Murtini JT, Dolaria N, Purdiwoto B, Abdulrokhim, Carkipan. 1998. Prosedur Analisis Kimiawi Ikan dan Produk Olahan Hasil-Hasil Perikanan. Jakarta (ID): Pusat Penelitian dan Pengembangan Perikanan.
[AOAC] Association of Official Analitycal Chemist. 2005. Official Method of Analysis of The Association of Official Analytical of Chemist. Virginia (US): Association of Official Analytical Chemist, Inc.
Aryudhani N. 2007. Kandungan senyawa fenol rumput laut Caulerpa racemosa dan aktivitas antioksidannya. [skripsi]. Bogor (ID): Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor.
Atmadja WS, Kadi A, Sulistijo, Rachmaniar. 1996. Pengenalan Jenis-Jenis Rumput Laut Indonesia. Jakarta (ID): Puslitbang Oseanologi-LIPI.
[BSN]. Badan Standardisasi Nasional. 1995. SNI 01-0222-95 Bahan Tambahan Makanan. Jakarta (ID): Badan Standardisasi Nasional.
Chew KK, Ng SY, Thoo YY, Khoo MZ, Wan Aida WM, Ho CW. 2011. Effect of ethanol concentration, extraction time and extraction temperature on the recovery of phenolic compounds andantioxidant capacity of Centella asiatica extracts. International Food Research Journal 18: 566-573.
Deepa N, Kaur C, George B, Singh B, Kapoor HC. 2007. Antioxidant constituents in some sweet pepper (Capsicum annuum L.) genotypes during maturity. LWT - Food Science and Technology 40(1): 121-129.
Farasat M, Khavari-Nejad RA, Nabavi SMB, Namjooyan F. 2014. Antioxidant activity, total phenolics and flavonoid contents of some edible green seaweeds from northern coasts of the persian gulf. Iranian Journal of Pharmaceutical Research 13(1): 163-170.
Indriastuti ATD, Setiyono, Erwanto Y. 2012. Pengaruh jus daun sirih sebagai bahan pracuring dan lama penyimpanan terhadap komposisi kimia dan angka peroksida dendeng ayam petelur. Agrinimal 2(1): 1-5.
Jebakumar AZ, Hassan SN, Siju KG, Manoj G. 2012. Natural anti-oxidants and in-vitro metohds for anti-oxidant activity. International Journal of Pharmacology Research 2(1): 46-55.
Kasim S. 2013. Pengaruh konsentrasi natrium hidroksida terhadap rendemen karaginan yang diperoleh dari rumput laut jenis Eucheuma spinosum asal kota bau-bau. Jurnal Farmasi dan Farmakologi 17(1): 1-8.
Kumar A, Kumari SN, Bhargavan D. 2012. Evaluation of in vitro antioxidant potential of ethanolic extract from the leaves of Achyranthes aspera. Asian Journal of Pharmaceutical and Clinical Research 5(3): 146-148
Kumar M, Vishal G, Puja K, Reddy CRK, Jha B. 2011. Assessment of nutrient composition and antioxidant potential of Caulerpaceae seaweeds. Journal of Food Composition and Analysis 24(1): 270-278.
Ma’ruf WF, Ratna I, Eko ND, Eko S, Ulfah A. 2013. Profil rumput laut Caulerpa racemosa dan Gracilaria verrucosa sebagai edible food. Jurnal Saintek Perikanan 9(1): 68-74.
Matanjun P, Mohamed S, Mustapha NM, Muhammad K. 2009. Nutrient content of tropical edible seaweeds, Eucheuma cottonii, Caulerpa lentillifera, and Sargassum polycystum. Journal of Applied Phycology 21(1): 75–80.
Maulida R. 2007. Aktivitas antioksidan rumput laut Caulerpa lentillifera. [skripsi]. Bogor (ID): Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor.
Megayana Y, Subekti S, Alamsjah MA. 2012. Studi kandungan alginate dan klorofil rumput laut Sargassum sp. pada umur panen yang berbeda. Journal of Aquaculture and Fish Health 1(1): 120-127.
Molyneux P. 2004. The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Journal Science of Technology 26(2): 211-219.
Nguyen VT. Ueng J, Tsai G. 2011. Proximate composition, total phenolic content, and antioxidant activity of seagrape (Caulerpa lentillifera). Journal of Food Science 76(7): 950-958.
Nugroho M. 2012. Pengaruh suhu dan lama ekstraksi secara pengukusan terhadap rendemen dan kadar albumin ikan gabus (Ophiocephalus striatus). Jurnal Teknologi Pangan 3(1): 64-75.
Nurjanah, Nurimala M, Hidayat T, Sudirjo F. 2016. Characteristics of seaweed as raw materials for cosmetics. Aquatic Procedia. 7:177-180.
Nurjanah, Jacoeb AM, Asmara DA, Hidayat T. Phenolic compound of fresh and boiled sea grapes (Caulerpa sp.) From tual, maluku. Food Scien Tech Journal. 1(1):31-39
Nurjanah. Jacoeb AM. Hidayat T. Chrystiawan R. 2018. Perubahan Komponen Serat Rumput Laut Caulerpa sp (dari Tual Maluku) akibat proses perebusan. Jurnal Ilmu Teknologi Kelautan Tropis 10 (1): 35-48.
Nurjanah, Azka A, Abdullah A. 2012. Aktivitas antioksidan dan komponen bioaktif semanggi air (Marsilea crenata). Jurnal Inovasi dan Kewirausahaan 1(3): 152-158.
Nurjanah, Jacoeb AM, Hidayat T, Shylina A. 2015. Bioactive compounds and antioxidant activity of lindur stem bark (Bruguiera gymnorrhiza). International Journal of Plant Science and Ecology 1(5):182-189.
Nurjanah, Jacoeb AM, Nugraha R, Permatasari M, Sejati TKA. 2014. Perubahan komposisi kimia, aktivitas antioksidan, vitamin c dan mineral tanaman genjer (Limnocharis flava) akibat pengukusan. Jurnal Inovasi dan Kewirausahaan 3(3): 185-195.
Pramesti R. 2013. Aktivias antioksidan ekstrak rumput laut Caulerpa serrulata dengan metode DPPH (1,1 definil 2 pikrilhidrazil). Buletin Oseanografi Marina 2(2): 7-15.
Purwaningsih S. 2012. Aktivitas antioksidan dan komposisi kimia keong matah merah (Cerithidea obtusa). Jurnal Ilmu Kelautan 17(1):39-48.
Ratana P, Chirapart A. 2006. Nutritional evaluation of tropical green seaweeds Caulerpa lentillifera and Ulva reticulata. Kasetsart Journal (National Science) 40(1): 75-83.
Salazar-Aranda R, Perez-Lopes LA, Lopez-Arroyo J, Alanis-Garza BA, Waksman de Torres N. 2011. Antimicrobial and antioxidant activities of plants from Northeast of Mexico. Evidence-Based Complementary and Altenative Medicine 1(1): 1-6.
Santoso J, Anwariyah S, Rumiantin RO, Putri AP, Ukhty N, Yoshie Y. Phenol content, antioxidant activity and fibers profile of four tropical seagrasses from Indonesia. Journal of Coastal Development 15(2): 189-196.
Setyaningsih D, Apriyantono A, Sari MP. 2010. Analisis Sensori untuk Industri Pangan dan Agro. Bogor (ID): IPB Press.
Sipayung MY, Suparmi, Dahlia. 2015. Pengaruh suhu pengukusan terhadap sifat fisika kimia tepung ikan rucah. Jurnal Pengolahan Unri 2(1): 1-13.
Sudirman S, Nurjanah, Jacoeb AM. 2014. Proximate compositions, bioactive compounds and antioxidant activity from large-leafed mangrove (Bruguiera gymnorrhiza) fruit. International Food Research Journal 21(6): 2387-2391.
Sultana V, Baloch GN, Ambreen AJ, Tariq MR, Ehteshamul-Haque S. 2011. Comparative efficacy of a red alga Solieria robusta, chemical fertilizers and ZZ pesticides in managing the root diseases and growth of soybean. Pakistan Journal of Botany 43(1): 1-6.
Susilawati I. 2007. Kajian metode pengasapan dalam pengolahan dendeng batokok produk khas sumatera barat. [tesis]. Bogor (ID): Sekolah Pascasarjana Teknologi Pasca Panen, Institut Pertanian Bogor.
Suter IK, Wijaya IMAS, Yusa NM. 2011. Formulasi ledok instan yang ditambahkan ikan tongkol dan rumput laut. Jurnal Teknologi dan Industri Pangan 22(2): 190-196.
Tamat SR, Wikanta T, Maulina LS. 2007. Aktivitas antioksidan dan toksisitas senyawa bioaktif dari ekstrak rumput laut hijau Ulva reticulata Forsskal. Jurnal Ilmu Kefarmasian Indonesia 5(1): 31-36.
Udayaprakash NK, Ranjithkumar M, Deepa S, Sripriya N, Al-Arfaj AA, Bhuvaneswari S. 2015. Antioxidant, free radical scavenging and GC-MS composition of Cinnamomum iners. Industrial Crops and Products 69(3): 175-178.
Usmiati S, Nurdjannah N. 2007. Pengaruh lama perendaman dan cara pengeringan terhadap mutu lada putih. Jurnal Pertanian Indonesia 16(3): 91-98.
Wicaksono GS, Zubaidah E. 2015. Pengaruh karagenan dan lama perebusan daun sirsak terhadap mutu dan karakteristik jelly drink daun sirsak. Jurnal Pangan dan Agroindustri 3(1): 281-291.
Winarno FG. 2008. Kimia Pangan dan Gizi. Bogor (ID): M-Brio Press.
Yunizal, Murtini JT, Dolaria N, Purdiwoto B, Abdulrokhim, Carkipan. 1998. Prosedur Analisis Kimiawi Ikan dan Produk Olahan Hasil-Hasil Perikanan. Jakarta (ID): Pusat Penelitian dan Pengembangan Perikanan.
Authors
HidayatT., Nurjanah, Mardiono JacoebA., & Adhitia PuteraB. (2021). Aktivitas Antioksidan Caulerpa sp. Segar dan Rebus : Antioxidant Activity of Fresh and Boiled Caulerpa sp . Jurnal Pengolahan Hasil Perikanan Indonesia, 23(3), 566-575. https://doi.org/10.17844/jphpi.v23i3.33869
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