Pengaruh Ekstrak Daun Mimba (Azadirachta indica) terhadap Histamin Daging Ikan Tongkol Abu The Effectiviness of Neem Leaves (Azadirachta indica) to Longtail Tuna Meat Histamine
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Accepted
10 August 2021
Published
23 August 2021
Keywords:
-
histamine, neem leaves, longtail tuna
Abstract
Longtail tuna (Thunnus tonggol) as scrombidae family which has high histamine contain that can cause poisoning for consumers. Some attempts can decrease histamine contain, one of them are use mimba leaves (Azadirachta indica). The research aimed to determine the effectiveness of neem leaves extract giving towards tongkol fish histamine. Neem leaves extraction use maceration method as long as 2 days. Longtail tuna sampel that measured of length is 30 cm which kept on room temperature as long as 5 hours used as sample research. The used treatment was immersion of neem leaves extract with concentration 5%, 25%, and 50%. Histamine analysis use HPLC device and data tabulation with SPSS program. The research result show the histamine contain of control treatment, 5%; 25%; and 50% respectively was 60.03, 49.63, 50.54, and 53.81 ppm. SPSS test results has indicate that giving of 5% neem leaves extract concentration to tongkol fish is more effective to prevent the increasing of tongkol fish histamine contain. Neem leaves extract concentration which 5%, 25%, and 50% respectively can decrease longtail tuna fish histamine contain with the effectiviness value was 17.15%, 15.80%, and 10.36%.
References
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Soraya C, Sunnati FW. 2019. Efek antibakteri ekstrak daun mimba (Azadirachta indica) terhadap pertumbuhan Enterococcus faecalis secara in-vitro. Cakradonya Dent Journal. 11(1). 23–32.
Sulistiawati S. 2014. Analisis histamin pada berbagai jenis ikan pelagis dengan metode High Performance Liquid Chromatography (HPLC). [Skripsi]. Pangkep: Politeknik Pertanian Negeri Pangkajene dan Kepulauan.
Supriyanto, Simon BW, Rifa IM, Yunianta. 2017. Uji fitokimia dan aktivitas antioksidan ekstrak daun mimba (Azadirachta indica Juss). Prosiding SNATIF ke-4. 523–529.
Susmitha S, Vidyamol KK, Ranganayaki P, Vijayaragavan R. 2013. Phytochemical extraction and antimicrobial properties of Azadirachta indica (neem). Global Journal of Pharmacology. 7(3): 316-320.
Wei CI, Chen JA, Koburger JA, Otwell WS, Marshall MR. 1990. Bacterial growth and histamine production on vacuum packaged tuna. Jurnal Food Sciene. 55: 59-63.
Wodi SIM, Wini T, Mala N. 2018. Histamin dan identifikasi bakteri pembentuk histamin pada tuna mata besar (Thunnus obesus). Jurnal Teknologi Perikanan dan Kelautan. 9 (2): 185-192.
Astuti I, Asniati N. 2018. Pengaruh ekstrak daun belimbing wuluh terhadap histamin pada ikan cakalang (Katsuwonus pelamis). Gorontalo Fisheries Journal. 1(2): 1–9.
Ayini U, Siti HB, Titis CD. 2014. Efek antibakteri ekstrak daun mimba (Azadirachta indica A. Juss) terhadap bakteri Vibrio algynoliticus secara in vitro. Journal of Biology & Biology Education. 6(1): 67-75.
Aziz NH, Farag SE, Mousa LA, Abozaid MA. 1998. Comparative antibacterial and antifungal eff ect of some phenolic compounds. Microbios. 93(374): 43-45.
[BSN] Badan Standardisasi Nasional. 2006. Petunjuk pengujian organoleptik dan atau sensori. SNI 01-2346.2006. Jakarta (ID): Badan Standardisasi Nasional.
Cahyaningsih E, Putu ESKY. 2020. Uji aktivitas ekstrak daun mimba (Azadirachta indica A. Juss) sebagai bahan pengawet alami buah tomat. Jurnal Ilmiah Medicamento. 6(2): 118-122.
de-Souza L, Wahidulla S, Devi P. 2010. Antibacterial phenolic from the mangrove Lumnitzera racemosa. Indian Journal of Marine Science. 39(2): 294-298.
[DKP] Dinas Kelautan dan Perikanan Provinsi Jawa Timur. 2018. Form Laut Triwulan 2018. Surabaya: DKP Jatim.
[FDA] Food and Drug Administration. 2011. Scombrotoxin (Histamine) formation di dalam: Fish and Fishery Product Hazards and Control Guide. Washington: Departemen of Health and Human Service, Center for Food Safety and Applied Nutrition.
Hiruma S, Ishihar M, Nakamura S, Sato Y, Asahina H, Fukuda K, Takayama T, Murakami K, Yokoe H. 2020. Bioshell calcium oxide-containing liquids as a sanitizer for the reduction of histamine production in Raw Japanese Pilchard, Japanese Horse Mackerel, and Chub Mackerel. Foods. 9(7): 1-12.
Juharni J. 2013. Pengaruh konsentrasi garam dan lama fermentasi terhadap kadar histamin peda ikan kembung perempuan (Rastrelinger nelectus). Agrikan: Jurnal Ilmiah Agribisnis dan Perikanan. 6(1): 73-80.
Junaedi AS, Riana F, Sari HCP, Witria, Zainuri M. 2020. Kualitas daging ikan kurisi (Nemipterus japonicus) hasil tangkapan Branta Fishing Port, Pamekasan. Jurnal Pengolahan Hasil Perikanan Indonesia. 23(2): 303-319.
Lee HS, Kim MJ. 2002. Selective responses of three ginkgo biloba leaf-derived constituents on human intestinal bacteria. Journal of Agricultural and Food Chemistry. 50: 1840-1844.
Milo MS, Ekawati LM, Pranata FS. 2011. Mutu ikan tongkol (Euthynnus affinis) di Kabupaten Gunung Kidul dan Sleman Daerah Istimewa Yogyakarta (ID): Fakultas Teknobiologi Universitas Atma Jaya Yogyakarta.
Prasetiawan NR, Agustini TW, Farid W. 2013. Penghambatan pembentukan histamin pada daging ikan tongkol (Euthynnus affinis) oleh quercetin selama penyimpanan histamine inhibition on mackerel (Euthynus affinis) flesh by using quercetine during storage. Jurnal Pengolahan Hasil Perikanan Indonesia. 16(2): 150–158.
Puspasari AR, Eko ND, Laras R. 2016. Aplikasi antioksidan dari ekstrak lamun (Cymodocea rotundata) pada minyak ikan tongkol (Euthynnus affinis). Agritech. 37(2): 115-120.
Radjawane C, Darmanto YS, Swastawati F. 2016. Kajian kandungan histamin ikan cakalang (Katsuwonus pelamis) segar dan asap pada sentral pengolahan ikan asap Di Kota Ambon. Prosiding Seminar Nasional Kelautan Universitas Trunojoyo Madura, pp. 316–320.
Raju GN, Sai KB, Meghana MS, Chandana K, Suresh PV,Nadendla RR. 2015. Synthesis, Characterization and Biological Activity of Indole-2-carboxylic acide derivatives. International Journal of Pharmaceutical Chemistry. 5: 202- 206.
Restiangsih YH, Thomas H. 2018. Analisis pertumbuhan dan laju eksploitasi ikan tongkol abu, Thunnus tonggol (Bleeker,1851) di Perairan Laut Jawa. Bawal. 10(2): 111-120.
Sandhar HK, Kumar B, Prasher S, Tiwari P, Salhan M, Sharma P. 2011. A review of phytochemistry and pharmacology of flavonoids. International Pharmaceutica Sciencia. 1(1): 25-41.
Sannomiya M, Michelin DC, Rodrigues CM, Santos LC, Salgado HRN, Hiruma-Lima CA, Brito ARSM, Vilegas W. 2005. Byrsonima Crassa Niedenzu (IK): Antimicrobial activity and chemical study. Journal of Basic and Applied Pharmaceutical Science. 26(1): 71-75.
Setyarini VD, Indah L, Christ K. 2019. Kadar histamin pada udang vannamei (Litopenaeus vannamei) dan identifikasi bakteri pembentuk histamin. Analis Kesehatan Sains. 8(1): 666-671.
Sitakar NM, Jamin, Faisal A, Mahdi M, Zakiah HNS. 2016. Pengaruh suhu pemeliharaan dan masa simpan daging ikan nila (Oreochromis niloticus) pada penyimpanan suhu -20 ℃ terhadap jumlah total bakteri. Jurnal Medika Veterinaria. 10(2): 162–165.
Soraya C, Sunnati FW. 2019. Efek antibakteri ekstrak daun mimba (Azadirachta indica) terhadap pertumbuhan Enterococcus faecalis secara in-vitro. Cakradonya Dent Journal. 11(1). 23–32.
Sulistiawati S. 2014. Analisis histamin pada berbagai jenis ikan pelagis dengan metode High Performance Liquid Chromatography (HPLC). [Skripsi]. Pangkep: Politeknik Pertanian Negeri Pangkajene dan Kepulauan.
Supriyanto, Simon BW, Rifa IM, Yunianta. 2017. Uji fitokimia dan aktivitas antioksidan ekstrak daun mimba (Azadirachta indica Juss). Prosiding SNATIF ke-4. 523–529.
Susmitha S, Vidyamol KK, Ranganayaki P, Vijayaragavan R. 2013. Phytochemical extraction and antimicrobial properties of Azadirachta indica (neem). Global Journal of Pharmacology. 7(3): 316-320.
Wei CI, Chen JA, Koburger JA, Otwell WS, Marshall MR. 1990. Bacterial growth and histamine production on vacuum packaged tuna. Jurnal Food Sciene. 55: 59-63.
Wodi SIM, Wini T, Mala N. 2018. Histamin dan identifikasi bakteri pembentuk histamin pada tuna mata besar (Thunnus obesus). Jurnal Teknologi Perikanan dan Kelautan. 9 (2): 185-192.
Authors
WitriaW., & ZainuriM. (2021). Pengaruh Ekstrak Daun Mimba (Azadirachta indica) terhadap Histamin Daging Ikan Tongkol Abu: The Effectiviness of Neem Leaves (Azadirachta indica) to Longtail Tuna Meat Histamine . Jurnal Pengolahan Hasil Perikanan Indonesia, 24(2), 209-217. https://doi.org/10.17844/jphpi.v24i2.33514
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